Molecular classification of glioblastoma based on immunohistochemical expression of EGFR, PDGFRA, NF1, IDH1, p53 and PTEN proteins
Pol J Pathol. 2021;72(1):1-10. doi: 10.5114/pjp.2021.106439.ABSTRACTGlioblastoma (GBM) is the most common and most aggressive primary tumor of the central nervous system. Current GBM treatments have low effectiveness. This is mainly due to the high degree of heterogeneity of GBM tumors. Despite similarities in the classic microscopic image, these tumors differ significantly in molecular terms. The aim of the study was to classify GBM tumors into one of four molecular types based on the immunohistochemical expression of EGFR, PDGFRA, NF1, IDH1, p53 and PTEN proteins and find the association between individual glioma molecul...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Sylwia Jankowska Magdalena Lewandowska Marta Masztalewicz Leszek Sagan Przemys ław Nowacki El żbieta Urasińska Source Type: research

The diagnostic utility of INSM1 and GATA3 in discriminating problematic medullary thyroid carcinoma, thyroid and parathyroid lesions
Pol J Pathol. 2021;72(1):11-22. doi: 10.5114/pjp.2021.106440.ABSTRACTDiscriminating thyroid and parathyroid lesions may sometimes pose a diagnostic difficulty. Medullary thyroid carcinomas (MTCs) display various cytologic and architectural features that resemble other thyroid and even rarely some parathyroid neoplasms. Moreover, some MTCs may have negative serum calcitonin, rendering them difficult to diagnose. Hence, to reach an appropriate diagnosis in problematic cases of these three categories - thyroid lesions, MTC and parathyroid lesions - the use of several immunohistochemical panels has been suggested and applied. ...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Sarah Adel Hakim Nermine Mohamed Abd Raboh Source Type: research

Prognostic and clinicopathological implications of expression of Beclin-1 and hypoxia-inducible factor 1 α in serous ovarian carcinoma: an immunohistochemical study
Pol J Pathol. 2021;72(1):23-38. doi: 10.5114/pjp.2021.106441.ABSTRACTSerous ovarian carcinoma (SOC) is an ovarian cancer with a high fatality rate. Therefore, a lot of researchers have tried to identify novel prognostic biomarkers which might improve the patient prognosis. The aims of the study were to detect the tissue protein expression of Beclin-1 in addition to HIF-1α in SOC patients, to evaluate the relationship between their expression, the clinicopathological parameters, patients' prognosis, and the relation to chemotherapy resistance in SOC. We evaluated the expression of Beclin-1 in addition to HIF-1α ...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Mohamed Alabiad Ola Harb Doaa Mandour Rehab Hemeda Rham Z Ahmed Ahmed El-Taher Gamal Osman Amany Shalaby Amr A A Alnemr Mostafa T Abdelfattah Source Type: research

Activation of the P38-MSK1 axis in colorectal adenocarcinoma determines a good prognostic outcome
In this study we evaluate expression of p-p38 and p-MSK1 in CRC and determine whether there is an association between expression of these markers and any clinicopathologic parameters that could be of prognostic value. Expression of p-p38, p-MSK1 and ki-67 were examined by immunohistochemistry in 135 archival CRC cases and the findings were correlated with the patient clinicopathological data. P-p38 and p-MSK1 were expressed at high level in 58.5 % and 60.7% of CRC cases respectively. A statistically significant negative correlation was found between expression of p-p38 and Ki-67 (p
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Mariana F Gayyed Medhat Monir Soliman Magdy Fouad Ahmed Taha Mohamed Hassanin Rajai Munir Al Jehani Fatma El Zahraa Ammar Mohamed Source Type: research

Immunohistochemical expression analysis of MMP-1, TIMP-2 and p53 in Barrett's esophagus, dysplasia and esophageal adenocarcinoma
Pol J Pathol. 2021;72(1):48-56. doi: 10.5114/pjp.2021.106443.ABSTRACTBarrett's esophagus (BE) is the most important risk factor for the development of esophageal adenocarcinoma. It develops through a progressive sequence of histologic and molecular events that begin with metaplasia and then progresses through various stages of dysplasia. Matrix metalloproteinases are involved in the degradation of the extracellular matrix and play an important role in tumor progression. The immunohistochemical expression of MMP-1, TIMP-2 and p53 in 111 samples from 45 patients diagnosed with BE with and without dysplasia and adenocarcinoma...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Alejandro Garc ía-Varona Iv án Fernández-Vega Jorge Santos-Juanes Source Type: research

Morphological features of non-parenchymal liver cells of full-term stillbirths from HIV-mono-infected mothers
Pol J Pathol. 2021;72(1):57-63. doi: 10.5114/pjp.2021.106444.ABSTRACTFifty-one liver tissue biopsies of full-term stillbirths from HIV-mono-infected mothers were investigated. All morphometric indices of non-parenchymal liver cells were calculated using the Avtandilov microscopic morphometric grid, which consisted of 100 equidistant points. It was inserted into the microscope ocular tube with a total × 200 microscope magnification. The number of points that were found in the corresponding types of non-parenchymal liver cells determined by immunohistochemistry was calculated. In each case, 10 random microscopic areas ...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Sergey Sherstiuk Stanislav Panov Igor Belozorov Tetiana Liadova Source Type: research

