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Morphological changes of macrophages and their potential contribution to tendon healing
Colloids Surf B Biointerfaces. 2021 Oct 1;209(Pt 1):112145. doi: 10.1016/j.colsurfb.2021.112145. Online ahead of print.ABSTRACTPoor healing ability and adhesion formation greatly hinder the recovery of injured tendon function. Previously, our local sustained gene delivery system by using cyclooxygenases (COX-1 and COX-2)-engineered miRNA plasmid/nanoparticles loaded hydrogel significantly inhibited adhesion formation and promoted tendon healing. The present study aims to study morphological changes of the macrophages in the healing tendons after above treatment with the hydrogel. Firstly, we assessed the therapeutic effect...
Source: Colloids and Surfaces - October 12, 2021 Category: Biotechnology Authors: Qian Qian Yang Luzhong Zhang You Lang Zhou Jin Bo Tang Source Type: research

Nano Carriers That Enable Co-Delivery of Chemotherapy and RNAi Agents for Treatment of Drug-Resistant Cancers.
Abstract Tumor cells exhibit drug resistant phenotypes that decrease the efficacy of chemotherapeutic treatments. The drug resistance has a genetic basis that is caused by an abnormal gene expression. There are several types of drug resistance: efflux pumps reducing the cellular concentration of the drug, alterations in membrane lipids that reduce cellular uptake, increased or altered drug targets, metabolic alteration of the drug, inhibition of apoptosis, repair of the damaged DNA, and alteration of the cell cycle checkpoints (Gottesman et al. , 2002, Holohan et al. , 2013). siRNA is used to silence the drug resi...
Source: Biotechnology Advances - June 9, 2014 Category: Biotechnology Authors: Tsouris V, Joo MK, Kim SH, Kwon IC, Won YY Tags: Biotechnol Adv Source Type: research

The application of the CRISPR-Cas9 genome editing machinery in food and agricultural science: Current status, future perspectives, and associated challenges.
Abstract The recent progress in genetic engineering has brought multiple benefits to the food and agricultural industry by enhancing essential characteristics of agronomic traits. Powerful tools in the field of genome editing, such as siRNA-mediated RNA interference for targeted suppression of gene expression and transcription activator-like effector nucleases (TALENs) and zinc-finger nucleases (ZFNs) for DNA repair have been widely used for commercial purposes. However, in the last few years, the discovery of the CRISPR-Cas9 system has revolutionized genome editing and has attracted attention as a powerful tool f...
Source: Biotechnology Advances - February 16, 2019 Category: Biotechnology Authors: Eş I, Gavahian M, Marti-Quijal FJ, Lorenzo JM, Khaneghah AM, Tsatsanis C, Kampranis SC, Barba FJ Tags: Biotechnol Adv Source Type: research

EPO could be regulated by HIF-1 and promote osteogenesis and accelerate bone repair.
This study was aimed to clarify the effect of erythropoietin on the repair of bone defect. We added the designated concentration of rhEPO to endothelial progenitor cells and marrow stromal cells, then detected its osteogenic and angiogenesis effects. The results showed that rhEPO promoted the proliferation of EPC and ST2 by promoting the mitosis without affecting cell apoptosis. The protein and mRNA levels of angiogenesis and osteogenic related factors exhibited higher expressions. Additionally, rhEPO encapsulated in PLGA scaffolds accelerated the new bone formation in rat calvaria bone defect model. Since the centre of bo...
Source: Artificial Cells, Nanomedicine and Biotechnology - December 19, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Inhibitory effects of msFGFR2c on the epithelial-to-mesenchymal transition of AE2 cells in pulmonary fibrosis.
Abstract In interstitial fibrosis, alveolar epithelial type II (AE2) cells fail to repair damaged epithelium. However, whether this dysfunction is related to fibroblast growth factor (FGF) signal pathway and how it affects the fibrotic process remains unclear. In our study, the medium of the human foetal lung fibroblast cell line MRC-5 (Med) can induce epithelial-to-mesenchymal transition (EMT) in AE2 cells, we also found that TGF-β in Med can induce FGF-2 and CTGF expression in AE2 cells. TGF-β or CTGF exposure trigger a FGFR2 subtype b to c transition which can be supressed by siRNA-CTGF. All together, since F...
Source: Biotechnology Letters - March 3, 2020 Category: Biotechnology Authors: Jieming G, Liu C, Yang Y, Mo S, Yang X, Wang J Tags: Biotechnol Lett Source Type: research

Chondroitin sulfate modified chitosan nanoparticles as an efficient and targeted gene delivery vehicle to chondrocytes
In conclusion, CH-CS nanoparticles can be considered as a candidate for gene therapy purposes in cartilage diseases.PMID:36049252 | DOI:10.1016/j.colsurfb.2022.112786
Source: Colloids and Surfaces - September 1, 2022 Category: Biotechnology Authors: Naghmeh Akbari Moghadam Fatemeh Bagheri Mohamadreza Baghaban Eslaminejad Source Type: research