Frailty predicts postoperative complications following pancreaticoduodenectomy
Introduction: Frailty is a clinically recognizable state of decreased reserve and function across physiologic systems, characterized by an inability to cope with acute stressors. A validated modified frailty index (mFI) was used to evaluate the impact of frailty on postoperative complications following pancreaticoduodenectomy.
Methods: Data from consecutive patients undergoing pancreaticoduodenectomy from 2011-2020 was collected retrospectively at a high-volume tertiary care hepatopancreatobiliary hospital. Based on an 11-item mFI, patients were grouped by high ( ≥0.27) and low mFI. The main outcome was postoperative co...
Source: European Surgical Research - February 16, 2022 Category: Surgery Source Type: research
Left Ventricular Wall Reconstruction with Autologous Vascularized Tunica Muscularis of Stomach in a Porcine Pilot Model
Conclusions: The clinical results and the observed physiological integration of gastric grafts into the cardiac structure demonstrate the feasibility of vascularized stomach tissue as myocardial prosthesis. The physiological remodeling indicates a regenerative potential of the graft. Above all, the connection of the gastric vessels with the coronary system constitutes a rationale for the use of vascularized and, therefore, viable stomach tissue for versatile tissue engineering applications. (Source: European Surgical Research)
Source: European Surgical Research - February 8, 2022 Category: Surgery Source Type: research
Left Ventricular Wall Reconstruction with Autologous Vascularized Gastric Graft in a Porcine Pilot Model
Conclusions: The clinical results and the observed physiological integration of gastric grafts into the cardiac structure demonstrate the feasibility of vascularized stomach tissue as a myocardial prosthesis. The physiological remodeling indicates a regenerative potential of the graft. Above all, the connection of the gastric vessels with the coronary system constitutes a rationale for the use of vascularized and therefore viable stomach tissue for versatile tissue engineering applications. (Source: European Surgical Research)
Source: European Surgical Research - February 8, 2022 Category: Surgery Source Type: research
Long-term outcomes of left versus right laparoscopic living donor nephrectomy with multiple renal arteries
Conclusions: Both right donor nephrectomy and left donor nephrectomy are safe procedures with no significant negatively impacted rates for neither survival nor complications of the recipients in the long-term, compared to single renal artery ones. (Source: European Surgical Research)
Source: European Surgical Research - January 31, 2022 Category: Surgery Source Type: research
Toward evidence-based severity assessment in mouse models with repeated seizures: (II.) Impact of surgery and intrahippocampal kainate
Conclusion:
In conclusion, our findings argue against the pronounced impact of highly frequent electrographic seizures on the well-being of mice. However, an increased level of nervousness in a subgroup fo animals should be considered for handling procedures and refinement measures. In the early post-surgical phase several parameters indicate an influence of the interventions with evidence that the non-convulsive status epilepticus can negatively affect the recovery. Thus, the development and validation of refinement efforts should focus on this experimental phase.
Finally, the data sets suggest that simple readout param...
Source: European Surgical Research - January 24, 2022 Category: Surgery Source Type: research
Potentials of CCL21 and CBS as therapeutic approaches for breast cancer
Objective: The present research set out to ascertain the roles of CCL21 and CBS in breast cancer (BC) cell biological behaviors and the relationship of CCL21 and CBS expression with the clinicopathological features of patients with BC.
Methods: Immunohistochemistry of CCL21 or CBS was performed in 18 intraductal cancer tissues, 124 invasive BC tissues, 50 paraneoplastic tissues, 50 lobular hyperplasia tissues, and 30 normal breast tissues. For cell experiments, two human BC cell lines (MDA-MB-231 and MCF-7) and a human breast epithelial cell line (MCF-10A) were utilized to detect the expression of CCL21 and CBS. After los...
Source: European Surgical Research - January 13, 2022 Category: Surgery Source Type: research
miR-27b-3p Improved High Glucose-Induced Spermatogenic Cell Damage via Regulating Gfpt1/HBP Signaling
Conclusion: miR-27b-3p could improve HG-induced spermatogenic cell damage via regulating Gfpt1/HBP signaling, providing a new treatment strategy for the treatment of DM-induced testicular damage.Eur Surg Res (Source: European Surgical Research)
Source: European Surgical Research - January 5, 2022 Category: Surgery Source Type: research
Alterations in Toll-Like Receptor 7 and -9 mRNA Levels in Lungs after Ovariohysterectomy in a Pyometra Mouse Model
Conclusions: This is the first report of immunohistochemical TLR-7 and -9 localization and of TLR-7, -9, MYD88, and NF κB mRNA expression in the uterus causing lung injury in a P mouse model.Eur Surg Res (Source: European Surgical Research)
Source: European Surgical Research - December 27, 2021 Category: Surgery Source Type: research
Mitochondrial Dysfunction in Trauma-Related Coagulopathy: Is There Causality? Study Protocol for a Prospective Observational Study
Hemorrhage control often poses a great challenge for clinicians due to trauma-induced coagulopathy (TIC). The pathogenesis of TIC is not completely revealed; however, growing evidence attributes a central role to altered platelet biology. The activation of thrombocytes and subsequent clot formation are highly energetic processes being tied to mitochondrial activity, and the inhibition of the electron transport chain (ETC) impedes on thrombogenesis, suggesting the potential role of mitochondria in TIC. Our present study protocol provides a guide to quantitatively characterize the derangements of mitochondrial functions in T...
