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Hepatitis B virus X protein boosts hepatocellular carcinoma progression by downregulating microRNA-137.
CONCLUSION: Overexpression of miR-137 blocks HCC cell proliferation in HBx-siRNA-treated MHCC97H cells by targeting Notch1. This study may offer novel target for HCC treatment. PMID: 32527447 [PubMed - in process]
Source: Pathology, Research and Practice - May 31, 2020 Category: Pathology Authors: Gao Y, Gu J, Wang Y, Fu D, Zhang W, Zheng G, Wang X Tags: Pathol Res Pract Source Type: research

Novel Robust in Vitro Hepatitis B Virus Infection Model Using Fresh Human Hepatocytes Isolated from Humanized Mice.
Abstract The molecular mechanisms underlying the hepatitis B virus (HBV) life cycle are poorly understood because of the lack of appropriate in vitro infection models. Herein, we report a highly effective in vitro HBV infection system using fresh human hepatocytes (HHs) isolated from chimeric mice with humanized livers. After the inoculation of sera collected from HBV-infected chimeric mice or patients to HHs, we measured levels of HBV DNA, mRNA, covalently closed circular DNA, and viral protein expression in HHs. We investigated the neutralization activity of hepatitis B immune globulin and the effects of siRNA...
Source: The American Journal of Pathology - March 17, 2015 Category: Pathology Authors: Ishida Y, Yamasaki C, Yanagi A, Yoshizane Y, Fujikawa K, Watashi K, Abe H, Wakita T, Hayes CN, Chayama K, Tateno C Tags: Am J Pathol Source Type: research

A rational study for identification of highly effective siRNAs against hepatitis B virus.
In this study, we demonstrate that an assembly of results generated from different siRNA designing programs could provide clusters of predicting sites that aided selection of potent siRNAs. Based on the clusters, three siRNA target sites were selected on a conserved RNA region of hepatitis B virus (HBV), known as HBV post-transcriptional regulatory element (HBV PRE) at nucleotide positions 1317-1337, 1357-1377 and 1644-1664. All three chosen siRNAs driven by H1 promoter were highly effective and could drastically decrease expression of HBV transcripts (core, surface and X) and surface protein without induction of interfero...
Source: Experimental and Molecular Pathology - June 19, 2014 Category: Pathology Authors: Thongthae N, Payungporn S, Poovorawan Y, T-Thienprasert NP Tags: Exp Mol Pathol Source Type: research