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Total 442 results found since Jan 2013.
Inhibition of RELM-β prevents hypoxia-induced overproliferation of human pulmonary artery smooth muscle cells by reversing PLC-mediated KCNK3 decline
Publication date: Available online 8 February 2020Source: Life SciencesAuthor(s): Linlin Han, Nannan Song, Xiaomin Hu, Afang Zhu, Xin Wei, Jinmin Liu, Shiying Yuan, Weike Mao, Xiangdong ChenAbstractAimsAlthough resistin-like molecule β (RELM-β) is involved in the pathological processes of various lung diseases, such as pulmonary inflammation, asthma and fibrosis, its potential roles in hypoxic pulmonary arterial hypertension (PAH) remain largely unknown. The study aims to investigate whether RELM-β contributes to hypoxia-induced excessive proliferation of human pulmonary artery smooth muscle cells (PASMCs) and to explor...
Source: Life Sciences - February 9, 2020 Category: Biology Source Type: research
Host factor prioritization for pan-viral genetic perturbation screens using random intercept models and network propagation
by Simon Dirmeier, Christopher D ächert, Martijn van Hemert, Ali Tas, Natacha S. Ogando, Frank van Kuppeveld, Ralf Bartenschlager, Lars Kaderali, Marco Binder, Niko Beerenwinkel
Genetic perturbation screens using RNA interference (RNAi) have been conducted successfully to identify host factors that are essential for the life cycle of bacteria or viruses. So far, most published studies identified host factors primarily for single pathogens. Furthermore, often only a small subset of genes, e.g., genes encoding kinases, have been targeted. Identification of host factors on a pan-pathogen level, i.e., genes that are crucial ...
Source: PLoS Computational Biology - February 9, 2020 Category: Biology Authors: Simon Dirmeier Source Type: research
A stress response p38 MAP kinase inhibitor SB202190 promoted TFEB/TFE3-dependent autophagy and lysosomal biogenesis independent of p38
Publication date: Available online 28 January 2020Source: Redox BiologyAuthor(s): Chuanbin Yang, Zhou Zhu, Benjamin Chun-Kit Tong, Ashok Iyaswamy, Wen-Jian Xie, Yu Zhu, Sravan Gopalkrishnashetty Sreenivasmurthy, Krishnamoorthi Senthilkumar, King-Ho Cheung, Ju-Xian Song, Hong-Jie Zhang, Min LiAbstractTFEB (transcription factor EB) and TFE3 (transcription factor E3) are “master regulators” of autophagy and lysosomal biogenesis. The stress response p38 mitogen-activated protein (MAP) kinases affects multiple intracellular responses including inflammation, cell growth, differentiation, cell death, senescence, tumorigenesis...
Source: Redox Biology - January 29, 2020 Category: Biology Source Type: research
Hydroxychloroquine suppresses lung tumorigenesis via inducing FoxO3a nuclear translocation through STAT3 inactivation
ConclusionsTaken together, this study suggests that STAT3/FoxO3a/p27Kip1 signaling pathway is involved in the anticancer effects of HCQ, and provides preliminary evidence for therapeutic prospects of HCQ alone in LUAD.Graphical abstract
Source: Life Sciences - January 29, 2020 Category: Biology Source Type: research
Syndecan-1 regulates extracellular matrix expression in keloid fibroblasts via TGF-β1/Smad and MAPK signaling pathways
Publication date: Available online 15 January 2020Source: Life SciencesAuthor(s): Jing Cui, Shan Jin, Chenglong Jin, Zhehu JinAbstractAimsThe present study aimed to explore the effect of syndecan-1 on keloid fibroblasts.Main methodsImmunohistochemistry and Western blot were employed to assess the expression of syndecan-1. Primary cultured keloid fibroblasts were transfected with syndecan-1 siRNA. The function of syndecan-1 on the proliferation of keloid fibroblasts was investigated through Cell Counting Kit-8 (CCK-8) and flow cytometry. Extracellular matrix, TGF-β1/Smad, and MAPK related proteins were evaluated by Western...
