The effect of modification of DNA interference on myostatin gene expression in mice
J Genet. 2024;103:02.ABSTRACTMyostatin is a known negative regulator of muscle tissue growth. Thus, an inhibitor of myostatin may be therapeutically useful as an anabolic agent for the muscle tissue. A promising gene-silencing approach for gene therapy is DNA interference (DNAi), a sequence that is complementary to the promoter region of a target gene. To confer resistance to nuclease digestion, several modifications such as methylphosphonate or phosphorothioate have been proposed, wherein a nonbridging oxygen atom in the oligonucleotide phosphate backbone is replaced by sulphur. The aim of the present study was to assess ...
Source: Journal of Genetics - January 8, 2024 Category: Genetics & Stem Cells Authors: Mitra Riasi Sina Mozaffari-Jovin Ali Javadmanesh Source Type: research
Fly clock, my clock, and lamin B receptor
J Genet. 2024;103:01.ABSTRACTIn the fruit fly Drosophila melanogaster, circadian rhythm was disrupted when the inner nuclear membrane protein lamin B receptor (LBR) was depleted from its clock neurons (Proc. Natl. Acad. Sci. USA 118, e2019756118. 2021; https://doi.org/10. 1073/pnas.2019756118 and Research 6, 0139, 2023; https://doi.org/10.34133/research.0139). Ordinarily, the clock proteinPERIOD (PER) forms foci close to the inner nuclear membrane in the circadian clock's repression phase. The size, number, and location of foci near the nuclear membrane oscillate with a 24-h rhythm. When LBR was absent the foci did not for...
Source: Journal of Genetics - January 8, 2024 Category: Genetics & Stem Cells Authors: Durgadas P Kasbekar Source Type: research
The effect of modification of DNA interference on myostatin gene expression in mice
J Genet. 2024;103:02.ABSTRACTMyostatin is a known negative regulator of muscle tissue growth. Thus, an inhibitor of myostatin may be therapeutically useful as an anabolic agent for the muscle tissue. A promising gene-silencing approach for gene therapy is DNA interference (DNAi), a sequence that is complementary to the promoter region of a target gene. To confer resistance to nuclease digestion, several modifications such as methylphosphonate or phosphorothioate have been proposed, wherein a nonbridging oxygen atom in the oligonucleotide phosphate backbone is replaced by sulphur. The aim of the present study was to assess ...
Source: Journal of Genetics - January 8, 2024 Category: Genetics & Stem Cells Authors: Mitra Riasi Sina Mozaffari-Jovin Ali Javadmanesh Source Type: research
Fly clock, my clock, and lamin B receptor
J Genet. 2024;103:01.ABSTRACTIn the fruit fly Drosophila melanogaster, circadian rhythm was disrupted when the inner nuclear membrane protein lamin B receptor (LBR) was depleted from its clock neurons (Proc. Natl. Acad. Sci. USA 118, e2019756118. 2021; https://doi.org/10. 1073/pnas.2019756118 and Research 6, 0139, 2023; https://doi.org/10.34133/research.0139). Ordinarily, the clock proteinPERIOD (PER) forms foci close to the inner nuclear membrane in the circadian clock's repression phase. The size, number, and location of foci near the nuclear membrane oscillate with a 24-h rhythm. When LBR was absent the foci did not for...
Source: Journal of Genetics - January 8, 2024 Category: Genetics & Stem Cells Authors: Durgadas P Kasbekar Source Type: research
The effect of modification of DNA interference on myostatin gene expression in mice
J Genet. 2024;103:02.ABSTRACTMyostatin is a known negative regulator of muscle tissue growth. Thus, an inhibitor of myostatin may be therapeutically useful as an anabolic agent for the muscle tissue. A promising gene-silencing approach for gene therapy is DNA interference (DNAi), a sequence that is complementary to the promoter region of a target gene. To confer resistance to nuclease digestion, several modifications such as methylphosphonate or phosphorothioate have been proposed, wherein a nonbridging oxygen atom in the oligonucleotide phosphate backbone is replaced by sulphur. The aim of the present study was to assess ...
