Filtered By:
Source: Gene

This page shows you your search results in order of date. This is page number 9.

Order by Relevance | Date

Total 132 results found since Jan 2013.

A novel siRNA validation system for functional screening of effective RNAi targets in mammalian cells and development of a derivative lentivirus delivery system.
Abstract RNA interference technology is a widely used tool for the regulation of gene expression at the post-transcriptional level. One major challenge is to find the effective short interfering (si)RNA for target gene rapidly and easily, and then to deliver the siRNA into cells or tissues with high efficiency. Here, we designed a novel siRNA validation vector using a dual luciferase reporter system for the functional screening of effective RNAi targets in mammalian cells. Then, based on a siRNA expression cassette, we developed a derivative lentivirus delivery system to infect the appropriate cells or tissues for...
Source: Gene - December 27, 2014 Category: Genetics & Stem Cells Authors: Huang G, Gao Q, Zhao Y, Dong Z, Li T, Guan X, Jiang J Tags: Gene Source Type: research

Alteration of BRCA1 expression affects alcohol-induced transcription of RNA Pol III-dependent genes.
Abstract Emerging evidence has indicated that alcohol consumption is an established risk factor for breast cancer. Deregulation of RNA polymerase III (Pol III) transcription enhances cellular Pol III gene production, leading to an increase in translational capacity to promote cell transformation and tumor formation. We have reported that alcohol intake increases Pol III gene transcription to promote cell transformation and tumor formation in vitro and in vivo. Studies revealed that tumor suppressors, pRb, p53, PTEN and Maf1 repress the transcription of Pol III genes. BRCA1 is a tumor suppressor and its mutation is...
Source: Gene - November 8, 2014 Category: Genetics & Stem Cells Authors: Zhong Q, Shi G, Zhang Y, Lu L, Levy D, Zhong S Tags: Gene Source Type: research

Adipose triglyceride lipase regulates lipid metabolism in dairy goat mammary epithelial cells.
In conclusion, these data suggest that the ATGL gene plays an important role in triglyceride lipolysis in GMECs and provides the first experimental evidence that ATGL may be involved in lipid metabolism during lactation. PMID: 25307872 [PubMed - as supplied by publisher]
Source: Gene - October 10, 2014 Category: Genetics & Stem Cells Authors: Li J, Luo J, Wang H, Shi H, Zhu J, Sun Y, Yu K, Yao D Tags: Gene Source Type: research

siRNA-mediated knockdown of CiGRP78 gene expression leads cell susceptibility to heavy metal cytotoxicity.
In this study, three most representative heavy metal ions, Pb(2+), Hg(2+) and Cd(2+), were used to stimulate Ctenopharyngodon idella kidney (CIK) cells. The results showed that cell viability under Pb(2+), Hg(2+) and Cd(2+) stress decreased significantly. The longer and greater concentrations of stimulation from heavy metal ions, the higher rate of cell death were observed. Among them, Hg(2+) is the most hazardous to cells. Under the same stress condition, Hg(2+) resulted in 50% of cell death, Cd(2+) (or Pb(2+)) led to 45% (or 35%) of cell death, respectively. Western immunoblotting indicated that C. idella GRP78 (CiGRP78)...
Source: Gene - September 18, 2014 Category: Genetics & Stem Cells Authors: Zhong B, Mao H, Fan Q, Liu Y, Hu Y, Mi Y, Wu F, Hu C Tags: Gene Source Type: research

Oroxylin A inhibits ATRA-induced IL-6 expression involved in retinoic acid syndrome by down-regulating CHOP.
Abstract Production of IL-6 constituted the major cause of death in the ATRA trial called retinoic acid syndrome (RAS). LAP and LIP are active and inactive isoforms of C/EBPβ, respectively. Inactive LIP dimerized with LAP to eliminate its activity. Following treatment with ATRA, CHOP expression was increased and dimerized with LIP more preferentially than LAP to rescue function of LAP. Oroxylin A has been reported to activate CHOP, a key mediator of unfolded protein response (UPR) pathway, and resulted in apoptosis. Interestingly, we found that low concentration of oroxylin A (≦40μM) showed no apoptosis effect...
Source: Gene - September 1, 2014 Category: Genetics & Stem Cells Authors: Hui H, Yang H, Dai Q, Wang Q, Yao J, Zhao K, Guo Q, Lu N Tags: Gene Source Type: research

The TLR3, PI3K, survin, FasL, Fas genes as major risk factors of occurrence and development of cervical cancer disease.
This study demonstrates that TLR3-siRNA and LY294002 treatment can markedly suppress cervical cancer cell invasion, tumor growth and increase survival life by silencing targeted genes. PMID: 25106857 [PubMed - as supplied by publisher]
Source: Gene - August 5, 2014 Category: Genetics & Stem Cells Authors: Liu HN, Shi HR, Zhao XL, Zhang RT, Liu GZ, Zhang JX Tags: Gene Source Type: research

