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Cancer: Prostate Cancer

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Total 17 results found since Jan 2013.

IGT mediated Nanog siRNA delivery in prostate cancer cells improves chemosensitization of Epirubicin in vitro
In this report, we have successfully delivered siRNA against Nanog with the help of pentafluorobenzyl modified Internal Oligo-guanidinium transporter (IGT) that has previously shown promising results in peptide and antisense morpholino delivery. Nanog downregulation in prostate cancer cell line DU145 in serum containing media led to suppression of associated proteins such as KLF4, FAK and cMyc and also enhanced the chemosensitivity of Epirubicin, an anthracycline based drug, in DU145 cells by associated MDR-1 downregulation in vitro. These results show that IGT is a promising candidate for siRNA delivery and its conjugatio...
Source: Bioorganic and Medicinal Chemistry Letters - October 9, 2022 Category: Chemistry Authors: Shalini Gupta Ujjal Das Surajit Sinha Source Type: research

Multi-targeting siRNA nanoparticles for simultaneous inhibition of PI3K and Rac1 in PTEN-deficient prostate cancer
Publication date: Available online 16 April 2021Source: Journal of Industrial and Engineering ChemistryAuthor(s): Min Ju Kim, Hyosuk Kim, Xueliang Gao, Ju Hee Ryu, Yoosoo Yang, Ick Chan Kwon, Thomas M. Roberts, Sun Hwa Kim
Source: Journal of Industrial and Engineering Chemistry - April 17, 2021 Category: Chemistry Source Type: research

Molecules, Vol. 25, Pages 1320: Evodiamine Mitigates Cellular Growth and Promotes Apoptosis by Targeting the c-Met Pathway in Prostate Cancer Cells
Blough Ahn Evodiamine (EVO) is an indoloquinazoline alkaloid that exerts its various anti-oncogenic actions by blocking phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt), mitogen-activated protein kinase (MAPK), c-Met, and nuclear factor kappa B (NF-B) signaling pathways, thus leading to apoptosis of tumor cells. We investigated the ability of EVO to affect hepatocyte growth factor (HGF)-induced c-Met/Src/STAT3 activation cascades in castration-resistant prostate cancer (CRPC). First, we noted that EVO showed cytotoxicity and anti-proliferation activities in PC-3 and DU145 cells. Next, we found that EVO m...
Source: Molecules - March 12, 2020 Category: Chemistry Authors: Hwang Um Chinnathambi Alharbi Narula Namjoshi Blough Ahn Tags: Article Source Type: research

Molecules, Vol. 25, Pages 1102: Ginseng Gintonin Contains Ligands for GPR40 and GPR55
ng Seung-Yeol Nah Gintonin, a novel ginseng-derived glycolipoprotein complex, has an exogenous ligand for lysophosphatidic acid (LPA) receptors. However, recent lipid analysis of gintonin has shown that gintonin also contains other bioactive lipids besides LPAs, including linoleic acid and lysophosphatidylinositol (LPI). Linoleic acid, a free fatty acid, and LPI are known as ligands for the G-protein coupled receptors (GPCR), GPR40, and GPR55, respectively. We, herein, investigated whether gintonin could serve as a ligand for GPR40 and GPR55, using the insulin-secreting beta cell-derived cell line INS-1 and the human...
Source: Molecules - March 1, 2020 Category: Chemistry Authors: Yeon-Jin Cho Sun-Hye Choi Rami Lee Hongik Hwang Hyewhon Rhim Ik-Hyun Cho Hyoung-Chun Kim Jeong-Ik Lee Sung-Hee Hwang Seung-Yeol Nah Tags: Article Source Type: research