Source: Life Sciences - January 17, 2020 Category: Biology Source Type: research
Berberine mitigates high glucose-induced podocyte apoptosis by modulating autophagy via the mTOR/P70S6K/4EBP1 pathway
This study aimed to investigate the characteristics and mechanism of autophagy on podocyte apoptosis under high glucose (HG) conditions and further explore the effect of berberine on podocyte autophagy, apoptosis and the potential mechanism.Materials and methodsThe levels of LC3II/I in podocytes stimulated with HG were detected at 0, 2, 4, 8, 12, 24, 36 and 48 h by western blotting. CCK-8 was used to detect the viability of podocytes. The level of autophagy was detected by western blotting, transmission electron microscopy and immunofluorescence. Podocyte apoptosis was analysed by using Hoechst staining, western blotting...
Source: Life Sciences - January 9, 2020 Category: Biology Source Type: research
Dihydroartemisinin inhibits endothelial cell tube formation by suppression of the STAT3 signaling pathway
Publication date: Available online 24 December 2019Source: Life SciencesAuthor(s): Peng Gao, Li-li Wang, Jing Liu, Fengyun Dong, Wei Song, Lin Liao, Bei Wang, Wenqian Zhang, Xia Zhou, Qi Xie, Rong Sun, Ju LiuAbstractAimsEndothelial cell (EC) tube formation is crucial for tumor angiogenesis, which becomes a target for chemotherapy. The anti-malaria agent dihydroartemisinin (DHA) inhibited tumor growth and angiogenesis. The aim of this study was to investigate the effects of DHA on EC tube formation and the underlying mechanisms.Materials and methodsHuman umbilical vein endothelial cells (HUVECs) were cultured with different...
Source: Life Sciences - December 26, 2019 Category: Biology Source Type: research
BCLAF1 promotes cell proliferation, invasion and drug-resistance though targeting lncRNA NEAT1 in hepatocellular carcinoma
Publication date: Available online 20 December 2019Source: Life SciencesAuthor(s): Shao-Jiao Mou, Peng-Fei Yang, Yi-Pin Liu, Ning Xu, Wei-Wei Jiang, Wen-Jing YueAbstractAimsIn the present research, we aimed to investigate the effect of Bcl-2-associated transcription factor 1 (BCLAF1) on hepatocellular carcinoma and further explore the special molecular mechanism.Main methodsThe expression of BCLAF1 was analyzed in tumor tissues and different hepatocellular cancer cell lines by real-time RT-PCR and Western blot. Cell proliferation and invasion was explored using MTT and Transwell assay respectively. In addition, luciferase ...
Source: Life Sciences - December 20, 2019 Category: Biology Source Type: research
TEX19 promotes ovarian carcinoma progression and is a potential target for epitope vaccine immunotherapy
This study aimed to reveal the role of TEX19 in ovarian cancer (OC) and searched for potential candidate epitope peptides of TEX19 to facilitate clinical application.Main methodsTEX19 levels were evaluated by immunohistochemistry (IHC) in 98 human ovarian tissue samples. The correlation of TEX19 levels with patients' clinicopathological features was assessed. Quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting analysis were utilized to detect TEX19 levels in ovarian cell lines and TEX19-deficient cells. The level of TEX19 in OVCAR-3 and A2780 was knocked down by small interfering RNA (siRNA), an...
Source: Life Sciences - December 14, 2019 Category: Biology Source Type: research
NALP3 orchestrates cellular bioenergetics to facilitate non-small cell lung cancer cell growth
This study was to unravel the mechanism of NALP3 on modulating NSCLC cancer cell growth.MethodsIHC and immuno-blot were performed to analyze expression of NALP3 and indicated molecules. CCK-8 and xenograft nude mice assay were used to evaluate cell growth in vitro and in vivo. Bioenergetics assay was performed to measure OXPHOS and aerobic glycolysis. siRNA and shRNA were constructed to knockdown endogenous NALP3 and DNMT1. Co-immunoprecipitation was applied to confirm the interaction between NALP3 and DMAP1. BioProfile FLEX analyzer and Lactate Reagent Kit were used to measure relative level glucose uptake and lactate pro...