Assessment of HPV16 infection in patients with laryngeal cancer
Pol J Pathol. 2021;72(1):64-74. doi: 10.5114/pjp.2021.104659.ABSTRACTThe purpose of the study was to investigate HPV16 infection in laryngeal cancer patients treated with surgery and adjuvant radiotherapy as well as to analyze treatment results in relation to HPV16 infection and selected clinical, histopathological, and radiotherapy parameters. A retrospective analysis was performed in a group of 60 patients with squamous cell carcinoma of the larynx treated surgically and qualified for adjuvant radiotherapy at the Oncology Center in Cracow between 1995 and 2001. The studied group consisted of 57 men (95%) and 3 women (5%)...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Anna Mucha-Ma łecka Beata Biesaga Anna Janecka-Wid ła Marcin Przewo źnik Krzysztof Ma łecki Source Type: research

Interlaboratory agreement in assessment of gynaecological cytology in Cervical Cancer Screening Programme in Poland - a pilot evaluation
Pol J Pathol. 2021;72(1):75-83. doi: 10.5114/pjp.2021.106445.ABSTRACTIn our pilot study we have aimed to assess interlaboratory variability of cytological diagnoses in selected laboratories participating in the Polish Cervical Cancer Screening Programme (CCSP) to establish grounds for certification system for cytodiagnosticians and to monitor the quality of services. Set of 50 selected Pap smears, previously reassessed by an expert on the grounds of clinical, colposcopic and histological data was blinded and sent to 15 laboratories in Poland with request for evaluation according to routine practice according to the Bethesd...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Kinga Zalewska-Otwinowska Anna Macios Katarzyna Komerska Ma łgorzata Rekosz Andrzej Nowakowski Source Type: research

Endometrial adenocarcinoma with gastrointestinal differentiation - a newly described entity, with morphologic diversity
We present a case with morphologic diversity, with areas showing gastric and intestinal differentiation as well as an endometrioid-like component. However, the endometrioid-like component not only did not show classic squamous metaplasia, but was also MUC6-positive, while the positivity for ER/PR was only focal. The recognition of gastric/gastrointestinal differentiation in endometrial carcinomas is best accomplished using both morphology and immunohistochemistry rather than either alone.PMID:34060291 | DOI:10.5114/pjp.2021.106446 (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Simona Stolnicu Cristian Podoleanu Ildiko Orban Rozsnyai Francisc Source Type: research

Lymphocytic gastritis and celiac disease
Pol J Pathol. 2021;72(1):87-88. doi: 10.5114/pjp.2021.106447.ABSTRACTWe read with great interest the review article "Lymphocytic gastritis" by Puderecki et al., which was recently published in your journal [1]. The article describes the features of lymphocytic gastritis (LG), a rare form of gastritis with unclear pathogenesis. The diagnosis of LG is based on histology which reveals intraepithelial lymphocytosis (> 25 intraepithelial lymphocytes per 100 gastric surface and foveolar epithelial cells). The endoscopic appearance of LG can vary from normal mucosa to aphthous erosions, nodularity, local spots, polyp...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Angelo Viscido Giovanni Latella Source Type: research

Primary anaplastic large T-cell lymphoma of the psoas muscle
We describe a case of primary anaplastic large cell lymphoma (ALCL) arising from a deep skeletal muscle in a middle-aged male. He presented with a two-month history of swelling of his right thigh and mild fever, which led to a diagnosis of abscess formation. Antibiotics were prescribed for two weeks, with little improvement of symptoms. Subsequently, an exploratory surgery, with excision of the mass, demonstrated a ALCL of the psoas muscle, ALK-1 positive.PMID:34060293 | DOI:10.5114/pjp.2021.106448 (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Irene Pecorella Angela Ferrari Andrea Tornese Quirino Lai Gianluca Mennini Massimo Rossi Source Type: research

Molecular classification of glioblastoma based on immunohistochemical expression of EGFR, PDGFRA, NF1, IDH1, p53 and PTEN proteins
Pol J Pathol. 2021;72(1):1-10. doi: 10.5114/pjp.2021.106439.ABSTRACTGlioblastoma (GBM) is the most common and most aggressive primary tumor of the central nervous system. Current GBM treatments have low effectiveness. This is mainly due to the high degree of heterogeneity of GBM tumors. Despite similarities in the classic microscopic image, these tumors differ significantly in molecular terms. The aim of the study was to classify GBM tumors into one of four molecular types based on the immunohistochemical expression of EGFR, PDGFRA, NF1, IDH1, p53 and PTEN proteins and find the association between individual glioma molecul...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Sylwia Jankowska Magdalena Lewandowska Marta Masztalewicz Leszek Sagan Przemys ław Nowacki El żbieta Urasińska Source Type: research

