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Total 1523 results found since Jan 2013.

Improving gene silencing of siRNAs via tricyclo-DNA modification.
Authors: Ittig D, Luisier S, Weiler J, Schümperli D, Leumann CJ Abstract Small interfering RNAs (siRNAs) can be exploited for the selective silencing of disease-related genes via the RNA interference (RNAi) machinery and therefore raise hope for future therapeutic applications. Especially chemically modified siRNAs are of interest as they are expected to convert lead siRNA sequences into effective drugs. To study the potential of tricyclo-DNA (tc-DNA) in this context we systematically incorporated tc-DNA units at various positions in a siRNA duplex targeted to the EGFP gene that was expressed in HeLa cells. Silenc...
Source: Artificial DNA: PNA and XNA - November 19, 2015 Category: Genetics & Stem Cells Tags: Artif DNA PNA XNA Source Type: research

Sequence-non-specific effects generated by various types of RNA interference triggers
Publication date: Available online 22 November 2015 Source:Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms Author(s): Marta Olejniczak, Martyna O. Urbanek, Edyta Jaworska, Lukasz Witucki, Michal W. Szczesniak, Izabela Makalowska, Wlodzimierz J. Krzyzosiak RNA interference triggers such as short interfering RNA (siRNA) or genetically encoded short hairpin RNA (shRNA) and artificial miRNA (sh-miR) are widely used to silence the expression of specific genes. In addition to silencing selected targets, RNAi reagents may induce various side effects, including immune responses. To determine the molecular...
Source: Biochimica et Biophysica Acta (BBA) Gene Regulatory Mechanisms - November 22, 2015 Category: Genetics & Stem Cells Source Type: research

A transgenerational role of the germline nuclear RNAi pathway in repressing heat stress-induced transcriptional activation in C. elegans
Conclusions: Our study demonstrated that a large number of the endogenous targets of the germline nuclear RNAi pathway in C. elegans are sensitive to heat-induced transcriptional activation. This effect at certain genomic loci including LTR retrotransposons is transgenerational. Germline nuclear RNAi antagonizes this temperature effect at the transcriptional level and therefore may play a key role in heat stress response in C. elegans.
Source: Epigenetics and Chromatin - January 15, 2016 Category: Genetics & Stem Cells Authors: Julie NiNatallia KalinavaEsteban ChenAlex HuangThi TrinhSam Gu Source Type: research

GSE73243 Expression data from U87 xenograft tissues
Contributor : Varda Shoshan-BarmatzSeries Type : Expression profiling by arrayOrganism : Homo sapiensU87 xenograft tumors treated with scrambled siRNA (Tas_73, Tas_78) or siRNA against VDAC1 (Tas_57, Tas_61)We used microarrays to detail the global effect of siRNA against VDAC1 on subcotenous xenograft U87 cells tumors
Source: GEO: Gene Expression Omnibus - February 1, 2016 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

Short interfering RNA induced generation and translation of stable 5’ mRNA cleavage intermediates
Publication date: Available online 16 June 2016 Source:Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms Author(s): Richa Singhania, Sandra Pavey, Elizabeth Payne, Wenyi Gu, Jennifer Clancy, Luqman Jubair, Thomas Preiss, Nicholas Saunders, Nigel A.J. McMillan Sequence-specific degradation of homologous mRNA is the main mechanism by which short-interfering RNAs (siRNAs) suppress gene expression. Generally, it is assumed that the mRNA fragments resulting from Ago-2 cleavage are rapidly degraded, thus making the transcript translation-incompetent. However, the molecular mechanisms involved in the pos...
Source: Biochimica et Biophysica Acta (BBA) Gene Regulatory Mechanisms - June 16, 2016 Category: Genetics & Stem Cells Source Type: research

Short interfering RNA induced generation and translation of stable 5 ’ mRNA cleavage intermediates
Publication date: Available online 16 June 2016 Source:Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms Author(s): Richa Singhania, Sandra Pavey, Elizabeth Payne, Wenyi Gu, Jennifer Clancy, Luqman Jubair, Thomas Preiss, Nicholas Saunders, Nigel A.J. McMillan Sequence-specific degradation of homologous mRNA is the main mechanism by which short-interfering RNAs (siRNAs) suppress gene expression. Generally, it is assumed that the mRNA fragments resulting from Ago-2 cleavage are rapidly degraded, thus making the transcript translation-incompetent. However, the molecular mechanisms involved in the pos...
Source: Biochimica et Biophysica Acta (BBA) Gene Regulatory Mechanisms - June 17, 2016 Category: Genetics & Stem Cells Source Type: research

Short interfering RNA induced generation and translation of stable 5 ′ mRNA cleavage intermediates
Publication date: August 2016 Source:Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, Volume 1859, Issue 8 Author(s): Richa Singhania, Sandra Pavey, Elizabeth Payne, Wenyi Gu, Jennifer Clancy, Luqman Jubair, Thomas Preiss, Nicholas Saunders, Nigel A.J. McMillan Sequence-specific degradation of homologous mRNA is the main mechanism by which short-interfering RNAs (siRNAs) suppress gene expression. Generally, it is assumed that the mRNA fragments resulting from Ago2 cleavage are rapidly degraded, thus making the transcript translation-incompetent. However, the molecular mechanisms involved in the post-cleav...
Source: Biochimica et Biophysica Acta (BBA) Gene Regulatory Mechanisms - August 6, 2016 Category: Genetics & Stem Cells Source Type: research

