Correction to: In Situ Imaging of Virus-Infected Cells by Cryo-Electron Tomography: An Overview
Subcell Biochem. 2023;106:C1-C2. doi: 10.1007/978-3-031-40086-5_17.NO ABSTRACTPMID:38353937 | DOI:10.1007/978-3-031-40086-5_17 (Source: Sub-Cellular Biochemistry)
Source: Sub-Cellular Biochemistry - February 14, 2024 Category: Biochemistry Authors: Swetha Vijayakrishnan Source Type: research
Correction to: In Situ Imaging of Virus-Infected Cells by Cryo-Electron Tomography: An Overview
Subcell Biochem. 2023;106:C1-C2. doi: 10.1007/978-3-031-40086-5_17.NO ABSTRACTPMID:38353937 | DOI:10.1007/978-3-031-40086-5_17 (Source: Sub-Cellular Biochemistry)
Source: Sub-Cellular Biochemistry - February 14, 2024 Category: Biochemistry Authors: Swetha Vijayakrishnan Source Type: research
Correction to: In Situ Imaging of Virus-Infected Cells by Cryo-Electron Tomography: An Overview
Subcell Biochem. 2023;106:C1-C2. doi: 10.1007/978-3-031-40086-5_17.NO ABSTRACTPMID:38353937 | DOI:10.1007/978-3-031-40086-5_17 (Source: Sub-Cellular Biochemistry)
Source: Sub-Cellular Biochemistry - February 14, 2024 Category: Biochemistry Authors: Swetha Vijayakrishnan Source Type: research
Correction to: In Situ Imaging of Virus-Infected Cells by Cryo-Electron Tomography: An Overview
Subcell Biochem. 2023;106:C1-C2. doi: 10.1007/978-3-031-40086-5_17.NO ABSTRACTPMID:38353937 | DOI:10.1007/978-3-031-40086-5_17 (Source: Sub-Cellular Biochemistry)
Source: Sub-Cellular Biochemistry - February 14, 2024 Category: Biochemistry Authors: Swetha Vijayakrishnan Source Type: research
Correction to: In Situ Imaging of Virus-Infected Cells by Cryo-Electron Tomography: An Overview
Subcell Biochem. 2023;106:C1-C2. doi: 10.1007/978-3-031-40086-5_17.NO ABSTRACTPMID:38353937 | DOI:10.1007/978-3-031-40086-5_17 (Source: Sub-Cellular Biochemistry)
Source: Sub-Cellular Biochemistry - February 14, 2024 Category: Biochemistry Authors: Swetha Vijayakrishnan Source Type: research
Correction to: In Situ Imaging of Virus-Infected Cells by Cryo-Electron Tomography: An Overview
Subcell Biochem. 2023;106:C1-C2. doi: 10.1007/978-3-031-40086-5_17.NO ABSTRACTPMID:38353937 | DOI:10.1007/978-3-031-40086-5_17 (Source: Sub-Cellular Biochemistry)
Source: Sub-Cellular Biochemistry - February 14, 2024 Category: Biochemistry Authors: Swetha Vijayakrishnan Source Type: research
In Situ Imaging of Virus-Infected Cells by Cryo-Electron Tomography: An Overview
Subcell Biochem. 2023;106:3-36. doi: 10.1007/978-3-031-40086-5_1.ABSTRACTCryo-electron tomography (cryo-ET) has emerged as a powerful tool in structural biology to study viruses and is undergoing a resolution revolution. Enveloped viruses comprise several RNA and DNA pleomorphic viruses that are pathogens of clinical importance to humans and animals. Considerable efforts in cryogenic correlative light and electron microscopy (cryo-CLEM), cryogenic focused ion beam milling (cryo-FIB), and integrative structural techniques are helping to identify virus structures within cells leading to a rise of in situ discoveries shedding...
Source: Sub-Cellular Biochemistry - December 30, 2023 Category: Biochemistry Authors: Swetha Vijayakrishnan Source Type: research
Approaches to Evaluating Necroptosis in Virus-Infected Cells
Subcell Biochem. 2023;106:37-75. doi: 10.1007/978-3-031-40086-5_2.ABSTRACTThe immune system functions to protect the host from pathogens. To counter host defense mechanisms, pathogens have developed unique strategies to evade detection or restrict host immune responses. Programmed cell death is a major contributor to the multiple host responses that help to eliminate infected cells for obligate intracellular pathogens like viruses. Initiation of programmed cell death pathways during the early stages of viral infections is critical for organismal survival as it restricts the virus from replicating and serves to drive antivi...
Source: Sub-Cellular Biochemistry - December 30, 2023 Category: Biochemistry Authors: Crystal A Lawson Derek J Titus Heather S Koehler Source Type: research
Apoptosis and Phagocytosis as Antiviral Mechanisms
Subcell Biochem. 2023;106:77-112. doi: 10.1007/978-3-031-40086-5_3.ABSTRACTViruses are infectious entities that make use of the replication machinery of their hosts to produce more progenies, causing disease and sometimes death. To counter viral infection, metazoan hosts are equipped with various defense mechanisms, from the rapid-evoking innate immune responses to the most advanced adaptive immune responses. Previous research demonstrated that cells in fruit flies and mice infected with Drosophila C virus and influenza, respectively, undergo apoptosis, which triggers the engulfment of apoptotic virus-infected cells by pha...