The diagnostic utility of INSM1 and GATA3 in discriminating problematic medullary thyroid carcinoma, thyroid and parathyroid lesions
Pol J Pathol. 2021;72(1):11-22. doi: 10.5114/pjp.2021.106440.ABSTRACTDiscriminating thyroid and parathyroid lesions may sometimes pose a diagnostic difficulty. Medullary thyroid carcinomas (MTCs) display various cytologic and architectural features that resemble other thyroid and even rarely some parathyroid neoplasms. Moreover, some MTCs may have negative serum calcitonin, rendering them difficult to diagnose. Hence, to reach an appropriate diagnosis in problematic cases of these three categories - thyroid lesions, MTC and parathyroid lesions - the use of several immunohistochemical panels has been suggested and applied. ...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Sarah Adel Hakim Nermine Mohamed Abd Raboh Source Type: research

Prognostic and clinicopathological implications of expression of Beclin-1 and hypoxia-inducible factor 1 α in serous ovarian carcinoma: an immunohistochemical study
Pol J Pathol. 2021;72(1):23-38. doi: 10.5114/pjp.2021.106441.ABSTRACTSerous ovarian carcinoma (SOC) is an ovarian cancer with a high fatality rate. Therefore, a lot of researchers have tried to identify novel prognostic biomarkers which might improve the patient prognosis. The aims of the study were to detect the tissue protein expression of Beclin-1 in addition to HIF-1α in SOC patients, to evaluate the relationship between their expression, the clinicopathological parameters, patients' prognosis, and the relation to chemotherapy resistance in SOC. We evaluated the expression of Beclin-1 in addition to HIF-1α ...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Mohamed Alabiad Ola Harb Doaa Mandour Rehab Hemeda Rham Z Ahmed Ahmed El-Taher Gamal Osman Amany Shalaby Amr A A Alnemr Mostafa T Abdelfattah Source Type: research

Activation of the P38-MSK1 axis in colorectal adenocarcinoma determines a good prognostic outcome
In this study we evaluate expression of p-p38 and p-MSK1 in CRC and determine whether there is an association between expression of these markers and any clinicopathologic parameters that could be of prognostic value. Expression of p-p38, p-MSK1 and ki-67 were examined by immunohistochemistry in 135 archival CRC cases and the findings were correlated with the patient clinicopathological data. P-p38 and p-MSK1 were expressed at high level in 58.5 % and 60.7% of CRC cases respectively. A statistically significant negative correlation was found between expression of p-p38 and Ki-67 (p
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Mariana F Gayyed Medhat Monir Soliman Magdy Fouad Ahmed Taha Mohamed Hassanin Rajai Munir Al Jehani Fatma El Zahraa Ammar Mohamed Source Type: research

Immunohistochemical expression analysis of MMP-1, TIMP-2 and p53 in Barrett's esophagus, dysplasia and esophageal adenocarcinoma
Pol J Pathol. 2021;72(1):48-56. doi: 10.5114/pjp.2021.106443.ABSTRACTBarrett's esophagus (BE) is the most important risk factor for the development of esophageal adenocarcinoma. It develops through a progressive sequence of histologic and molecular events that begin with metaplasia and then progresses through various stages of dysplasia. Matrix metalloproteinases are involved in the degradation of the extracellular matrix and play an important role in tumor progression. The immunohistochemical expression of MMP-1, TIMP-2 and p53 in 111 samples from 45 patients diagnosed with BE with and without dysplasia and adenocarcinoma...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Alejandro Garc ía-Varona Iv án Fernández-Vega Jorge Santos-Juanes Source Type: research

Morphological features of non-parenchymal liver cells of full-term stillbirths from HIV-mono-infected mothers
Pol J Pathol. 2021;72(1):57-63. doi: 10.5114/pjp.2021.106444.ABSTRACTFifty-one liver tissue biopsies of full-term stillbirths from HIV-mono-infected mothers were investigated. All morphometric indices of non-parenchymal liver cells were calculated using the Avtandilov microscopic morphometric grid, which consisted of 100 equidistant points. It was inserted into the microscope ocular tube with a total × 200 microscope magnification. The number of points that were found in the corresponding types of non-parenchymal liver cells determined by immunohistochemistry was calculated. In each case, 10 random microscopic areas ...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Sergey Sherstiuk Stanislav Panov Igor Belozorov Tetiana Liadova Source Type: research

Assessment of HPV16 infection in patients with laryngeal cancer
Pol J Pathol. 2021;72(1):64-74. doi: 10.5114/pjp.2021.104659.ABSTRACTThe purpose of the study was to investigate HPV16 infection in laryngeal cancer patients treated with surgery and adjuvant radiotherapy as well as to analyze treatment results in relation to HPV16 infection and selected clinical, histopathological, and radiotherapy parameters. A retrospective analysis was performed in a group of 60 patients with squamous cell carcinoma of the larynx treated surgically and qualified for adjuvant radiotherapy at the Oncology Center in Cracow between 1995 and 2001. The studied group consisted of 57 men (95%) and 3 women (5%)...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Anna Mucha-Ma łecka Beata Biesaga Anna Janecka-Wid ła Marcin Przewo źnik Krzysztof Ma łecki Source Type: research

Interlaboratory agreement in assessment of gynaecological cytology in Cervical Cancer Screening Programme in Poland - a pilot evaluation
Pol J Pathol. 2021;72(1):75-83. doi: 10.5114/pjp.2021.106445.ABSTRACTIn our pilot study we have aimed to assess interlaboratory variability of cytological diagnoses in selected laboratories participating in the Polish Cervical Cancer Screening Programme (CCSP) to establish grounds for certification system for cytodiagnosticians and to monitor the quality of services. Set of 50 selected Pap smears, previously reassessed by an expert on the grounds of clinical, colposcopic and histological data was blinded and sent to 15 laboratories in Poland with request for evaluation according to routine practice according to the Bethesd...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Kinga Zalewska-Otwinowska Anna Macios Katarzyna Komerska Ma łgorzata Rekosz Andrzej Nowakowski Source Type: research

