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Source: Molecular Carcinogenesis

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Total 68 results found since Jan 2013.

Expression and prognostic role of ubiquitination factor E4B in primary hepatocellular carcinoma
Ubiquitination factor E4B (UBE4B) has been speculated to have contradictory functions upon tumorigenesis as an oncogene or tumor suppressor in different types of cancers. We investigated the expression and prognostic role of UBE4B in primary hepatocellular carcinoma (HCC) using cell lines and 149 archived HCC samples. Correlation between the functions of UBE4B in HCC was also explored. We used human HCC cell lines (HepG2, Hep3B, SK‐Hep1, Huh7, SMMC‐7721, BEL‐7402) and a normal hepatocyte cell line (LO2) along with HCC samples from patients who had undergone resection for HCC previously at our hospital. A battery of m...
Source: Molecular Carcinogenesis - January 3, 2015 Category: Molecular Biology Authors: Xiao‐Fei Zhang, Qiu‐Zhong Pan, Ke Pan, De‐Sheng Weng, Qi‐Jing Wang, Jing‐Jing Zhao, Jia He, Qing Liu, Dan‐Dan Wang, Shan‐Shan Jiang, Hai‐Xia Zheng, Lin Lv, Chang‐Long Chen, Hong‐Xia Zhang, Jian‐Chuan Xia Tags: Research Article Source Type: research

PTN signaling: Components and mechanistic insights in human ovarian cancer
In this study, we show that PTN is expressed, produced, and secreted in a panel of EOC cell lines. PTN levels in serous ovarian tumor tissues are on average 3.5‐fold higher relative to normal tissue and PTN is detectable in serum samples of patients with EOC. PTPRZ1 is also expressed and produced by EOC cells and is found to be up‐regulated in serous ovarian tumor tissue relative to normal ovarian surface epithelial tissue (P < 0.05). Gene silencing of PTPRZ1 in EOC cell lines using siRNA‐mediated knockdown shows that PTPRZ1 is essential for viability and results in significant apoptosis with no effect on the ...
Source: Molecular Carcinogenesis - November 21, 2014 Category: Molecular Biology Authors: Geetika Sethi, Youngjoo Kwon, Rebecca J. Burkhalter, Harsh B. Pathak, Rashna Madan, Sarah McHugh, Safinur Atay, Smruthi Murthy, Ossama W. Tawfik, Andrew K. Godwin Tags: Research Article Source Type: research

Pioglitazone, a PPARγ agonist, inhibits growth and invasion of human hepatocellular carcinoma via blockade of the rage signaling
Pioglitazone (PGZ), a synthetic PPARγ ligand, is known to have anti‐tumor activity. However, it is unclear how it acts against hepatocellular carcinoma (HCC). We hypothesized that the pathological receptor for advanced glycation end products (RAGE) is involved in the PGZ anti‐tumor process. To test this notion, human primary HCC tissues and corresponding adjacent non‐cancerous tissues (ANCT) from 75 consecutive cases were analyzed. The expression and clinical significance of RAGE was assessed by immunohistochemical assay through tissue microarray. After HCC cells were pretreated with different concentrations of PGZ,...
Source: Molecular Carcinogenesis - October 12, 2014 Category: Molecular Biology Authors: Yuan Yang, Ling‐Hao Zhao, Bo Huang, Ruo‐Yu Wang, Sheng‐Xian Yuan, Qi‐Fei Tao, Yong Xu, Han‐Yong Sun, Chuan Lin, Wei‐Ping Zhou Tags: Research Article Source Type: research

