Journal of General and Applied Microbiology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Development of conjugation-mediated versatile site-specific single-copy luciferase fusion system
J Gen Appl Microbiol. 2023 Nov 7. doi: 10.2323/jgam.2023.10.001. Online ahead of print.ABSTRACTThere are a number of reporter systems that are useful for gene expression analysis in bacteria. However, at least in Salmonella, a versatile and simple luciferase reporter system that can be integrated precisely behind a promoter or gene of interest on a chromosome is not currently available. The luciferase operon luxCDABE from Photorhabdus luminescens has several advantages, including brightness, wide linear range, absence in most bacteria, stability at high temperature, and no substrate addition required for the assay. Here, a...
Source: Journal of General and Applied Microbiology - November 8, 2023 Category: Microbiology Authors: Akinori Kato Source Type: research

Heterologous expression and characterization of an M4 family extracellular metalloprotease for detergent application
J Gen Appl Microbiol. 2023 Oct 26. doi: 10.2323/jgam.2023.09.002. Online ahead of print.ABSTRACTProteolytic enzymes stand out as the most widely employed category utilized in manufacturing industry. A new protease was separated from Planococcus sp.11815 strain and named as nprS-15615 in this research. The gene of this protease has not been reported, and its enzymatic properties have been studied for the first time. To enhance enzyme production, the Planococcus sp. protease gene was expressed in Bacillus licheniformis 2709. The expression level of nprS-15615 was observed under the control of regulatory elements PaprE. nprS-...
Source: Journal of General and Applied Microbiology - October 25, 2023 Category: Microbiology Authors: Man Hao Chaoshuo Shi Weifeng Gong Jia Liu Xiangxin Meng Fufeng Liu Fuping Lu Huitu Zhang Source Type: research