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Specialty: Genetics & Stem Cells
Cancer: Bone Cancers
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Total 18 results found since Jan 2013.
Prognosis-related VDAC1 regulates the proliferation and apoptosis of osteosarcoma cells via the MAPK signaling pathway
In conclusion, prognosis-related VDAC1 can affect osteosarcoma cells' proliferative activity and apoptosis level. The MAPK signaling pathway mediates VDAC1 regulation of osteosarcoma cell development.PMID:36871636 | DOI:10.1016/j.ygeno.2023.110595
Source: Genomics - March 5, 2023 Category: Genetics & Stem Cells Authors: Xu Cai Shaihong Zhu Source Type: research
GSE154529 Identification of ALKBH5 targets in osteosarcoma by methyl RNA immunoprecipitation MeRIP-seq
Contributors : Pooja Yadav ; Manjeet Rao ; Yidong Chen ; Zhao LaiSeries Type : OtherOrganism : Homo sapiensTo identify potential target mRNAs that are demethylated by ALKBH5, meRIP-seq with a m6A antibody was conducted in 143B osteosarcoma cells transfected with scrambled or ALKBH5-siRNA.
Source: GEO: Gene Expression Omnibus - June 28, 2022 Category: Genetics & Stem Cells Tags: Other Homo sapiens Source Type: research
MYC overexpression leads to increased chromatin interactions at super-enhancers and MYC binding sites RESEARCH
The MYC oncogene encodes for the MYC protein and is frequently dysregulated across multiple cancer cell types, making it an attractive target for cancer therapy. MYC overexpression leads to MYC binding at active enhancers, resulting in a global transcriptional amplification of active genes. Because super-enhancers are frequently dysregulated in cancer, we hypothesized that MYC preferentially invades into super-enhancers and alters the cancer genome organization. To that end, we performed ChIP-seq, RNA-seq, circular chromosome conformation capture (4C-seq), and Spike-in Quantitative Hi-C (SIQHiC) on the U2OS osteosarcoma ce...
Source: Genome Research - April 8, 2022 Category: Genetics & Stem Cells Authors: See, Y. X., Chen, K., Fullwood, M. J. Tags: RESEARCH Source Type: research
MYC overexpression leads to increased chromatin interactions at superenhancers and MYC binding sites RESEARCH
The MYC oncogene encodes for the MYC protein and is frequently dysregulated across multiple cancer cell types, making it an attractive target for cancer therapy. MYC overexpression leads to MYC binding at active enhancers, resulting in a global transcriptional amplification of active genes. Since superenhancers are frequently dysregulated in cancer, we hypothesized that MYC preferentially invades into superenhancers and alters the cancer genome organization. To that end, we performed ChIP-seq, RNA-seq, 4C-seq and SIQHiC (Spike-in Quantitative Hi-C) on the U2OS osteosarcoma cell line with tetracycline-inducible MYC. MYC ove...
Source: Genome Research - February 3, 2022 Category: Genetics & Stem Cells Authors: See, Y. X., Chen, K., Fullwood, M. J. Tags: RESEARCH Source Type: research
GSE169322 Serine-Threonine Kinase Receptor-Associated Protein (STRAP) Knockout Decreases the Malignant Phenotype in Neuroblastoma
Conclusion: These novel findings demonstrated that STRAP plays a role in tumorigenesis and maintenance of neuroblastoma stemness.
Source: GEO: Gene Expression Omnibus - July 14, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE173313 Histone H4 lysine 20 mono-methylation directly facilitates chromatin ‘openness’ and promotes transcription of housekeeping genes (ATAC-seq_DRB)
Contributors : Muhammad Shoaib ; Qinming Chen ; Xiangyan Shi ; Nidhi Nair ; Chinmayi Prasanna ; Renliang Yang ; David Walter ; Klaus S Frederiksen ; Einarsson Hjorleifur ; Peter Svenson ; Chuan-Fa Liu ; Karl Ekwall ; Mads Lerdrup ; Lars Nordenski öld ; Claus S SørensenSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensTo study the role of Histone H4 lysine 20 mono- and tri-methylation (H4K20me1, H4K20me3) in relationship to chromatin accessibility and gene expression, we report the application of high-throughput profiling of these marks in synchronized and asynchronous Hu...
