Decorating phenylalanine side-chains with triple labeled < sup > 13 < /sup > C/ < sup > 19 < /sup > F/ < sup > 2 < /sup > H isotope patterns

We present an economic and straightforward method to introduce 13C-19F spin systems into the deuterated aromatic side chains of phenylalanine as reporters for various protein NMR applications. The method is based on the synthesis of [4-13C, 2,3,5,6-2H4] 4-fluorophenylalanine from the commercially available isotope sources [2-13C] acetone and deuterium oxide. This compound is readily metabolized by standard Escherichia coli overexpression in a glyphosate-containing minimal medium, which results in high incorporation rates in the corresponding target proteins.PMID:38509441 | DOI:10.1007/s10858-024-00440-z
Source: Journal of Bimolecular NMR - Category: Biomedical Science Authors: Source Type: research