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Gene therapy in the anterior eye segment
Curr Gene Ther. 2021 Apr 22. doi: 10.2174/1566523221666210423084233. Online ahead of print.ABSTRACTThis review provides comprehensive information about the advances in gene therapy in the anterior segment of the eye including cornea, conjunctiva, lacrimal gland, and trabecular meshwork. We discuss gene delivery systems including viral and non-viral vectors as well as gene editing techniques, mainly CRISPR-Cas9, and epigenetic treatments including antisense and siRNA therapeutics. We also provide a detailed analysis of various anterior segment diseases where gene therapy has been tested with corresponding outcomes. Disease ...
Source: Current Gene Therapy - April 27, 2021 Category: Genetics & Stem Cells Authors: Cynthia Amador Ruchi Shah Sean Ghiam Andrei A Kramerov Alexander V Ljubimov Source Type: research

GSE113008 mRNA sequencing of Aniridia limbal epithelial cells.
Contributors : Karl Nordstr öm ; Jasmin KirchSeries Type : Expression profiling by high throughput sequencingOrganism :PAX6-related Aniridia is a sight-threatening disease due to progression of secondary glaucoma and aniridia associated keratopathy (AAK). Changes or loss of limbal epithelial progenitors causes the epithelial surfaces defects. We analyzed how PAX6 contribute to this with a two-step approach. 1) mRNA Sequencing of limbal epithelial cells isolated from controls and aniridia patients. 2) confirming the bioinformatical and literature-based result list on a siRNA based primary aniridia cell model (PAX6- knockdo...
Source: GEO: Gene Expression Omnibus - October 24, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Source Type: research

LTBP2 knockdown and oxidative stress affect glaucoma features including TGF β pathways, ECM genes expression and apoptosis in trabecular meshwork cells.
LTBP2 knockdown and oxidative stress affect glaucoma features including TGFβ pathways, ECM genes expression and apoptosis in trabecular meshwork cells. Gene. 2018 Jun 13;: Authors: Suri F, Yazdani S, Elahi E Abstract Glaucoma is the leading cause of irreversible blindness worldwide. Although the etiology of glaucoma is incompletely understood, it is known that the extracellular matrix (ECM) of the trabecular meshwork, oxidative stress, TGFβ signaling pathways, and apoptosis are important components of glaucoma pathogenesis. These components appear to be interrelated, but knowledge on their interacti...
Source: Gene - June 13, 2018 Category: Genetics & Stem Cells Authors: Suri F, Yazdani S, Elahi E Tags: Gene Source Type: research

Latent TGF-{beta} binding protein-2 is essential for the development of ciliary zonule microfibrils
In this study, we explore the in vivo function of LTBP-2 by generating Ltbp2–/– mice. Ltbp2–/– mice survived to adulthood but developed lens luxation caused by compromised ciliary zonule formation without a typical phenotype related to glaucoma, suggesting that LTBP-2 deficiency primarily causes lens dislocation but not glaucoma. The suppression of LTBP2 expression in cultured human ciliary epithelial cells by siRNA disrupted the formation of the microfibril meshwork by the cells. Supplementation of recombinant LTBP-2 in culture medium not only rescued the microfibril meshwork formation in LTBP2-sup...
Source: Human Molecular Genetics - October 8, 2014 Category: Genetics & Stem Cells Authors: Inoue, T., Ohbayashi, T., Fujikawa, Y., Yoshida, H., Akama, T. O., Noda, K., Horiguchi, M., Kameyama, K., Hata, Y., Takahashi, K., Kusumoto, K., Nakamura, T. Tags: ARTICLES Source Type: research