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Polydopamine-assisted chlorhexidine immobilization on medical grade stainless steel 316L: Apatite formation and in vitro osteoblastic evaluation.
This study aimed to investigate the ability of CHX coating to promote apatite layer, osteoblast cells viability, adhesion, osteogenic differentiation and mineralization. Stainless steel 316L disks were pre-treated, grafted with a polydopamine film and immobilized with different concentrations of CHX (10-30mM). The apatite layer formation was determined through an in vitro simulated body fluid (SBF) test by ATR-FTIR and SEM-EDX analyses. The osteoblastic evaluations including cells viability, cells adhesion, osteogenic differentiation and mineralization were assessed with human fetal osteoblast cells through MTT assay, morp...
Source: Annals of Anatomy - July 23, 2018 Category: Anatomy Authors: Daud NM, Al-Ashwal RH, Kadir MRA, Saidin S Tags: Ann Anat Source Type: research

In vitro Antimicrobial Activity of Robenidine, Ethylenediaminetetraacetic Acid and Polymyxin B Nonapeptide Against Important Human and Veterinary Pathogens
Conclusion The results of our study demonstrate that robenidine is not suitable as a sole antimicrobial agent for the treatment of Gram-negative pathogen infections due to the lack of activity against the majority of Gram-negative isolates except for A. baumannii and A. calcoaceticus. However, we demonstrated in vitro efficacy against all selected Gram-negative organisms when robenidine was tested in combination with EDTA or PMBN, including against multidrug-resistant strains. Therefore, robenidine may be an appropriate candidate as a component of a combination preparation for the treatment of otitis externa in dogs. This...
Source: Frontiers in Microbiology - April 24, 2019 Category: Microbiology Source Type: research

Antimicrobial Efficacy of Silver Diamine Fluoride against < em > Enterococcus faecalis < /em > : A Systematic Review of < em > In Vitro < /em > Studies
Conclusions. Within the limitations of the systematic review and the studies reviewed, SDF may be effective against E. faecalis and therefore could be used as an intracanal medicament and/or irrigant to prevent reinfections of the root canals and improve the outcomes of endodontic treatment. However, animal and clinical studies should be carried out to determine the efficacy of SDF in endodontics. Trial Registration. The protocol for this review was registered on PROSPERO. Registration number: CRD42021224741.PMID:36567909 | PMC:PMC9788898 | DOI:10.1155/2022/6544292
Source: Biomed Res - December 26, 2022 Category: Research Authors: Juzer Shabbir Zohaib Khurshid Muhammad Sohail Zafar Waqas Ahmed Farooqui Eisha Imran Shariq Najeeb Sompop Bencharit Source Type: research

Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production
Braz Dent J. 2022 Nov-Dec;33(6):36-43. doi: 10.1590/0103-6440202205195.ABSTRACTThe aim of this study was to evaluate in vitro the effect of calcium hydroxide [Ca(OH)2], 2% chlorhexidine gel (CHX) on macrophages (RAW 264.7) to produce pro-inflammatory cytokines and nitric oxide after pretreatment with lipoteichoic acid (LTA) of Enterococcus faecalis. Forty-eight human single-rooted teeth were instrumented with R25.08 (RECIPROC) and sterilized by gamma irradiation. LTA was inoculated in the root canal of each specimen for 96 hours. Specimens were instrumented with 40.06 and 50.05 (RECIPROC) and medicated with: I) Pyrogen-fre...
Source: Braz Dent J - December 8, 2022 Category: Dentistry Authors: Luciane Dias de Oliveira Felipe Eduardo de Oliveira B árbara Araujo Hatje Marcia Carneiro Valera Cl áudio Antonio Talge Carvalho Amjad Abu Hasna Source Type: research

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