Rht12b, a widely used ancient allele of TaGA2oxA13, reduces plant height and enhances yield potential in wheat
Theor Appl Genet. 2023 Nov 21;136(12):253. doi: 10.1007/s00122-023-04502-y.ABSTRACTWe identified a new wheat dwarfing allele Rht12b conferring reduced height and higher grain yield, pinpointed its causal variations, developed a breeding-applicable marker, and traced its origin and worldwide distribution. Plant height control is essential to optimize lodging resistance and yield gain in crops. RHT12 is a reduced height (Rht) locus that is identified in a mutationally induced dwarfing mutant and encodes a gibberellin 2-oxidase TaGA2oxA13. However, the artificial dwarfing allele is not used in wheat breeding due to excessive ...
Source: TAG. Theoretical and Applied Genetics - November 22, 2023 Category: Genetics & Stem Cells Authors: Yingjie Bian Lingli Li Xiuling Tian Dengan Xu Mengjing Sun Faji Li Lina Xie Siyang Liu Bingyan Liu Xianchun Xia Zhonghu He Shuanghe Cao Source Type: research

Rht12b, a widely used ancient allele of TaGA2oxA13, reduces plant height and enhances yield potential in wheat
Theor Appl Genet. 2023 Nov 21;136(12):253. doi: 10.1007/s00122-023-04502-y.ABSTRACTWe identified a new wheat dwarfing allele Rht12b conferring reduced height and higher grain yield, pinpointed its causal variations, developed a breeding-applicable marker, and traced its origin and worldwide distribution. Plant height control is essential to optimize lodging resistance and yield gain in crops. RHT12 is a reduced height (Rht) locus that is identified in a mutationally induced dwarfing mutant and encodes a gibberellin 2-oxidase TaGA2oxA13. However, the artificial dwarfing allele is not used in wheat breeding due to excessive ...
Source: TAG. Theoretical and Applied Genetics - November 22, 2023 Category: Genetics & Stem Cells Authors: Yingjie Bian Lingli Li Xiuling Tian Dengan Xu Mengjing Sun Faji Li Lina Xie Siyang Liu Bingyan Liu Xianchun Xia Zhonghu He Shuanghe Cao Source Type: research

Rht12b, a widely used ancient allele of TaGA2oxA13, reduces plant height and enhances yield potential in wheat
Theor Appl Genet. 2023 Nov 21;136(12):253. doi: 10.1007/s00122-023-04502-y.ABSTRACTWe identified a new wheat dwarfing allele Rht12b conferring reduced height and higher grain yield, pinpointed its causal variations, developed a breeding-applicable marker, and traced its origin and worldwide distribution. Plant height control is essential to optimize lodging resistance and yield gain in crops. RHT12 is a reduced height (Rht) locus that is identified in a mutationally induced dwarfing mutant and encodes a gibberellin 2-oxidase TaGA2oxA13. However, the artificial dwarfing allele is not used in wheat breeding due to excessive ...
Source: TAG. Theoretical and Applied Genetics - November 22, 2023 Category: Genetics & Stem Cells Authors: Yingjie Bian Lingli Li Xiuling Tian Dengan Xu Mengjing Sun Faji Li Lina Xie Siyang Liu Bingyan Liu Xianchun Xia Zhonghu He Shuanghe Cao Source Type: research

Models to estimate genetic gain of soybean seed yield from annual multi-environment field trials
Theor Appl Genet. 2023 Nov 21;136(12):252. doi: 10.1007/s00122-023-04470-3.ABSTRACTSimulations demonstrated that estimates of realized genetic gain from linear mixed models using regional trials are biased to some degree. Thus, we recommend multiple selected models to obtain a range of reasonable estimates. Genetic improvements of discrete characteristics are obvious and easy to demonstrate, while quantitative traits require reliable and accurate methods to disentangle the confounding genetic and non-genetic components. Stochastic simulations of soybean [Glycine max (L.) Merr.] breeding programs were performed to evaluate ...
Source: TAG. Theoretical and Applied Genetics - November 21, 2023 Category: Genetics & Stem Cells Authors: Matheus D Krause Hans-Peter Piepho Kaio O G Dias Asheesh K Singh William D Beavis Source Type: research