Source: Journal of Genetics - January 8, 2024 Category: Genetics & Stem Cells Authors: Mitra Riasi Sina Mozaffari-Jovin Ali Javadmanesh Source Type: research
Exploring the influences of geographical variation on sequence signatures in the human gut microbiome
In this study, we have explored the available human gut microbiome 16S rRNA sequence datasets of cohorts representing large geographical gradients. The 16S rRNA sequences representing V3-V4 as well as V4 regions generated using Illumina sequencing chemistry in the MiSeq platform encompassing the United States of America, Chile, South Africa, Kuwait, and Malaysia were subjected to in-depth computational biology analyses. Firmicutes and Bacteroidetes were the most dominant phyla present in all studied cohorts but Actinobacteria was exclusively present in high abundance in cohorts from Malaysia (15.99%). The relative abundanc...
Source: Journal of Genetics - December 11, 2023 Category: Genetics & Stem Cells Authors: Gauraw Kumar Punyasloke Bhadury Source Type: research
< em > olf413 < /em > , a putative octopamine biosynthesis pathway gene is required for negative geotactic motor function in < em > Drosophila melanogaster < /em >
J Genet. 2023;102:52.ABSTRACTOlf413 gene annotated as CG12673 in the genome of Drosophila melanogaster has been predicted to code for a protein with putative function in octopamine biosynthesis. The expression pattern and the biological function of the gene awaits investigation. The present study is the first report where we describe its expression in the late pupal brain as depicted by enhancer-driven green fluorescent protein (GFP) expression. With experiments using loss of function olf413 mutant flies, we have demonstrated that olf413 function is essential for motor activity, gravity sensing and spatial balancing of the...
Source: Journal of Genetics - December 11, 2023 Category: Genetics & Stem Cells Authors: Ravindrakumar Ramya Venkatanarayanasetty Baragur Shyamala Source Type: research
Why are you hitting yourself? Whole-exome sequencing diagnosis of monogenic autoimmunity
J Genet. 2023;102:53.ABSTRACTInborn errors of immunity may present with autoimmunity and autoinflammation as hallmark clinical manifestations. We aimed to identify the potential monogenic causes of autoimmune disorders in 26 patients from a pediatric reference hospital in Mexico through whole-exome sequencing. We specifically selected patients with a family history of autoimmune diseases, early-onset symptoms, and difficult-to-control autoimmune disorders or autoimmunity associated with infection predisposition. We identified the genetic variants that were compatible with the patients' phenotype in 54% of the patients. Aut...
Source: Journal of Genetics - December 11, 2023 Category: Genetics & Stem Cells Authors: Lina M Castano-Jaramillo Francisco Rivas Larrauri Selma C Scheffler-Mendoza Alonso Gutierrez-Hernandez Juan Carlos Bustamante Ogando Paulina Colin Margarita Ortega Cisneros Sandra Rajme-L ópez Edgar Alejandro Medina-Torres Laura Berron Ruiz Ana Luisa Rod Source Type: research
Genetic diversity analysis of volunteer wheat based on SSR markers
J Genet. 2023;102:54.ABSTRACTVolunteer wheat is a kind of wheat with weed characteristics, distributed widely in the main wheat-producing areas of China. It seriously damages the yield and quality of cultivated wheat. To study the genetic diversity and population structure within and between volunteer wheat and cultivated wheat (Triticum aestivum L.), 195 volunteer wheat seeds and 29 cultivated wheat seeds were analysed based on 16 pairs of highly-polymorphic microsatellite simple sequence repeats (SSR) primers and a microchip capillary electrophoresis (MCE) detection system. A total of 110 polymorphic alleles were detecte...
Source: Journal of Genetics - December 11, 2023 Category: Genetics & Stem Cells Authors: Wangcang Su Hongle Xu Lanlan Sun Chuantao Lu Renhai Wu Source Type: research
Exploring the influences of geographical variation on sequence signatures in the human gut microbiome
In this study, we have explored the available human gut microbiome 16S rRNA sequence datasets of cohorts representing large geographical gradients. The 16S rRNA sequences representing V3-V4 as well as V4 regions generated using Illumina sequencing chemistry in the MiSeq platform encompassing the United States of America, Chile, South Africa, Kuwait, and Malaysia were subjected to in-depth computational biology analyses. Firmicutes and Bacteroidetes were the most dominant phyla present in all studied cohorts but Actinobacteria was exclusively present in high abundance in cohorts from Malaysia (15.99%). The relative abundanc...
