Decorating phenylalanine side-chains with triple labeled < sup > 13 < /sup > C/ < sup > 19 < /sup > F/ < sup > 2 < /sup > H isotope patterns
We present an economic and straightforward method to introduce 13C-19F spin systems into the deuterated aromatic side chains of phenylalanine as reporters for various protein NMR applications. The method is based on the synthesis of [4-13C, 2,3,5,6-2H4] 4-fluorophenylalanine from the commercially available isotope sources [2-13C] acetone and deuterium oxide. This compound is readily metabolized by standard Escherichia coli overexpression in a glyphosate-containing minimal medium, which results in high incorporation rates in the corresponding target proteins.PMID:38509441 | DOI:10.1007/s10858-024-00440-z (Source: Journal of Bimolecular NMR)
Source: Journal of Bimolecular NMR - March 21, 2024 Category: Biomedical Science Authors: Giorgia Toscano Julian Holzinger Benjamin Nagl Georg Kontaxis Hanspeter K ählig Robert Konrat Roman J Lichtenecker Source Type: research
Decorating phenylalanine side-chains with triple labeled < sup > 13 < /sup > C/ < sup > 19 < /sup > F/ < sup > 2 < /sup > H isotope patterns
We present an economic and straightforward method to introduce 13C-19F spin systems into the deuterated aromatic side chains of phenylalanine as reporters for various protein NMR applications. The method is based on the synthesis of [4-13C, 2,3,5,6-2H4] 4-fluorophenylalanine from the commercially available isotope sources [2-13C] acetone and deuterium oxide. This compound is readily metabolized by standard Escherichia coli overexpression in a glyphosate-containing minimal medium, which results in high incorporation rates in the corresponding target proteins.PMID:38509441 | DOI:10.1007/s10858-024-00440-z (Source: Journal of Bimolecular NMR)
Source: Journal of Bimolecular NMR - March 21, 2024 Category: Biomedical Science Authors: Giorgia Toscano Julian Holzinger Benjamin Nagl Georg Kontaxis Hanspeter K ählig Robert Konrat Roman J Lichtenecker Source Type: research
Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
J Biomol NMR. 2024 Feb 29. doi: 10.1007/s10858-024-00437-8. Online ahead of print.ABSTRACTN-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains of life. In humans, genetic defects in N-linked glycosylation pathways result in metabolic diseases collectively called Congenital Disorders of Glycosylation. In this modification reaction, a mannose rich oligosaccharide is transferred from a lipid-linked donor substrate to a specific asparagine side-chain within the -N-X-T/S- sequence (where X ≠ Proline) of the nascent protein. Oligosaccharyltransferase (OST), ...
Source: Journal of Bimolecular NMR - February 29, 2024 Category: Biomedical Science Authors: Bharat P Chaudhary Jochem Struppe Hem Moktan David Zoetewey Donghua H Zhou Smita Mohanty Source Type: research
Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
J Biomol NMR. 2024 Feb 29. doi: 10.1007/s10858-024-00437-8. Online ahead of print.ABSTRACTN-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains of life. In humans, genetic defects in N-linked glycosylation pathways result in metabolic diseases collectively called Congenital Disorders of Glycosylation. In this modification reaction, a mannose rich oligosaccharide is transferred from a lipid-linked donor substrate to a specific asparagine side-chain within the -N-X-T/S- sequence (where X ≠ Proline) of the nascent protein. Oligosaccharyltransferase (OST), ...
Source: Journal of Bimolecular NMR - February 29, 2024 Category: Biomedical Science Authors: Bharat P Chaudhary Jochem Struppe Hem Moktan David Zoetewey Donghua H Zhou Smita Mohanty Source Type: research
Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
J Biomol NMR. 2024 Feb 29. doi: 10.1007/s10858-024-00437-8. Online ahead of print.ABSTRACTN-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains of life. In humans, genetic defects in N-linked glycosylation pathways result in metabolic diseases collectively called Congenital Disorders of Glycosylation. In this modification reaction, a mannose rich oligosaccharide is transferred from a lipid-linked donor substrate to a specific asparagine side-chain within the -N-X-T/S- sequence (where X ≠ Proline) of the nascent protein. Oligosaccharyltransferase (OST), ...
