Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
J Biomol NMR. 2024 Mar 30. doi: 10.1007/s10858-024-00439-6. Online ahead of print.ABSTRACTIn NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensitivity. Therefore, 19F-labeling procedures are of wide-spread interest. Here, we use 5-fluoroindole as a precursor for cost-effective residue-specific introduction of 5-fluorotryptophan (5F-Trp) into G protein-coupled receptors (GPCRs) expressed in Pichia pastoris. The method was successfully implemented with the neurokinin 1 receptor (NK1R). The 19F-NMR spectra of 5F-Trp-labeled NK1R showed one well-separated high...
Source: Journal of Bimolecular NMR - March 30, 2024 Category: Biomedical Science Authors: Benxun Pan Canyong Guo Dongsheng Liu Kurt W üthrich Source Type: research
Enabling site-specific NMR investigations of therapeutic Fab using a cell-free based isotopic labeling approach: application to anti-LAMP1 Fab
J Biomol NMR. 2024 Mar 28. doi: 10.1007/s10858-023-00433-4. Online ahead of print.ABSTRACTMonoclonal antibodies (mAbs) are biotherapeutics that have achieved outstanding success in treating many life-threatening and chronic diseases. The recognition of an antigen is mediated by the fragment antigen binding (Fab) regions composed by four different disulfide bridge-linked immunoglobulin domains. NMR is a powerful method to assess the integrity, the structure and interaction of Fabs, but site specific analysis has been so far hampered by the size of the Fabs and the lack of approaches to produce isotopically labeled samples. ...
Source: Journal of Bimolecular NMR - March 28, 2024 Category: Biomedical Science Authors: Arthur Giraud Lionel Imbert Adrien Favier Faustine Henot Francis Duffieux Camille Samson Oriane Frances Elodie Crublet J érôme Boisbouvier Source Type: research