Source: Sub-Cellular Biochemistry - December 30, 2023 Category: Biochemistry Authors: Firzan Nainu Youdiil Ophinni Akiko Shiratsuchi Yoshinobu Nakanishi Source Type: research
The Art of Viral Membrane Fusion and Penetration
Subcell Biochem. 2023;106:113-152. doi: 10.1007/978-3-031-40086-5_4.ABSTRACTAs obligate pathogens, viruses have developed diverse mechanisms to deliver their genome across host cell membranes to sites of virus replication. While enveloped viruses utilize viral fusion proteins to accomplish fusion of their envelope with the cellular membrane, non-enveloped viruses rely on machinery that causes local membrane ruptures and creates an opening through which the capsid or viral genome is released. Both membrane fusion and membrane penetration take place at the plasma membrane or in intracellular compartments, often involving the...
Source: Sub-Cellular Biochemistry - December 30, 2023 Category: Biochemistry Authors: Sophie L Winter Petr Chlanda Source Type: research
Single-Particle Tracking of Virus Entry in Live Cells
Subcell Biochem. 2023;106:153-168. doi: 10.1007/978-3-031-40086-5_5.ABSTRACTNovel imaging technologies such as single-particle tracking provide tools to study the intricate process of virus infection in host cells. In this chapter, we provide an overview of studies in which single-particle tracking technologies were applied for the analysis of the viral entry pathways in the context of the live host cell. Single-particle tracking techniques have been dependent on advances in the fluorescent labeling microscopy method and image analysis. The mechanistic and kinetic insights offered by this technique will provide a better un...
Source: Sub-Cellular Biochemistry - December 30, 2023 Category: Biochemistry Authors: Xiaowei Zhang Wei Li Zongqiang Cui Source Type: research
Correlative Cryo-imaging Using Soft X-Ray Tomography for the Study of Virus Biology in Cells and Tissues
Subcell Biochem. 2023;106:169-196. doi: 10.1007/978-3-031-40086-5_6.ABSTRACTViruses are obligate intracellular pathogens that depend on their host cell machinery and metabolism for their replicative life cycle. Virus entry, replication, and assembly are dynamic processes that lead to the reorganisation of host cell components. Therefore, a complete understanding of the viral processes requires their study in the cellular context where advanced imaging has been proven valuable in providing the necessary information. Among the available imaging techniques, soft X-ray tomography (SXT) at cryogenic temperatures can provide thr...
Source: Sub-Cellular Biochemistry - December 30, 2023 Category: Biochemistry Authors: Archana C Jadhav Ilias Kounatidis Source Type: research
The Virus-Induced Cytopathic Effect
Subcell Biochem. 2023;106:197-210. doi: 10.1007/978-3-031-40086-5_7.ABSTRACTThe cytopathic effect comprises the set of cellular alterations produced by a viral infection. It is of great relevance since it constitutes a direct marker of infection. Likewise, these alterations are often virus-specific which makes them a phenotypic marker for many viral species. All these characteristics have been used to complement the study of the dynamics of virus-cell interactions through the kinetic study of the progression of damage produced by the infection. Various approaches have been used to monitor the cytopathic effect, ranging fro...
Source: Sub-Cellular Biochemistry - December 30, 2023 Category: Biochemistry Authors: Daniel C éspedes-Tenorio Jorge L Arias-Arias Source Type: research
Human Papilloma Virus-Infected Cells
Subcell Biochem. 2023;106:213-226. doi: 10.1007/978-3-031-40086-5_8.ABSTRACTHuman papillomavirus (HPV) is associated with infection of different tissues, such as the cervix, anus, vagina, penis, vulva, oropharynx, throat, tonsils, back of the tongue, skin, the lungs, among other tissues. HPV infection may or may not be associated with the development of cancer, where HPVs not related to cancer are defined as low-risk HPVs and are associated with papillomatosis disease. In contrast, high-risk HPVs (HR-HPVs) are associated with developing cancers in areas that HR-HPV infects, such as the cervix. In general, infection of HPV ...
Source: Sub-Cellular Biochemistry - December 30, 2023 Category: Biochemistry Authors: Alfredo Cruz-Gregorio Ana Karina Aranda-Rivera Source Type: research
Defining the Assembleome of the Respiratory Syncytial Virus
Subcell Biochem. 2023;106:227-249. doi: 10.1007/978-3-031-40086-5_9.ABSTRACTDuring respiratory syncytial virus (RSV) particle assembly, the mature RSV particles form as filamentous projections on the surface of RSV-infected cells. The RSV assembly process occurs at the / on the cell surface that is modified by a virus infection, involving a combination of several different host cell factors and cellular processes. This induces changes in the lipid composition and properties of these lipid microdomains, and the virus-induced activation of associated Rho GTPase signaling networks drives the remodeling of the underlying filam...
Source: Sub-Cellular Biochemistry - December 30, 2023 Category: Biochemistry Authors: Richard J Sugrue Boon Huan Tan Source Type: research