Source: European Surgical Research - December 24, 2021 Category: Surgery Source Type: research
Mitochondrial dysfunction in trauma-related coagulopathy – Is there causality? – Study protocol for a prospective observational study
Hemorrhage control often poses a great challenge for clinicians due to trauma-induced coagulopathy (TIC). The pathogenesis of TIC is not completely revealed; however, growing evidence attributes a central role to altered platelet biology. The activation of thrombocytes and subsequent clot formation are highly energetic processes being tied to mitochondrial activity, and the inhibition of the electron transport chain (ETC) impedes on thrombogenesis, suggesting the potential role of mitochondria in TIC. Our present study protocol provides a guide to quantitatively characterize the derangements of mitochondrial functions in T...
Source: European Surgical Research - December 24, 2021 Category: Surgery Source Type: research
Involvement of Cannabinoid Type 2 Receptors in the Favorable Effects of Sumatriptan on the Random-Pattern Skin Flap Survival in Rats: A Novel Potential Target
Conclusion: We discovered that CB2 receptors play a crucial role in the favorable effects of sumatriptan on skin flap survival as a novel mechanism of action. So, targeting these receptors seems to be a dependable method in skin flap surgeries to ensure its survival and prevent tissue necrosis. Further experimental and clinical investigations are needed to ensure the safe clinical application of this method.Eur Surg Res (Source: European Surgical Research)
Source: European Surgical Research - December 20, 2021 Category: Surgery Source Type: research
Involvement of cannabinoid type 2 (CB2) receptors in the favorable effects of sumatriptan on the random-pattern skin flap survival in rats: a novel potential target
In this study, we aimed to evaluate the possible involvement of CB2 receptors in the anti-inflammatory effects of sumatriptan and improvement of the random-pattern skin flap survival in rats.
Methods: In a controlled experimental study, 36 male Wistar rats were randomly divided into six study groups (n = 6 per group). Two doses of sumatriptan (0.1 and 0.3 mg/kg) were administered intraperitoneally (i.p) 30 minutes before harvesting the flap tissue. In a separate group, SR144528 (a selective CB2 receptor inverse agonist) was injected before the most effective dose of sumatriptan to determine the possible involvement of CB2...
Source: European Surgical Research - December 20, 2021 Category: Surgery Source Type: research
The Dot-Probe Attention Bias Task as a Method to Assess Psychological Well-Being after Anesthesia: A Study with Adult Female Long-Tailed Macaques ( < b > < i > Macaca fascicularis < /i > < /b > )
Understanding the impact routine research and laboratory procedures have on animals is crucial to improving their well-being and to the success and reproducibility of the research they are involved in. Cognitive measures of welfare offer insight into animals ’ internal psychological state, but require validation. Attention bias – the tendency to attend to one type of information over another – is a cognitive phenomenon documented in humans and animals that is known to be modulated by affective state (i.e., emotions). Hence, changes in attention bi as may offer researchers a deeper perspective of their animals’ psyc...
Source: European Surgical Research - December 16, 2021 Category: Surgery Source Type: research
The dot-probe attention bias task as a method to assess psychological wellbeing after anesthesia: A study with adult female long-tailed macaques (Macaca fascicularis)
Understanding the impact routine research and laboratory procedures have on animals is crucial to improving their wellbeing and to the success and reproducibility of the research they are involved in. Cognitive measures of welfare offer insight into animals ’ internal psychological state, but require validation. Attention bias - the tendency to attend to one type of information over another – is a cognitive phenomenon documented in humans and animals that is known to be modulated by affective state (i.e., emotions). Hence, changes in attention bias may offer researchers a deeper perspective of their animals’ psycholo...
Source: European Surgical Research - December 16, 2021 Category: Surgery Source Type: research
Smartphone-based DIY home microsurgical training with 3D printed microvascular clamps and Japanese noodles
We have recently incorporated simple modifications of the konjac flour noodle model to enable DIY home microsurgical training by (i) placing a smartphone on a mug to act as a microscope with at least 3.5-5x magnification, and (ii) rather than cannulating with a 22G needle as described by others, we have found that cannulation with a 23G needle followed by a second pass with an 18G needle will create a lumen (approx. 0.83 mm) without an overly thick and unrealistic “vessel” wall. The current set-up however, did not allow realistic evaluation of anastomotic patency as the noodles became macerated after application of sta...
Source: European Surgical Research - December 15, 2021 Category: Surgery Source Type: research