Combined linkage analysis and association mapping identifies genomic regions associated with yield-related and drought-tolerance traits in wheat (Triticum aestivum L.)
In this study, two water treatments (well-watered, WW, and rain-fed water stress, WS) were applied, and five yield-related agronomic traits (plant height, PH; spike length, SL; spikelet number per spike, SNPS; kernel number per spike, KNPS; thousand kernel weight, TKW) and drought response values (DRVs) were used to characterize the drought sensitivity of each accession. Association mapping was performed on an association panel of 304 accessions, and linkage analysis was applied to a doubled haploid (DH) population of 152 lines. Eleven co-localized genomic regions associated with yield traits and DRV were identified in bot...
Source: TAG. Theoretical and Applied Genetics - November 20, 2023 Category: Genetics & Stem Cells Authors: Jie Guo Jiahui Guo Long Li Xionghui Bai Xiaoyu Huo Weiping Shi Lifeng Gao Keli Dai Ruilian Jing Chenyang Hao Source Type: research

Identification of QTLs for resistance to 10 pathotypes of Plasmodiophora brassicae in Brassica oleracea cultivar ECD11 through genotyping-by-sequencing
Theor Appl Genet. 2023 Nov 20;136(12):249. doi: 10.1007/s00122-023-04483-y.ABSTRACTTwo major quantitative trait loci (QTLs) and five minor QTLs for 10 pathotypes were identified on chromosomes C01, C03, C04 and C08 through genotyping-by-sequencing from Brassica oleracea. Clubroot caused by Plasmodiophora brassicae is an important disease in brassica crops. Managing clubroot disease of canola on the Canadian prairie is challenging due to the continuous emergence of new pathotypes. Brassica oleracea is considered a major source of quantitative resistance to clubroot. Genotyping-by-sequencing (GBS) was performed in the parent...
Source: TAG. Theoretical and Applied Genetics - November 20, 2023 Category: Genetics & Stem Cells Authors: Md Masud Karim Fengqun Yu Source Type: research

Genome-wide association mapping combined with gene-based haplotype analysis identify a novel gene for shoot length in rice (Oryza sativa L.)
In this study, a genome-wide association study of SL was performed in a diverse rice collection consisting of 391 accessions in two years, using phenotypes generated by different cultivation methods according to the production practice, and a total of twenty-four QTLs for SL were identified. Among them, the novel QTL qSL-1f on chromosome 1 could be stably detected across all three cultivation methods in the whole population and indica subpopulation. Through gene-based haplotype analysis of the annotated genes within the putative region of qSL-1f, and validated by gene expression and knockout transgenic experiments, LOC_Os0...
Source: TAG. Theoretical and Applied Genetics - November 20, 2023 Category: Genetics & Stem Cells Authors: Tifeng Yang Jingfang Dong Junliang Zhao Longting Zhang Lian Zhou Wu Yang Yamei Ma Jian Wang Hua Fu Jiansong Chen Wenhui Li Haifei Hu Xianya Jiang Ziqiang Liu Bin Liu Shaohong Zhang Source Type: research

Combined linkage analysis and association mapping identifies genomic regions associated with yield-related and drought-tolerance traits in wheat (Triticum aestivum L.)
In this study, two water treatments (well-watered, WW, and rain-fed water stress, WS) were applied, and five yield-related agronomic traits (plant height, PH; spike length, SL; spikelet number per spike, SNPS; kernel number per spike, KNPS; thousand kernel weight, TKW) and drought response values (DRVs) were used to characterize the drought sensitivity of each accession. Association mapping was performed on an association panel of 304 accessions, and linkage analysis was applied to a doubled haploid (DH) population of 152 lines. Eleven co-localized genomic regions associated with yield traits and DRV were identified in bot...
Source: TAG. Theoretical and Applied Genetics - November 20, 2023 Category: Genetics & Stem Cells Authors: Jie Guo Jiahui Guo Long Li Xionghui Bai Xiaoyu Huo Weiping Shi Lifeng Gao Keli Dai Ruilian Jing Chenyang Hao Source Type: research

