Effects of S-adenosylmethionine on AfsKRS regulation in pristinamycin biosynthesis in Streptomyces pristinaespiralis
J Gen Appl Microbiol. 2024 Apr 5. doi: 10.2323/jgam.2024.03.002. Online ahead of print.ABSTRACTIn Streptomyces pristinaespiralis, AfsKRS system has differential regulation for PI and PII component biosynthesis of pristinamycin, but it is unknown whether S-adenosylmethionine (SAM) plays an important role in the AfsK-AfsR-AfsS signal transduction cascade during pristinamycin production. The possible target of exogenous SAM in the AfsKRS system and the biological role of SAM during the production of PI and PII were investigated using three mutantsΔafsK,ΔafsR andΔafsS defective in signal cascade pathway of AfsKRS. It was fo...
Source: Journal of General and Applied Microbiology - April 7, 2024 Category: Microbiology Authors: Yan Liu Na Shen Zhige Wu Yu Yang Xinyan Dong Zhihua Jin Qingchao Jin Source Type: research

Cellulolytic enzymes in Microbulbifer sp. Strain GL-2, a marine fish intestinal bacterium, with emphasis on endo-1,4- β-glucanases Cel5A and Cel8
J Gen Appl Microbiol. 2024 Mar 27. doi: 10.2323/jgam.2024.03.001. Online ahead of print.ABSTRACTCellulose is an abundant biomass on the planet. Various cellulases from environmental microbes have been explored for industrial use of cellulose. Marine fish intestine is of interest as one source of new enzymes. Here, we report the discovery of genes encoding two β-glucosidases (Bgl3A and Bgl3B) and four endo-1,4-β-glucanases (Cel5A, Cel8, Cel5B, and Cel9) as part of the genome sequence of a cellulolytic marine bacterium, Microbulbifer sp. Strain GL-2. Five of these six enzymes (excepting Cel5B) are presumed to localize to t...
Source: Journal of General and Applied Microbiology - March 27, 2024 Category: Microbiology Authors: Ken-Ichiro Ohnishi Seiya Watanabe Aya Kadoya Satoru Suzuki Source Type: research