Endometrial adenocarcinoma with gastrointestinal differentiation - a newly described entity, with morphologic diversity
We present a case with morphologic diversity, with areas showing gastric and intestinal differentiation as well as an endometrioid-like component. However, the endometrioid-like component not only did not show classic squamous metaplasia, but was also MUC6-positive, while the positivity for ER/PR was only focal. The recognition of gastric/gastrointestinal differentiation in endometrial carcinomas is best accomplished using both morphology and immunohistochemistry rather than either alone.PMID:34060291 | DOI:10.5114/pjp.2021.106446 (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Simona Stolnicu Cristian Podoleanu Ildiko Orban Rozsnyai Francisc Source Type: research

Lymphocytic gastritis and celiac disease
Pol J Pathol. 2021;72(1):87-88. doi: 10.5114/pjp.2021.106447.ABSTRACTWe read with great interest the review article "Lymphocytic gastritis" by Puderecki et al., which was recently published in your journal [1]. The article describes the features of lymphocytic gastritis (LG), a rare form of gastritis with unclear pathogenesis. The diagnosis of LG is based on histology which reveals intraepithelial lymphocytosis (> 25 intraepithelial lymphocytes per 100 gastric surface and foveolar epithelial cells). The endoscopic appearance of LG can vary from normal mucosa to aphthous erosions, nodularity, local spots, polyp...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Angelo Viscido Giovanni Latella Source Type: research

Primary anaplastic large T-cell lymphoma of the psoas muscle
We describe a case of primary anaplastic large cell lymphoma (ALCL) arising from a deep skeletal muscle in a middle-aged male. He presented with a two-month history of swelling of his right thigh and mild fever, which led to a diagnosis of abscess formation. Antibiotics were prescribed for two weeks, with little improvement of symptoms. Subsequently, an exploratory surgery, with excision of the mass, demonstrated a ALCL of the psoas muscle, ALK-1 positive.PMID:34060293 | DOI:10.5114/pjp.2021.106448 (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Irene Pecorella Angela Ferrari Andrea Tornese Quirino Lai Gianluca Mennini Massimo Rossi Source Type: research

Molecular classification of glioblastoma based on immunohistochemical expression of EGFR, PDGFRA, NF1, IDH1, p53 and PTEN proteins
Pol J Pathol. 2021;72(1):1-10. doi: 10.5114/pjp.2021.106439.ABSTRACTGlioblastoma (GBM) is the most common and most aggressive primary tumor of the central nervous system. Current GBM treatments have low effectiveness. This is mainly due to the high degree of heterogeneity of GBM tumors. Despite similarities in the classic microscopic image, these tumors differ significantly in molecular terms. The aim of the study was to classify GBM tumors into one of four molecular types based on the immunohistochemical expression of EGFR, PDGFRA, NF1, IDH1, p53 and PTEN proteins and find the association between individual glioma molecul...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Sylwia Jankowska Magdalena Lewandowska Marta Masztalewicz Leszek Sagan Przemys ław Nowacki El żbieta Urasińska Source Type: research

The diagnostic utility of INSM1 and GATA3 in discriminating problematic medullary thyroid carcinoma, thyroid and parathyroid lesions
Pol J Pathol. 2021;72(1):11-22. doi: 10.5114/pjp.2021.106440.ABSTRACTDiscriminating thyroid and parathyroid lesions may sometimes pose a diagnostic difficulty. Medullary thyroid carcinomas (MTCs) display various cytologic and architectural features that resemble other thyroid and even rarely some parathyroid neoplasms. Moreover, some MTCs may have negative serum calcitonin, rendering them difficult to diagnose. Hence, to reach an appropriate diagnosis in problematic cases of these three categories - thyroid lesions, MTC and parathyroid lesions - the use of several immunohistochemical panels has been suggested and applied. ...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Sarah Adel Hakim Nermine Mohamed Abd Raboh Source Type: research

Prognostic and clinicopathological implications of expression of Beclin-1 and hypoxia-inducible factor 1 α in serous ovarian carcinoma: an immunohistochemical study
Pol J Pathol. 2021;72(1):23-38. doi: 10.5114/pjp.2021.106441.ABSTRACTSerous ovarian carcinoma (SOC) is an ovarian cancer with a high fatality rate. Therefore, a lot of researchers have tried to identify novel prognostic biomarkers which might improve the patient prognosis. The aims of the study were to detect the tissue protein expression of Beclin-1 in addition to HIF-1α in SOC patients, to evaluate the relationship between their expression, the clinicopathological parameters, patients' prognosis, and the relation to chemotherapy resistance in SOC. We evaluated the expression of Beclin-1 in addition to HIF-1α ...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Mohamed Alabiad Ola Harb Doaa Mandour Rehab Hemeda Rham Z Ahmed Ahmed El-Taher Gamal Osman Amany Shalaby Amr A A Alnemr Mostafa T Abdelfattah Source Type: research