Source: Life Sciences - December 14, 2019 Category: Biology Source Type: research
Molecular characterization of a RNA polymerase (RNAP) II (DNA directed) polypeptide H (POLR2H) in Pacific white shrimp (Litopenaeus vannamei) and its role in response to high-pH stress
Publication date: Available online 9 December 2019Source: Fish & Shellfish ImmunologyAuthor(s): Zhi-Zhan Luo, Hui-Ming Sun, Jing-Wen Guo, Peng Luo, Chao-Qun Hu, Wen Huang, Hu ShuAbstractRNA polymerase (RNAP) II (DNA-directed) (POLR2) genes are essential for cell viability under environmental stress and for the transfer of biological information from DNA to RNA. However, the function and characteristics of POLR2 genes in crustaceans are still unknown. In the present study, a POLR2H cDNA was isolated from Pacific white shrimp (Litopenaeus vannamei) and designated as Lv-POLR2H. The full-length Lv-POLR2H cDNA is 772 bp in leng...
Source: Fish and Shellfish Immunology - December 11, 2019 Category: Biology Source Type: research
HMGB1 upregulates NF-kB by inhibiting IKB-α and associates with diabetic retinopathy
This study aimed to investigate the effect of high-mobility group box 1 (HMGB1) protein and its mechanism in diabetic retinopathy (DR) were investigated.Main methodsHuman retinal endothelial cells (HREC) were uesd for chip-seq. Sprague Dawley (SD) rats were randomly divided into control group, HMGB1 group, diabetes mellitus (DM) combined with HMGB1 siRNA group, and DM group. Next, eyeballs were removed and retinas were detached for western blot. The DM model of cell was built by increasing the glucose concentration in cell culture medium. The regulation of HMGB1 was achieved by short hairpin (sh)-HMGB1 transfection, then, ...
Source: Life Sciences - December 7, 2019 Category: Biology Source Type: research
Amentoflavone suppresses amyloid β1–42 neurotoxicity in Alzheimer's disease through the inhibition of pyroptosis
ConclusionAF inhibits Aβ1-42-induced neurotoxicity in animal and cellular models through AMPK/GSK3β-mediated pyroptosis suppression. Our results highlight AF as a clinical compound for the prevention and treatment of AD.
Source: Life Sciences - November 16, 2019 Category: Biology Source Type: research
Amentoflavone suppresses Amyloid β1–42 neurotoxicity in Alzheimer’s disease through the inhibition of pyroptosis
ConclusionAF inhibits Aβ1-42-induced neurotoxicity in animal and cellular models through AMPK/GSK3β-mediated pyroptosis suppression. Our results highlight AF as a clinical compound for the prevention and treatment of AD.
Source: Life Sciences - November 10, 2019 Category: Biology Source Type: research
NLRP3 inflammasome mediate palmitate-induced endothelial dysfunction
This study aimed to investigate the role of NLRP3 (NOD-like receptor pyrin domain containing-3) inflammasome in FFA induced endothelial dysfunction.Main methodsHUVECs were transfected with NLRP3 siRNA and then stimulated with LPS and palmitate. C57 BL/6J mice transfected with NLRP3 Lenti-Virus were fed with a high-fat diet (HFD). The levels of NLRP3 inflammasome, AMPKα (AMP-activated protein kinase), endothelial nitric oxide synthase (eNOS) and the activity of the insulin signal pathway, in endothelial cells were determined via Western blotting. Endothelial function was determined by measuring the level of endothelium-dep...
Source: Life Sciences - November 7, 2019 Category: Biology Source Type: research
