A simple air-liquid interface exposure system for exposing cultured human 3D epidermis and cornea to PM2.5 collected through cyclonic separation
In this study, we developed an air-liquid interface exposure system for exposing cultured human 3D epidermis and cornea to PM2.5 collected through cyclonic separation. PM2.5 suspension was nebulized in an acrylic chamber, and the resulting mist was pumped through a diffusion dryer into a glass exposure chamber. A particle counter was connected to the exposure chamber to continuously measure the spatial mass concentration of PM. Human 3D epidermis was cultured in the exposure chamber. Exposure of the human 3D epidermis to PM aerosol increased interleukin-8 release into the media around 50 µg/m3. Mass concentrations above 1...
Source: Journal of Toxicological Sciences - January 31, 2024 Category: Toxicology Authors: Maori Kono Masayuki Takaishi Tomoaki Okuda Masashi Fujihara Seisuke Noguchi Yasuhiro Ishihara Source Type: research
Valproic acid elevates HIF-1 α-mediated CGB expression and suppresses glucose uptake in BeWo cells
J Toxicol Sci. 2024;49(2):69-77. doi: 10.2131/jts.49.69.ABSTRACTPlacental dysfunction can disrupt pregnancy. However, few studies have assessed the effects of chemical-induced toxicity on placental function. Here, we examined the effects of valproic acid (VPA) as a model chemical on production of hormones and on glucose uptake in human choriocarcinoma cell line BeWo. Cells were treated with forskolin to differentiate into syncytiotrophoblasts, which were then treated with VPA for 72 hr. Real-time RT-PCR analysis showed that VPA significantly increased the mRNA expression of chorionic gonadotropin β (CGB), a hormone that i...
Source: Journal of Toxicological Sciences - January 31, 2024 Category: Toxicology Authors: Go Kitahara Kazuma Higashisaka Yurina Nakamoto Rena Yamamoto Wakako Okuno Momoe Serizawa Yuji Sakahashi Hirofumi Tsujino Yuya Haga Yasuo Tsutsumi Source Type: research
Drosophila melanogaster as potential alternative animal model for evaluating acute inhalation toxicity
In this study, we investigated the acute inhalation toxicity of methylisothiazolinone (MIT) and chloromethylisothiazolinone (CMIT) in a D. melanogaster model. During exposure, environmental conditions, mass median aerodynamic and geometric standard diameters were measured. After inhalation exposure, the survival rate, climbing ability, and bang sensitivity were measured on days 1, 2, and 7. Notably, the survival rate of flies decreased in an exposure concentration-dependent manner. Climbing ability and bang sensitivity were also altered in the MIT/CMIT group, compared with the negative control group. Overall, these results...
Source: Journal of Toxicological Sciences - January 31, 2024 Category: Toxicology Authors: Yoon Cho Chul Min Park Yong-Ju Heo Hae-Bin Park Min-Seok Kim Source Type: research
Methylmercury-induced brain neuronal death in CHOP-knockout mice
In this study, to clarify whether CHOP mediates MeHg-induced apoptosis, we examined the effect of CHOP deletion on MeHg exposure in CHOP-knockout mice. Our data showed that CHOP deletion had no effect on MeHg exposure-induced weight loss or hindlimb impairment in mice, nor did it increase apoptosis or inhibit neuronal cell loss. Hence, CHOP plays little role in MeHg toxicity, and other apoptotic pathways coupled with ER stress may be involved in MeHg-induced cell death.PMID:38296529 | DOI:10.2131/jts.49.55 (Source: Journal of Toxicological Sciences)
Source: Journal of Toxicological Sciences - January 31, 2024 Category: Toxicology Authors: Yuta Iijima Ryohei Miki Masatake Fujimura Seiichi Oyadomari Takashi Uehara Source Type: research
A simple air-liquid interface exposure system for exposing cultured human 3D epidermis and cornea to PM2.5 collected through cyclonic separation
In this study, we developed an air-liquid interface exposure system for exposing cultured human 3D epidermis and cornea to PM2.5 collected through cyclonic separation. PM2.5 suspension was nebulized in an acrylic chamber, and the resulting mist was pumped through a diffusion dryer into a glass exposure chamber. A particle counter was connected to the exposure chamber to continuously measure the spatial mass concentration of PM. Human 3D epidermis was cultured in the exposure chamber. Exposure of the human 3D epidermis to PM aerosol increased interleukin-8 release into the media around 50 µg/m3. Mass concentrations above 1...
