An essential role for the Ino80 chromatin remodeling complex in regulation of gene expression during cellular quiescence
Chromosome Res. 2023 Apr 12;31(2):14. doi: 10.1007/s10577-023-09723-x.ABSTRACTCellular quiescence is an important physiological state both in unicellular and multicellular eukaryotes. Quiescent cells are halted for proliferation and stop the cell cycle at the G0 stage. Using fission yeast as a model organism, we have previously found that several subunits of a conserved chromatin remodeling complex, Ino80C (INOsitol requiring nucleosome remodeling factor), are required for survival in quiescence. Here, we demonstrate that Ino80C has a key function in the regulation of gene expression in G0 cells. We show that null mutants ...
Source: Chromosome Research - April 12, 2023 Category: Genetics & Stem Cells Authors: Yasaman Zahedi Shengyuan Zeng Karl Ekwall Source Type: research
Chromosome-length genome assemblies and cytogenomic analyses of pangolins reveal remarkable chromosome counts and plasticity
We report the first chromosome-length genome assemblies for three species in the mammalian order Pholidota: the white-bellied, Chinese, and Sunda pangolins. Surprisingly, we observe extraordinary karyotypic plasticity within this order and, in female white-bellied pangolins, the largest number of chromosomes reported in a Laurasiatherian mammal: 2n = 114. We perform the first karyotype analysis of an African pangolin and report a Y-autosome fusion in white-bellied pangolins, resulting in 2n = 113 for males. We employ a novel strategy to confirm the fusion and identify the autosome involved by finding the pseudoautosomal re...
Source: Chromosome Research - April 12, 2023 Category: Genetics & Stem Cells Authors: Marlys L Houck Klaus-Peter Koepfli Taylor Hains Ruqayya Khan Suellen J Charter Julie A Fronczek Ann C Misuraca Sergei Kliver Polina L Perelman Violetta Beklemisheva Alexander Graphodatsky Shu-Jin Luo Stephen J O'Brien Norman T-L Lim Jason S C Chin Vanessa Source Type: research
Characterization of centromeric DNA of Gossypium anomalum reveals sequence-independent enrichment dynamics of centromeric repeats
Chromosome Res. 2023 Mar 27;31(2):12. doi: 10.1007/s10577-023-09721-z.ABSTRACTCentromeres in eukaryotes are composed of highly repetitive DNAs, which evolve rapidly and are thought to achieve a favorable structure in mature centromeres. However, how the centromeric repeat evolves into an adaptive structure is largely unknown. We characterized the centromeric sequences of Gossypium anomalum through chromatin immunoprecipitation against CENH3 antibodies. We revealed that the G. anomalum centromeres contained only retrotransposon-like repeats but were depleted in long arrays of satellites. These retrotransposon-like centromer...
Source: Chromosome Research - March 27, 2023 Category: Genetics & Stem Cells Authors: Wenjie Ding Yuanbin Zhu Jinlei Han Hui Zhang Zhenzhen Xu Haris Khurshid Fang Liu Robert Hasterok Xinlian Shen Kai Wang Source Type: research
Characterization of centromeric DNA of Gossypium anomalum reveals sequence-independent enrichment dynamics of centromeric repeats
Chromosome Res. 2023 Mar 27;31(2):12. doi: 10.1007/s10577-023-09721-z.ABSTRACTCentromeres in eukaryotes are composed of highly repetitive DNAs, which evolve rapidly and are thought to achieve a favorable structure in mature centromeres. However, how the centromeric repeat evolves into an adaptive structure is largely unknown. We characterized the centromeric sequences of Gossypium anomalum through chromatin immunoprecipitation against CENH3 antibodies. We revealed that the G. anomalum centromeres contained only retrotransposon-like repeats but were depleted in long arrays of satellites. These retrotransposon-like centromer...
Source: Chromosome Research - March 27, 2023 Category: Genetics & Stem Cells Authors: Wenjie Ding Yuanbin Zhu Jinlei Han Hui Zhang Zhenzhen Xu Haris Khurshid Fang Liu Robert Hasterok Xinlian Shen Kai Wang Source Type: research
Characterization of centromeric DNA of Gossypium anomalum reveals sequence-independent enrichment dynamics of centromeric repeats
Chromosome Res. 2023 Mar 27;31(2):12. doi: 10.1007/s10577-023-09721-z.ABSTRACTCentromeres in eukaryotes are composed of highly repetitive DNAs, which evolve rapidly and are thought to achieve a favorable structure in mature centromeres. However, how the centromeric repeat evolves into an adaptive structure is largely unknown. We characterized the centromeric sequences of Gossypium anomalum through chromatin immunoprecipitation against CENH3 antibodies. We revealed that the G. anomalum centromeres contained only retrotransposon-like repeats but were depleted in long arrays of satellites. These retrotransposon-like centromer...