Zbed3 contributes to malignant phenotype of lung cancer via regulating β‐catenin and P120‐catenin 1
Our previous studies indicate that abnormal expression of several Wnt signaling molecules including Axin, Dvl and β‐catenin are involved in proliferation, invasion and metastasis of lung cancer. Zbed3 was found to inhibit function of Axin‐GSK3β complex and thus lead to accumulation of β‐catenin in NIH3T3 and HEK293T cells. However its function in malignant tumors is largely unknown. Here we investigate the clinico‐pathological significance of Zbed3 expression and its function in non‐small cell lung cancer. We use immunohistochemistry and Western blotting to examine Zbed3 expression in non‐small cell lung can...
Source: Molecular Carcinogenesis - September 27, 2014 Category: Molecular Biology Authors: Chuifeng Fan, Guiyang Jiang, Xiupeng Zhang, Yuan Miao, Xuyong Lin, Lan Luan, Zhonghai Xu, Yijun Zhang, Huanyu Zhao, Di Liu, Enhua Wang Tags: Research Article Source Type: research

Targeting Fyn in Ras‐transformed cells induces F‐actin to promote adherens junction‐mediated cell–cell adhesion
Fyn, a member of the Src family kinases (SFK), is an oncogene in murine epidermis and is associated with cell–cell adhesion turnover and induction of cell migration. Additionally, Fyn upregulation has been reported in multiple tumor types, including cutaneous squamous cell carcinoma (cSCC). Introduction of active H‐Ras(G12V) into the HaCaT human keratinocyte cell line resulted in upregulation of Fyn mRNA (200‐fold) and protein, while expression of other SFKs remained unaltered. Transduction of active Ras or Fyn was sufficient to induce an epithelial‐to‐mesenchymal transition in HaCaT cells. Inhibition of Fyn acti...
Source: Molecular Carcinogenesis - June 29, 2014 Category: Molecular Biology Authors: Sarah E. Fenton, Kelli A. Hutchens, Mitchell F. Denning Tags: Research Article Source Type: research

Erbb2 up‐regulation of ADAM12 expression accelerates skin cancer progression
Solar ultraviolet (UV) radiation can cause severe damage to the skin and is the primary cause of most skin cancer. UV radiation causes DNA damage leading to mutations and also activates the Erbb2/HER2 receptor through indirect mechanisms involving reactive oxygen species. We hypothesized that Erbb2 activation accelerates the malignant progression of UV‐induced skin cancer. Following the induction of benign squamous papillomas by UV exposure of v‐rasHa transgenic Tg.AC mice, mice were treated topically with the Erbb2 inhibitor AG825 and tumor progression monitored. AG825 treatment reduced tumor volume, increased tumor r...
Source: Molecular Carcinogenesis - May 5, 2014 Category: Molecular Biology Authors: Velidi H. Rao, Kristen Vogel, Jodi K. Yanagida, Nitin Marwaha, Amrit Kandel, Carol Trempus, Susan K. Repertinger, Laura A. Hansen Tags: Research Article Source Type: research

Abrogation of STAT3 signaling cascade by zerumbone inhibits proliferation and induces apoptosis in renal cell carcinoma xenograft mouse model
Persistent activation of signal transducer and activator of transcription 3 (STAT3) is one of the characteristic features of renal cell carcinoma (RCC) and often linked to its deregulated proliferation, survival, and angiogenesis. In the present report, we investigated whether zerumbone, a sesquiterpene, exerts its anticancer effect through modulation of STAT3 activation pathway. The pharmacological effect of zerumbone on STAT3 activation, associated protein kinases and phosphatase, and apoptosis was investigated using both RCC cell lines and xenograft mouse model. We observed that zerumbone suppressed STAT3 activation in ...
Source: Molecular Carcinogenesis - May 3, 2014 Category: Molecular Biology Authors: Muthu K. Shanmugam, Peramaiyan Rajendran, Feng Li, Chulwon Kim, Sakshi Sikka, Kodappully Sivaraman Siveen, Alan Prem Kumar, Kwang Seok Ahn, Gautam Sethi Tags: Research Article Source Type: research

