In vitro Antimicrobial Activity of Robenidine, Ethylenediaminetetraacetic Acid and Polymyxin B Nonapeptide Against Important Human and Veterinary Pathogens

Conclusion The results of our study demonstrate that robenidine is not suitable as a sole antimicrobial agent for the treatment of Gram-negative pathogen infections due to the lack of activity against the majority of Gram-negative isolates except for A. baumannii and A. calcoaceticus. However, we demonstrated in vitro efficacy against all selected Gram-negative organisms when robenidine was tested in combination with EDTA or PMBN, including against multidrug-resistant strains. Therefore, robenidine may be an appropriate candidate as a component of a combination preparation for the treatment of otitis externa in dogs. This study provides proof of concept of drug repurposing in the field of veterinary otology and would represent a good example of antimicrobial stewardship when the compound is ultimately developed and used clinically in dogs. Finally, the additive and synergistic effects of robenidine in combination with EDTA or PMBN provide an important and novel development pathways for treatment of additional antimicrobial-resistant Gram-negative pathogens in animals and humans. Author Contributions MK contributed to the study design, MIC and combination testing, kill time, biofilm assay, analyzed results, and wrote the preliminary manuscript. HP contributed towards kill time assay, MIC testing, biofilm assay, and data analysis. WC performed testing on the robenidine and EDTA combination, biofilm assay, and data analysis. JS participated in cell cytotoxicity assays. AO con...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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Microbial Drug Resistance, Ahead of Print.
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