Source: Journal of Genetics - December 11, 2023 Category: Genetics & Stem Cells Authors: Gauraw Kumar Punyasloke Bhadury Source Type: research
< em > olf413 < /em > , a putative octopamine biosynthesis pathway gene is required for negative geotactic motor function in < em > Drosophila melanogaster < /em >
J Genet. 2023;102:52.ABSTRACTOlf413 gene annotated as CG12673 in the genome of Drosophila melanogaster has been predicted to code for a protein with putative function in octopamine biosynthesis. The expression pattern and the biological function of the gene awaits investigation. The present study is the first report where we describe its expression in the late pupal brain as depicted by enhancer-driven green fluorescent protein (GFP) expression. With experiments using loss of function olf413 mutant flies, we have demonstrated that olf413 function is essential for motor activity, gravity sensing and spatial balancing of the...
Source: Journal of Genetics - December 11, 2023 Category: Genetics & Stem Cells Authors: Ravindrakumar Ramya Venkatanarayanasetty Baragur Shyamala Source Type: research
Why are you hitting yourself? Whole-exome sequencing diagnosis of monogenic autoimmunity
J Genet. 2023;102:53.ABSTRACTInborn errors of immunity may present with autoimmunity and autoinflammation as hallmark clinical manifestations. We aimed to identify the potential monogenic causes of autoimmune disorders in 26 patients from a pediatric reference hospital in Mexico through whole-exome sequencing. We specifically selected patients with a family history of autoimmune diseases, early-onset symptoms, and difficult-to-control autoimmune disorders or autoimmunity associated with infection predisposition. We identified the genetic variants that were compatible with the patients' phenotype in 54% of the patients. Aut...
Source: Journal of Genetics - December 11, 2023 Category: Genetics & Stem Cells Authors: Lina M Castano-Jaramillo Francisco Rivas Larrauri Selma C Scheffler-Mendoza Alonso Gutierrez-Hernandez Juan Carlos Bustamante Ogando Paulina Colin Margarita Ortega Cisneros Sandra Rajme-L ópez Edgar Alejandro Medina-Torres Laura Berron Ruiz Ana Luisa Rod Source Type: research
Genetic diversity analysis of volunteer wheat based on SSR markers
J Genet. 2023;102:54.ABSTRACTVolunteer wheat is a kind of wheat with weed characteristics, distributed widely in the main wheat-producing areas of China. It seriously damages the yield and quality of cultivated wheat. To study the genetic diversity and population structure within and between volunteer wheat and cultivated wheat (Triticum aestivum L.), 195 volunteer wheat seeds and 29 cultivated wheat seeds were analysed based on 16 pairs of highly-polymorphic microsatellite simple sequence repeats (SSR) primers and a microchip capillary electrophoresis (MCE) detection system. A total of 110 polymorphic alleles were detecte...
Source: Journal of Genetics - December 11, 2023 Category: Genetics & Stem Cells Authors: Wangcang Su Hongle Xu Lanlan Sun Chuantao Lu Renhai Wu Source Type: research
Exploring the influences of geographical variation on sequence signatures in the human gut microbiome
In this study, we have explored the available human gut microbiome 16S rRNA sequence datasets of cohorts representing large geographical gradients. The 16S rRNA sequences representing V3-V4 as well as V4 regions generated using Illumina sequencing chemistry in the MiSeq platform encompassing the United States of America, Chile, South Africa, Kuwait, and Malaysia were subjected to in-depth computational biology analyses. Firmicutes and Bacteroidetes were the most dominant phyla present in all studied cohorts but Actinobacteria was exclusively present in high abundance in cohorts from Malaysia (15.99%). The relative abundanc...
Source: Journal of Genetics - December 11, 2023 Category: Genetics & Stem Cells Authors: Gauraw Kumar Punyasloke Bhadury Source Type: research
< em > olf413 < /em > , a putative octopamine biosynthesis pathway gene is required for negative geotactic motor function in < em > Drosophila melanogaster < /em >
J Genet. 2023;102:52.ABSTRACTOlf413 gene annotated as CG12673 in the genome of Drosophila melanogaster has been predicted to code for a protein with putative function in octopamine biosynthesis. The expression pattern and the biological function of the gene awaits investigation. The present study is the first report where we describe its expression in the late pupal brain as depicted by enhancer-driven green fluorescent protein (GFP) expression. With experiments using loss of function olf413 mutant flies, we have demonstrated that olf413 function is essential for motor activity, gravity sensing and spatial balancing of the...
Source: Journal of Genetics - December 11, 2023 Category: Genetics & Stem Cells Authors: Ravindrakumar Ramya Venkatanarayanasetty Baragur Shyamala Source Type: research
