The prognostic relationship between histopathological and immunohistochemical features of hepatocellular carcinoma, intrahepatic cholangiocarcinoma and mixed type.
In this study, our first aim is to evaluate the relationship between prognosis and clinicopathological parameters. The second aim involves investigating the need for immunohistochemical staining and patterns of tumours to differentiate between them. Sixty-one cases were included in this study. For IHC, we used Hep par-1, CK7, CK19, CD56 and p53 staining, and the patterns of tumours were evaluated in haematoyxylin-eosin sections. No significant differences were found in Kaplan-Meier life analysis between the tumour types and OS and DFS values, but these values were greater in HCC than in I...
Source: Polish Journal of Pathology - August 1, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Assessment of CIP2A and ROCK-I expression and their prognostic value in breast cancer.
Authors: Tawab Osman N, Khalaf M, Ibraheem S Abstract Breast cancer is the most leading cause of cancer death in females worldwide. Identification of novel biomarkers for prognosis is required. Imunohistochemical evaluation of CIP2A and ROCK-1 expressions in 126 breast tissue specimens stratified as 21 ductal hyperplasias, 17 duct carcinoma in situ (DCIS) and 88 invasive carcinomas (56 invasive ductal carcinomas NST, 32 invasive lobular carcinomas) was studied. High CIP2A expression was detected in 48.9% of invasive carcinomas. CIP2A overexpression was significantly related to Nottingham pr...
Source: Polish Journal of Pathology - August 1, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Are there EWSR1 rearranged cutaneous hidradenomas and mucoepidermoid carcinomas of salivary glands? A FISH study and review of the literature.
In this study, EWSR1 and MAML2 translocation were investigated in hidradenomas and MECs. Specimens from thirteen MECs of the salivary glands and twenty hidradenomas of the skin were evaluated. Fluorescence in situ hybridisation (FISH) studies with EWSR1 and MAML2 break-apart probes were used. Forty percent of hidradenomas and 84.6% of MECs showed a positive MAML2 break signal. EWSR1 break signal was absent in hidradenomas. Only two MECs showed positive EWSR1 signal and were, thus, reclassified as clear cell carcinoma (CCC). A statistically significant relationship was also observed b...
Source: Polish Journal of Pathology - August 1, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

High Ki-67 expression is a marker of poor survival in apocrine breast carcinoma.
Authors: Wysocka J, Adamczyk A, Kruczak A, Niemiec J, Sas-Korczyńska B Abstract Apocrine carcinoma is a very rare type of breast cancer, which represents 0.5-4% of all breast cancers. The aim of the study was to analyze biological and clinical features of apocrine carcinoma and their influence on patients survival. The studied group consists of 57 patients, who underwent treatment between 1987 and 2010. Expression of ER, PgR, HER2, AR, GCDFP-15, EGFR, CK 5/6, CK 8/18 and Ki-67 was assessed immunohistochemically on formalin-fixed paraffin-embedded tissue sections. Presen...
Source: Polish Journal of Pathology - August 1, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

C-KIT mutation in thymic carcinomas.
We report a worse overall survival for patients with c-kit expressing tumours. These data suggest a negative prognostic role for c-kit expression especially within the first 5 years. PMID: 32729302 [PubMed - in process] (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - August 1, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

