TAG. Theoretical and Applied Genetics This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

A novel QTL conferring Fusarium crown rot resistance on chromosome 2A in a wheat EMS mutant
In this study, genetic analysis was conducted for a wheat EMS mutant C549 which showed stable resistance to FCR at seedling stage. A total of 10 QTL were detected on chromosomes 1A, 2A, 3B, 4A, 6B, and 7B using a population of 138 F7 recombinant inbred lines (RILs) derived from a cross between C549 and a Chinese germplasm 3642. A novel locus Qfcr.cau-2A, which accounted for up to 24.42% of the phenotypic variation with a LOD value of 12.78, was consistently detected across all six trials conducted. Furthermore, possible effects of heading date (HD) and plant height on FCR severity were also investigated in the mapping popu...
Source: TAG. Theoretical and Applied Genetics - February 13, 2024 Category: Genetics & Stem Cells Authors: Xiangru Xu Yuqing Su Jiatian Yang Jinlong Li Yutian Gao Cong Li Xingyi Wang Lulu Gou Zhi Zheng Chaojie Xie Jian Ma Jun Ma Source Type: research

A conditional mutation in a wheat (Triticum aestivum L.) gene regulating root morphology
Theor Appl Genet. 2024 Feb 12;137(2):48. doi: 10.1007/s00122-024-04555-7.ABSTRACTCharacterisation and genetic mapping of a key gene defining root morphology in bread wheat. Root morphology is central to plants for the efficient uptake up of soil water and mineral nutrients. Here we describe a conditional mutant of hexaploid wheat (Triticum aestivum L.) that when grown in soil with high Ca2+ develops a larger rhizosheath accompanied with shorter roots than the wild type. In wheat, rhizosheath size is a reliable surrogate for root hair length and this was verified in the mutant which possessed longer root hairs than the wild...
Source: TAG. Theoretical and Applied Genetics - February 12, 2024 Category: Genetics & Stem Cells Authors: Deying Zeng Brett Ford Jaroslav Dole žel Miroslava Karafi átová Mathew J Hayden Tina M Rathjen Timothy S George Lawrie K Brown Peter R Ryan Filomena A Pettolino Ulrike Mathesius Emmanuel Delhaize Source Type: research

Different wheat loci are associated to heritable free asparagine content in grain grown under different water and nitrogen availability
Theor Appl Genet. 2024 Feb 9;137(2):46. doi: 10.1007/s00122-024-04551-x.ABSTRACTKEY MESSAGE: Different wheat QTLs were associated to the free asparagine content of grain grown in four different conditions. Environmental effects are a key factor when selecting for low acrylamide-forming potential. The amount of free asparagine in grain of a wheat genotype determines its potential to form harmful acrylamide in derivative food products. Here, we explored the variation in the free asparagine, aspartate, glutamine and glutamate contents of 485 accessions reflecting wheat worldwide diversity to define the genetic architecture go...
Source: TAG. Theoretical and Applied Genetics - February 9, 2024 Category: Genetics & Stem Cells Authors: M élanie Lavoignat C édric Cassan Pierre P étriacq Yves Gibon Emmanuel Heumez C éline Duque Philippe Momont Renaud Rincent Justin Blancon Catherine Ravel Jacques Le Gouis Source Type: research

Fine mapping of Ae-Ps4.5, a major locus for resistance to pathotype III of Aphanomyces euteiches in pea
This study aimed to confirm the major effect of Ae-Ps4.5 on A. euteiches pathotype III, refine its interval, and identify candidate genes underlying the QTL. QTL mapping on an updated genetic map from the Puget × 90-2079 pea recombinant inbred line population identified Ae-Ps4.5 in a 0.8-cM confidence interval with a high effect (R2 = 89%) for resistance to the Ae109 reference strain of A. euteiches (pathotype III) under controlled conditions. However, the QTL mapping did not detect Ae-Ps4.5 for resistance to the RB84 reference strain of A. euteiches (pathotype I). Screening 224-pea BC5F2 plant progeny derived from three ...
Source: TAG. Theoretical and Applied Genetics - February 9, 2024 Category: Genetics & Stem Cells Authors: Cl ément Lavaud Ang élique Lesné Th éo Leprévost Marie-Laure Pilet-Nayel Source Type: research

Breeding progress of nitrogen use efficiency of cereal crops, winter oilseed rape and peas in long-term variety trials
Theor Appl Genet. 2024 Feb 8;137(2):45. doi: 10.1007/s00122-023-04521-9.ABSTRACTGrain yield and NUE increased over time while nitrogen yield did not drop significantly despite reduced nitrogen input. Selection for grain and nitrogen yield is equivalent to selection for NUE. Breeding and registration of improved varieties with high yield, processing quality, disease resistance and nitrogen use efficiency (NUE) are of utmost importance for sustainable crop production to minimize adverse environmental impact and contribute to food security. Based on long-term variety trials of cereals, winter oilseed rape and grain peas teste...
Source: TAG. Theoretical and Applied Genetics - February 8, 2024 Category: Genetics & Stem Cells Authors: F Laidig T Feike C Lichthardt A Schierholt H P Piepho Source Type: research