Activation of the P38-MSK1 axis in colorectal adenocarcinoma determines a good prognostic outcome
In this study we evaluate expression of p-p38 and p-MSK1 in CRC and determine whether there is an association between expression of these markers and any clinicopathologic parameters that could be of prognostic value. Expression of p-p38, p-MSK1 and ki-67 were examined by immunohistochemistry in 135 archival CRC cases and the findings were correlated with the patient clinicopathological data. P-p38 and p-MSK1 were expressed at high level in 58.5 % and 60.7% of CRC cases respectively. A statistically significant negative correlation was found between expression of p-p38 and Ki-67 (p
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Mariana F Gayyed Medhat Monir Soliman Magdy Fouad Ahmed Taha Mohamed Hassanin Rajai Munir Al Jehani Fatma El Zahraa Ammar Mohamed Source Type: research

Immunohistochemical expression analysis of MMP-1, TIMP-2 and p53 in Barrett's esophagus, dysplasia and esophageal adenocarcinoma
Pol J Pathol. 2021;72(1):48-56. doi: 10.5114/pjp.2021.106443.ABSTRACTBarrett's esophagus (BE) is the most important risk factor for the development of esophageal adenocarcinoma. It develops through a progressive sequence of histologic and molecular events that begin with metaplasia and then progresses through various stages of dysplasia. Matrix metalloproteinases are involved in the degradation of the extracellular matrix and play an important role in tumor progression. The immunohistochemical expression of MMP-1, TIMP-2 and p53 in 111 samples from 45 patients diagnosed with BE with and without dysplasia and adenocarcinoma...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Alejandro Garc ía-Varona Iv án Fernández-Vega Jorge Santos-Juanes Source Type: research

Morphological features of non-parenchymal liver cells of full-term stillbirths from HIV-mono-infected mothers
Pol J Pathol. 2021;72(1):57-63. doi: 10.5114/pjp.2021.106444.ABSTRACTFifty-one liver tissue biopsies of full-term stillbirths from HIV-mono-infected mothers were investigated. All morphometric indices of non-parenchymal liver cells were calculated using the Avtandilov microscopic morphometric grid, which consisted of 100 equidistant points. It was inserted into the microscope ocular tube with a total × 200 microscope magnification. The number of points that were found in the corresponding types of non-parenchymal liver cells determined by immunohistochemistry was calculated. In each case, 10 random microscopic areas ...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Sergey Sherstiuk Stanislav Panov Igor Belozorov Tetiana Liadova Source Type: research

Assessment of HPV16 infection in patients with laryngeal cancer
Pol J Pathol. 2021;72(1):64-74. doi: 10.5114/pjp.2021.104659.ABSTRACTThe purpose of the study was to investigate HPV16 infection in laryngeal cancer patients treated with surgery and adjuvant radiotherapy as well as to analyze treatment results in relation to HPV16 infection and selected clinical, histopathological, and radiotherapy parameters. A retrospective analysis was performed in a group of 60 patients with squamous cell carcinoma of the larynx treated surgically and qualified for adjuvant radiotherapy at the Oncology Center in Cracow between 1995 and 2001. The studied group consisted of 57 men (95%) and 3 women (5%)...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Anna Mucha-Ma łecka Beata Biesaga Anna Janecka-Wid ła Marcin Przewo źnik Krzysztof Ma łecki Source Type: research

Interlaboratory agreement in assessment of gynaecological cytology in Cervical Cancer Screening Programme in Poland - a pilot evaluation
Pol J Pathol. 2021;72(1):75-83. doi: 10.5114/pjp.2021.106445.ABSTRACTIn our pilot study we have aimed to assess interlaboratory variability of cytological diagnoses in selected laboratories participating in the Polish Cervical Cancer Screening Programme (CCSP) to establish grounds for certification system for cytodiagnosticians and to monitor the quality of services. Set of 50 selected Pap smears, previously reassessed by an expert on the grounds of clinical, colposcopic and histological data was blinded and sent to 15 laboratories in Poland with request for evaluation according to routine practice according to the Bethesd...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Kinga Zalewska-Otwinowska Anna Macios Katarzyna Komerska Ma łgorzata Rekosz Andrzej Nowakowski Source Type: research

Endometrial adenocarcinoma with gastrointestinal differentiation - a newly described entity, with morphologic diversity
We present a case with morphologic diversity, with areas showing gastric and intestinal differentiation as well as an endometrioid-like component. However, the endometrioid-like component not only did not show classic squamous metaplasia, but was also MUC6-positive, while the positivity for ER/PR was only focal. The recognition of gastric/gastrointestinal differentiation in endometrial carcinomas is best accomplished using both morphology and immunohistochemistry rather than either alone.PMID:34060291 | DOI:10.5114/pjp.2021.106446 (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Simona Stolnicu Cristian Podoleanu Ildiko Orban Rozsnyai Francisc Source Type: research