The importance of IDH1, ATRX and WT-1 mutations in glioblastoma.
In this study, we investigated the prognostic significance of IDH1 and ATRX mutations and WT-1 and p53 expression in glioblastomas and that of surgical methods, radiotherapy and chemotherapy. 83 patients with glioblastomas were retrospectively evaluated. Immunohistochemical analysis was performed for IDH1, ATRX and WT-1 expression. Tumour cells were positive for IDH1 in 9.6% of the patients. In 4.8% of the patients, loss of ATRX expression was observed in tumour cells; 86.7% of the patients were WT-1 positive, and 12.05% of the patients were p53 positive. No statistically significant difference was found in the progression...
Source: Polish Journal of Pathology - August 1, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Histopathological assessment of oral leukoplakia. Osteonectin as possible biomarker for further diagnostics.
Authors: Duś-Ilnicka I, Radwan-Oczko M, Gerber H, Hałoń A Abstract Clinical evaluation of oral leukoplakia (OL), confirmed by the histological evaluation of the suspected area, provides the gold standard for diagnostics of this pathology. The aim of present study was to encrypt the significance of the histopathological results (oral intraepithelial neoplasia - OIN, WHO 2005, Ljubljana classification systems) of OL. The usefulness of osteonectin as a biomarker of changes in the oral cavity epithelium was evaluated. IRS Score to evaluate osteonectin (SPARC - secreted protein acidic an...
Source: Polish Journal of Pathology - August 1, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Preparation of frozen cell-block sections amenableto diagnostic immunocytochemistry: a technical report on cryo-embedded cell-block method.
In this report, we demonstrate the amenability of F-CB sections to the diagnostic immunocytochemistry. The pelleted cytologic material was at first compactly embedded in ultralow-gelling temperature agarose gel and then re-embedded in conventional agarose gel. The resulting agarose-embedded cell-pellet was cut in halves so that one half is embedded in OCT compound for cryosectioning on a cryostat, while the other half is reserved for the preparation of paraffin-embedded CB (P-CB). The F-CB sections were comparable to sections cut from the paraffin-embedded CB in terms of the quality of H&E-staining and immunocytochemis...
Source: Polish Journal of Pathology - August 1, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Pan-p63 but not ΔNp63 (p40) expression in undifferentiated carcinoma of the pancreas.
Pan-p63 but not ΔNp63 (p40) expression in undifferentiated carcinoma of the pancreas. Pol J Pathol. 2020;71(2):155-172 Authors: Liszka Ł Abstract Undifferentiated carcinoma of the pancreas (UC) is a carcinoma without a definitive direction of differentiation. Tumour protein p63 is a regulator of squamous phenotype, which may also be engaged in tumour development. N-terminal isoforms of p63 are TAp63 and ΔNp63. Pan-p63 antibodies are able to detect both isoforms, whereas p40 antibodies recognise the ΔNp63 isoform only. The aim of the study was to describe pan-p63/p40 immunohistochemic...
Source: Polish Journal of Pathology - August 1, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

The haplotypes of L55M and Q192R PON1 polymorphisms and the risk of prostate cancer.
Authors: Saadat M PMID: 32729307 [PubMed - in process] (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - August 1, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Microscopic extraovarian sex cord proliferation: report of a case with bilateral Fallopian tube involvement and a comprehensive molecular analysis.
We report a case of a 58-year-old female with microscopic extraovarian sex cord proliferations affecting both Fallopian tubes. Molecular analysis showed likely pathogenic germline missense mutations of the KDM5A and KMT2D genes. However, mutations of other genes, including FOXL2 and STK11, were not detected. Our case represents the 12th case of extraovarian sex cord proliferation reported in the literature to date. This is the first time that a molecular genetic analysis of the lesion has been performed, and it showed a wild-type FOXL2 gene, which represents another argument supporting the estimated benign nature of these ...
Source: Polish Journal of Pathology - August 1, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Synovial sarcoma of the stomach: case report and systematic review of the literature.
We report a case of primary gastric synovial sarcoma in a 48-year-old female. Differential diagnosis of synovial sarcoma from other spindle cell, mesenchymal and cytokeratin-positive tumors is critical for the treatment and prognosis. Immunohistochemistry studies and molecular analysis are required to settle a proper diagnosis. PMID: 32729309 [PubMed - in process] (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - August 1, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Quiz. What is your diagnosis?
Authors: Licci S PMID: 32729310 [PubMed - in process] (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - August 1, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Nuclear CK19-immunopositive pseudoinclusions as a new additional objective diagnostic feature of papillary thyroid carcinoma.
Authors: Domagala P, Domagala W Abstract One of the key parameters in the diagnosis of papillary thyroid carcinoma (PTC) are true nuclear pseudoinclusions (NPs), which constitute invaginations of the cytoplasm into the nucleus. On the other hand, strong cytoplasmic expression of CK19 is a well-known attribute of PTC tumor cells. We analyzed NPs using CK19 immunohistochemistry in histological sections of 52 PTCs and seven noninvasive follicular thyroid neoplasms with papillary-like nuclear features (NIFTPs). Strong CK19+ NPs were present in 77% of PTCs...
Source: Polish Journal of Pathology - May 21, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Comparison of mutation profile between primary phyllodes tumors of the breast and their paired local recurrences.
Authors: Mitus J, Adamczyk A, Majchrzyk K, Kowalik A, Ryś J, Niemiec J Abstract Phyllodes tumor of the breast (PTB) is a rare neoplasm and accounts for 0.2-2.0% of breast cancer in women. Histopathological diagnosis of the tumor is difficult, and histological features do not always predict the course of the disease and the risk of progression. Pathogenesis and molecular biological characteristics as well as PTB prognostic factors are unknown. In search for genetic factors affecting PTB progression, 10 patients were analyzed for whom material from the primary tumor and local recurrence was available. DNA isolated f...
Source: Polish Journal of Pathology - May 21, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

