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Immobilization of fumarase from thermophilic eukaryotic red alga Cyanidioschyzon merolae on ceramic carrier
J Gen Appl Microbiol. 2024 Feb 29. doi: 10.2323/jgam.2024.02.003. Online ahead of print.ABSTRACTFumarase is an enzyme catalyzing reversible reaction between fumarate and L-malate in the citric acid cycle. Fumarase is used in the industrial production of L-malate, and its immobilization is required for reuse of the fumarases to reduce the cost. Accordingly, understanding the properties of immobilized fumarase is crucial, and several groups report on the storage stability and kinetic parameters of immobilized fumarase. Here we have immobilized fumarase from the thermophilic red alga Cyanidioschyzon merolae (CmFUM) on ceramic...
Source: Journal of General and Applied Microbiology - February 28, 2024 Category: Microbiology Authors: Miyo Yamane Kaori Iwazumi Takashi Osanai Source Type: research

Isolation of hitherto-uncultivated microorganisms- Application of radical scavengers
J Gen Appl Microbiol. 2024 Feb 28. doi: 10.2323/jgam.2024.02.002. Online ahead of print.ABSTRACTThe culture filtrates of the predominant bacterial strains isolated from soil samples have been shown to increase the microbial colony counts on agar plates used for the isolation of uncultured bacteria. One of the factors in the culture filtrates responsible for this increase was identified to be superoxide dismutase (SOD). The generation of reactive oxygen species (O2-, H2O2, and ・OH) was detected from conventional laboratory agar media. The use of agar media supplemented with radical scavengers (SOD, catalase, ascorbic acid...
Source: Journal of General and Applied Microbiology - February 28, 2024 Category: Microbiology Authors: Yoko Takahashi Source Type: research

Immobilization of fumarase from thermophilic eukaryotic red alga Cyanidioschyzon merolae on ceramic carrier
J Gen Appl Microbiol. 2024 Feb 29. doi: 10.2323/jgam.2024.02.003. Online ahead of print.ABSTRACTFumarase is an enzyme catalyzing reversible reaction between fumarate and L-malate in the citric acid cycle. Fumarase is used in the industrial production of L-malate, and its immobilization is required for reuse of the fumarases to reduce the cost. Accordingly, understanding the properties of immobilized fumarase is crucial, and several groups report on the storage stability and kinetic parameters of immobilized fumarase. Here we have immobilized fumarase from the thermophilic red alga Cyanidioschyzon merolae (CmFUM) on ceramic...
Source: Journal of General and Applied Microbiology - February 28, 2024 Category: Microbiology Authors: Miyo Yamane Kaori Iwazumi Takashi Osanai Source Type: research

Isolation of hitherto-uncultivated microorganisms- Application of radical scavengers
J Gen Appl Microbiol. 2024 Feb 28. doi: 10.2323/jgam.2024.02.002. Online ahead of print.ABSTRACTThe culture filtrates of the predominant bacterial strains isolated from soil samples have been shown to increase the microbial colony counts on agar plates used for the isolation of uncultured bacteria. One of the factors in the culture filtrates responsible for this increase was identified to be superoxide dismutase (SOD). The generation of reactive oxygen species (O2-, H2O2, and ・OH) was detected from conventional laboratory agar media. The use of agar media supplemented with radical scavengers (SOD, catalase, ascorbic acid...
Source: Journal of General and Applied Microbiology - February 28, 2024 Category: Microbiology Authors: Yoko Takahashi Source Type: research

Immobilization of fumarase from thermophilic eukaryotic red alga Cyanidioschyzon merolae on ceramic carrier
J Gen Appl Microbiol. 2024 Feb 29. doi: 10.2323/jgam.2024.02.003. Online ahead of print.ABSTRACTFumarase is an enzyme catalyzing reversible reaction between fumarate and L-malate in the citric acid cycle. Fumarase is used in the industrial production of L-malate, and its immobilization is required for reuse of the fumarases to reduce the cost. Accordingly, understanding the properties of immobilized fumarase is crucial, and several groups report on the storage stability and kinetic parameters of immobilized fumarase. Here we have immobilized fumarase from the thermophilic red alga Cyanidioschyzon merolae (CmFUM) on ceramic...
Source: Journal of General and Applied Microbiology - February 28, 2024 Category: Microbiology Authors: Miyo Yamane Kaori Iwazumi Takashi Osanai Source Type: research

