Strategies for acquisition of resonance assignment spectra of highly dynamic membrane proteins: a GPCR case study
J Biomol NMR. 2023 Aug;77(4):191-202. doi: 10.1007/s10858-023-00421-8. Epub 2023 Jul 26.ABSTRACTIn protein nuclear magnetic resonance (NMR), chemical shift assignment provides a wealth of information. However, acquisition of high-quality solid-state NMR spectra depends on protein-specific dynamics. For membrane proteins, bilayer heterogeneity further complicates this observation. Since the efficiency of cross-polarization transfer is strongly entwined with protein dynamics, optimal temperatures for spectral sensitivity and resolution will depend not only on inherent protein dynamics, but temperature-dependent phase propert...
Source: Journal of Bimolecular NMR - July 26, 2023 Category: Biomedical Science Authors: Evan J van Aalst Jun Jang Ty C Halligan Benjamin J Wylie Source Type: research
Strategies for acquisition of resonance assignment spectra of highly dynamic membrane proteins: a GPCR case study
J Biomol NMR. 2023 Aug;77(4):191-202. doi: 10.1007/s10858-023-00421-8. Epub 2023 Jul 26.ABSTRACTIn protein nuclear magnetic resonance (NMR), chemical shift assignment provides a wealth of information. However, acquisition of high-quality solid-state NMR spectra depends on protein-specific dynamics. For membrane proteins, bilayer heterogeneity further complicates this observation. Since the efficiency of cross-polarization transfer is strongly entwined with protein dynamics, optimal temperatures for spectral sensitivity and resolution will depend not only on inherent protein dynamics, but temperature-dependent phase propert...
Source: Journal of Bimolecular NMR - July 26, 2023 Category: Biomedical Science Authors: Evan J van Aalst Jun Jang Ty C Halligan Benjamin J Wylie Source Type: research
Strategies for acquisition of resonance assignment spectra of highly dynamic membrane proteins: a GPCR case study
J Biomol NMR. 2023 Jul 26. doi: 10.1007/s10858-023-00421-8. Online ahead of print.ABSTRACTIn protein nuclear magnetic resonance (NMR), chemical shift assignment provides a wealth of information. However, acquisition of high-quality solid-state NMR spectra depends on protein-specific dynamics. For membrane proteins, bilayer heterogeneity further complicates this observation. Since the efficiency of cross-polarization transfer is strongly entwined with protein dynamics, optimal temperatures for spectral sensitivity and resolution will depend not only on inherent protein dynamics, but temperature-dependent phase properties of...
Source: Journal of Bimolecular NMR - July 26, 2023 Category: Biomedical Science Authors: Evan J van Aalst Jun Jang Ty C Halligan Benjamin J Wylie Source Type: research
Strategies for acquisition of resonance assignment spectra of highly dynamic membrane proteins: a GPCR case study
J Biomol NMR. 2023 Jul 26. doi: 10.1007/s10858-023-00421-8. Online ahead of print.ABSTRACTIn protein nuclear magnetic resonance (NMR), chemical shift assignment provides a wealth of information. However, acquisition of high-quality solid-state NMR spectra depends on protein-specific dynamics. For membrane proteins, bilayer heterogeneity further complicates this observation. Since the efficiency of cross-polarization transfer is strongly entwined with protein dynamics, optimal temperatures for spectral sensitivity and resolution will depend not only on inherent protein dynamics, but temperature-dependent phase properties of...
Source: Journal of Bimolecular NMR - July 26, 2023 Category: Biomedical Science Authors: Evan J van Aalst Jun Jang Ty C Halligan Benjamin J Wylie Source Type: research
Strategies for acquisition of resonance assignment spectra of highly dynamic membrane proteins: a GPCR case study
J Biomol NMR. 2023 Jul 26. doi: 10.1007/s10858-023-00421-8. Online ahead of print.ABSTRACTIn protein nuclear magnetic resonance (NMR), chemical shift assignment provides a wealth of information. However, acquisition of high-quality solid-state NMR spectra depends on protein-specific dynamics. For membrane proteins, bilayer heterogeneity further complicates this observation. Since the efficiency of cross-polarization transfer is strongly entwined with protein dynamics, optimal temperatures for spectral sensitivity and resolution will depend not only on inherent protein dynamics, but temperature-dependent phase properties of...
Source: Journal of Bimolecular NMR - July 26, 2023 Category: Biomedical Science Authors: Evan J van Aalst Jun Jang Ty C Halligan Benjamin J Wylie Source Type: research
Strategies for acquisition of resonance assignment spectra of highly dynamic membrane proteins: a GPCR case study
J Biomol NMR. 2023 Jul 26. doi: 10.1007/s10858-023-00421-8. Online ahead of print.ABSTRACTIn protein nuclear magnetic resonance (NMR), chemical shift assignment provides a wealth of information. However, acquisition of high-quality solid-state NMR spectra depends on protein-specific dynamics. For membrane proteins, bilayer heterogeneity further complicates this observation. Since the efficiency of cross-polarization transfer is strongly entwined with protein dynamics, optimal temperatures for spectral sensitivity and resolution will depend not only on inherent protein dynamics, but temperature-dependent phase properties of...