Lymphocytic gastritis and celiac disease
Pol J Pathol. 2021;72(1):87-88. doi: 10.5114/pjp.2021.106447.ABSTRACTWe read with great interest the review article "Lymphocytic gastritis" by Puderecki et al., which was recently published in your journal [1]. The article describes the features of lymphocytic gastritis (LG), a rare form of gastritis with unclear pathogenesis. The diagnosis of LG is based on histology which reveals intraepithelial lymphocytosis (> 25 intraepithelial lymphocytes per 100 gastric surface and foveolar epithelial cells). The endoscopic appearance of LG can vary from normal mucosa to aphthous erosions, nodularity, local spots, polyp...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Angelo Viscido Giovanni Latella Source Type: research

Primary anaplastic large T-cell lymphoma of the psoas muscle
We describe a case of primary anaplastic large cell lymphoma (ALCL) arising from a deep skeletal muscle in a middle-aged male. He presented with a two-month history of swelling of his right thigh and mild fever, which led to a diagnosis of abscess formation. Antibiotics were prescribed for two weeks, with little improvement of symptoms. Subsequently, an exploratory surgery, with excision of the mass, demonstrated a ALCL of the psoas muscle, ALK-1 positive.PMID:34060293 | DOI:10.5114/pjp.2021.106448 (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Irene Pecorella Angela Ferrari Andrea Tornese Quirino Lai Gianluca Mennini Massimo Rossi Source Type: research

Molecular classification of glioblastoma based on immunohistochemical expression of EGFR, PDGFRA, NF1, IDH1, p53 and PTEN proteins
Pol J Pathol. 2021;72(1):1-10. doi: 10.5114/pjp.2021.106439.ABSTRACTGlioblastoma (GBM) is the most common and most aggressive primary tumor of the central nervous system. Current GBM treatments have low effectiveness. This is mainly due to the high degree of heterogeneity of GBM tumors. Despite similarities in the classic microscopic image, these tumors differ significantly in molecular terms. The aim of the study was to classify GBM tumors into one of four molecular types based on the immunohistochemical expression of EGFR, PDGFRA, NF1, IDH1, p53 and PTEN proteins and find the association between individual glioma molecul...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Sylwia Jankowska Magdalena Lewandowska Marta Masztalewicz Leszek Sagan Przemys ław Nowacki El żbieta Urasińska Source Type: research

The diagnostic utility of INSM1 and GATA3 in discriminating problematic medullary thyroid carcinoma, thyroid and parathyroid lesions
Pol J Pathol. 2021;72(1):11-22. doi: 10.5114/pjp.2021.106440.ABSTRACTDiscriminating thyroid and parathyroid lesions may sometimes pose a diagnostic difficulty. Medullary thyroid carcinomas (MTCs) display various cytologic and architectural features that resemble other thyroid and even rarely some parathyroid neoplasms. Moreover, some MTCs may have negative serum calcitonin, rendering them difficult to diagnose. Hence, to reach an appropriate diagnosis in problematic cases of these three categories - thyroid lesions, MTC and parathyroid lesions - the use of several immunohistochemical panels has been suggested and applied. ...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Sarah Adel Hakim Nermine Mohamed Abd Raboh Source Type: research

Prognostic and clinicopathological implications of expression of Beclin-1 and hypoxia-inducible factor 1 α in serous ovarian carcinoma: an immunohistochemical study
Pol J Pathol. 2021;72(1):23-38. doi: 10.5114/pjp.2021.106441.ABSTRACTSerous ovarian carcinoma (SOC) is an ovarian cancer with a high fatality rate. Therefore, a lot of researchers have tried to identify novel prognostic biomarkers which might improve the patient prognosis. The aims of the study were to detect the tissue protein expression of Beclin-1 in addition to HIF-1α in SOC patients, to evaluate the relationship between their expression, the clinicopathological parameters, patients' prognosis, and the relation to chemotherapy resistance in SOC. We evaluated the expression of Beclin-1 in addition to HIF-1α ...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Mohamed Alabiad Ola Harb Doaa Mandour Rehab Hemeda Rham Z Ahmed Ahmed El-Taher Gamal Osman Amany Shalaby Amr A A Alnemr Mostafa T Abdelfattah Source Type: research

Activation of the P38-MSK1 axis in colorectal adenocarcinoma determines a good prognostic outcome
In this study we evaluate expression of p-p38 and p-MSK1 in CRC and determine whether there is an association between expression of these markers and any clinicopathologic parameters that could be of prognostic value. Expression of p-p38, p-MSK1 and ki-67 were examined by immunohistochemistry in 135 archival CRC cases and the findings were correlated with the patient clinicopathological data. P-p38 and p-MSK1 were expressed at high level in 58.5 % and 60.7% of CRC cases respectively. A statistically significant negative correlation was found between expression of p-p38 and Ki-67 (p
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Mariana F Gayyed Medhat Monir Soliman Magdy Fouad Ahmed Taha Mohamed Hassanin Rajai Munir Al Jehani Fatma El Zahraa Ammar Mohamed Source Type: research