Source: Journal of Toxicological Sciences - January 31, 2024 Category: Toxicology Authors: Maori Kono Masayuki Takaishi Tomoaki Okuda Masashi Fujihara Seisuke Noguchi Yasuhiro Ishihara Source Type: research
Valproic acid elevates HIF-1 α-mediated CGB expression and suppresses glucose uptake in BeWo cells
J Toxicol Sci. 2024;49(2):69-77. doi: 10.2131/jts.49.69.ABSTRACTPlacental dysfunction can disrupt pregnancy. However, few studies have assessed the effects of chemical-induced toxicity on placental function. Here, we examined the effects of valproic acid (VPA) as a model chemical on production of hormones and on glucose uptake in human choriocarcinoma cell line BeWo. Cells were treated with forskolin to differentiate into syncytiotrophoblasts, which were then treated with VPA for 72 hr. Real-time RT-PCR analysis showed that VPA significantly increased the mRNA expression of chorionic gonadotropin β (CGB), a hormone that i...
Source: Journal of Toxicological Sciences - January 31, 2024 Category: Toxicology Authors: Go Kitahara Kazuma Higashisaka Yurina Nakamoto Rena Yamamoto Wakako Okuno Momoe Serizawa Yuji Sakahashi Hirofumi Tsujino Yuya Haga Yasuo Tsutsumi Source Type: research
Drosophila melanogaster as potential alternative animal model for evaluating acute inhalation toxicity
In this study, we investigated the acute inhalation toxicity of methylisothiazolinone (MIT) and chloromethylisothiazolinone (CMIT) in a D. melanogaster model. During exposure, environmental conditions, mass median aerodynamic and geometric standard diameters were measured. After inhalation exposure, the survival rate, climbing ability, and bang sensitivity were measured on days 1, 2, and 7. Notably, the survival rate of flies decreased in an exposure concentration-dependent manner. Climbing ability and bang sensitivity were also altered in the MIT/CMIT group, compared with the negative control group. Overall, these results...
Source: Journal of Toxicological Sciences - January 31, 2024 Category: Toxicology Authors: Yoon Cho Chul Min Park Yong-Ju Heo Hae-Bin Park Min-Seok Kim Source Type: research
Methylmercury-induced brain neuronal death in CHOP-knockout mice
In this study, to clarify whether CHOP mediates MeHg-induced apoptosis, we examined the effect of CHOP deletion on MeHg exposure in CHOP-knockout mice. Our data showed that CHOP deletion had no effect on MeHg exposure-induced weight loss or hindlimb impairment in mice, nor did it increase apoptosis or inhibit neuronal cell loss. Hence, CHOP plays little role in MeHg toxicity, and other apoptotic pathways coupled with ER stress may be involved in MeHg-induced cell death.PMID:38296529 | DOI:10.2131/jts.49.55 (Source: Journal of Toxicological Sciences)
Source: Journal of Toxicological Sciences - January 31, 2024 Category: Toxicology Authors: Yuta Iijima Ryohei Miki Masatake Fujimura Seiichi Oyadomari Takashi Uehara Source Type: research
A simple air-liquid interface exposure system for exposing cultured human 3D epidermis and cornea to PM2.5 collected through cyclonic separation
In this study, we developed an air-liquid interface exposure system for exposing cultured human 3D epidermis and cornea to PM2.5 collected through cyclonic separation. PM2.5 suspension was nebulized in an acrylic chamber, and the resulting mist was pumped through a diffusion dryer into a glass exposure chamber. A particle counter was connected to the exposure chamber to continuously measure the spatial mass concentration of PM. Human 3D epidermis was cultured in the exposure chamber. Exposure of the human 3D epidermis to PM aerosol increased interleukin-8 release into the media around 50 µg/m3. Mass concentrations above 1...
Source: Journal of Toxicological Sciences - January 31, 2024 Category: Toxicology Authors: Maori Kono Masayuki Takaishi Tomoaki Okuda Masashi Fujihara Seisuke Noguchi Yasuhiro Ishihara Source Type: research
Valproic acid elevates HIF-1 α-mediated CGB expression and suppresses glucose uptake in BeWo cells
J Toxicol Sci. 2024;49(2):69-77. doi: 10.2131/jts.49.69.ABSTRACTPlacental dysfunction can disrupt pregnancy. However, few studies have assessed the effects of chemical-induced toxicity on placental function. Here, we examined the effects of valproic acid (VPA) as a model chemical on production of hormones and on glucose uptake in human choriocarcinoma cell line BeWo. Cells were treated with forskolin to differentiate into syncytiotrophoblasts, which were then treated with VPA for 72 hr. Real-time RT-PCR analysis showed that VPA significantly increased the mRNA expression of chorionic gonadotropin β (CGB), a hormone that i...