Source: Journal of Bimolecular NMR - July 26, 2023 Category: Biomedical Science Authors: Evan J van Aalst Jun Jang Ty C Halligan Benjamin J Wylie Source Type: research
Strategies for acquisition of resonance assignment spectra of highly dynamic membrane proteins: a GPCR case study
J Biomol NMR. 2023 Jul 26. doi: 10.1007/s10858-023-00421-8. Online ahead of print.ABSTRACTIn protein nuclear magnetic resonance (NMR), chemical shift assignment provides a wealth of information. However, acquisition of high-quality solid-state NMR spectra depends on protein-specific dynamics. For membrane proteins, bilayer heterogeneity further complicates this observation. Since the efficiency of cross-polarization transfer is strongly entwined with protein dynamics, optimal temperatures for spectral sensitivity and resolution will depend not only on inherent protein dynamics, but temperature-dependent phase properties of...
Source: Journal of Bimolecular NMR - July 26, 2023 Category: Biomedical Science Authors: Evan J van Aalst Jun Jang Ty C Halligan Benjamin J Wylie Source Type: research
Strategies for acquisition of resonance assignment spectra of highly dynamic membrane proteins: a GPCR case study
J Biomol NMR. 2023 Jul 26. doi: 10.1007/s10858-023-00421-8. Online ahead of print.ABSTRACTIn protein nuclear magnetic resonance (NMR), chemical shift assignment provides a wealth of information. However, acquisition of high-quality solid-state NMR spectra depends on protein-specific dynamics. For membrane proteins, bilayer heterogeneity further complicates this observation. Since the efficiency of cross-polarization transfer is strongly entwined with protein dynamics, optimal temperatures for spectral sensitivity and resolution will depend not only on inherent protein dynamics, but temperature-dependent phase properties of...
Source: Journal of Bimolecular NMR - July 26, 2023 Category: Biomedical Science Authors: Evan J van Aalst Jun Jang Ty C Halligan Benjamin J Wylie Source Type: research
Strategies for acquisition of resonance assignment spectra of highly dynamic membrane proteins: a GPCR case study
J Biomol NMR. 2023 Jul 26. doi: 10.1007/s10858-023-00421-8. Online ahead of print.ABSTRACTIn protein nuclear magnetic resonance (NMR), chemical shift assignment provides a wealth of information. However, acquisition of high-quality solid-state NMR spectra depends on protein-specific dynamics. For membrane proteins, bilayer heterogeneity further complicates this observation. Since the efficiency of cross-polarization transfer is strongly entwined with protein dynamics, optimal temperatures for spectral sensitivity and resolution will depend not only on inherent protein dynamics, but temperature-dependent phase properties of...
Source: Journal of Bimolecular NMR - July 26, 2023 Category: Biomedical Science Authors: Evan J van Aalst Jun Jang Ty C Halligan Benjamin J Wylie Source Type: research
Strategies for acquisition of resonance assignment spectra of highly dynamic membrane proteins: a GPCR case study
J Biomol NMR. 2023 Jul 26. doi: 10.1007/s10858-023-00421-8. Online ahead of print.ABSTRACTIn protein nuclear magnetic resonance (NMR), chemical shift assignment provides a wealth of information. However, acquisition of high-quality solid-state NMR spectra depends on protein-specific dynamics. For membrane proteins, bilayer heterogeneity further complicates this observation. Since the efficiency of cross-polarization transfer is strongly entwined with protein dynamics, optimal temperatures for spectral sensitivity and resolution will depend not only on inherent protein dynamics, but temperature-dependent phase properties of...
Source: Journal of Bimolecular NMR - July 26, 2023 Category: Biomedical Science Authors: Evan J van Aalst Jun Jang Ty C Halligan Benjamin J Wylie Source Type: research
Improved spectral resolution of [ < sup > 13 < /sup > C, < sup > 1 < /sup > H]-HSQC spectra of aromatic amino acid residues in proteins produced by cell-free synthesis from inexpensive < sup > 13 < /sup > C-labelled precursors
J Biomol NMR. 2023 Jun 20. doi: 10.1007/s10858-023-00420-9. Online ahead of print.ABSTRACTCell-free protein synthesis using eCells allows production of amino acids from inexpensive 13C-labelled precursors. We show that the metabolic pathway converting pyruvate, glucose and erythrose into aromatic amino acids is maintained in eCells. Judicious choice of 13C-labelled starting material leads to proteins, where the sidechains of aromatic amino acids display [13C,1H]-HSQC cross-peaks free of one-bond 13C-13C couplings. Selective 13C-labelling of tyrosine and phenylalanine residues is achieved simply by using different compositi...