Source: Journal of Bimolecular NMR - February 29, 2024 Category: Biomedical Science Authors: Bharat P Chaudhary Jochem Struppe Hem Moktan David Zoetewey Donghua H Zhou Smita Mohanty Source Type: research
Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
J Biomol NMR. 2024 Feb 29. doi: 10.1007/s10858-024-00437-8. Online ahead of print.ABSTRACTN-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains of life. In humans, genetic defects in N-linked glycosylation pathways result in metabolic diseases collectively called Congenital Disorders of Glycosylation. In this modification reaction, a mannose rich oligosaccharide is transferred from a lipid-linked donor substrate to a specific asparagine side-chain within the -N-X-T/S- sequence (where X ≠ Proline) of the nascent protein. Oligosaccharyltransferase (OST), ...
Source: Journal of Bimolecular NMR - February 29, 2024 Category: Biomedical Science Authors: Bharat P Chaudhary Jochem Struppe Hem Moktan David Zoetewey Donghua H Zhou Smita Mohanty Source Type: research
Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
J Biomol NMR. 2024 Feb 29. doi: 10.1007/s10858-024-00437-8. Online ahead of print.ABSTRACTN-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains of life. In humans, genetic defects in N-linked glycosylation pathways result in metabolic diseases collectively called Congenital Disorders of Glycosylation. In this modification reaction, a mannose rich oligosaccharide is transferred from a lipid-linked donor substrate to a specific asparagine side-chain within the -N-X-T/S- sequence (where X ≠ Proline) of the nascent protein. Oligosaccharyltransferase (OST), ...
Source: Journal of Bimolecular NMR - February 29, 2024 Category: Biomedical Science Authors: Bharat P Chaudhary Jochem Struppe Hem Moktan David Zoetewey Donghua H Zhou Smita Mohanty Source Type: research
Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
J Biomol NMR. 2024 Feb 29. doi: 10.1007/s10858-024-00437-8. Online ahead of print.ABSTRACTN-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains of life. In humans, genetic defects in N-linked glycosylation pathways result in metabolic diseases collectively called Congenital Disorders of Glycosylation. In this modification reaction, a mannose rich oligosaccharide is transferred from a lipid-linked donor substrate to a specific asparagine side-chain within the -N-X-T/S- sequence (where X ≠ Proline) of the nascent protein. Oligosaccharyltransferase (OST), ...
Source: Journal of Bimolecular NMR - February 29, 2024 Category: Biomedical Science Authors: Bharat P Chaudhary Jochem Struppe Hem Moktan David Zoetewey Donghua H Zhou Smita Mohanty Source Type: research
Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
J Biomol NMR. 2024 Feb 29. doi: 10.1007/s10858-024-00437-8. Online ahead of print.ABSTRACTN-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains of life. In humans, genetic defects in N-linked glycosylation pathways result in metabolic diseases collectively called Congenital Disorders of Glycosylation. In this modification reaction, a mannose rich oligosaccharide is transferred from a lipid-linked donor substrate to a specific asparagine side-chain within the -N-X-T/S- sequence (where X ≠ Proline) of the nascent protein. Oligosaccharyltransferase (OST), ...
Source: Journal of Bimolecular NMR - February 29, 2024 Category: Biomedical Science Authors: Bharat P Chaudhary Jochem Struppe Hem Moktan David Zoetewey Donghua H Zhou Smita Mohanty Source Type: research
Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
J Biomol NMR. 2024 Feb 29. doi: 10.1007/s10858-024-00437-8. Online ahead of print.ABSTRACTN-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains of life. In humans, genetic defects in N-linked glycosylation pathways result in metabolic diseases collectively called Congenital Disorders of Glycosylation. In this modification reaction, a mannose rich oligosaccharide is transferred from a lipid-linked donor substrate to a specific asparagine side-chain within the -N-X-T/S- sequence (where X ≠ Proline) of the nascent protein. Oligosaccharyltransferase (OST), ...