The concordance of DNA mismatch repair protein between endoscopic biopsies and surgical specimens and inter-observers variations in colorectal cancer patients: reflections from endoscope doctors.
In this study, we aimed to investigate factors which are related to the consistence of MMRP evaluation between two pathologists or between different tissues. Two pathologists were asked to diagnose 4 MMRP, both on endoscopic biopsies and surgical materials, in 51 colorectal cancer (CRC) patients, using a single blind method. The consistence of two specimens and inter-observers' variances across different pathologists were compared respectively and the factors related to this variability were analyzed. Among the 816 paired MMRP, 804 (98.5%) pairs showed concordant IHC stains between biopsies and surgical materials, the agre...
Source: Polish Journal of Pathology - May 21, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Image analysis discloses differences in nuclear parameters between ERG+ and ERG- prostatic carcinomas.
Authors: Okoń K, Dyduch G, Białas MB, Milian-Ciesielska K, Szpor J, Leszczyńska I, Tyrak K, Szopiński T, Chłosta P Abstract Prostatic carcinoma (PC) is the most frequent urologic cancer and one of the most frequent cancers in males; it is a heterogeneous disease, in terms of molecular features, morphology and prognosis. About half of cases depends on TMPRSS2-ETS translocation which leads to a production of ERG transcription factor. ERG+ and ERG- cancers seem to differ in a number of features, which could lead to an altered nuclear structure; the aim of the study was to test this hypothesis. The material consis...
Source: Polish Journal of Pathology - May 21, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Evaluation of the expression of somatostatin receptors by immunohistochemistry in neuroendocrine tumors of the small intestine.
Authors: Abstract Most neuroendocrine neoplasms (NEN) are characterized by the presence of somatostatin receptors (SSTR) which we use in location diagnostics and treatment. The aim of this study was to evaluate the expression of somatostatin receptors by immunohistochemistry in tissue obtained after surgery of the primary focus in the small intestine. The group of patients consisted of 41 people, in 18 cases the primary tumor was in the jejunum and in 23 in the ileum. The immunohistochemical method was used to visualize the receptors, ...
Source: Polish Journal of Pathology - May 21, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Role of mast cells in the pathogenesis of liver fibrosis in nonalcoholic fatty liver disease.
Authors: Abstract Nonalcoholic fatty liver disease (NAFLD), a very common comorbidity of obesity, may progress from simple steatosis to nonalcoholic steatohepatitis and liver cirrhosis. The aim of this study was to determine whether mast cells (MCs) participate in the pathogenesis of liver fibrosis in patients with NAFLD. Liver specimens collected either from obese subjects or the control group were prepared for histological examination. The patients were divided into groups depending on inflammation and fibrosis grade, and the mean total number of mast cells/mm2 and those located only in portal areas/ fibrous sep...
Source: Polish Journal of Pathology - May 21, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