The ways of action of long non-coding RNAs in cytoplasm and nucleus.
Abstract Over the past fifteen years, small regulatory RNAs, such as siRNA and miRNA, have been extensively investigated and the underlying molecular mechanisms have been well documented, suggesting that ncRNAs play a major function in many cellular processes. An expanding body of evidence reveals that long non-coding RNAs (lncRNAs), once described as dark matter, are involved in diverse cellular progresses, including regulation of gene expression, dosage compensation, genomic imprinting, nuclear organization and nuclear-cytoplasm trafficking via a number of complex mechanisms. The emerging links between lncRNAs a...
Source: Gene - June 23, 2014 Category: Genetics & Stem Cells Authors: Zhang K, Shi ZM, Chang YN, Hu ZM, Qi HX, Hong W Tags: Gene Source Type: research

Global expression profile of telomerase-associated genes in HeLa cells.
Abstract Telomerase is a specialized nucleoprotein enzyme complex that maintains the telomere length. The telomerase reverse transcriptase (TERT) is the catalytically active component of the telomerase complex. In humans, the protein component (hTERT) and RNA component (hTR) are found to differentially express in cancer cells. In contrast to differentiated cells, most of the cancer cells overexpress hTERT, which is needed to maintain the proliferative potential of cells. The overexpression of telomerase is not proportionate to telomere length in cancer cells, suggesting that the immortalizing phenotype can be medi...
Source: Gene - June 11, 2014 Category: Genetics & Stem Cells Authors: Varshney A, Ramakrishnan SK, Sharma A, Santosh B, Bala J, Yadava PK Tags: Gene Source Type: research

Sp1 transcriptionally regulates BRK1 expression in non-small cell lung cancer cells.
Abstract Following a previous study reporting that BRK1 is upregulated in non-small cell lung cancer (NSCLC), the present study sought to clarify the role of specificity protein 1 (Sp1) in the transcriptional regulation of the BRK1 gene. Therefore, a construct, named F8, consisting of the -1341 to -1nt sequence upstream of the start codon of the BRK1 gene inserted into pGL4.26 was made. A series of truncated fragments was then constructed based on F8. Segment S831, which contained the -84 to -1nt region, displayed the highest transcriptional activity in the A549, H1299 and H520 NSCLC cell lines. Bioinformatic anal...
Source: Gene - March 25, 2014 Category: Genetics & Stem Cells Authors: Li M, Ling B, Xiao T, Tan J, An N, Han N, Guo S, Cheng S, Zhang K Tags: Gene Source Type: research

Sp1 and Sp3 transcription factors regulate the basal expression of human microsomal epoxide hydrolase (EPHX1) through interaction with the proximal E1b far upstream promoter.
Abstract Microsomal epoxide hydrolase (mEH, EPHX1) is a critical biotransformation enzyme, catalyzing the metabolism of many xenobiotics. Human mEH is transcribed using alternative promoters. The proximal E1 promoter is active in liver while the far upstream E1b promoter drives the expression of mEH in all tissues including liver. Although several liver-specific transcription factors have been identified in the regulation of E1 transcription, little is known regarding the mechanisms of E1b transcriptional regulation. Genome-wide mapping of DNase I hypersensitive sites revealed an open chromatin region between nucl...
Source: Gene - December 3, 2013 Category: Genetics & Stem Cells Authors: Su S, Omiecinski CJ Tags: Gene Source Type: research

Regulation of cell proliferation and migration in gallbladder cancer by zinc finger X-chromosomal protein.
Abstract Gallbladder carcinoma (GBC) is one of the mostly aggressive and fatal malignancies. However, little is known about the oncogenic genes that contributed to the development of GBC. Zinc finger X-chromosomal protein (ZFX) was a novel member of the Krueppel C2H2-type zinc-finger protein family and its down-regulation led to impaired cell growth in human laryngeal squamous cell carcinoma. Here, we aim to investigate the function of ZFX in GBC cell proliferation and migration. Loss of function analysis was performed on GBC cell line (GBC-SD) using lentivirus-mediated siRNA against ZFX. The proliferation, in vit...
Source: Gene - July 13, 2013 Category: Genetics & Stem Cells Authors: Tan Z, Zhang S, Li M, Wu X, Weng H, Ding Q, Cao Y, Bao R, Shu Y, Mu J, Ding Q, Wu W, Yang J, Zhang L, Liu Y Tags: Gene Source Type: research

Characterization of a novel chromodomain-containing gene from the silkworm, Bombyx mori.
Abstract Heterochromatin protein 1 (HP1) is an evolutionarily conserved protein across different eukaryotic species, and is crucial in the establishment and maintenance of heterochromatin. HP1 proteins have two distinct functional domains, an N-terminal chromodomain (CD) and a C-terminal chromoshadow domain (CSD), which are required for the selective binding of HP1 proteins to modified histones. During our screen for HP1-like proteins in the Bombyx mori genome, we found a novel silkworm gene, Bombyx mori chromodomain protein 1 (BmCdp1), encoding a putative chromobox protein with only two CDs. The BmCdp1 family pro...
Source: Gene - July 11, 2013 Category: Genetics & Stem Cells Authors: Shoji K, Kiuchi T, Hara K, Kawamoto M, Kawaoka S, Arimura SI, Tsutsumi N, Sugano S, Suzuki Y, Shimada T, Katsuma S Tags: Gene Source Type: research