The grapevine miR827a regulates the synthesis of stilbenes by targeting VqMYB14 and gives rise to susceptibility in plant immunity
In this study, a microRNA (miRNA; miR827a), which negatively regulates its target gene VqMYB14, a key regulatory role in the synthesis of stilbenes, was identified in Vitis quinquangularis (V. quinquangularis) using transcriptome sequencing. Using overexpression and silencing approaches, we found that miR827a regulates the synthesis of stilbenes by targeting VqMYB14. We used flagellin N-terminal 22-amino-acid peptide (flg22), the representative elicitor in plant basal immunity, as the elicitor to verify whether miR827a is involved in the basal immunity of V. quinquangularis. Furthermore, the promoter activity of miR827a wa...
Source: TAG. Theoretical and Applied Genetics - April 6, 2024 Category: Genetics & Stem Cells Authors: Yangyang Luo Linxia Wang Jie Zhu Jingwen Tian Lin You Qin Luo Jia Li Qian Yao Dong Duan Source Type: research

Identification of a novel marker and its associated laccase gene for regulating ear length in tropical and subtropical maize lines
This study revealed the identification of a novel gene, Zm00001d042906, that regulates maize ear length by modulating lignin synthesis and reported a molecular marker for selecting maize lines with elongated ears. Maize ear length has garnered considerable attention due to its high correlation with yield. In this study, six maize inbred lines of significant importance in maize breeding were used as parents. The temperate maize inbred line Ye107, characterized by a short ear, was crossed with five tropical or subtropical inbred lines featuring longer ears, creating a multi-parent population displaying significant variations...
Source: TAG. Theoretical and Applied Genetics - April 5, 2024 Category: Genetics & Stem Cells Authors: Yaqi Bi Fuyan Jiang Yudong Zhang Ziwei Li Tianhui Kuang Ranjan K Shaw Muhammad Adnan Kunzhi Li Xingming Fan Source Type: research

Identification of a novel marker and its associated laccase gene for regulating ear length in tropical and subtropical maize lines
This study revealed the identification of a novel gene, Zm00001d042906, that regulates maize ear length by modulating lignin synthesis and reported a molecular marker for selecting maize lines with elongated ears. Maize ear length has garnered considerable attention due to its high correlation with yield. In this study, six maize inbred lines of significant importance in maize breeding were used as parents. The temperate maize inbred line Ye107, characterized by a short ear, was crossed with five tropical or subtropical inbred lines featuring longer ears, creating a multi-parent population displaying significant variations...
Source: TAG. Theoretical and Applied Genetics - April 5, 2024 Category: Genetics & Stem Cells Authors: Yaqi Bi Fuyan Jiang Yudong Zhang Ziwei Li Tianhui Kuang Ranjan K Shaw Muhammad Adnan Kunzhi Li Xingming Fan Source Type: research

Identification and functional analysis of a chromosome 2D fragment harboring TaFPF1 gene with the potential for yield improvement using a late heading wheat mutant
Theor Appl Genet. 2024 Apr 3;137(4):92. doi: 10.1007/s00122-024-04593-1.ABSTRACTA chromosome fragment influencing wheat heading and grain size was identified using mapping of m406 mutant. The study of TaFPF1 in this fragment provides more insights into wheat yield improvement. In recent years, wheat production has faced formidable challenges driven by rapid population growth and climate change, emphasizing the importance of improving specific agronomic traits such as heading date, spike length, and grain size. To identify potential genes for improving these traits, we screened a wheat EMS mutant library and identified a mu...
Source: TAG. Theoretical and Applied Genetics - April 3, 2024 Category: Genetics & Stem Cells Authors: Lifen Wu Guangrong Li Danping Li Chunhao Dong Xueying Zhang Lichao Zhang Zujun Yang Xiuying Kong Chuan Xia Jingtang Chen Xu Liu Source Type: research

Identification of superior and rare haplotypes to optimize branch number in soybean
In this study, an integrated approach with a genome-wide association study (GWAS) and haplotype and candidate gene analyses was used to determine the detailed genetic basis of branch number across a diverse set of soybean accessions. The GWAS revealed a total of eleven SNPs significantly associated with branch number across three environments using the five GWAS models. Based on the consistency of the SNP detection in multiple GWAS models and environments, seven genomic regions within the physical distance of ± 202.4 kb were delineated as stable QTLs. Of these QTLs, six QTLs were novel, viz., qBN7, qBN13, qBN16, qBN18, qB...
Source: TAG. Theoretical and Applied Genetics - April 3, 2024 Category: Genetics & Stem Cells Authors: Hui Yu Javaid Akhter Bhat Candong Li Beifang Zhao Moran Bu Zhirui Zhang Tai Guo Xianzhong Feng Source Type: research