The level of myeloid derived-suppressor cells in peripheral blood of patients with prostate cancerafter various types of therapy.
In conclusion, we suggest that Mo-MDSC should be considered as a potential therapy target in the course of prostate cancer treatment to enhance its anti-tumor effectiveness. PMID: 32429654 [PubMed - in process] (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - May 21, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Clinical significance of PON1 L55M, Q192R and I102V polymorphisms and their association with prostate cancer risk in Polish men.
Authors: Abstract We tested the association between PON1 L55M, Q192R and I102V polymorphic variants and PC risk in Polish men. DNA from 110 consecutive, newly diagnosed patients hospitalized because of PC and DNA from 110 men - volunteers, healthy at the time of the study. PCR-RFLP method. In our study the average age at PC diagnosis of 55MM genotype carriers from families fulfilling Hereditary Prostate Criteria (HPC) was statistically significantly lower (by 7 years) than the average age at the disease diagnosis of 55MM carriers from families without HPC (54.6 ±6.7 vs. 61.9 ±5.4, respectively, p = 0...
Source: Polish Journal of Pathology - May 21, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Differences in GATA3 expression among histological/molecular subtypes and grades in infiltrating breast carcinoma (IBC) are important in the diagnosis of metastatic breast carcinoma.
Authors: Stolnicu S, Tunde C, Cadar A, Boros M Abstract GATA-binding protein 3 (GATA3) is a sensitive and relatively specific marker in invasive breast carcinomas (IBC). The aim of the present study was to determine GATA3 expression among different histological and surrogate molecular breast carcinoma subtypes. Immunohistochemical staining of ER, PR, Ki67, HER2 and GATA3 was performed in a cohort of 84 consecutive cases of primary IBCs. The association of GATA3 expression with different histological subtypes, grades and surrogate intrinsic molecular subtypes was assessed. The overall positivity of GATA3 across vari...
Source: Polish Journal of Pathology - May 21, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Liver involvement in NGLY1 congenital disorder of deglycosylation.
We reported on a patient in whom the liver biopsy done at the age of 3 years revealed the presence of steatosis, fibrosis, and an amorphous periodic acid-Schiff staining positive diastases-digested material in the cytoplasm. PMID: 32429657 [PubMed - in process] (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - May 21, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Analysis of mutations of PARP1, RNF213, PAX8, KMT2C, MTRR in malignant mesothelioma of testicular tunica vaginalis testis.
Authors: Kowalik A, Wincewicz A, Zięba S, Baran W, Kopczyński J, Koda M, Sulkowski S, Goźdź S Abstract Molecular next gene sequencing was used to evaluate mutations in 409 common mutated cancer-related genes in malignant mesothelioma of tunica vaginalis testis (MMTVT) of 81-year-old man. Multifocal papillary-solid areas contained necrosis among highly cellular fields with multiple mitoses. It was positive for WT1, CKAE1/AE3, calretinin, CK7 with negativity for CK5, PSA, TTF-1. Following mutations were revealed in PARP1 (NM_001618: c.2285TG, p.I49M) and two sorts of mutations in structure of KMT2C gene (NM_17060...
Source: Polish Journal of Pathology - May 21, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Quiz. What is your diagnosis?
Authors: Krupińska M, Kaznowska E, Kruczak A, Mularz K, Długosz J, Gancarz J, Ryś J PMID: 32429659 [PubMed - in process] (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - May 21, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Poorly differentiated clusters: prognostic significance in colorectal carcinomas immunohistochemistry images.
