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Specialty: Drugs & Pharmacology
Cancer: Leukemia
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Total 66 results found since Jan 2013.
Suppression of protein tyrosine phosphatase PTPN22 gene induces apoptosis in T-cell leukemia cell line (Jurkat) through the AKT and ERK pathways
In this study, Jurkat cells were transfected with specific PTPN22 siRNA. Relative PTPN22 mRNA expression was measured by Quantitative Real-time PCR. Western blotting was performed to determine the protein levels of PTPN22, AKT, P-AKT, ERK, and P-ERK. The cytotoxic effects of PTPN22 siRNA were determined using the MTT assay. Apoptosis was quantified using TUNEL assay and flow cytometry. Results showed that in Jurkat cells after transfection with PTPN22 siRNA, the expression of PTPN22 in both mRNA and protein levels was effectively reduced. Moreover, siRNA transfection induced apoptosis on the viability of T-cell acute leuke...
Source: Biomedicine and Pharmacotherapy - December 6, 2016 Category: Drugs & Pharmacology Source Type: research
Suppression of protein tyrosine phosphatase PTPN22 gene induces apoptosis in T-cell leukemia cell line (Jurkat) through the AKT and ERK pathways.
In this study, Jurkat cells were transfected with specific PTPN22 siRNA. Relative PTPN22 mRNA expression was measured by Quantitative Real-time PCR. Western blotting was performed to determine the protein levels of PTPN22, AKT, P-AKT, ERK, and P-ERK. The cytotoxic effects of PTPN22 siRNA were determined using the MTT assay. Apoptosis was quantified using TUNEL assay and flow cytometry. Results showed that in Jurkat cells after transfection with PTPN22 siRNA, the expression of PTPN22 in both mRNA and protein levels was effectively reduced. Moreover, siRNA transfection induced apoptosis on the viability of T-cell acute leuke...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - December 5, 2016 Category: Drugs & Pharmacology Authors: Baghbani E, Baradaran B, Pak F, Mohammadnejad L, Shanehbandi D, Mansoori B, Khaze V, Montazami N, Mohammadi A, Kokhaei P Tags: Biomed Pharmacother Source Type: research
The influence of HOXA5-specific siRNA on the expression of Livin and Smac proteins.
CONCLUSIONS: HOXA5-specific siRNA effectively silenced the HOXA5 gene expression and down-regulation of HOXA5 induced the down-regulation of Livin protein expression and up-regulation of Smac protein. We suggest the HOXA5 gene to be considered as the new target for acute leukemia gene therapy.
PMID: 27460741 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - July 29, 2016 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
MEK inhibitor CI-1040 induces apoptosis in acute myeloid leukemia cells in vitro.
CONCLUSIONS: These results demonstrate that CI-1040 induce apoptosis of U-937 cells and might be a new therapeutic option for the treatment of AML.
PMID: 27249593 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - June 3, 2016 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
Current attempts to implement siRNA-based RNAi in leukemia models.
Abstract
Leukemias arise from genetic alterations in normal hematopoietic stem or progenitor cells, leading to abnormal blood population with transformed cells. With the advent of RNAi and its pharmacological mediator siRNA, it has become possible to downregulate specific drivers causing leukemias. In this review, we present unique aspects of RNAi-mediated therapy and delivery technologies. Recent updates on molecular targets and delivery systems are discussed emanating from in vitro cell models and preclinical animal models. We conclude with a view on the future of RNAi in leukemia therapy, emphasizing possible m...
Source: Drug Discovery Today - April 24, 2016 Category: Drugs & Pharmacology Authors: Uludağ H, Landry B, Valencia-Serna J, Remant-Bahadur KC, Meneksedağ-Erol D Tags: Drug Discov Today Source Type: research
PRAME promotes in vitro leukemia cells death by regulating S100A4/p53 signaling.
CONCLUSIONS: Our results suggest that the leukemias expressing high levels of PRAME has a favorable prognosis. PRAME promotes in vitro leukemia cells death by regulating S100A4/p53 signaling.
PMID: 27049257 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - April 8, 2016 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
Ginkgolide B protects human umbilical vein endothelial cells against xenobiotic injuries via PXR activation.
CONCLUSION: Ginkgolide B exerts anti-apoptotic and anti-inflammatory effects on endothelial cells via PXR activation, suggesting that a PXR-mediated endothelial detoxification program may be important for protecting endothelial cells from xeno- and endobiotic-induced injuries.
PMID: 26775663 [PubMed - as supplied by publisher]
Source: Acta Pharmacologica Sinica - January 18, 2016 Category: Drugs & Pharmacology Authors: Zhou T, You WT, Ma ZC, Liang QD, Tan HL, Xiao CR, Tang XL, Zhang BL, Wang YG, Gao Y Tags: Acta Pharmacol Sin Source Type: research
Knockdown of PRAME enhances adriamycin-induced apoptosis in chronic myeloid leukemia cells.
CONCLUSIONS: PRAME is responsible for the inherent low levels of spontaneous apoptosis in K562 cells. The combination of PRAME siRNA with ADR induced more intense apoptosis compared with each single treatment. PRAME siRNA in combination with ADR is well tolerated and shows greater efficacy than either agent alone in mouse xenograft models.
