Characters of the < em > MOCA < /em > family in wheat and < em > TaMOCA1 < /em > function in salt stress tolerance
In this study, we identified 110 MOCA genes in wheat which were classified into five clades and they differed in gene structure, protein length, conserved motifs and expression profiles in different tissues and under salt stress. TaMOCA1 was selected for further functional study in response to salt stress. TaMOCA1 was rapidly induced by NaCl treatment. The 35S::TaMOCA1-GFP construction showed the cell nucleus and cytoplasm location in wheat protoplast. TaMOCA1 over-expressing Arabidopsis seedlings formed longer primary roots and more lateral roots than the wild type ones under 50 mM NaCl treatment. The over-expressing Arab...
Source: Journal of Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Yuxiang Qin Ping Cui Bao Zhang Yuning Wang Source Type: research
Estimation of genetic diversity of the exotic Indian trout populations by using microsatellite markers
J Genet. 2024;103:11.ABSTRACTRainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta fario) are popular salmonid species that are reared for sport and recreational activities worldwide. In India, they were introduced and successfully established in the late 19th and early 20th centuries by the European settlers. However, until now, no studies have analysed the genetic integrity of wild trout populations in India. Therefore, this study aimed to analyse the genetic integrity of the wild rainbow trout populations from south India, one wild rainbow trout population from north India, and one wild brown trout populatio...
Source: Journal of Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Walter Devaa Vimal Panneerselvam Ramesh Uthandakalaipandian Source Type: research
Efficient assembly of a synthetic attenuated SARS-CoV-2 genome in < em > Saccharomyces cerevisiae < /em > using multi-copy yeast vectors
J Genet. 2024;103:09.ABSTRACTSaccharomyces cerevisiae has been demonstrated to be an excellent platform for the multi-fragment assembly of large DNA constructs through its powerful homologous recombination ability. These assemblies have invariably used the stable centromeric single copy vectors. However, many applications of these assembled genomes would benefit from assembly in a higher copy number vector for improved downstream extraction of intact genomes from the yeast. A review of the literature revealed that large multi-fragment assemblies did not appear to have been attempted in multicopy vectors. Therefore, we devi...
Source: Journal of Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Abhishek Kumar Singh Harsh Goar Nikita Vashist Prakash Sinha Anand Kumar Bachhawat Source Type: research
Characters of the < em > MOCA < /em > family in wheat and < em > TaMOCA1 < /em > function in salt stress tolerance
In this study, we identified 110 MOCA genes in wheat which were classified into five clades and they differed in gene structure, protein length, conserved motifs and expression profiles in different tissues and under salt stress. TaMOCA1 was selected for further functional study in response to salt stress. TaMOCA1 was rapidly induced by NaCl treatment. The 35S::TaMOCA1-GFP construction showed the cell nucleus and cytoplasm location in wheat protoplast. TaMOCA1 over-expressing Arabidopsis seedlings formed longer primary roots and more lateral roots than the wild type ones under 50 mM NaCl treatment. The over-expressing Arab...
Source: Journal of Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Yuxiang Qin Ping Cui Bao Zhang Yuning Wang Source Type: research
Estimation of genetic diversity of the exotic Indian trout populations by using microsatellite markers
J Genet. 2024;103:11.ABSTRACTRainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta fario) are popular salmonid species that are reared for sport and recreational activities worldwide. In India, they were introduced and successfully established in the late 19th and early 20th centuries by the European settlers. However, until now, no studies have analysed the genetic integrity of wild trout populations in India. Therefore, this study aimed to analyse the genetic integrity of the wild rainbow trout populations from south India, one wild rainbow trout population from north India, and one wild brown trout populatio...
Source: Journal of Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Walter Devaa Vimal Panneerselvam Ramesh Uthandakalaipandian Source Type: research
Efficient assembly of a synthetic attenuated SARS-CoV-2 genome in < em > Saccharomyces cerevisiae < /em > using multi-copy yeast vectors
J Genet. 2024;103:09.ABSTRACTSaccharomyces cerevisiae has been demonstrated to be an excellent platform for the multi-fragment assembly of large DNA constructs through its powerful homologous recombination ability. These assemblies have invariably used the stable centromeric single copy vectors. However, many applications of these assembled genomes would benefit from assembly in a higher copy number vector for improved downstream extraction of intact genomes from the yeast. A review of the literature revealed that large multi-fragment assemblies did not appear to have been attempted in multicopy vectors. Therefore, we devi...
Source: Journal of Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Abhishek Kumar Singh Harsh Goar Nikita Vashist Prakash Sinha Anand Kumar Bachhawat Source Type: research
Characters of the < em > MOCA < /em > family in wheat and < em > TaMOCA1 < /em > function in salt stress tolerance
In this study, we identified 110 MOCA genes in wheat which were classified into five clades and they differed in gene structure, protein length, conserved motifs and expression profiles in different tissues and under salt stress. TaMOCA1 was selected for further functional study in response to salt stress. TaMOCA1 was rapidly induced by NaCl treatment. The 35S::TaMOCA1-GFP construction showed the cell nucleus and cytoplasm location in wheat protoplast. TaMOCA1 over-expressing Arabidopsis seedlings formed longer primary roots and more lateral roots than the wild type ones under 50 mM NaCl treatment. The over-expressing Arab...
Source: Journal of Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Yuxiang Qin Ping Cui Bao Zhang Yuning Wang Source Type: research
Estimation of genetic diversity of the exotic Indian trout populations by using microsatellite markers
J Genet. 2024;103:11.ABSTRACTRainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta fario) are popular salmonid species that are reared for sport and recreational activities worldwide. In India, they were introduced and successfully established in the late 19th and early 20th centuries by the European settlers. However, until now, no studies have analysed the genetic integrity of wild trout populations in India. Therefore, this study aimed to analyse the genetic integrity of the wild rainbow trout populations from south India, one wild rainbow trout population from north India, and one wild brown trout populatio...