Authors: Jurescu A, Văduva A, Tăban S, Gheju A, Olteanu G, Mihai I, Lăzureanu C, Cornianu M, Lazăr F, Dema A Abstract Colorectal cancer (CRC) remains a major public health burden worldwide, despite increased knowledge on its pathogenesis and advances in therapy. We aimed to evaluate a new histological grading system based on poorly differentiated clusters (PDCs) counting - the PDCs grade (PDCs-G), and its clinicopathological and prognostic significance, compared to the World Health Organisation (WHO) grading system (WHO grade). We reviewed 71 surgical resection specimens for CRC from the Emergency County Hospit...
Source: Polish Journal of Pathology - March 12, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Molecular classification of diffuse gliomas.
In this study we assessed whether gliomas could be subdivided into different molecular subtypes by immunohistochemistry (IHC) reminiscent of those first described by Verhaak et al. in 2010 (classical, proneural, mesenchymal and neural). We also evaluated the prognostic significance of single molecular factors and searched for significant correlations between markers. In this study, we included 146 patients with glioblastomas (GBMs) and 26 with diffuse astrocytomas (DAs). The glioma samples were tested for PDGFRA, IDH1 R132H, CD44, p53, Ki-67, p21 and p27 expression. We found that gliomas could be subdivided into molecular ...
Source: Polish Journal of Pathology - March 12, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Clinical importance of fibulin-5 immunohistochemical staining in breast lesions.
Authors: Karanis M, Koksal H, Ates E, Dogru O Abstract The aim of this study was to evaluate the clinical importance of fibulin-5, which has been shown to display tumour-promoting and tumour-protective functions in breast lesions. Sixty-two breast cancer patients, 19 patients with fibroadenoma, and 15 healthy breast tissues were enrolled. Forty-seven patients had invasive ductal carcinoma (IDC) (12 of them also had in situ carcinoma DCIS), and 15 had invasive lobular carcinoma (ILC). A scoring system from 0 to 4 was used to evaluate the fibulin-5 staining according to the percentage of stained cells. The median val...
Source: Polish Journal of Pathology - March 12, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Fascin overexpression is associated with higher grades of breast cancer.
Authors: Abbasi A, Noroozinia F, Anvar S, Abbasi M, Hosseinzadeh S, Mokhtari S Abstract In the present study, we tried to evaluate the relationship between Fascin molecule expression and other histopathologic parameters, especially tumor grade and hormone profile. Sixty-one malignant breast tumors were enrolled in this study. Fascin marker staining was performed on paraffin blocks of breast samples using immunohistochemistry (IHC) method. The age range of patients was 26-87 years, with an average of 48.41 ±1.68. Our results showed that fascin expression was significantly higher in tumors with high grade hist...
Source: Polish Journal of Pathology - March 12, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Hepatocellular carcinoma - clinicopathological characteristics, survival, and expression of various histologic molecular markers.
Authors: Nikolova D, Ivanova V, Dimitrova M, Jovanovik R, Kunovska S, Orovcanec N, Petrushevska G, Janevska V Abstract The aim of this study was to make a clinical characterisation of patients with hepatocellular carcinoma and to investigate the expression of a set of molecular markers in patients from the Republic of North Macedonia. We analysed 60 patients for clinicopathologic factors, and we investigated tumour tissue and surrounding liver tissue for immunoexpression of E-cadherin, β-catenin, cyclin D1, and p53. Infection with hepatitis virus B and C (p
Source: Polish Journal of Pathology - March 12, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

