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Budding uninhibited by benzimidazoles-1 (BUB1) regulates EGFR signaling by reducing EGFR internalization
Aging (Albany NY). 2023 Jul 3;15. doi: 10.18632/aging.204820. Online ahead of print.ABSTRACTEGFR signaling initiates upon ligand binding which leads to activation and internalization of the receptor-ligand complex. Here, we evaluated if BUB1 impacted EGFR signaling by regulating EGFR receptor internalization and activation. BUB1 was ablated genomically (siRNA) or biochemically (2OH-BNPP1) in cells. EGF ligand was used to initiate EGFR signaling while disuccinimidyl suberate (DSS) was used for cross linking cellular proteins. EGFR signaling was measured by western immunoblotting and receptor internalization was evaluated by...
Source: Aging - July 3, 2023 Category: Biomedical Science Authors: Shyam Nyati Grant Young Corey Speers Mukesh K Nyati Alnawaz Rehemtulla Source Type: research

HER2 decreases drug sensitivity of ovarian cancer cells via inducing stem cell-like property in a NF κB-dependent way.
In this study, we explored the effect of HER2 on cancer stem cells induction and drug sensitivity of ovarian cancer cell lines. Firstly, we found that HER2 overexpression (HER2 OE) induced, while HER2 knockdown (HER2 KD) decreased CD44+/CD24- population. Consistently, HER2 expression was closely correlated with the sphere formation efficiency (SFE) of ovarian cancer cells. Secondly, we found that NFκB inhibition by specific inhibitor JSH23 or siRNA targeting subunit p65 dramatically impaired the induction of ovarian cancer stem cells by HER2, indicating that NFκB mediated HER2-induced ovarian cancer stem cells. Thirdly, ...
Source: Bioscience Reports - October 12, 2018 Category: Biomedical Science Authors: Wang W, Gao Y, Hai J, Yang J, Duan S Tags: Biosci Rep Source Type: research