Source: Journal of Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Walter Devaa Vimal Panneerselvam Ramesh Uthandakalaipandian Source Type: research
Efficient assembly of a synthetic attenuated SARS-CoV-2 genome in < em > Saccharomyces cerevisiae < /em > using multi-copy yeast vectors
J Genet. 2024;103:09.ABSTRACTSaccharomyces cerevisiae has been demonstrated to be an excellent platform for the multi-fragment assembly of large DNA constructs through its powerful homologous recombination ability. These assemblies have invariably used the stable centromeric single copy vectors. However, many applications of these assembled genomes would benefit from assembly in a higher copy number vector for improved downstream extraction of intact genomes from the yeast. A review of the literature revealed that large multi-fragment assemblies did not appear to have been attempted in multicopy vectors. Therefore, we devi...
Source: Journal of Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Abhishek Kumar Singh Harsh Goar Nikita Vashist Prakash Sinha Anand Kumar Bachhawat Source Type: research
Characters of the < em > MOCA < /em > family in wheat and < em > TaMOCA1 < /em > function in salt stress tolerance
In this study, we identified 110 MOCA genes in wheat which were classified into five clades and they differed in gene structure, protein length, conserved motifs and expression profiles in different tissues and under salt stress. TaMOCA1 was selected for further functional study in response to salt stress. TaMOCA1 was rapidly induced by NaCl treatment. The 35S::TaMOCA1-GFP construction showed the cell nucleus and cytoplasm location in wheat protoplast. TaMOCA1 over-expressing Arabidopsis seedlings formed longer primary roots and more lateral roots than the wild type ones under 50 mM NaCl treatment. The over-expressing Arab...
Source: Journal of Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Yuxiang Qin Ping Cui Bao Zhang Yuning Wang Source Type: research
Estimation of genetic diversity of the exotic Indian trout populations by using microsatellite markers
J Genet. 2024;103:11.ABSTRACTRainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta fario) are popular salmonid species that are reared for sport and recreational activities worldwide. In India, they were introduced and successfully established in the late 19th and early 20th centuries by the European settlers. However, until now, no studies have analysed the genetic integrity of wild trout populations in India. Therefore, this study aimed to analyse the genetic integrity of the wild rainbow trout populations from south India, one wild rainbow trout population from north India, and one wild brown trout populatio...
Source: Journal of Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Walter Devaa Vimal Panneerselvam Ramesh Uthandakalaipandian Source Type: research
Efficient assembly of a synthetic attenuated SARS-CoV-2 genome in < em > Saccharomyces cerevisiae < /em > using multi-copy yeast vectors
J Genet. 2024;103:09.ABSTRACTSaccharomyces cerevisiae has been demonstrated to be an excellent platform for the multi-fragment assembly of large DNA constructs through its powerful homologous recombination ability. These assemblies have invariably used the stable centromeric single copy vectors. However, many applications of these assembled genomes would benefit from assembly in a higher copy number vector for improved downstream extraction of intact genomes from the yeast. A review of the literature revealed that large multi-fragment assemblies did not appear to have been attempted in multicopy vectors. Therefore, we devi...
Source: Journal of Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Abhishek Kumar Singh Harsh Goar Nikita Vashist Prakash Sinha Anand Kumar Bachhawat Source Type: research
Characters of the < em > MOCA < /em > family in wheat and < em > TaMOCA1 < /em > function in salt stress tolerance
In this study, we identified 110 MOCA genes in wheat which were classified into five clades and they differed in gene structure, protein length, conserved motifs and expression profiles in different tissues and under salt stress. TaMOCA1 was selected for further functional study in response to salt stress. TaMOCA1 was rapidly induced by NaCl treatment. The 35S::TaMOCA1-GFP construction showed the cell nucleus and cytoplasm location in wheat protoplast. TaMOCA1 over-expressing Arabidopsis seedlings formed longer primary roots and more lateral roots than the wild type ones under 50 mM NaCl treatment. The over-expressing Arab...
Source: Journal of Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Yuxiang Qin Ping Cui Bao Zhang Yuning Wang Source Type: research
Estimation of genetic diversity of the exotic Indian trout populations by using microsatellite markers
J Genet. 2024;103:11.ABSTRACTRainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta fario) are popular salmonid species that are reared for sport and recreational activities worldwide. In India, they were introduced and successfully established in the late 19th and early 20th centuries by the European settlers. However, until now, no studies have analysed the genetic integrity of wild trout populations in India. Therefore, this study aimed to analyse the genetic integrity of the wild rainbow trout populations from south India, one wild rainbow trout population from north India, and one wild brown trout populatio...
Source: Journal of Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Walter Devaa Vimal Panneerselvam Ramesh Uthandakalaipandian Source Type: research
Efficient assembly of a synthetic attenuated SARS-CoV-2 genome in < em > Saccharomyces cerevisiae < /em > using multi-copy yeast vectors
J Genet. 2024;103:09.ABSTRACTSaccharomyces cerevisiae has been demonstrated to be an excellent platform for the multi-fragment assembly of large DNA constructs through its powerful homologous recombination ability. These assemblies have invariably used the stable centromeric single copy vectors. However, many applications of these assembled genomes would benefit from assembly in a higher copy number vector for improved downstream extraction of intact genomes from the yeast. A review of the literature revealed that large multi-fragment assemblies did not appear to have been attempted in multicopy vectors. Therefore, we devi...
Source: Journal of Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Abhishek Kumar Singh Harsh Goar Nikita Vashist Prakash Sinha Anand Kumar Bachhawat Source Type: research