The prognostic significance of tumour infiltrating lymphocytes in oral squamous cell carcinoma.
This study provides robust evidence that the density of tumour infiltrating lymphocytes demonstrates a high prognostic significance in oral squamous cell carcinoma. PMID: 32146797 [PubMed - in process] (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - March 12, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

CD56, CD57, HBME1, CK19, Galectin-3 and p63 immunohistochemical stains in differentiating diagnosis of thyroid benign/malign lesions and NIFTP.
In this study, we aimed to analyze the most commonly used immunohistochemical stains "HBME1, CK19, Galectin-3", adding the new ones "CD56, CD57, and p63". Study groups were; nodular hyperplasia, follicular adenoma, NIFTP, infiltrative follicular variant PTC, classical variant PTC (CVPTC) and follicular carcinoma. Each group consisted of twenty cases. The sections were stained with CD56, CD57, p63, CK19, HBME1 (Mesotel cell), Galectin-3 antibody. Although the expression of CD56 was high in benign follicular lesions, FC could not be excluded in this group. CD57 was high in malignant follicular group and N...
Source: Polish Journal of Pathology - March 12, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Green tea extract prevents the development of nonalcoholic liver steatosis in rats fed a high-fat diet.
Authors: Karolczak D, Seget M, Bajerska J, Błaszczyk A, Drzymała-Czyż S, Walkowiak J, Marszałek A Abstract Green tea contains many polyphenolic constitutes, which might prevent non-alcoholic fatty liver disease (NAFLD). We aimed to investigate whether green tea extract (GTE) given at doses reflecting habitual consumption of green tea beverages prevents development of NAFLD in rats fed a high-fat diet (HFD). Twenty-four male Wistar rats were randomly divided into four equal groups (two study and two control groups). The study groups received a HFD (approximately 50% energy from fat), enriched with 1.1% and 2.0% ...
Source: Polish Journal of Pathology - March 12, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Short-term prognosis of childhood hepatoblastoma in relation to ERCC1 C118T single nucleotide polymorphism and VEGF expression.
In this study, ERCC1 C118T SNP in children with HB was mainly found to be mutant type CT + TT. Compared to wild type CC, children with the mutant type CT + TT exhibited better treatment efficacy and remission with platinum-based chemotherapy as well as better survival rates. Moreover, the short-term prognosis of children with low VEGF expression was better than in those with high expression. PMID: 32146800 [PubMed - in process] (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - March 12, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Acquired von Willebrand syndrome associated with plasma cell myeloma - a rare histopathological bone marrow image.
We report a case of AVWS associated with plasma cell myeloma. The patient was a 57-year-old male with recurrent bleeding symptoms for a few months. Physical examination was normal. Laboratory studies revealed isolated prolongation of the activated partial thromboplastin time. His factor VIII activity, von Willebrand factor (VWF) Ag, and VWF activity were low. Bone marrow aspirate showed diffuse infiltration of atypical plasma cells and erythroid line hyperplasia. PMID: 32146801 [PubMed - in process] (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - March 12, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Pigmented/melanocytic malignant perivascular epithelioid cell tumor with TFE3-SFPQ(PSF) rearrangement - a challenging diagnosis of PEComa family of tumors.
Authors: Szumera-Cieækiewicz A, Kuczkiewicz-Siemion O, Seliga K, Grabowska-Kierył M, Tysarowski A, Wągrodzki M, Świtaj T, Prochorec-Sobieszek M, Rutkowski P Abstract We here report a case of a distinct subtype of pigmented/melanocytic malignant PEComa with TFE3-SFPQ(PSF) rearrangement. The tumor involved the iliac region and clinically mimicked metastatic melanoma. The immunohistochemical assessment was supplemented with molecular studies including fluorescence in situ hybridization (FISH) and next-generation sequencing sarcoma panel (NGS). We also discuss the differential diagnosis of intraabdominal PECom...
Source: Polish Journal of Pathology - March 12, 2020 Category: Pathology Tags: Pol J Pathol Source Type: research

Lymphocytic gastritis.
Authors: Puderecki M, Wronecki L, Cięszczyk K, Szumiło J Abstract Organic basis of gastrointestinal symptoms is in the scope of many specialists. In this article lymphocytic gastritis, relatively newly described and not widely-known entity is presented. The lesion is characterized by presence of numerous mature lymphocytes in the surface and foveolar epithelium, as well as lymphocytic infiltration of the lamina propria. According to the definition at least 25 lymphocytes per 100 gastric epithelial cells is now required for the diagnosis. Literature found in wide r...
Source: Polish Journal of Pathology - December 12, 2019 Category: Pathology Tags: Pol J Pathol Source Type: research