Mutation in BrFLS encoding flavonol synthase induced anthocyanin accumulation in Chinese cabbage
Theor Appl Genet. 2024 Feb 7;137(2):44. doi: 10.1007/s00122-024-04552-w.ABSTRACTBrFLS mutation promoted anthocyanin accumulation in Chinese cabbage, which was verified in four allelic mutants. Chinese cabbage is a major vegetable crop in Eastern Asia. Anthocyanin-rich vibrantly colored varieties are increasingly favored by consumers for their higher nutritional and aesthetic value compared to the typical green varieties of Chinese cabbage. Herein, we identified an anthocyanin accumulation mutant aam1 from a mutant library of EMS-mutagenized Chinese cabbage DH line 'FT', which appeared partial purple on leaves, bolting stem...
Source: TAG. Theoretical and Applied Genetics - February 7, 2024 Category: Genetics & Stem Cells Authors: Jiaqi Zou Shengnan Huang Yue Gao Wei Fu Zhiyong Liu Hui Feng Meidi Zhang Source Type: research

Dissecting the genetic basis of Fusarium crown rot resistance in wheat by genome wide association study
In this study, we attempted to detect loci conferring FCR resistance through a new seedling inoculation assay. A total of 223 wheat accessions from different geography origins were used to assemble an association panel for GWAS analysis. Four genotypes including Heng 4332, Luwanmai, Pingan 998 and Yannong 24 showed stable resistance to FCR. A total of 54 SNPs associated with FCR resistance were identified. Among the 10 putative QTLs represented by these SNPs, seven QTLs on chromosome 2B, 3A, 3D, 4A, 7A and 7B were novel and were consistently detected in at least two of the three trials conducted. Qfcr.cau.3D-3, which was t...
Source: TAG. Theoretical and Applied Genetics - February 6, 2024 Category: Genetics & Stem Cells Authors: Jinlong Li Shanshan Zhai Xiangru Xu Yuqing Su Jiazheng Yu Yutian Gao Jiatian Yang Zhi Zheng Baoyun Li Qixin Sun Chaojie Xie Jun Ma Source Type: research

Precise colocalization of sorghum's major chilling tolerance locus with Tannin1 due to tight linkage drag rather than antagonistic pleiotropy
Theor Appl Genet. 2024 Feb 3;137(2):42. doi: 10.1007/s00122-023-04534-4.ABSTRACTChilling tolerance in crops can increase resilience through longer growing seasons, drought escape, and nitrogen use efficiency. In sorghum (Sorghum bicolor [L.] Moench), breeding for chilling tolerance has been stymied by coinheritance of the largest-effect chilling tolerance locus, qSbCT04.62, with the major gene underlying undesirable grain proanthocyanidins, WD40 transcriptional regulator Tannin1. To test if this coinheritance is due to antagonistic pleiotropy of Tannin1, we developed and studied near-isogenic lines (NILs) carrying chilling...
Source: TAG. Theoretical and Applied Genetics - February 3, 2024 Category: Genetics & Stem Cells Authors: Anthony Schuh Terry J Felderhoff Sandeep Marla Geoffrey P Morris Source Type: research

Map-based cloning and functional analysis of a major quantitative trait locus, BolC.Pb9.1, controlling clubroot resistance in a wild Brassica relative (Brassica macrocarpa)
In this study, we constructed populations using B2013 and broccoli line 90196. CR in B2013 is quantitatively inherited, and a major QTL, BolC.Pb9.1, was identified on C09 using QTL-seq and linkage analysis. The BolC.Pb9.1 was finely mapped to a 56 kb genomic region using F2:3 populations. From the target region, the candidate BoUGT76C2 showed nucleotide variations between the parents, and was inducible in response to P. brassicae infection. We generated BoUGT76C2 overexpression lines in the 90196 background, which showed significantly enhanced resistance to P. brassicae compared to the WT line, suggesting that BoUGT76C2 co...
Source: TAG. Theoretical and Applied Genetics - February 2, 2024 Category: Genetics & Stem Cells Authors: Xiaoli Zhang Fengqing Han Zhansheng Li Zhenghua Wen Wenjuan Cheng Xiaozheng Shan Deling Sun Yumei Liu Source Type: research

Comparative transcriptome analysis and genetic dissection of vegetative branching traits in foxtail millet (Setaria italica)
Theor Appl Genet. 2024 Jan 31;137(2):39. doi: 10.1007/s00122-023-04524-6.ABSTRACTTwo major genetic loci, qTN5.1 and qAB9.1, were identified and finely mapped to the 255 Kb region with one potential candidate gene for tiller number and the 521 Kb region with eight candidate genes for axillary branch number, respectively. Vegetative branching including tillering and axillary branching are vital traits affecting both the plant architecture and the biomass in cereal crops. However, the mechanism underlying the formation of vegetative branching in foxtail millet is largely unknown. Here, a foxtail millet cultivar and its bushy ...
Source: TAG. Theoretical and Applied Genetics - January 31, 2024 Category: Genetics & Stem Cells Authors: Tianpeng Liu Xueying Liu Jihong He Kongjun Dong Lei Zhang Yawei Li Ruiyu Ren Tianyu Yang Source Type: research