Identification of QTLs for resistance to 10 pathotypes of Plasmodiophora brassicae in Brassica oleracea cultivar ECD11 through genotyping-by-sequencing
Theor Appl Genet. 2023 Nov 20;136(12):249. doi: 10.1007/s00122-023-04483-y.ABSTRACTTwo major quantitative trait loci (QTLs) and five minor QTLs for 10 pathotypes were identified on chromosomes C01, C03, C04 and C08 through genotyping-by-sequencing from Brassica oleracea. Clubroot caused by Plasmodiophora brassicae is an important disease in brassica crops. Managing clubroot disease of canola on the Canadian prairie is challenging due to the continuous emergence of new pathotypes. Brassica oleracea is considered a major source of quantitative resistance to clubroot. Genotyping-by-sequencing (GBS) was performed in the parent...
Source: TAG. Theoretical and Applied Genetics - November 20, 2023 Category: Genetics & Stem Cells Authors: Md Masud Karim Fengqun Yu Source Type: research

Mapping of Rf20(t), a minor fertility restorer gene for rice wild abortive cytoplasmic male sterility in the maintainer line 'Zhenshan97B'
This study observed fertile plants in WA-TianfengA/Zhenshan97B//TianfengB population, indicating that the maintainer line 'Zhenshan97B' should carry Rfs for WA-type CMS. Several advanced backcross populations were generated with the genetic background of the 'WA-TianfengA,' and the pollen fertility levels of the backcrossed individuals in BC3F1, BC4F1 and BC4F2 populations are governed by a new gene, Rf20(t), from 'Zhenshan97B.' Employing bulk segregant analysis of fertile and sterile pools from the BC4F1 population, Rf20(t) was genetically mapped to a candidate region on chromosome 10. Subsequently, Rf20(t) was located be...
Source: TAG. Theoretical and Applied Genetics - November 18, 2023 Category: Genetics & Stem Cells Authors: Yang Liu Zuopeng Xu Yuanyue Du Benxi Huang Meng Li Lijia Zhang Xiangqiang Zhao Qiaoquan Liu Shuzhu Tang Honggen Zhang Source Type: research

Mapping of Rf20(t), a minor fertility restorer gene for rice wild abortive cytoplasmic male sterility in the maintainer line 'Zhenshan97B'
This study observed fertile plants in WA-TianfengA/Zhenshan97B//TianfengB population, indicating that the maintainer line 'Zhenshan97B' should carry Rfs for WA-type CMS. Several advanced backcross populations were generated with the genetic background of the 'WA-TianfengA,' and the pollen fertility levels of the backcrossed individuals in BC3F1, BC4F1 and BC4F2 populations are governed by a new gene, Rf20(t), from 'Zhenshan97B.' Employing bulk segregant analysis of fertile and sterile pools from the BC4F1 population, Rf20(t) was genetically mapped to a candidate region on chromosome 10. Subsequently, Rf20(t) was located be...
Source: TAG. Theoretical and Applied Genetics - November 18, 2023 Category: Genetics & Stem Cells Authors: Yang Liu Zuopeng Xu Yuanyue Du Benxi Huang Meng Li Lijia Zhang Xiangqiang Zhao Qiaoquan Liu Shuzhu Tang Honggen Zhang Source Type: research