Source: GEO: Gene Expression Omnibus - June 4, 2021 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Homo sapiens Source Type: research
GSE156216 Histone H4 lysine 20 mono-methylation directly facilitates chromatin ‘openness’ and promotes transcription of housekeeping genes (ChIP-seq)
Contributors : Muhammad Shoaib ; Qinming Chen ; Xiangyan Shi ; Nidhi Nair ; Chinmayi Prasanna ; Renliang Yang ; Chuan F Liu ; David Walter ; Klaus S Frederiksen ; Peter Svenson ; Karl Ekwall ; Mads Lerdrup ; Lars Nordenski öld ; Claus S SørensenSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiens ; Mus musculusTo study the role of Histone H4 lysine 20 mono- and tri-methylation (H4K20me1, H4K20me3) in relationship to chromatin accessibility and gene expression, we report the application of high-throughput profiling of these marks in Human Osteosarcoma U2OS and Mouse Fibrobl...
Source: GEO: Gene Expression Omnibus - June 4, 2021 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Homo sapiens Mus musculus Source Type: research
GSE156215 Histone H4 lysine 20 mono-methylation directly facilitates chromatin ‘openness’ and promotes transcription of housekeeping genes (ATAC-seq)
Contributors : Muhammad Shoaib ; Qinming Chen ; Xiangyan Shi ; Nidhi Nair ; Chinmayi Prasanna ; Renliang Yang ; Chuan F Liu ; David Walter ; Klaus S Frederiksen ; Peter Svenson ; Karl Ekwall ; Mads Lerdrup ; Lars Nordenski öld ; Claus S SørensenSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiens ; Mus musculusTo study the role of Histone H4 lysine 20 mono- and tri-methylation (H4K20me1, H4K20me3) in relationship to chromatin accessibility and gene expression, we report the application of high-throughput profiling of these marks in synchronized and asynchronous Human Osteo...
Source: GEO: Gene Expression Omnibus - June 4, 2021 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Homo sapiens Mus musculus Source Type: research
Fusion protein EWS-FLI1 is incorporated into a protein granule in cells
We examined EWSR1 and EWS-FLI1 activity in Ewing sarcoma cells by siRNA-mediated knockdown and RNA-seq analysis. More transcripts were affected by the EWSR1 knockdown than expected and these included many EWS-FLI1 regulated genes. We reevaluated physical interactions between EWS-FLI1, EWSR1, and RNA Pol II, and employed a cross-linking based strategy to investigate protein assemblies associated with the proteins. The LC domain of EWS-FLI1 was required for the assemblies observed to form in cells. These results offer new insights into a protein assembly that may enable EWS-FLI1 to bind its wide network of protein partners a...
Source: RNA - May 26, 2021 Category: Genetics & Stem Cells Authors: Nasiha S Ahmed Lucas M Harrell Daniel R Wieland Michelle A Lay Valery F Thompson Jacob C Schwartz Source Type: research
GSE120512 Transcriptome modulation by loss-of-YAP1/TAZ in SK-N-MC Ewing Sarcoma cells
Contributors : Juan Diaz-Martin ; Laura Romero-PerezSeries Type : Expression profiling by arrayOrganism : Homo sapiensYAP1 (Yes-associated protein 1) and TAZ (transcriptional coactivator with PDZ-binding motif, or WWTR1) are nucleo-cytoplasmic shuttling proteins that can function in the nucleus as transcriptional coactivators. Here we sought to evaluate which genes are regulated by endogenous levels of YAP/TAZ. We compared SK-N-MC cells transfected with a control non-targeting siRNA with cells transfected with a mix of siRNA targeting both YAP and TAZ.
Source: GEO: Gene Expression Omnibus - September 27, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
GSE73092 High-throughput RNAi cell viability screen to identify selective targets for EWS-FLI1 positive Ewing sarcoma
Contributors : Kristiina Iljin ; Tao HeSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe have performed a high-throughput RNA interference screen to identify targets inhibiting EWS-FLI1 driven cell proliferation in Ewing sarcoma cells. EWS-FLI1 expressing A673 Ewing sarcoma cells were screened both in presence and absence of EWS-FLI1 shRNA induction with druggable siRNA library. Leucine rich repeats and WD repeat Domain containing 1 (LRWD1) targeting siRNA pool was the strongest anti-proliferative hit identified only in presence of EWS-FLI1. Validation experiments confirmed the anti-...
Source: GEO: Gene Expression Omnibus - September 16, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