PPARδ deficiency disrupts hypoxia‐mediated tumorigenic potential of colon cancer cells
Peroxisome proliferator‐activated receptor (PPAR) δ is highly expressed in colon epithelial cells and closely linked to colon carcinogenesis. However, the role of PPARδ in colon cancer cells in a hypoxic tumor microenvironment is not fully understood. We found that expression of the tumor‐promoting cytokines, IL‐8 and VEGF, induced by hypoxia (<1% O2) and deferoxamine (a hypoxia mimetic) was significantly attenuated in PPARδ‐deficient HCT116 colon cancer cells. Consequently, PPARδ‐knockout colon cancer cells exposed to hypoxia and deferoxamine failed to stimulate endothelial cell vascularization and macrop...
Source: Molecular Carcinogenesis - March 9, 2014 Category: Molecular Biology Authors: Eunshil Jeong, Jung Eun Koo, Sang Hyeon Yeon, Mi‐Kyoung Kwak, Daniel H. Hwang, Joo Young Lee Tags: Research Article Source Type: research

Src mediates extracellular signal‐regulated kinase 1/2 activation and autophagic cell death induced by cardiac glycosides in human non‐small cell lung cancer cell lines
Aberrant Na+/K+‐ATPases (NKA) expression is closely related to the incidence and development of cancer, making NKA targeted cancer therapy more intriguing. Cardiac glycosides (CGs) belong to NKA inhibitors and possess potent anti‐cancer properties in many cancers. Our previous work demonstrates that CGs family member digoxin or ouabain induces autophagic cell death in human non‐small cell lung cancer (NSCLC) cell lines through regulation of both mammalian target of rapamycin and extracellular signal‐regulated kinase 1/2 (ERK1/2) pathway. However, what acts as an upstream regulator of ERK1/2 activation during autoph...
Source: Molecular Carcinogenesis - March 1, 2014 Category: Molecular Biology Authors: Yan Wang, Yuechen Zhan, Rong Xu, Rongguang Shao, Jiandong Jiang, Zhen Wang Tags: Research Article Source Type: research

Involvement of RECK in gambogic acid induced anti‐invasive effect in A549 human lung carcinoma cells
Gambogic acid (GA), a xanthone derived from the resin of the Garcinia hanburyi, has been demonstrated possessing anti‐metastatic activity in vitro and in vivo. Reversion‐inducing cysteine‐rich protein with Kazal motifs (RECK), a membrane‐anchored glycoprotein negatively regulating matrix metalloproteinases (MMPs), plays an important role in tumor invasion and metastasis. The present study investigates the regulatory effect of GA on RECK expression and the role of RECK in GA‐induced anti‐invasion in A549 human lung cancer cells. Our results showed that GA dose‐dependently inhibited cell invasion and suppressed...
Source: Molecular Carcinogenesis - February 1, 2014 Category: Molecular Biology Authors: Qi Qi, Na Lu, Chenglin Li, Jie Zhao, Wei Liu, Qidong You, Qinglong Guo Tags: Research Article Source Type: research

Regulation of HIF‐1 alpha by the proprotein convertases furin and PC7 in human squamous carcinoma cells
Proprotein convertases (PC), a family of serine proteases, process cancer‐related substrates such as growth factors, growth factor receptors, cell adhesion molecules, metalloproteinases, etc. HIF‐1α is a major transcription factor involved in tumorigenesis by sensing intratumoral hypoxia. Furin (PCSK3) is one of the numerous target genes regulated by HIF‐1α transactivation and its distribution into endosomal compartments and onto the cell surface can be triggered by hypoxia via HIF‐1α. siRNAs to knockdown PCs were transfected into cells alone or in combination with different drug treatments. Protein and RNA expr...
Source: Molecular Carcinogenesis - January 1, 2014 Category: Molecular Biology Authors: Jian Fu, Jirong Zhang, Yulan Gong, Courtney Lyons Testa, Andres J. Klein‐Szanto Tags: Research Article Source Type: research