Source: Journal of Bimolecular NMR - June 20, 2023 Category: Biomedical Science Authors: Damian Van Raad Thomas Huber Gottfried Otting Source Type: research
Improved spectral resolution of [ < sup > 13 < /sup > C, < sup > 1 < /sup > H]-HSQC spectra of aromatic amino acid residues in proteins produced by cell-free synthesis from inexpensive < sup > 13 < /sup > C-labelled precursors
J Biomol NMR. 2023 Jun 20. doi: 10.1007/s10858-023-00420-9. Online ahead of print.ABSTRACTCell-free protein synthesis using eCells allows production of amino acids from inexpensive 13C-labelled precursors. We show that the metabolic pathway converting pyruvate, glucose and erythrose into aromatic amino acids is maintained in eCells. Judicious choice of 13C-labelled starting material leads to proteins, where the sidechains of aromatic amino acids display [13C,1H]-HSQC cross-peaks free of one-bond 13C-13C couplings. Selective 13C-labelling of tyrosine and phenylalanine residues is achieved simply by using different compositi...
Source: Journal of Bimolecular NMR - June 20, 2023 Category: Biomedical Science Authors: Damian Van Raad Thomas Huber Gottfried Otting Source Type: research
Improved spectral resolution of [ < sup > 13 < /sup > C, < sup > 1 < /sup > H]-HSQC spectra of aromatic amino acid residues in proteins produced by cell-free synthesis from inexpensive < sup > 13 < /sup > C-labelled precursors
J Biomol NMR. 2023 Jun 20. doi: 10.1007/s10858-023-00420-9. Online ahead of print.ABSTRACTCell-free protein synthesis using eCells allows production of amino acids from inexpensive 13C-labelled precursors. We show that the metabolic pathway converting pyruvate, glucose and erythrose into aromatic amino acids is maintained in eCells. Judicious choice of 13C-labelled starting material leads to proteins, where the sidechains of aromatic amino acids display [13C,1H]-HSQC cross-peaks free of one-bond 13C-13C couplings. Selective 13C-labelling of tyrosine and phenylalanine residues is achieved simply by using different compositi...
Source: Journal of Bimolecular NMR - June 20, 2023 Category: Biomedical Science Authors: Damian Van Raad Thomas Huber Gottfried Otting Source Type: research
Improved spectral resolution of [ < sup > 13 < /sup > C, < sup > 1 < /sup > H]-HSQC spectra of aromatic amino acid residues in proteins produced by cell-free synthesis from inexpensive < sup > 13 < /sup > C-labelled precursors
J Biomol NMR. 2023 Jun 20. doi: 10.1007/s10858-023-00420-9. Online ahead of print.ABSTRACTCell-free protein synthesis using eCells allows production of amino acids from inexpensive 13C-labelled precursors. We show that the metabolic pathway converting pyruvate, glucose and erythrose into aromatic amino acids is maintained in eCells. Judicious choice of 13C-labelled starting material leads to proteins, where the sidechains of aromatic amino acids display [13C,1H]-HSQC cross-peaks free of one-bond 13C-13C couplings. Selective 13C-labelling of tyrosine and phenylalanine residues is achieved simply by using different compositi...
Source: Journal of Bimolecular NMR - June 20, 2023 Category: Biomedical Science Authors: Damian Van Raad Thomas Huber Gottfried Otting Source Type: research
Improved spectral resolution of [ < sup > 13 < /sup > C, < sup > 1 < /sup > H]-HSQC spectra of aromatic amino acid residues in proteins produced by cell-free synthesis from inexpensive < sup > 13 < /sup > C-labelled precursors
J Biomol NMR. 2023 Jun 20. doi: 10.1007/s10858-023-00420-9. Online ahead of print.ABSTRACTCell-free protein synthesis using eCells allows production of amino acids from inexpensive 13C-labelled precursors. We show that the metabolic pathway converting pyruvate, glucose and erythrose into aromatic amino acids is maintained in eCells. Judicious choice of 13C-labelled starting material leads to proteins, where the sidechains of aromatic amino acids display [13C,1H]-HSQC cross-peaks free of one-bond 13C-13C couplings. Selective 13C-labelling of tyrosine and phenylalanine residues is achieved simply by using different compositi...
Source: Journal of Bimolecular NMR - June 20, 2023 Category: Biomedical Science Authors: Damian Van Raad Thomas Huber Gottfried Otting Source Type: research