Phosphorylation of HSP90 by protein kinase A is essential for the nuclear translocation of androgen receptor Signal Transduction
The androgen receptor (AR) is often activated in prostate cancer patients undergoing androgen-ablative therapy because of the activation of cellular pathways that stimulate the AR despite low androgen levels. In many of these tumors, the cAMP-dependent protein kinase A (PKA) pathway is activated. Previous studies have shown that PKA can synergize with low levels of androgen to enhance androgen signaling and consequent cell proliferation, leading to castration-resistant prostate cancer. However, the mechanism by which PKA causes AR stimulation in the presence of low/no androgen is not established yet. Here, using immunofluo...
Source: Journal of Biological Chemistry - May 30, 2019 Category: Chemistry Authors: Manisha Dagar, Julie Pratibha Singh, Gunjan Dagar, Rakesh K. Tyagi, Gargi Bagchi Tags: Editors ' Picks Source Type: research

Molecules, Vol. 22, Pages 612: c-Jun Contributes to Transcriptional Control of GNA12 Expression in Prostate Cancer Cells
In this study, we explored the control of expr ession of GNA12 in prostate cancer cells. Initial studies on LnCAP (low metastatic potential, containing low levels of GNA12) and PC3 (high metastatic potential, containing high GNA12 levels) cells revealed that GNA12 mRNA levels correlated with protein levels, suggesting control at the transcriptio nal level. To identify potential factors controlling GNA12 transcription, we cloned the upstream 5′ regulatory region of the human GNA12 gene and examined its activity using reporter assays. Deletion analysis revealed the highest level of promoter activity in a 784 bp region, and...
Source: Molecules - April 10, 2017 Category: Chemistry Authors: Udhaya Udayappan Patrick Casey Tags: Article Source Type: research

JunD in TGF-{beta} Signaling in Prostate Cancer Cells Signal Transduction
In conclusion, we show that specific Jun family members exert differential effects on proliferation in prostate cancer cells in response to TGF-β, and inhibition of cell proliferation by TGF-β requires degradation of JunD protein.
Source: Journal of Biological Chemistry - August 18, 2016 Category: Chemistry Authors: Millena, A. C., Vo, B. T., Khan, S. A. Tags: Cell Biology Source Type: research

Androgen Receptor in Telomeres DNA and Chromosomes
Androgen receptor (AR) plays a role in maintaining telomere stability in prostate cancer cells, as AR inactivation induces telomere dysfunction within 3 h. Since telomere dysfunction in other systems is known to activate ATM (ataxia telangiectasia mutated)-mediated DNA damage response (DDR) signaling pathways, we investigated the role of ATM-mediated DDR signaling in AR-inactivated prostate cancer cells. Indeed, the induction of telomere dysfunction in cells treated with AR-antagonists (Casodex or MDV3100) or AR-siRNA was associated with a dramatic increase in phosphorylation (activation) of ATM and its downstream effector...
Source: Journal of Biological Chemistry - October 16, 2015 Category: Chemistry Authors: Reddy, V., Wu, M., Ciavattone, N., McKenty, N., Menon, M., Barrack, E. R., Reddy, G. P.-V., Kim, S.-H. Tags: Cell Biology Source Type: research

AMPK in TRAIL Combination-induced Apoptosis Signal Transduction
Prostate cancer (PCa) is one of the most frequently diagnosed cancers in men with limited treatment options for the hormone-resistant forms. Development of novel therapeutic options is critically needed to target advanced forms. Here we demonstrate that combinatorial treatment with the thiazolidinedione troglitazone (TZD) and TNF-related apoptosis-inducing ligand (TRAIL) can induce significant apoptosis in various PCa cells independent of androgen receptor status. Because TZD is known to activate AMP-activated protein kinase (AMPK), we determined whether AMPK is a molecular target mediating this apoptotic cascade by utiliz...
Source: Journal of Biological Chemistry - September 4, 2015 Category: Chemistry Authors: Santha, S., Viswakarma, N., Das, S., Rana, A., Rana, B. Tags: Cell Biology Source Type: research