Expression of far upstream element (FUSE) binding protein 1 in human glioma is correlated with c‐Myc and cell proliferation
Abstract Glioma is one of the most common type of primary intracranial tumor. Although great advances have been achieved in treatment of glioma, the underlying molecular mechanisms remain largely unknown. Previous studies demonstrated that FBP1 is a transcriptional regulator of c‐Myc and acts as an important prognostic indicator in many cancers. Our study aimed to assess the expression and function of FBP1 in human glioma. Immunohistochemical and Western blot analysis were performed in human glioma and normal brain tissues. High FBP1 expression (located in cell nuclei) was observed in 70 samples and its level was correla...
Source: Molecular Carcinogenesis - December 17, 2013 Category: Molecular Biology Authors: Zongmei Ding, Xiancheng Liu, Yonghua Liu, Jianguo Zhang, Xianting Huang, Xiaojing Yang, Li Yao, Gang Cui, Donglin Wang Tags: Research Article Source Type: research

Phenotypic impact of deregulated expression of class I histone deacetylases in urothelial cell carcinoma of the bladder
ABSTRACT Deregulated expression of histone deacetylases (HDACs) has been implicated in tumorigenesis. Herein, we investigated class I HDACs expression in bladder urothelial cell carcinoma (BUCC), its prognostic value and biological significance. Significantly increased transcript levels of all HDACs were found in BUCC compared to 20 normal mucosas, and these were higher in lower grade and stage tumors. Increased HDAC3 levels were associated with improved patient survival. SiRNA experiments showed decrease cell viability and motility, and increased apoptosis. We concluded that class I HDACs play an important role in bladder...
Source: Molecular Carcinogenesis - November 30, 2013 Category: Molecular Biology Authors: Susana Junqueira‐Neto, Filipa Q. Vieira, Diana Montezuma, Natália R. Costa, Luís Antunes, Tiago Baptista, Ana Isabel Oliveira, Inês Graça, Ângelo Rodrigues, José S. Magalhães, Jorge Oliveira, Rui Henrique, Carmen Jerónimo Tags: Research Article Source Type: research

Silymarin suppresses the PGE2‐induced cell migration through inhibition of EP2 activation; G protein‐dependent PKA‐CREB and G protein‐independent Src‐STAT3 signal pathways
Abstract Silymarin has been known as a chemopreventive agent, and possesses multiple anti‐cancer activities including induction of apoptosis, inhibition of proliferation and growth, and blockade of migration and invasion. However, whether silymarin could inhibit prostaglandin (PG) E2‐induced renal cell carcinoma (RCC) migration and what are the underlying mechanisms are not well elucidated. Here, we found that silymarin markedly inhibited PGE2‐stimulated migration. PGE2 induced G protein‐dependent CREB phosphorylation via protein kinase A (PKA) signaling, and PKA inhibitor (H89) inhibited PGE2‐mediated migration....
Source: Molecular Carcinogenesis - October 1, 2013 Category: Molecular Biology Authors: Seon Min Woo, Kyoung‐Jin Min, In Gyeong Chae, Kyung‐Soo Chun, Taeg Kyu Kwon Tags: Research Article Source Type: research

MicroRNA‐106b modulates epithelial–mesenchymal transition by targeting TWIST1 in invasive endometrial cancer cell lines
In this study, we sought to explore the role of TWIST1 in inducing EMT in representative type II EC cell lines, and to determine the miRNAs involved in regulating TWIST1 gene expression. Functional analysis suggested that TWIST1 contributes to the EMT phenotypes of EC cells, as evidenced by the acquisition of fibroblast‐like properties, enhanced invasiveness, and induction of an EN‐switch (downregulation of epithelial marker E‐cadherin and upregulation of mesenchymal marker N‐cadherin). Conversely, silencing of TWIST1 by siRNA inhibited cell invasion and the mesenchymal phenotype, which was accompanied by a reversi...
Source: Molecular Carcinogenesis - September 3, 2013 Category: Molecular Biology Authors: Peixin Dong, Masanori Kaneuchi, Hidemichi Watari, Satoko Sudo, Noriaki Sakuragi Tags: Research Article Source Type: research