An integrated QTL mapping and transcriptome sequencing provides further molecular insights and candidate genes for stem strength in rapeseed (Brassica napus L.)
Theor Appl Genet. 2024 Jan 31;137(2):38. doi: 10.1007/s00122-023-04535-3.ABSTRACTWe detected the major QTL- qSR.A07, which regulated stem strength and was fine-mapped to 490 kb. BnaA07G0302800ZS and BnaA07G0305700ZS as the candidate functional genes were identified at qSR.A07 locus. The stem's mechanical properties reflect its ability to resist lodging. In rapeseed (Brassica napus L.), although stem lodging negatively affects yield and generates harvesting difficulties, the molecular regulation of stem strength remains elusive. Hence, this study aimed to unravel the main loci and molecular mechanisms governing rapeseed ste...
Source: TAG. Theoretical and Applied Genetics - January 31, 2024 Category: Genetics & Stem Cells Authors: Zhengshu Tian Xinfa Wang Xiaoling Dun Kaiqin Zhao Hanzhong Wang Lijun Ren Source Type: research

Genetic gains in IRRI's rice salinity breeding and elite panel development as a future breeding resource
Theor Appl Genet. 2024 Jan 31;137(2):37. doi: 10.1007/s00122-024-04545-9.ABSTRACTEstimating genetic gains and formulating a future salinity elite breeding panel for rice pave the way for developing better high-yielding salinity tolerant lines with enhanced genetic gains. Genetic gain is a crucial parameter to check the breeding program's success and help optimize future breeding strategies for enhanced genetic gains. To estimate the genetic gains in IRRI's salinity breeding program and identify the best genotypes based on high breeding values for grain yield (kg/ha), we analyzed the historical data from the trials conducte...
Source: TAG. Theoretical and Applied Genetics - January 31, 2024 Category: Genetics & Stem Cells Authors: Apurva Khanna Mahender Anumalla Joie Ramos Ma Teresa Sta Cruz Margaret Catolos Andres Godwin Sajise Glenn Gregorio Shalabh Dixit Jauhar Ali Md Rafiqul Islam Vikas Kumar Singh Md Akhlasur Rahman Hasina Khatun Daniel Joseph Pisano Sankalp Bhosale Waseem Hus Source Type: research

An analysis of lncRNAs related to fiber quality and the discovery of their target genes in a Gossypium hirsutum line with Gossypium mustelinum introgression
Theor Appl Genet. 2024 Jan 31;137(2):40. doi: 10.1007/s00122-024-04541-z.ABSTRACTAnalysis of fiber quality lncRNAs and their target genes from a pair of Gossypium mustelinum near-isogenic lines provide new prospects for improving the fiber quality of Upland cotton. Long noncoding RNAs (lncRNAs) are an important part of genome transcription and play roles in a wide range of biological processes in plants. In this research, a pair of near-isogenic cotton lines, namely, a Gossypium mustelinum introgression line (IL9) with outstanding fiber quality and its recurrent Upland cotton parent (PD94042), were used as the experimental...
Source: TAG. Theoretical and Applied Genetics - January 31, 2024 Category: Genetics & Stem Cells Authors: Baohua Wang Meijun Ji Hui Fang Haijing Gu Teame Gereziher Mehari Jinlei Han Wenxiang Feng Xuehan Huo Jingxia Zhang Yu Chen Jun Zhang Allah Ditta Muhammad K R Khan Andrew H Paterson Peng W Chee Kai Wang Source Type: research

GenoBaits ®WheatplusEE: a targeted capture sequencing panel for quick and accurate identification of wheat-Thinopyrum derivatives
This study aimed to develop a high-resolution wheat-Thinopyrum elongatum array, named GenoBaits®WheatplusEE, to trace alien genetic information by genotyping using a target sequencing system. This GenoBaits®WheatplusEE array included 90,000 capture probes derived from two species and integrated into one chip, with 10,000 and 80,000 originating from wheat and Th. elongatum, respectively. The capture probes were strategically positioned in genes and evenly distributed across the genome, facilitating the development of a roadmap for identifying each alien gene. The array was applied to the high-throughput identification of ...
Source: TAG. Theoretical and Applied Genetics - January 30, 2024 Category: Genetics & Stem Cells Authors: Pingchuan Deng Xin Du Yanzhen Wang Xiaoying Yang Xiaofang Cheng Chenxi Huang Tingting Li Tingdong Li Chunhuan Chen Jixin Zhao Changyou Wang Xinlun Liu Zengrong Tian Wanquan Ji Source Type: research