Immunohistochemical expression analysis of MMP-1, TIMP-2 and p53 in Barrett's esophagus, dysplasia and esophageal adenocarcinoma
Pol J Pathol. 2021;72(1):48-56. doi: 10.5114/pjp.2021.106443.ABSTRACTBarrett's esophagus (BE) is the most important risk factor for the development of esophageal adenocarcinoma. It develops through a progressive sequence of histologic and molecular events that begin with metaplasia and then progresses through various stages of dysplasia. Matrix metalloproteinases are involved in the degradation of the extracellular matrix and play an important role in tumor progression. The immunohistochemical expression of MMP-1, TIMP-2 and p53 in 111 samples from 45 patients diagnosed with BE with and without dysplasia and adenocarcinoma...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Alejandro Garc ía-Varona Iv án Fernández-Vega Jorge Santos-Juanes Source Type: research

Morphological features of non-parenchymal liver cells of full-term stillbirths from HIV-mono-infected mothers
Pol J Pathol. 2021;72(1):57-63. doi: 10.5114/pjp.2021.106444.ABSTRACTFifty-one liver tissue biopsies of full-term stillbirths from HIV-mono-infected mothers were investigated. All morphometric indices of non-parenchymal liver cells were calculated using the Avtandilov microscopic morphometric grid, which consisted of 100 equidistant points. It was inserted into the microscope ocular tube with a total × 200 microscope magnification. The number of points that were found in the corresponding types of non-parenchymal liver cells determined by immunohistochemistry was calculated. In each case, 10 random microscopic areas ...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Sergey Sherstiuk Stanislav Panov Igor Belozorov Tetiana Liadova Source Type: research

Assessment of HPV16 infection in patients with laryngeal cancer
Pol J Pathol. 2021;72(1):64-74. doi: 10.5114/pjp.2021.104659.ABSTRACTThe purpose of the study was to investigate HPV16 infection in laryngeal cancer patients treated with surgery and adjuvant radiotherapy as well as to analyze treatment results in relation to HPV16 infection and selected clinical, histopathological, and radiotherapy parameters. A retrospective analysis was performed in a group of 60 patients with squamous cell carcinoma of the larynx treated surgically and qualified for adjuvant radiotherapy at the Oncology Center in Cracow between 1995 and 2001. The studied group consisted of 57 men (95%) and 3 women (5%)...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Anna Mucha-Ma łecka Beata Biesaga Anna Janecka-Wid ła Marcin Przewo źnik Krzysztof Ma łecki Source Type: research

Interlaboratory agreement in assessment of gynaecological cytology in Cervical Cancer Screening Programme in Poland - a pilot evaluation
Pol J Pathol. 2021;72(1):75-83. doi: 10.5114/pjp.2021.106445.ABSTRACTIn our pilot study we have aimed to assess interlaboratory variability of cytological diagnoses in selected laboratories participating in the Polish Cervical Cancer Screening Programme (CCSP) to establish grounds for certification system for cytodiagnosticians and to monitor the quality of services. Set of 50 selected Pap smears, previously reassessed by an expert on the grounds of clinical, colposcopic and histological data was blinded and sent to 15 laboratories in Poland with request for evaluation according to routine practice according to the Bethesd...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Kinga Zalewska-Otwinowska Anna Macios Katarzyna Komerska Ma łgorzata Rekosz Andrzej Nowakowski Source Type: research

Endometrial adenocarcinoma with gastrointestinal differentiation - a newly described entity, with morphologic diversity
We present a case with morphologic diversity, with areas showing gastric and intestinal differentiation as well as an endometrioid-like component. However, the endometrioid-like component not only did not show classic squamous metaplasia, but was also MUC6-positive, while the positivity for ER/PR was only focal. The recognition of gastric/gastrointestinal differentiation in endometrial carcinomas is best accomplished using both morphology and immunohistochemistry rather than either alone.PMID:34060291 | DOI:10.5114/pjp.2021.106446 (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Simona Stolnicu Cristian Podoleanu Ildiko Orban Rozsnyai Francisc Source Type: research

Lymphocytic gastritis and celiac disease
Pol J Pathol. 2021;72(1):87-88. doi: 10.5114/pjp.2021.106447.ABSTRACTWe read with great interest the review article "Lymphocytic gastritis" by Puderecki et al., which was recently published in your journal [1]. The article describes the features of lymphocytic gastritis (LG), a rare form of gastritis with unclear pathogenesis. The diagnosis of LG is based on histology which reveals intraepithelial lymphocytosis (> 25 intraepithelial lymphocytes per 100 gastric surface and foveolar epithelial cells). The endoscopic appearance of LG can vary from normal mucosa to aphthous erosions, nodularity, local spots, polyp...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Angelo Viscido Giovanni Latella Source Type: research

Primary anaplastic large T-cell lymphoma of the psoas muscle
We describe a case of primary anaplastic large cell lymphoma (ALCL) arising from a deep skeletal muscle in a middle-aged male. He presented with a two-month history of swelling of his right thigh and mild fever, which led to a diagnosis of abscess formation. Antibiotics were prescribed for two weeks, with little improvement of symptoms. Subsequently, an exploratory surgery, with excision of the mass, demonstrated a ALCL of the psoas muscle, ALK-1 positive.PMID:34060293 | DOI:10.5114/pjp.2021.106448 (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Irene Pecorella Angela Ferrari Andrea Tornese Quirino Lai Gianluca Mennini Massimo Rossi Source Type: research