Source: Journal of Toxicological Sciences - January 31, 2024 Category: Toxicology Authors: Go Kitahara Kazuma Higashisaka Yurina Nakamoto Rena Yamamoto Wakako Okuno Momoe Serizawa Yuji Sakahashi Hirofumi Tsujino Yuya Haga Yasuo Tsutsumi Source Type: research
Drosophila melanogaster as potential alternative animal model for evaluating acute inhalation toxicity
In this study, we investigated the acute inhalation toxicity of methylisothiazolinone (MIT) and chloromethylisothiazolinone (CMIT) in a D. melanogaster model. During exposure, environmental conditions, mass median aerodynamic and geometric standard diameters were measured. After inhalation exposure, the survival rate, climbing ability, and bang sensitivity were measured on days 1, 2, and 7. Notably, the survival rate of flies decreased in an exposure concentration-dependent manner. Climbing ability and bang sensitivity were also altered in the MIT/CMIT group, compared with the negative control group. Overall, these results...
Source: Journal of Toxicological Sciences - January 31, 2024 Category: Toxicology Authors: Yoon Cho Chul Min Park Yong-Ju Heo Hae-Bin Park Min-Seok Kim Source Type: research
Methylmercury-induced brain neuronal death in CHOP-knockout mice
In this study, to clarify whether CHOP mediates MeHg-induced apoptosis, we examined the effect of CHOP deletion on MeHg exposure in CHOP-knockout mice. Our data showed that CHOP deletion had no effect on MeHg exposure-induced weight loss or hindlimb impairment in mice, nor did it increase apoptosis or inhibit neuronal cell loss. Hence, CHOP plays little role in MeHg toxicity, and other apoptotic pathways coupled with ER stress may be involved in MeHg-induced cell death.PMID:38296529 | DOI:10.2131/jts.49.55 (Source: Journal of Toxicological Sciences)
Source: Journal of Toxicological Sciences - January 31, 2024 Category: Toxicology Authors: Yuta Iijima Ryohei Miki Masatake Fujimura Seiichi Oyadomari Takashi Uehara Source Type: research
A simple air-liquid interface exposure system for exposing cultured human 3D epidermis and cornea to PM2.5 collected through cyclonic separation
In this study, we developed an air-liquid interface exposure system for exposing cultured human 3D epidermis and cornea to PM2.5 collected through cyclonic separation. PM2.5 suspension was nebulized in an acrylic chamber, and the resulting mist was pumped through a diffusion dryer into a glass exposure chamber. A particle counter was connected to the exposure chamber to continuously measure the spatial mass concentration of PM. Human 3D epidermis was cultured in the exposure chamber. Exposure of the human 3D epidermis to PM aerosol increased interleukin-8 release into the media around 50 µg/m3. Mass concentrations above 1...
Source: Journal of Toxicological Sciences - January 31, 2024 Category: Toxicology Authors: Maori Kono Masayuki Takaishi Tomoaki Okuda Masashi Fujihara Seisuke Noguchi Yasuhiro Ishihara Source Type: research
Valproic acid elevates HIF-1 α-mediated CGB expression and suppresses glucose uptake in BeWo cells
J Toxicol Sci. 2024;49(2):69-77. doi: 10.2131/jts.49.69.ABSTRACTPlacental dysfunction can disrupt pregnancy. However, few studies have assessed the effects of chemical-induced toxicity on placental function. Here, we examined the effects of valproic acid (VPA) as a model chemical on production of hormones and on glucose uptake in human choriocarcinoma cell line BeWo. Cells were treated with forskolin to differentiate into syncytiotrophoblasts, which were then treated with VPA for 72 hr. Real-time RT-PCR analysis showed that VPA significantly increased the mRNA expression of chorionic gonadotropin β (CGB), a hormone that i...
Source: Journal of Toxicological Sciences - January 31, 2024 Category: Toxicology Authors: Go Kitahara Kazuma Higashisaka Yurina Nakamoto Rena Yamamoto Wakako Okuno Momoe Serizawa Yuji Sakahashi Hirofumi Tsujino Yuya Haga Yasuo Tsutsumi Source Type: research
Drosophila melanogaster as potential alternative animal model for evaluating acute inhalation toxicity
In this study, we investigated the acute inhalation toxicity of methylisothiazolinone (MIT) and chloromethylisothiazolinone (CMIT) in a D. melanogaster model. During exposure, environmental conditions, mass median aerodynamic and geometric standard diameters were measured. After inhalation exposure, the survival rate, climbing ability, and bang sensitivity were measured on days 1, 2, and 7. Notably, the survival rate of flies decreased in an exposure concentration-dependent manner. Climbing ability and bang sensitivity were also altered in the MIT/CMIT group, compared with the negative control group. Overall, these results...
Source: Journal of Toxicological Sciences - January 31, 2024 Category: Toxicology Authors: Yoon Cho Chul Min Park Yong-Ju Heo Hae-Bin Park Min-Seok Kim Source Type: research