GSE74626 Differential gene expression in neuroblastoma cells after transfection with control siRNA, MYCN siRNA or TFAP4 siRNA.
Contributors : Chengyuan Xue ; Tao LiuSeries Type : Expression profiling by arrayOrganism : Homo sapiensWe analyed the gene expression profiles after knocking down MYCN or TFAP4. Results showed that transcription factor MYCN and TFAP4 commonly regulats a subset of genes that may contribute to neuroblastoma cells proliferation and migration.
Source: GEO: Gene Expression Omnibus - November 2, 2016 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

Multivalent methionine ‐functionalized biocompatible block copolymers for targeted small interfering RNA delivery and subsequent reversal effect on adriamycin resistance in human breast cancer cell line MCF‐7/ADR
ConclusionsThe synthesized polymer vector can deliver siRNA molecules into tumor cells and then reverse drug resistance by down regulation of P‐glycoprotein mRNA expression.
Source: The Journal of Gene Medicine - August 25, 2017 Category: Genetics & Stem Cells Authors: Yang Wu, Wei Zhang, Tinghan Li, Rong Ma, Dan Chen, Junying Zhang, Jianzhong Wu, Jinhai Tang Tags: RESEARCH ARTICLE Source Type: research

The role of long non-coding RNA PCA3 in epithelial ovarian carcinoma tumorigenesis and progression.
CONCLUSIONS: Research results show that lncRNA PCA3 may coordinate EOC tumorigenesis through disrupting miR-106b regulated gene expression. PCA3 may be a novel and important diagnostic biomarker and a valuable marker for prediction in the clinical care of epithelial ovarian carcinoma. PMID: 28864116 [PubMed - as supplied by publisher]
Source: Gene - August 29, 2017 Category: Genetics & Stem Cells Authors: Liu Y, Zong ZH, Guan X, Wang LL, Zhao Y Tags: Gene Source Type: research

GSE90582 Gene expression profiling of LRRC26-depleted HCC70 cells
Contributors : Yoshimasa Miyagawa ; Yosuke Matsushita ; Toyomasa KatagiriSeries Type : Expression profiling by arrayOrganism : Homo sapiensTo serach gene expression change under knockdown of LRRC26, Leucin Rich Repeat Containing 26 in human triple negative breast cancer cells HCC70, we performed genome-wide DNA microarray analysis and compared the expression levels of control-siRNA treated cells and LRRC26-siRNA treates cells at 48 to 72 h after siRNA transfection
Source: GEO: Gene Expression Omnibus - November 30, 2017 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

GSE90139 Differential gene expression analysis of MYCN-amplified neuroblastoma cells after transfection with Control siRNA, LYAR siRNA-6 or LYAR siRNA-10
Contributors : Tao Liu ; Bernard Atmadibrata ; Bing Lee ; Nicholas HoSeries Type : Expression profiling by arrayOrganism : Homo sapiensN-Myc oncoprotein induces neuroblastoma and exerts its biological effects through modulation of its target genes.We identified that MYCN modulates LYAR expression and we are interested to determine which signaling pathway LYAR regulates.
Source: GEO: Gene Expression Omnibus - December 31, 2017 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

GSE104015 Total and AGO1 immunoprecipitated small RNA from Arabidopsis thaliana wild-type and ago1-57 mutant
Contributors : Beno ît Derrien ; Marion Clavel ; Nicolas Baumberger ; Taichiro Iki ; Alexis Sarazin ; Thibaut Hacquard ; María R Ponce ; Olivier Voinnet ; Véronique Ziegler-Graff ; Hervé Vaucheret ; Olivier Voinnet ; José L Micol ; Pascal GenschikSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Arabidopsis thalianaIn Arabidopsis thaliana, ARGONAUTE1 (AGO1) plays a central role[AQ1] in microRNA (miRNA) and small interfering RNA (siRNA)- mediated silencing and is a key component in antiviral responses. The polerovirus F-box P0 protein triggers AGO1 degradation as a viral counterdefense. Her...
Source: GEO: Gene Expression Omnibus - June 22, 2018 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Arabidopsis thaliana Source Type: research

GSE102112 RNAseq analyze global transcriptome of changes between siIRF3 and siYAP in Gastric cancer cells
Contributors : Shi Jiao ; Jingmin GuanSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensPurpose: To investigate the signal crosstalk between IRF3 and YAP, RNA-sequencing (RNA-seq) was performed to analyze the genome-wide changes by the knockdown of IRF3 or YAP in HGC-27 cells.Methods: Total RNA was extracted from HGC-27 cell after transfection with negative control siRNA (n.c.), IRF3 siRNA (siIRF3) or YAP siRNA (siYAP). Then total RNA was quality controlled and quantified using an Agilent 2100 Bioanalyzer. After converting to cDNA and building library, high-throughput sequencing was per...
Source: GEO: Gene Expression Omnibus - August 1, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Dual-layer transposon repression in heads of Drosophila melanogaster.
Abstract piRNA-mediated repression of transposable elements (TE) in the germline limits the accumulation of heritable mutations caused by their transposition in the genome. It is not clear whether the piRNA pathway plays a functional role in adult, non-gonadal tissues in Drosophila melanogaster. To address this question, we first analyzed the small RNA content of adult Drosophila melanogaster heads. We found that varying amount of piRNA-sized, ping-pong positive molecules in heads correlates with contamination by gonadal tissue during RNA extraction, suggesting that most of piRNAs detected in head sequencing libra...
Source: RNA - September 14, 2018 Category: Genetics & Stem Cells Authors: van den Beek M, da Silva B, Pouch J, Ali Chaouche MEA, Carré C, Antoniewski C Tags: RNA Source Type: research