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Genes, Vol. 12, Pages 1373: High Frequency of Tumor Propagating Cells in Fusion-Positive Rhabdomyosarcoma
In this study we investigated the expression of key stem cell markers, developed a sphere assay, and investigated the seven most common FPRMS cell lines for subpopulations of tumor propagating cancer stem-like cells, also called cancer stem cells (CSCs). Moreover, loss- and gain-of-functions of the stem cell genes SOX2, OCT4, and NANOG were investigated in the same cells. Single-cell clonal analysis was performed in vitro as well as in vivo. We found that no stable CSC subpopulation could be enriched in FPRMS. Unlike depletion of PAX3-FOXO1, neither overexpression nor siRNA-mediated downregulation of SOX2, OCT4, and NANOG ...
Source: Genes - August 31, 2021 Category: Genetics & Stem Cells Authors: Melanie Generali Sampoorna Satheesha Peter K. Bode Debora Wanner Beat W. Sch äfer Elisa A. Casanova Tags: Article Source Type: research

GSE140040 Next-generation sequencing reveals a novel role of lysine-specific demethylase 1 in adhesion of rhabdomyosarcoma cells RNA-seq
This study aims at elucidating changes in the RNA expression profile by RNA Sequencing in control and LSD1 knockdown conditions using transient silencing with siRNA. In addition, ChIP Sequencing was employed to investigate changes in the histone methylation pattern of H3K4me2 as a target of LSD1 in control and LSD1 knockdown conditions.
Source: GEO: Gene Expression Omnibus - November 7, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE140039 Next-generation sequencing reveals a novel role of lysine-specific demethylase 1 in adhesion of rhabdomyosarcoma cells ChIP-seq
This study aims at elucidating changes in the RNA expression profile by RNA Sequencing in control and LSD1 knockdown conditions using transient silencing with siRNA. In addition, ChIP Sequencing was employed to investigate changes in the histone methylation pattern of H3K4me2 as a target of LSD1 in control and LSD1 knockdown conditions.
Source: GEO: Gene Expression Omnibus - November 7, 2019 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Homo sapiens Source Type: research

DUX4-induced dsRNA and MYC mRNA stabilization activate apoptotic pathways in human cell models of facioscapulohumeral dystrophy
by Sean C. Shadle, Jun Wen Zhong, Amy E. Campbell, Melissa L. Conerly, Sujatha Jagannathan, Chao-Jen Wong, Timothy D. Morello, Silv ère M. van der Maarel, Stephen J. Tapscott Facioscapulohumeral dystrophy (FSHD) is caused by the mis-expression of DUX4 in skeletal muscle cells. DUX4 is a transcription factor that activates genes normally associated with stem cell biology and its mis-expression in FSHD cells results in apoptosis. To identify genes and pathways necessary for DUX4-mediated apoptosis, we performed an siRNA screen in an RD rhabdomyosarcoma cell line with an inducibleDUX4 transgene. Our screen identified compon...
Source: PLoS Genetics - March 7, 2017 Category: Genetics & Stem Cells Authors: Sean C. Shadle Source Type: research

GSE87495 DUX4-induced dsRNA and MYC mRNA Stabilization Lead to Apoptosis in Human Cell Models of Facioscapulohumeral Dystrophy
Contributors : Sean C Shadle ; Jun W Zhong ; Amy E Campbell ; Melissa L Conerly ; Sujatha Jagannathan ; Chao-Jen Wong ; Timothy D Morello ; Stephen J TapscottSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensFacioscapulohumeral muscular dystophy (FSHD) is caused by the mis-expression of DUX4 in skeletal muscle cells. DUX4 is a transcription factor that activates genes normally associated with stem cell biology and its mis-expression in FSHD cells results in apoptosis. To identify genes and pathways necessary for DUX4-mediated apoptosis, we performed an siRNA screen in an RD rhabdomyosar...
Source: GEO: Gene Expression Omnibus - December 31, 2016 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research