PMID: 26744874 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - January 15, 2016 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
In vitro application of RNA interference to silence livin gene expression to induce apoptosis in leukemia cells.
CONCLUSIONS: Caspase-3 activity in cells transfected with siRNA was significantly elevated compared to the cells in the non-transfected groups (p < 0.05).
PMID: 26636528 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - December 6, 2015 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
Baicalin inhibiting cerebral ischemia /hypoxia-induced neuronal apoptosis via MRTF-A-mediated transactivity.
Abstract
Baicalin has been shown to provide the neuroprotective effect by alleviating cerebral ischemia injury. However, little's known about the underlying mechanism. Here, a cerebral artery occlusion (MACO)/reperfusion rat model and rat primary cortical neuron culture exposed to hydrogen peroxide (H2O2) were established to evaluate the effect of baicalin on ischemia-induced neuronal apoptosis. We found baicalin can significantly less neurological deficit and reduced infarct volume in vivo. And it efficiently inhibited neuronal apoptosis in vivo and vitro, which was especially characterized by the enhancing of tr...
Source: European Journal of Pharmacology - October 17, 2015 Category: Drugs & Pharmacology Authors: Zheng WX, Cao XL, Wang F, Wu FJ, Wang J, Ying TZ, Xiao W, Zhang Y, Xing H, Dong W, Xu SQ, Min ZL, Hu XM Tags: Eur J Pharmacol Source Type: research
Reversal of chemoresistance with small interference RNA (siRNA) in etoposide resistant acute myeloid leukemia cells (HL-60).
CONCLUSIONS: Our results indicate that product of the ABCB1 gene have effective role in resistance to etoposide in acute myeloid leukemia cells.
PMID: 26463638 [PubMed - in process]
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - October 1, 2015 Category: Drugs & Pharmacology Authors: Kachalaki S, Baradaran B, Majidi J, Yousefi M, Shanehbandi D, Mohammadinejad S, Mansoori B Tags: Biomed Pharmacother Source Type: research
Reversal of chemoresistance with small interference RNA (siRNA) in etoposide resistant acute myeloid leukemia cells (HL-60)
Conclusions Our results indicate that product of the ABCB1 gene have effective role in resistance to etoposide in acute myeloid leukemia cells.
Source: Biomedicine and Pharmacotherapy - September 26, 2015 Category: Drugs & Pharmacology Source Type: research
Induction of cytosine arabinoside-resistant human myeloid leukemia cell death through autophagy regulation by hydroxychloroquine.
Abstract
We investigated the effects of the autophagy inhibitor hydroxychloroquine (HCQ) on cell death of cytosine arabinoside (Ara-C)-resistant human acute myeloid leukemia (AML) cells. Ara-C-sensitive (U937, AML-2) and Ara-C-resistant (U937/AR, AML-2/AR) human AML cell lines were used to evaluate HCQ-regulated cytotoxicity, autophagy, and apoptosis as well as effects on cell death-related signaling pathways. We found that HCQ-induced dose- and time-dependent cell death in Ara-C-resistant cells compared to Ara-C-sensitive cell lines. The extent of cell death and features of HCQ-induced autophagic markers includin...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - July 1, 2015 Category: Drugs & Pharmacology Authors: Kim Y, Eom JI, Jeung HK, Jang JE, Kim JS, Cheong JW, Kim YS, Min YH Tags: Biomed Pharmacother Source Type: research
Induction of cytosine arabinoside-resistant human myeloid leukemia cell death through autophagy regulation by hydroxychloroquine
Publication date: Available online 30 May 2015 Source:Biomedicine & Pharmacotherapy Author(s): Yundeok Kim , Ju-In Eom , Hoi-Kyung Jeung , Ji Eun Jang , Jin Seok Kim , June-Won Cheong , Young Sam Kim , Yoo Hong Min We investigated the effects of the autophagy inhibitor hydroxychloroquine (HCQ) on cell death of cytosine arabinoside (Ara-C)-resistant human acute myeloid leukemia (AML) cells. Ara-C (cytosine arabinoside)-sensitive (U937, AML-2) and Ara-C-resistant (U937/AR, AML-2/AR) human myeloid leukemia cell lines were used to evaluate HCQ-regulated cytotoxicity, autophagy, and apoptosis, as well as effects on ce...
Source: Biomedicine and Pharmacotherapy - June 20, 2015 Category: Drugs & Pharmacology Source Type: research
Resveratrol induces apoptosis of human chronic myelogenous leukemia cells in vitro through p38 and JNK-regulated H2AX phosphorylation
amp; Cheng-rong Lu
Source: Acta Pharmacologica Sinica - March 3, 2015 Category: Drugs & Pharmacology Authors: Xin-pin WuMin XiongCheng-shan XuLian-ning DuanYa-qiong DongYuan LuoTian-hui NiuCheng-rong Lu Tags: resveratrol chronic myelogenous leukemia K562 cell H2AX apoptosis p38 JNK siRNA SB202190 SP600125 Source Type: research