Meta-QTL analysis in wheat: progress, challenges and opportunities
Theor Appl Genet. 2023 Nov 17;136(12):247. doi: 10.1007/s00122-023-04490-z.ABSTRACTWheat, an important cereal crop globally, faces major challenges due to increasing global population and changing climates. The production and productivity are challenged by several biotic and abiotic stresses. There is also a pressing demand to enhance grain yield and quality/nutrition to ensure global food and nutritional security. To address these multifaceted concerns, researchers have conducted numerous meta-QTL (MQTL) studies in wheat, resulting in the identification of candidate genes that govern these complex quantitative traits. MQT...
Source: TAG. Theoretical and Applied Genetics - November 17, 2023 Category: Genetics & Stem Cells Authors: Divya Sharma Anita Kumari Priya Sharma Anupma Singh Anshu Sharma Zahoor Ahmad Mir Uttam Kumar Sofora Jan M Parthiban Reyazul Rouf Mir Pradeep Bhati Anjan Kumar Pradhan Aakash Yadav Dwijesh Chandra Mishra Neeraj Budhlakoti Mahesh C Yadav Kiran B Gaikwad Am Source Type: research

Meta-QTL analysis in wheat: progress, challenges and opportunities
Theor Appl Genet. 2023 Nov 17;136(12):247. doi: 10.1007/s00122-023-04490-z.ABSTRACTWheat, an important cereal crop globally, faces major challenges due to increasing global population and changing climates. The production and productivity are challenged by several biotic and abiotic stresses. There is also a pressing demand to enhance grain yield and quality/nutrition to ensure global food and nutritional security. To address these multifaceted concerns, researchers have conducted numerous meta-QTL (MQTL) studies in wheat, resulting in the identification of candidate genes that govern these complex quantitative traits. MQT...
Source: TAG. Theoretical and Applied Genetics - November 17, 2023 Category: Genetics & Stem Cells Authors: Divya Sharma Anita Kumari Priya Sharma Anupma Singh Anshu Sharma Zahoor Ahmad Mir Uttam Kumar Sofora Jan M Parthiban Reyazul Rouf Mir Pradeep Bhati Anjan Kumar Pradhan Aakash Yadav Dwijesh Chandra Mishra Neeraj Budhlakoti Mahesh C Yadav Kiran B Gaikwad Am Source Type: research

Fine mapping and candidate gene analysis of qSB12 < sup > YSB < /sup > , a gene conferring major quantitative resistance to rice sheath blight
In this study, we identified a QTL on chromosome 12, in which the qSB12YSB resistant allele shows significant ShB resistance, by using 150 BC4 backcross inbred lines employing the resistant rice variety YSBR1 as the donor and the susceptible variety Lemont (LE) as the recurrent parent. We further fine-mapped qSB12YSB to a 289-kb region by generating 34 chromosomal segment substitution lines and identified a total of 18 annotated genes as the most likely candidates for qSB12YSB after analyzing resequencing and transcriptomic data. KEGG analysis suggested that qSB12YSB might activate secondary metabolites biosynthesis and RO...
Source: TAG. Theoretical and Applied Genetics - November 16, 2023 Category: Genetics & Stem Cells Authors: Yu Wang Quanyi Sun Jianhua Zhao Taixuan Liu Haibo Du Wenfeng Shan Keting Wu Xiang Xue Chao Yang Jun Liu Zongxiang Chen Keming Hu Zhiming Feng Shimin Zuo Source Type: research

Genome-wide association study for temperature response and photo-thermal interaction of flowering time in soybean using a panel of cultivars with diverse maturity groups
Theor Appl Genet. 2023 Nov 14;136(12):245. doi: 10.1007/s00122-023-04496-7.ABSTRACTA total of 101 QTNs were found to be associated with soybean flowering time responses to photo-thermal conditions; three candidate genes with non-synonymous substitutions were identified: Glyma.08G302500 (GmHY5), Glyma.08G303900 (GmPIF4c), and Glyma.16G046700 (GmVRN1). The flowering transition is a crucial component of soybean (Glycine max L. Merr.) development. The transition process is regulated by photoperiod, temperature, and their interaction. To examine the genetic architecture associated with temperature- and photo-thermal-mediated re...
Source: TAG. Theoretical and Applied Genetics - November 14, 2023 Category: Genetics & Stem Cells Authors: Tingting Wu Huiwen Wen Xinyue Zhang Hongchang Jia Cailong Xu Wenwen Song Bingjun Jiang Shan Yuan Shi Sun Cunxiang Wu Tianfu Han Source Type: research