Identification and functional analysis of a chromosome 2D fragment harboring TaFPF1 gene with the potential for yield improvement using a late heading wheat mutant
Theor Appl Genet. 2024 Apr 3;137(4):92. doi: 10.1007/s00122-024-04593-1.ABSTRACTA chromosome fragment influencing wheat heading and grain size was identified using mapping of m406 mutant. The study of TaFPF1 in this fragment provides more insights into wheat yield improvement. In recent years, wheat production has faced formidable challenges driven by rapid population growth and climate change, emphasizing the importance of improving specific agronomic traits such as heading date, spike length, and grain size. To identify potential genes for improving these traits, we screened a wheat EMS mutant library and identified a mu...
Source: TAG. Theoretical and Applied Genetics - April 3, 2024 Category: Genetics & Stem Cells Authors: Lifen Wu Guangrong Li Danping Li Chunhao Dong Xueying Zhang Lichao Zhang Zujun Yang Xiuying Kong Chuan Xia Jingtang Chen Xu Liu Source Type: research

Identification of superior and rare haplotypes to optimize branch number in soybean
In this study, an integrated approach with a genome-wide association study (GWAS) and haplotype and candidate gene analyses was used to determine the detailed genetic basis of branch number across a diverse set of soybean accessions. The GWAS revealed a total of eleven SNPs significantly associated with branch number across three environments using the five GWAS models. Based on the consistency of the SNP detection in multiple GWAS models and environments, seven genomic regions within the physical distance of ± 202.4 kb were delineated as stable QTLs. Of these QTLs, six QTLs were novel, viz., qBN7, qBN13, qBN16, qBN18, qB...
Source: TAG. Theoretical and Applied Genetics - April 3, 2024 Category: Genetics & Stem Cells Authors: Hui Yu Javaid Akhter Bhat Candong Li Beifang Zhao Moran Bu Zhirui Zhang Tai Guo Xianzhong Feng Source Type: research

Mapping and validation of Fusarium wilt race 2 resistance QTL from Citrullus amarus line USVL246-FR2
Theor Appl Genet. 2024 Mar 31;137(4):91. doi: 10.1007/s00122-024-04595-z.ABSTRACTFon race 2 resistant QTLs were identified on chromosomes 8 and 9. Families homozygous for resistance alleles at a haplotype of three KASP markers had 42% lower disease severity than those with susceptible alleles in an independent, interspecific validation population confirming their utility for introgression of Fusarium wilt resistance. Fusarium oxysporum f. sp. niveum (Fon) race 2 causes Fusarium wilt in watermelon and threatens watermelon production worldwide. Chemical management options are not effective, and no resistant edible watermelon...
Source: TAG. Theoretical and Applied Genetics - March 31, 2024 Category: Genetics & Stem Cells Authors: Venkata Rao Ganaparthi Patrick Wechter Amnon Levi Sandra E Branham Source Type: research

Mapping and validation of Fusarium wilt race 2 resistance QTL from Citrullus amarus line USVL246-FR2
Theor Appl Genet. 2024 Mar 31;137(4):91. doi: 10.1007/s00122-024-04595-z.ABSTRACTFon race 2 resistant QTLs were identified on chromosomes 8 and 9. Families homozygous for resistance alleles at a haplotype of three KASP markers had 42% lower disease severity than those with susceptible alleles in an independent, interspecific validation population confirming their utility for introgression of Fusarium wilt resistance. Fusarium oxysporum f. sp. niveum (Fon) race 2 causes Fusarium wilt in watermelon and threatens watermelon production worldwide. Chemical management options are not effective, and no resistant edible watermelon...
Source: TAG. Theoretical and Applied Genetics - March 31, 2024 Category: Genetics & Stem Cells Authors: Venkata Rao Ganaparthi Patrick Wechter Amnon Levi Sandra E Branham Source Type: research

Mapping and validation of Fusarium wilt race 2 resistance QTL from Citrullus amarus line USVL246-FR2
Theor Appl Genet. 2024 Mar 31;137(4):91. doi: 10.1007/s00122-024-04595-z.ABSTRACTFon race 2 resistant QTLs were identified on chromosomes 8 and 9. Families homozygous for resistance alleles at a haplotype of three KASP markers had 42% lower disease severity than those with susceptible alleles in an independent, interspecific validation population confirming their utility for introgression of Fusarium wilt resistance. Fusarium oxysporum f. sp. niveum (Fon) race 2 causes Fusarium wilt in watermelon and threatens watermelon production worldwide. Chemical management options are not effective, and no resistant edible watermelon...
Source: TAG. Theoretical and Applied Genetics - March 31, 2024 Category: Genetics & Stem Cells Authors: Venkata Rao Ganaparthi Patrick Wechter Amnon Levi Sandra E Branham Source Type: research