Source: Journal of Bimolecular NMR - February 29, 2024 Category: Biomedical Science Authors: Bharat P Chaudhary Jochem Struppe Hem Moktan David Zoetewey Donghua H Zhou Smita Mohanty Source Type: research
Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
J Biomol NMR. 2024 Feb 29. doi: 10.1007/s10858-024-00437-8. Online ahead of print.ABSTRACTN-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains of life. In humans, genetic defects in N-linked glycosylation pathways result in metabolic diseases collectively called Congenital Disorders of Glycosylation. In this modification reaction, a mannose rich oligosaccharide is transferred from a lipid-linked donor substrate to a specific asparagine side-chain within the -N-X-T/S- sequence (where X ≠ Proline) of the nascent protein. Oligosaccharyltransferase (OST), ...
Source: Journal of Bimolecular NMR - February 29, 2024 Category: Biomedical Science Authors: Bharat P Chaudhary Jochem Struppe Hem Moktan David Zoetewey Donghua H Zhou Smita Mohanty Source Type: research
Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
J Biomol NMR. 2024 Feb 29. doi: 10.1007/s10858-024-00437-8. Online ahead of print.ABSTRACTN-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains of life. In humans, genetic defects in N-linked glycosylation pathways result in metabolic diseases collectively called Congenital Disorders of Glycosylation. In this modification reaction, a mannose rich oligosaccharide is transferred from a lipid-linked donor substrate to a specific asparagine side-chain within the -N-X-T/S- sequence (where X ≠ Proline) of the nascent protein. Oligosaccharyltransferase (OST), ...
Source: Journal of Bimolecular NMR - February 29, 2024 Category: Biomedical Science Authors: Bharat P Chaudhary Jochem Struppe Hem Moktan David Zoetewey Donghua H Zhou Smita Mohanty Source Type: research
Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
J Biomol NMR. 2024 Feb 29. doi: 10.1007/s10858-024-00437-8. Online ahead of print.ABSTRACTN-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains of life. In humans, genetic defects in N-linked glycosylation pathways result in metabolic diseases collectively called Congenital Disorders of Glycosylation. In this modification reaction, a mannose rich oligosaccharide is transferred from a lipid-linked donor substrate to a specific asparagine side-chain within the -N-X-T/S- sequence (where X ≠ Proline) of the nascent protein. Oligosaccharyltransferase (OST), ...
Source: Journal of Bimolecular NMR - February 29, 2024 Category: Biomedical Science Authors: Bharat P Chaudhary Jochem Struppe Hem Moktan David Zoetewey Donghua H Zhou Smita Mohanty Source Type: research
Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
J Biomol NMR. 2024 Feb 29. doi: 10.1007/s10858-024-00437-8. Online ahead of print.ABSTRACTN-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains of life. In humans, genetic defects in N-linked glycosylation pathways result in metabolic diseases collectively called Congenital Disorders of Glycosylation. In this modification reaction, a mannose rich oligosaccharide is transferred from a lipid-linked donor substrate to a specific asparagine side-chain within the -N-X-T/S- sequence (where X ≠ Proline) of the nascent protein. Oligosaccharyltransferase (OST), ...
Source: Journal of Bimolecular NMR - February 29, 2024 Category: Biomedical Science Authors: Bharat P Chaudhary Jochem Struppe Hem Moktan David Zoetewey Donghua H Zhou Smita Mohanty Source Type: research
Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
J Biomol NMR. 2024 Feb 29. doi: 10.1007/s10858-024-00437-8. Online ahead of print.ABSTRACTN-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains of life. In humans, genetic defects in N-linked glycosylation pathways result in metabolic diseases collectively called Congenital Disorders of Glycosylation. In this modification reaction, a mannose rich oligosaccharide is transferred from a lipid-linked donor substrate to a specific asparagine side-chain within the -N-X-T/S- sequence (where X ≠ Proline) of the nascent protein. Oligosaccharyltransferase (OST), ...
Source: Journal of Bimolecular NMR - February 29, 2024 Category: Biomedical Science Authors: Bharat P Chaudhary Jochem Struppe Hem Moktan David Zoetewey Donghua H Zhou Smita Mohanty Source Type: research
