• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

Molecular cloning, prokaryotic expression and the anti-inflammatory activity of porcine PRDX5
In this study, the full-length cDNA encoding porcine PRDX5 (pPRDX5) was cloned. Subsequently, using porcine alveolar macrophages (PAMs), the target cells of PRRSV infection in vivo, we found that the recombinant pPRDX5 protein inhibited inflammatory responses induced by tumor necrosis factor alpha (TNF-α) or porcine reproductive and respiratory syndrome virus (PRRSV), a virus causing severe interstitial pneumonia in pigs. By contrast, knockdown of endogenous pPRDX5 with specific siRNA enhanced inflammatory responses induced by TNF-α or PRRSV. We also demonstrated that the involvement of pPRDX5 in inflammation regulation ...
Source: Developmental and Comparative Immunology - August 19, 2022 Category: Allergy & Immunology Authors: Panpan Ma Yanrong Zhou Puxian Fang Wenting Ke Shaobo Xiao Liurong Fang Source Type: research

CYP1A1 mediates the suppression of major inflammatory cytokines in pulmonary macrophage (PAM) cell lines caused by mycoplasma hyponeumoniae.
In this study, pulmonary alveolar macrophage (PAM) cell lines with CYP1A1 overexpression or siRNA-mediated CYP1A1 silencing were used to explore the biological function and regulatory mechanism of CYP1A1 gene expression changed on the inflammatory response of pigs infected with M. hyopneumoniae. The results showed that the cells overexpressing CYP1A1 infected with M. hyopneumoniae led to a rapid increase in PPAR-γ expression, which resulted in decreasing the levels of several inflammatory cytokines including IL-1β, IL-6, IL-8 and TNF-α. On the contrary, this effect was just opposite in CYP1A1-RNAi cells infected with M....
Source: Developmental and Comparative Immunology - June 30, 2016 Category: Allergy & Immunology Authors: Fang X, Zhao W, Xu J, Tu F, Wang X, Li B, Fu Y, Ren S Tags: Dev Comp Immunol Source Type: research