Journal of General and Applied Microbiology 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Immobilization of fumarase from thermophilic eukaryotic red alga Cyanidioschyzon merolae on ceramic carrier
J Gen Appl Microbiol. 2024 Feb 29. doi: 10.2323/jgam.2024.02.003. Online ahead of print.ABSTRACTFumarase is an enzyme catalyzing reversible reaction between fumarate and L-malate in the citric acid cycle. Fumarase is used in the industrial production of L-malate, and its immobilization is required for reuse of the fumarases to reduce the cost. Accordingly, understanding the properties of immobilized fumarase is crucial, and several groups report on the storage stability and kinetic parameters of immobilized fumarase. Here we have immobilized fumarase from the thermophilic red alga Cyanidioschyzon merolae (CmFUM) on ceramic...
Source: Journal of General and Applied Microbiology - February 28, 2024 Category: Microbiology Authors: Miyo Yamane Kaori Iwazumi Takashi Osanai Source Type: research
Addition of α-1,3-glucan-binding domains to α-1,3-glucanase Agn1p from Schizosaccharomyces pombe enhances hydrolytic activity of insoluble α-1,3-glucan
This study involved genetically fusing TP, DS1, CBM6, TP, and DS2 to the C-terminus of Agn1p, generating the fusion enzyme Agn1p-DCD. The fusion enzyme was then expressed in Escherichia coli and purified from the cell-free extract. Agn1p-DCD and Agn1p exhibited similar characteristics, such as optimal pH, optimal temperature, pH stability, and thermostability. Insoluble α-1,3-glucan (1%) hydrolyzing assay showed that Agn1p-DCD and Agn1p released approximately 7.6 and 5.0 mM of reducing sugars, respectively, after 48 h of reaction. Kinetic analysis and an α-1,3-glucan binding assay indicated that the addition of DS1, CBM6...
Source: Journal of General and Applied Microbiology - February 12, 2024 Category: Microbiology Authors: Yui Horaguchi Moe Yokomichi Masaki Takahashi Fusheng Xu Hiroyuki Konno Koki Makabe Shigekazu Yano Source Type: research
Lactic acid fermentation of kamaboko, a heated Alaska pollock surimi, enhances angiotensin I-converting enzyme inhibitory activity via fish protein hydrolysis
J Gen Appl Microbiol. 2024 Jan 29. doi: 10.2323/jgam.2024.01.003. Online ahead of print.ABSTRACTTo enhance the value of surimi, efforts have been made to develop a fermentation method with lactic acid bacteria (LAB) to proteolyze fish protein. However, fermenting unheated surimi poses a spoilage risk due to its high bacterial content. Surimi heat treatment can prevent spoilage, but gel formation induced by heating introduces another technical issue: it hinders uniform fermentation. Thus, this study aims to observe the proteolysis and enhance the functionality of seafood product through lactic acid fermentation of kamaboko,...
Source: Journal of General and Applied Microbiology - January 28, 2024 Category: Microbiology Authors: Kazuya Kobayashi Natsuka Takada Yuki Matsubara Hiroaki Okuhara Masaki Oosaka Source Type: research
Systematic promoter design for plasmid-encoded S-adenosylmethionine sensing systems
J Gen Appl Microbiol. 2024 Jan 29. doi: 10.2323/jgam.2024.01.002. Online ahead of print.ABSTRACTS-adenosylmethionine (SAM) is an important biomolecule that mainly acts as a methyl donor and plays many roles in a variety of biological functions. SAM is also required for the biosynthesis of valuable methylated compounds, but its supply is a bottleneck for these biosynthetic pathways. To overcome this bottleneck and to reconfigure SAM homeostasis, a high-throughput sensing system for changes in intracellular SAM availability is required. We constructed a plasmid that can detect the factors that can alter SAM availability usin...
Source: Journal of General and Applied Microbiology - January 28, 2024 Category: Microbiology Authors: Taro Watanabe Yuki Kimura Daisuke Umeno Source Type: research
CRISPRi knockdown of the cyabrB1 gene induces the divergently transcribed icfG and sll1783 operons related to carbon metabolism in the cyanobacterium Synechocystis sp. PCC 6803
In this study, we investigated the function of the essential gene cyabrB1 (sll0359) in Synechocystis sp. PCC 6803 by using CRISPR interference technology. The conditional knockdown of cyabrB1 caused increases of cyAbrB2 transcript and protein levels. However, the effect of cyabrB1 knockdown on global gene expression profile was quite limited compared to the previously reported profound effect of knockout of cyabrB2. Among 24 up-regulated genes, 16 genes were members of the divergently transcribed icfG and sll1783 operons related to carbon metabolism. The results of this and previous studies indicate the different contribut...
Source: Journal of General and Applied Microbiology - January 24, 2024 Category: Microbiology Authors: Atsuko Hishida Ryo Shirai Akiyoshi Higo Minenosuke Matsutani Kaori Nimura-Matsune Tomoko Takahashi Satoru Watanabe Shigeki Ehira Yukako Hihara Source Type: research
Identification of the Csr global regulatory system mediated by small RNA decay in Aeromonas salmonicida
This study underscores the conservation and functionality of the Csr system and raises questions about its regulatory targets and mechanisms in A. salmonicida.PMID:38233172 | DOI:10.2323/jgam.2023.12.004 (Source: Journal of General and Applied Microbiology)
Source: Journal of General and Applied Microbiology - January 17, 2024 Category: Microbiology Authors: Olga Gladyshchuk Masaki Yoshida Koume Togashi Hayuki Sugimoto Kazushi Suzuki Source Type: research
