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Infectious Disease: SARS
Drug: Polyethylene Glycol
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Total 108 results found since Jan 2013.
Comparison of ordinary reverse transcription real-time polymerase chain reaction (qRT-PCR) with a newly developed one-step single-tube nested real-time RT-PCR (OSN-qRT-PCR) for sensitive detection of SARS-CoV-2 in wastewater
In this study, we attempted to increase the sensitivity of qRT-PCR by developing a one-step single-tube nested qRT-PCR assay (OSN-qRT-PCR). Two variants were developed, oriented to nucleocapsid phosphoprotein gene (N) and to spike protein gene (S), respectively. The performance of conventional qRT-PCR assays oriented to these genes with two novel OSN-qRT-PCR assays were firstly optimized using wastewater artificially contaminated with two encapsidated RNA mimic systems harboring a portion either N or S gene (ENRM and ESRM, respectively). The assays were coupled to a polyethylene glycol-based RNA precipitation/extraction me...
Source: Environmental Science and Pollution Research International - August 8, 2023 Category: Environmental Health Authors: Magdal éna Rusková M ária Bučková Andrea Pu škárová Marianna C íchová Veronika Jansk á Adam Achs Zdeno Šubr Tom áš Kuchta Domenico Pangallo Source Type: research
Role of anti-polyethylene glycol (PEG) antibodies in the allergic reactions to PEG-containing Covid-19 vaccines: Evidence for immunogenicity of PEG
Conclusions: The small percentage of people who have extremelevels of anti-PEG Ab in their blood may be at increased risk for HSRs/anaphylaxis to PEGylated vaccines and other PEGylated injectables. This risk might be further increased by the anti-PEG immunogenicity of these vaccines. Screening for anti-PEG Ab "supercarriers" may help predicting reactors and thus preventing these adverse phenomena.PMID:37330369 | DOI:10.1016/j.vaccine.2023.06.009
Source: Vaccine - June 17, 2023 Category: Allergy & Immunology Authors: Gergely Tibor Kozma Tam ás Mészáros Petra Ber ényi R éka Facskó Zs ófia Patkó Csaba Zs Ol áh Adrienne Nagy Tam ás Gyula Fülöp Kathryn Anne Glatter Tam ás Radovits B éla Merkely J ános Szebeni Source Type: research
Characteristics of immune response profile in patients with immediate allergic and autoimmune urticarial reactions induced by SARS-CoV-2 vaccines
In conclusion, our results revealed that multiple vaccine components, inflammatory cytokines, and autoreactive IgG/IgE antibodies contribute to SARS-COV-2 vaccine-induced immediate allergic and autoimmune urticarial reactions.PMID:37245259 | PMC:PMC10183637 | DOI:10.1016/j.jaut.2023.103054
Source: Journal of Autoimmunity - May 28, 2023 Category: Allergy & Immunology Authors: Chuang-Wei Wang Chun-Bing Chen Chun-Wei Lu Wei-Ti Chen Rosaline Chung-Yee Hui Tsu-Man Chiu Min-Hui Chi Jing-Chi Lin Yu-Huei Huang Ya-Ching Chang Jennifer Wu Kuan-Yu Chen Yang Yu-Wei Lin Tzong-Yun Ger Jing Yi Lin Wan-Ting Tsai Yen-Ju Pan Wen-Hung Chung Source Type: research
A polyethylene glycol enhanced ligation-triggered self-priming isothermal amplification for the detection of SARS-CoV-2 D614G mutation
We presented a polyethylene glycol (PEG) enhanced ligation-triggered self-priming isothermal amplification (PEG-LSPA) for the detection D614G mutation in S-glycoprotein of SARS-CoV-2. PEG was employed to improve the ligation efficiency of this assay by constructing a molecular crowding environment. Two hairpin probes (H1 and H2) were designed to contain 18 nt and 20 nt target binding site at their 3' end and 5' end, respectively. In presence of target sequence, it complemented with H1 and H2 to trigger ligation by ligase under molecular crowding condition to form ligated H1-H2 duplex. Then 3' terminus of the H2 would be ex...
Source: Talanta - May 27, 2023 Category: Chemistry Authors: Luxin Yu Zibin Tang Yuanzhong Sun Hai Yi Yuebiao Tang Yangqing Zhong Dongchun Dian Yanguang Cong Houqi Wang Zhaoyang Xie Suhui He Zhangquan Chen Source Type: research