A robust and automated cell counting method in quantification of digital breast cancer immunohistochemistry images.
Authors: Chen L, Bao J, Huang Q, Sun H Abstract Quantitative analysis of immunohistochemically stained breast cancer specimens by cell counting is important for prognosis and treatment planning. This paper presents a robust, accurate, and novel method to label immunopositive and immunonegative cells automatically. During preprocessing, we developed an adaptive method to correct the colour aberration caused by imaging conditions. Next, a pixel-level segmentation was performed on preprocessed images using a support vector machine with a radial basis function kernel in HSV colour spa...
Source: Polish Journal of Pathology - December 12, 2019 Category: Pathology Tags: Pol J Pathol Source Type: research

Clinicopathologic characteristics of mixed epithelial/mesenchymal metaplastic breast carcinoma (carcinosarcoma): a meta-analysis of Chinese patients.
CONCLUSIONS: Breast carcinosarcoma is a rare subtype of metaplastic breast cancer. It is characterised by large tumour size, higher rates of axillary nodal involvement, higher rates of both local and distant recurrence, and is difficult to diagnose with preoperative core needle biopsies. Adjuvant treatment after surgical operation may improve the five-year OS of patients with breast carcinosarcoma. PMID: 31820860 [PubMed - in process] (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - December 12, 2019 Category: Pathology Tags: Pol J Pathol Source Type: research

The prognostic significance of CD63 expressionin patients with non-small cell lung cancer.
In this study, we aimed to investigate the prognostic significance of CD63 expression in non-small cell lung cancer (NSCLC). CD63 expression was evaluated in 133 cases of NSCLC via immunohistochemical staining using tissue microarray blocks. We assessed the relationship between CD63 expression and clinicopathological characteristics, as well as the prognostic significance of CD63 expression in NSCLC. CD63 expression was significantly correlated with patient gender (p 
Source: Polish Journal of Pathology - December 12, 2019 Category: Pathology Tags: Pol J Pathol Source Type: research

Low LATS2 expression is associated with poor prognosis in non-small cell lung carcinoma.
We examined LATS2 protein expression by immunohistochemistry in 184 resected NSCLC specimens using tissue microarrays. Low LATS2 expression was significantly related to disease recurrence (p = 0.047). In survival analysis, the low LATS2 expression group showed a statistically poorer overall survival (OS) (p = 0.004) and disease-free survival (DFS) (p = 0.014) than the high expression group. In multivariate analysis, downregulated LATS2 expression in NSCLC could be an independent prognostic factor of poor OS and DFS. Furthermore, we evaluated the prognostic signifi...
Source: Polish Journal of Pathology - December 12, 2019 Category: Pathology Tags: Pol J Pathol Source Type: research

Clinical significance of nuclear localisation of agrin in lung adenocarcinoma.
In this study, A total of 86 lung adenocarcinoma samples paired with adjacent non-tumour tissue samples and eight lung adenocarcinoma non-paired samples were selected for immunohistochemical staining for agrin. Strong staining of agrin in nuclei of lung adenocarcinoma tissues was observed, but not in the nuclei of normal lung tissues (p 
Source: Polish Journal of Pathology - December 12, 2019 Category: Pathology Tags: Pol J Pathol Source Type: research

Comparison of the ICC location in the gallbladder wall in patients with cholelithiasis and patients with non-calculous changes.
Authors: Spętana J, Lipiński A, Jeleń M Abstract Interstitial cells of Cajal (ICC) were first described by Santiago Ramon y Cajal over 100 years ago. They are thought to play an important role in the regulation of gastrointestinal motility. There is increasing evidence that the decline in their number in the gallbladder wall contributes to the formation of concrements. The aim of the study was to determine the exact location of interstitial cells of Cajal in the gallbladder wall in patients with calculous and non-calculous cholecystitis. ...
Source: Polish Journal of Pathology - December 12, 2019 Category: Pathology Tags: Pol J Pathol Source Type: research