Isolation of hitherto-uncultivated microorganisms- Application of radical scavengers
J Gen Appl Microbiol. 2024 Feb 28. doi: 10.2323/jgam.2024.02.002. Online ahead of print.ABSTRACTThe culture filtrates of the predominant bacterial strains isolated from soil samples have been shown to increase the microbial colony counts on agar plates used for the isolation of uncultured bacteria. One of the factors in the culture filtrates responsible for this increase was identified to be superoxide dismutase (SOD). The generation of reactive oxygen species (O2-, H2O2, and ・OH) was detected from conventional laboratory agar media. The use of agar media supplemented with radical scavengers (SOD, catalase, ascorbic acid...
Source: Journal of General and Applied Microbiology - February 28, 2024 Category: Microbiology Authors: Yoko Takahashi Source Type: research

Immobilization of fumarase from thermophilic eukaryotic red alga Cyanidioschyzon merolae on ceramic carrier
J Gen Appl Microbiol. 2024 Feb 29. doi: 10.2323/jgam.2024.02.003. Online ahead of print.ABSTRACTFumarase is an enzyme catalyzing reversible reaction between fumarate and L-malate in the citric acid cycle. Fumarase is used in the industrial production of L-malate, and its immobilization is required for reuse of the fumarases to reduce the cost. Accordingly, understanding the properties of immobilized fumarase is crucial, and several groups report on the storage stability and kinetic parameters of immobilized fumarase. Here we have immobilized fumarase from the thermophilic red alga Cyanidioschyzon merolae (CmFUM) on ceramic...
Source: Journal of General and Applied Microbiology - February 28, 2024 Category: Microbiology Authors: Miyo Yamane Kaori Iwazumi Takashi Osanai Source Type: research

Isolation of hitherto-uncultivated microorganisms- Application of radical scavengers
J Gen Appl Microbiol. 2024 Feb 28. doi: 10.2323/jgam.2024.02.002. Online ahead of print.ABSTRACTThe culture filtrates of the predominant bacterial strains isolated from soil samples have been shown to increase the microbial colony counts on agar plates used for the isolation of uncultured bacteria. One of the factors in the culture filtrates responsible for this increase was identified to be superoxide dismutase (SOD). The generation of reactive oxygen species (O2-, H2O2, and ・OH) was detected from conventional laboratory agar media. The use of agar media supplemented with radical scavengers (SOD, catalase, ascorbic acid...
Source: Journal of General and Applied Microbiology - February 28, 2024 Category: Microbiology Authors: Yoko Takahashi Source Type: research

Immobilization of fumarase from thermophilic eukaryotic red alga Cyanidioschyzon merolae on ceramic carrier
J Gen Appl Microbiol. 2024 Feb 29. doi: 10.2323/jgam.2024.02.003. Online ahead of print.ABSTRACTFumarase is an enzyme catalyzing reversible reaction between fumarate and L-malate in the citric acid cycle. Fumarase is used in the industrial production of L-malate, and its immobilization is required for reuse of the fumarases to reduce the cost. Accordingly, understanding the properties of immobilized fumarase is crucial, and several groups report on the storage stability and kinetic parameters of immobilized fumarase. Here we have immobilized fumarase from the thermophilic red alga Cyanidioschyzon merolae (CmFUM) on ceramic...
Source: Journal of General and Applied Microbiology - February 28, 2024 Category: Microbiology Authors: Miyo Yamane Kaori Iwazumi Takashi Osanai Source Type: research

Isolation of hitherto-uncultivated microorganisms- Application of radical scavengers
J Gen Appl Microbiol. 2024 Feb 28. doi: 10.2323/jgam.2024.02.002. Online ahead of print.ABSTRACTThe culture filtrates of the predominant bacterial strains isolated from soil samples have been shown to increase the microbial colony counts on agar plates used for the isolation of uncultured bacteria. One of the factors in the culture filtrates responsible for this increase was identified to be superoxide dismutase (SOD). The generation of reactive oxygen species (O2-, H2O2, and ・OH) was detected from conventional laboratory agar media. The use of agar media supplemented with radical scavengers (SOD, catalase, ascorbic acid...
Source: Journal of General and Applied Microbiology - February 28, 2024 Category: Microbiology Authors: Yoko Takahashi Source Type: research