Molecular classification of glioblastoma based on immunohistochemical expression of EGFR, PDGFRA, NF1, IDH1, p53 and PTEN proteins
Pol J Pathol. 2021;72(1):1-10. doi: 10.5114/pjp.2021.106439.ABSTRACTGlioblastoma (GBM) is the most common and most aggressive primary tumor of the central nervous system. Current GBM treatments have low effectiveness. This is mainly due to the high degree of heterogeneity of GBM tumors. Despite similarities in the classic microscopic image, these tumors differ significantly in molecular terms. The aim of the study was to classify GBM tumors into one of four molecular types based on the immunohistochemical expression of EGFR, PDGFRA, NF1, IDH1, p53 and PTEN proteins and find the association between individual glioma molecul...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Sylwia Jankowska Magdalena Lewandowska Marta Masztalewicz Leszek Sagan Przemys ław Nowacki El żbieta Urasińska Source Type: research

The diagnostic utility of INSM1 and GATA3 in discriminating problematic medullary thyroid carcinoma, thyroid and parathyroid lesions
Pol J Pathol. 2021;72(1):11-22. doi: 10.5114/pjp.2021.106440.ABSTRACTDiscriminating thyroid and parathyroid lesions may sometimes pose a diagnostic difficulty. Medullary thyroid carcinomas (MTCs) display various cytologic and architectural features that resemble other thyroid and even rarely some parathyroid neoplasms. Moreover, some MTCs may have negative serum calcitonin, rendering them difficult to diagnose. Hence, to reach an appropriate diagnosis in problematic cases of these three categories - thyroid lesions, MTC and parathyroid lesions - the use of several immunohistochemical panels has been suggested and applied. ...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Sarah Adel Hakim Nermine Mohamed Abd Raboh Source Type: research

Prognostic and clinicopathological implications of expression of Beclin-1 and hypoxia-inducible factor 1 α in serous ovarian carcinoma: an immunohistochemical study
Pol J Pathol. 2021;72(1):23-38. doi: 10.5114/pjp.2021.106441.ABSTRACTSerous ovarian carcinoma (SOC) is an ovarian cancer with a high fatality rate. Therefore, a lot of researchers have tried to identify novel prognostic biomarkers which might improve the patient prognosis. The aims of the study were to detect the tissue protein expression of Beclin-1 in addition to HIF-1α in SOC patients, to evaluate the relationship between their expression, the clinicopathological parameters, patients' prognosis, and the relation to chemotherapy resistance in SOC. We evaluated the expression of Beclin-1 in addition to HIF-1α ...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Mohamed Alabiad Ola Harb Doaa Mandour Rehab Hemeda Rham Z Ahmed Ahmed El-Taher Gamal Osman Amany Shalaby Amr A A Alnemr Mostafa T Abdelfattah Source Type: research

Activation of the P38-MSK1 axis in colorectal adenocarcinoma determines a good prognostic outcome
In this study we evaluate expression of p-p38 and p-MSK1 in CRC and determine whether there is an association between expression of these markers and any clinicopathologic parameters that could be of prognostic value. Expression of p-p38, p-MSK1 and ki-67 were examined by immunohistochemistry in 135 archival CRC cases and the findings were correlated with the patient clinicopathological data. P-p38 and p-MSK1 were expressed at high level in 58.5 % and 60.7% of CRC cases respectively. A statistically significant negative correlation was found between expression of p-p38 and Ki-67 (p
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Mariana F Gayyed Medhat Monir Soliman Magdy Fouad Ahmed Taha Mohamed Hassanin Rajai Munir Al Jehani Fatma El Zahraa Ammar Mohamed Source Type: research

Immunohistochemical expression analysis of MMP-1, TIMP-2 and p53 in Barrett's esophagus, dysplasia and esophageal adenocarcinoma
Pol J Pathol. 2021;72(1):48-56. doi: 10.5114/pjp.2021.106443.ABSTRACTBarrett's esophagus (BE) is the most important risk factor for the development of esophageal adenocarcinoma. It develops through a progressive sequence of histologic and molecular events that begin with metaplasia and then progresses through various stages of dysplasia. Matrix metalloproteinases are involved in the degradation of the extracellular matrix and play an important role in tumor progression. The immunohistochemical expression of MMP-1, TIMP-2 and p53 in 111 samples from 45 patients diagnosed with BE with and without dysplasia and adenocarcinoma...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Alejandro Garc ía-Varona Iv án Fernández-Vega Jorge Santos-Juanes Source Type: research

Morphological features of non-parenchymal liver cells of full-term stillbirths from HIV-mono-infected mothers
Pol J Pathol. 2021;72(1):57-63. doi: 10.5114/pjp.2021.106444.ABSTRACTFifty-one liver tissue biopsies of full-term stillbirths from HIV-mono-infected mothers were investigated. All morphometric indices of non-parenchymal liver cells were calculated using the Avtandilov microscopic morphometric grid, which consisted of 100 equidistant points. It was inserted into the microscope ocular tube with a total × 200 microscope magnification. The number of points that were found in the corresponding types of non-parenchymal liver cells determined by immunohistochemistry was calculated. In each case, 10 random microscopic areas ...
Source: Polish Journal of Pathology - June 1, 2021 Category: Pathology Authors: Sergey Sherstiuk Stanislav Panov Igor Belozorov Tetiana Liadova Source Type: research