Candida Albicans Alcohol Dehydrogenase 1 gene in oral dysplasia and oral squamous cell carcinoma.
This study was designed to examine the prevalence of Candida albicans alcohol dehydrogenase 1 (CaADH1) gene in oral dysplasia as well as oral squamous cell carcinoma (OSCC) with and without lymph node metastasis (LNM) using reverse transcription polymerase chain reaction (RT-PCR) in order to determine its role in initiation, propagation and metastasis of oral dysplasia and carcinoma. Formalin-fixed parafin-embedded specimens were grouped into four groups: 7 control, 16 oral dysplasia, 16 OSCC without LNM and 15 OSCC with LNM. All specimens were examined by periodic acid Schiff (PAS) stain to detect Candida h...
Source: Polish Journal of Pathology - December 12, 2019 Category: Pathology Tags: Pol J Pathol Source Type: research

The number of CD163 positive macrophages is associatedwith more advanced skin melanomas, microvessels density and patient prognosis.
Authors: Foks M, Wągrowska-Danilewicz M, Danilewicz M, Bonczysta M, Olborski B, Stasikowska-Kanicka O Abstract The study was aimed to evaluate the number of TAMs and to investigate whether they have association with microvessels density and patients' survival times. 46 cases of melanomas, divided into four groups according to the Breslow scale, were tested immunohistochemically with antibodies anti-CD68, CD163, iNOS to vizualized macrophages and anti-CD34 antibody to stain microvessels. The number of macrophages and the microvessels density were counted by hotspot analysis using ...
Source: Polish Journal of Pathology - December 12, 2019 Category: Pathology Tags: Pol J Pathol Source Type: research

TC2 C776G polymorphism and oral cancer.
Authors: Sriwijitalai W, Wiwanitkit V PMID: 31820867 [PubMed - in process] (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - December 12, 2019 Category: Pathology Tags: Pol J Pathol Source Type: research

Histopathological liver findings in patients with hepatocerebral mitochondrial depletion syndrome with defined molecular basis.
Authors: Pronicki M, Piekutowska-Abramczuk D, Rokicki D, Iwanicka-Pronicka K, Grajkowska W PMID: 31820868 [PubMed - in process] (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - December 12, 2019 Category: Pathology Tags: Pol J Pathol Source Type: research

Ovarian Sertoli-Leydig cell tumour with α-fetoprotein-producing intestinal glandular cells. Clinical case and short review of basic literature.
Ovarian Sertoli-Leydig cell tumour with α-fetoprotein-producing intestinal glandular cells. Clinical case and short review of basic literature. Pol J Pathol. 2019;70(3):226-231 Authors: Strus M, Rajtar-Ciosek A, Jach R, Hankus J, Szczepański W Abstract Sertoli Leydig cell tumor of the ovary, is a rare neoplasm from the group of sex cord-stromal tumors of the ovary, accounting for less than 1% of all ovarian tumors. Among the Sertoli Leydig cell tumors, we distinguish a separate group of tumors secreting α-fetoprotein (AFP). The young 24-year-old woman presented to the Clinical Department o...
Source: Polish Journal of Pathology - December 12, 2019 Category: Pathology Tags: Pol J Pathol Source Type: research

Quiz. What is your diagnosis?
Authors: Szumera-Ciećkiewicz A, Kuczkiewicz-Siemion O, Rutkowski P PMID: 31820870 [PubMed - in process] (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - December 12, 2019 Category: Pathology Tags: Pol J Pathol Source Type: research

Famous books in the history of pathology.
Authors: Tweel JGVD PMID: 31556544 [PubMed - in process] (Source: Polish Journal of Pathology)
Source: Polish Journal of Pathology - September 28, 2019 Category: Pathology Tags: Pol J Pathol Source Type: research