Fine mapping and candidate gene analysis of qSRC3 controlling the silk color in maize (Zea mays L.)
In this study, a F2 population arising from the hybridization of the single-segment substitution line qSRC3MT1 with red silk, carrying an introgressed allele from teosinte (Zea mays ssp. mexicana), and the recurrent maize inbred line Mo17, characterized by light green silk, was utilized for fine mapping. We found that the red silk trait is controlled by a semi-dominant genetic locus known as qSRC3, and its expression is susceptible to light-mediated inhibition. Moreover, qSRC3 explained 68.78% of the phenotypic variance and was delimited to a 133.2 kb region, which includes three genes. Subsequent expression analyses revea...
Source: TAG. Theoretical and Applied Genetics - March 30, 2024 Category: Genetics & Stem Cells Authors: Xueying Wang Yang Zhou Chong You Jinchang Yang Zhengjie Chen Dengguo Tang Jixing Ni Peng Li Le Wang Kaili Zhu Wujiao Deng Haimei Wu Ruifan Bao Zhiqin Liu Pengxu Meng Sijia Yang Tingzhao Rong Jian Liu Source Type: research

Fine mapping and candidate gene analysis of qSRC3 controlling the silk color in maize (Zea mays L.)
In this study, a F2 population arising from the hybridization of the single-segment substitution line qSRC3MT1 with red silk, carrying an introgressed allele from teosinte (Zea mays ssp. mexicana), and the recurrent maize inbred line Mo17, characterized by light green silk, was utilized for fine mapping. We found that the red silk trait is controlled by a semi-dominant genetic locus known as qSRC3, and its expression is susceptible to light-mediated inhibition. Moreover, qSRC3 explained 68.78% of the phenotypic variance and was delimited to a 133.2 kb region, which includes three genes. Subsequent expression analyses revea...
Source: TAG. Theoretical and Applied Genetics - March 30, 2024 Category: Genetics & Stem Cells Authors: Xueying Wang Yang Zhou Chong You Jinchang Yang Zhengjie Chen Dengguo Tang Jixing Ni Peng Li Le Wang Kaili Zhu Wujiao Deng Haimei Wu Ruifan Bao Zhiqin Liu Pengxu Meng Sijia Yang Tingzhao Rong Jian Liu Source Type: research

A diverse panel of 755 bread wheat accessions harbors untapped genetic diversity in landraces and reveals novel genetic regions conferring powdery mildew resistance
Theor Appl Genet. 2024 Mar 27;137(4):88. doi: 10.1007/s00122-024-04582-4.ABSTRACTA bread wheat panel reveals rich genetic diversity in Turkish, Pakistani and Iranian landraces and novel resistance loci to diverse powdery mildew isolates via subsetting approaches in association studies. Wheat breeding for disease resistance relies on the availability and use of diverse genetic resources. More than 800,000 wheat accessions are globally conserved in gene banks, but they are mostly uncharacterized for the presence of resistance genes and their potential for agriculture. Based on the selective reduction of previously assembled ...
Source: TAG. Theoretical and Applied Genetics - March 27, 2024 Category: Genetics & Stem Cells Authors: Rebecca Leber Matthias Heuberger Victoria Widrig Esther Jung Etienne Paux Beat Keller Javier S ánchez-Martín Source Type: research

Identification of QTLs for symbiotic nitrogen fixation and related traits in a soybean recombinant inbred line population
Theor Appl Genet. 2024 Mar 27;137(4):89. doi: 10.1007/s00122-024-04591-3.ABSTRACTThe genetic architecture of symbiotic N fixation and related traits was investigated in the field. QTLs were identified for percent N derived from the atmosphere, shoot [N] and C to N ratio. Soybean [Glycine max (L.) Merr.] is cultivated worldwide and is the most abundant source of plant-based protein. Symbiotic N2 fixation (SNF) in legumes such as soybean is of great importance; however, yields may still be limited by N in both high yielding and stressful environments. To better understand the genetic architecture of SNF and facilitate the de...
Source: TAG. Theoretical and Applied Genetics - March 27, 2024 Category: Genetics & Stem Cells Authors: C Bennet Krueger Jeffery D Ray James R Smith Arun Prabhu Dhanapal Muhammad Arifuzzaman Fei Gao Felix B Fritschi Source Type: research

A diverse panel of 755 bread wheat accessions harbors untapped genetic diversity in landraces and reveals novel genetic regions conferring powdery mildew resistance
Theor Appl Genet. 2024 Mar 27;137(4):88. doi: 10.1007/s00122-024-04582-4.ABSTRACTA bread wheat panel reveals rich genetic diversity in Turkish, Pakistani and Iranian landraces and novel resistance loci to diverse powdery mildew isolates via subsetting approaches in association studies. Wheat breeding for disease resistance relies on the availability and use of diverse genetic resources. More than 800,000 wheat accessions are globally conserved in gene banks, but they are mostly uncharacterized for the presence of resistance genes and their potential for agriculture. Based on the selective reduction of previously assembled ...
Source: TAG. Theoretical and Applied Genetics - March 27, 2024 Category: Genetics & Stem Cells Authors: Rebecca Leber Matthias Heuberger Victoria Widrig Esther Jung Etienne Paux Beat Keller Javier S ánchez-Martín Source Type: research