Immobilization of fumarase from thermophilic eukaryotic red alga Cyanidioschyzon merolae on ceramic carrier
J Gen Appl Microbiol. 2024 Feb 29. doi: 10.2323/jgam.2024.02.003. Online ahead of print.ABSTRACTFumarase is an enzyme catalyzing reversible reaction between fumarate and L-malate in the citric acid cycle. Fumarase is used in the industrial production of L-malate, and its immobilization is required for reuse of the fumarases to reduce the cost. Accordingly, understanding the properties of immobilized fumarase is crucial, and several groups report on the storage stability and kinetic parameters of immobilized fumarase. Here we have immobilized fumarase from the thermophilic red alga Cyanidioschyzon merolae (CmFUM) on ceramic...
Source: Journal of General and Applied Microbiology - February 28, 2024 Category: Microbiology Authors: Miyo Yamane Kaori Iwazumi Takashi Osanai Source Type: research

Isolation of hitherto-uncultivated microorganisms- Application of radical scavengers
J Gen Appl Microbiol. 2024 Feb 28. doi: 10.2323/jgam.2024.02.002. Online ahead of print.ABSTRACTThe culture filtrates of the predominant bacterial strains isolated from soil samples have been shown to increase the microbial colony counts on agar plates used for the isolation of uncultured bacteria. One of the factors in the culture filtrates responsible for this increase was identified to be superoxide dismutase (SOD). The generation of reactive oxygen species (O2-, H2O2, and ・OH) was detected from conventional laboratory agar media. The use of agar media supplemented with radical scavengers (SOD, catalase, ascorbic acid...
Source: Journal of General and Applied Microbiology - February 28, 2024 Category: Microbiology Authors: Yoko Takahashi Source Type: research

Immobilization of fumarase from thermophilic eukaryotic red alga Cyanidioschyzon merolae on ceramic carrier
J Gen Appl Microbiol. 2024 Feb 29. doi: 10.2323/jgam.2024.02.003. Online ahead of print.ABSTRACTFumarase is an enzyme catalyzing reversible reaction between fumarate and L-malate in the citric acid cycle. Fumarase is used in the industrial production of L-malate, and its immobilization is required for reuse of the fumarases to reduce the cost. Accordingly, understanding the properties of immobilized fumarase is crucial, and several groups report on the storage stability and kinetic parameters of immobilized fumarase. Here we have immobilized fumarase from the thermophilic red alga Cyanidioschyzon merolae (CmFUM) on ceramic...
Source: Journal of General and Applied Microbiology - February 28, 2024 Category: Microbiology Authors: Miyo Yamane Kaori Iwazumi Takashi Osanai Source Type: research

Isolation of hitherto-uncultivated microorganisms- Application of radical scavengers
J Gen Appl Microbiol. 2024 Feb 28. doi: 10.2323/jgam.2024.02.002. Online ahead of print.ABSTRACTThe culture filtrates of the predominant bacterial strains isolated from soil samples have been shown to increase the microbial colony counts on agar plates used for the isolation of uncultured bacteria. One of the factors in the culture filtrates responsible for this increase was identified to be superoxide dismutase (SOD). The generation of reactive oxygen species (O2-, H2O2, and ・OH) was detected from conventional laboratory agar media. The use of agar media supplemented with radical scavengers (SOD, catalase, ascorbic acid...
Source: Journal of General and Applied Microbiology - February 28, 2024 Category: Microbiology Authors: Yoko Takahashi Source Type: research

Immobilization of fumarase from thermophilic eukaryotic red alga Cyanidioschyzon merolae on ceramic carrier
J Gen Appl Microbiol. 2024 Feb 29. doi: 10.2323/jgam.2024.02.003. Online ahead of print.ABSTRACTFumarase is an enzyme catalyzing reversible reaction between fumarate and L-malate in the citric acid cycle. Fumarase is used in the industrial production of L-malate, and its immobilization is required for reuse of the fumarases to reduce the cost. Accordingly, understanding the properties of immobilized fumarase is crucial, and several groups report on the storage stability and kinetic parameters of immobilized fumarase. Here we have immobilized fumarase from the thermophilic red alga Cyanidioschyzon merolae (CmFUM) on ceramic...
Source: Journal of General and Applied Microbiology - February 28, 2024 Category: Microbiology Authors: Miyo Yamane Kaori Iwazumi Takashi Osanai Source Type: research