REPORT: Exosome-mediated Transfer of the {alpha}v{beta}6 Integrin Signal Transduction
In this study, we have investigated whether transfer of integrins through exosomes between prostate cancer (PrCa) cells occurs and whether transferred integrins promote cell adhesion and migration. Among others, we have focused on the αvβ6 integrin, which is not detectable in normal human prostate but is highly expressed in human primary PrCa as well as murine PrCa in Ptenpc−/− mice. After confirming the fidelity of the exosome preparations by electron microscopy, density gradient, and immunoblotting, we determined that the αvβ6 integrin is actively packaged into exosomes isolated from PC3 and RWPE PrCa cell lines....
Source: Journal of Biological Chemistry - February 20, 2015 Category: Chemistry Authors: Fedele, C., Singh, A., Zerlanko, B. J., Iozzo, R. V., Languino, L. R. Tags: Reports Source Type: research

Usp12 Is a Novel Positive Regulator of the AR Protein Synthesis and Degradation
In this report we demonstrate that Usp12, in complex with Uaf-1 and WDR20, deubiquitinates the AR to enhance receptor stability and transcriptional activity. Our data show that Usp12 acts in a pro-proliferative manner by stabilizing AR and enhancing its cellular function.
Source: Journal of Biological Chemistry - November 8, 2013 Category: Chemistry Authors: Burska, U. L., Harle, V. J., Coffey, K., Darby, S., Ramsey, H., O'Neill, D., Logan, I. R., Gaughan, L., Robson, C. N. Tags: Molecular Bases of Disease Source Type: research

Ion exchange liquid chromatography method for the direct determination of small ribonucleic acids.
We present here a one step sample preparation combined with non-denaturing anion exchange chromatography with UV detection for the quantitation of siRNA and its chain shortened metabolites. The sample preparation uses a novel lysis buffer with proteinase K to effectively isolate siRNA from cells and formulated media with greater than 95% recovery. The ion exchange chromatography allows for a lower limit of quantitation of 6ngmL(-1) in cells and media equivalent to 6ng/200,000cells. This method is applied to study the uptake of siRNA in prostate cancer cells and the disappearance in the media and siRNA metabolism. siRNA met...
Source: Analytica Chimica Acta - October 7, 2013 Category: Chemistry Authors: McGinnis AC, Cummings BS, Bartlett MG Tags: Anal Chim Acta Source Type: research

TR4 Promotes Chemoresistance in PCa Stem Cells Gene Regulation
Prostate cancer (PCa) stem/progenitor cells are known to have higher chemoresistance than non-stem/progenitor cells, but the underlying molecular mechanism remains unclear. We found the expression of testicular nuclear receptor 4 (TR4) is significantly higher in PCa CD133+ stem/progenitor cells compared with CD133− non-stem/progenitor cells. Knockdown of TR4 levels in the established PCa stem/progenitor cells and the CD133+ population of the C4-2 PCa cell line with lentiviral TR4 siRNA led to increased drug sensitivity to the two commonly used chemotherapeutic drugs, docetaxel and etoposide, judging from significantly re...
Source: Journal of Biological Chemistry - June 7, 2013 Category: Chemistry Authors: Yang, D.-R., Ding, X.-F., Luo, J., Shan, Y.-X., Wang, R., Lin, S.-J., Li, G., Huang, C.-K., Zhu, J., Chen, Y., Lee, S. O., Chang, C. Tags: Cell Biology Source Type: research

E-cadherin Expression Requires SPDEF Gene Regulation
Loss of E-cadherin is one of the key steps in tumor progression. Our previous studies demonstrate that SAM pointed domain-containing ETS transcription factor (SPDEF) inhibited prostate cancer metastasis in vitro and in vivo. In the present study, we evaluated the relationship between SPDEF and E-cadherin expression in an effort to better understand the mechanism of action of SPDEF in prostate tumor cell invasion and metastasis. The results presented here demonstrate a direct correlation between expression of E-cadherin and SPDEF in prostate cancer cells. Additional data demonstrate that modulation of E-cadherin and SPDEF h...
Source: Journal of Biological Chemistry - April 26, 2013 Category: Chemistry Authors: Pal, M., Koul, S., Koul, H. K. Tags: Molecular Bases of Disease Source Type: research