Source: Chromosome Research - March 27, 2023 Category: Genetics & Stem Cells Authors: Wenjie Ding Yuanbin Zhu Jinlei Han Hui Zhang Zhenzhen Xu Haris Khurshid Fang Liu Robert Hasterok Xinlian Shen Kai Wang Source Type: research
Characterization of centromeric DNA of Gossypium anomalum reveals sequence-independent enrichment dynamics of centromeric repeats
Chromosome Res. 2023 Mar 27;31(2):12. doi: 10.1007/s10577-023-09721-z.ABSTRACTCentromeres in eukaryotes are composed of highly repetitive DNAs, which evolve rapidly and are thought to achieve a favorable structure in mature centromeres. However, how the centromeric repeat evolves into an adaptive structure is largely unknown. We characterized the centromeric sequences of Gossypium anomalum through chromatin immunoprecipitation against CENH3 antibodies. We revealed that the G. anomalum centromeres contained only retrotransposon-like repeats but were depleted in long arrays of satellites. These retrotransposon-like centromer...
Source: Chromosome Research - March 27, 2023 Category: Genetics & Stem Cells Authors: Wenjie Ding Yuanbin Zhu Jinlei Han Hui Zhang Zhenzhen Xu Haris Khurshid Fang Liu Robert Hasterok Xinlian Shen Kai Wang Source Type: research
Characterization of centromeric DNA of Gossypium anomalum reveals sequence-independent enrichment dynamics of centromeric repeats
Chromosome Res. 2023 Mar 27;31(2):12. doi: 10.1007/s10577-023-09721-z.ABSTRACTCentromeres in eukaryotes are composed of highly repetitive DNAs, which evolve rapidly and are thought to achieve a favorable structure in mature centromeres. However, how the centromeric repeat evolves into an adaptive structure is largely unknown. We characterized the centromeric sequences of Gossypium anomalum through chromatin immunoprecipitation against CENH3 antibodies. We revealed that the G. anomalum centromeres contained only retrotransposon-like repeats but were depleted in long arrays of satellites. These retrotransposon-like centromer...
Source: Chromosome Research - March 27, 2023 Category: Genetics & Stem Cells Authors: Wenjie Ding Yuanbin Zhu Jinlei Han Hui Zhang Zhenzhen Xu Haris Khurshid Fang Liu Robert Hasterok Xinlian Shen Kai Wang Source Type: research
Characterization of centromeric DNA of Gossypium anomalum reveals sequence-independent enrichment dynamics of centromeric repeats
Chromosome Res. 2023 Mar 27;31(2):12. doi: 10.1007/s10577-023-09721-z.ABSTRACTCentromeres in eukaryotes are composed of highly repetitive DNAs, which evolve rapidly and are thought to achieve a favorable structure in mature centromeres. However, how the centromeric repeat evolves into an adaptive structure is largely unknown. We characterized the centromeric sequences of Gossypium anomalum through chromatin immunoprecipitation against CENH3 antibodies. We revealed that the G. anomalum centromeres contained only retrotransposon-like repeats but were depleted in long arrays of satellites. These retrotransposon-like centromer...
Source: Chromosome Research - March 27, 2023 Category: Genetics & Stem Cells Authors: Wenjie Ding Yuanbin Zhu Jinlei Han Hui Zhang Zhenzhen Xu Haris Khurshid Fang Liu Robert Hasterok Xinlian Shen Kai Wang Source Type: research
Characterization of centromeric DNA of Gossypium anomalum reveals sequence-independent enrichment dynamics of centromeric repeats
Chromosome Res. 2023 Mar 27;31(2):12. doi: 10.1007/s10577-023-09721-z.ABSTRACTCentromeres in eukaryotes are composed of highly repetitive DNAs, which evolve rapidly and are thought to achieve a favorable structure in mature centromeres. However, how the centromeric repeat evolves into an adaptive structure is largely unknown. We characterized the centromeric sequences of Gossypium anomalum through chromatin immunoprecipitation against CENH3 antibodies. We revealed that the G. anomalum centromeres contained only retrotransposon-like repeats but were depleted in long arrays of satellites. These retrotransposon-like centromer...
Source: Chromosome Research - March 27, 2023 Category: Genetics & Stem Cells Authors: Wenjie Ding Yuanbin Zhu Jinlei Han Hui Zhang Zhenzhen Xu Haris Khurshid Fang Liu Robert Hasterok Xinlian Shen Kai Wang Source Type: research
Characterization of centromeric DNA of Gossypium anomalum reveals sequence-independent enrichment dynamics of centromeric repeats
Chromosome Res. 2023 Mar 27;31(2):12. doi: 10.1007/s10577-023-09721-z.ABSTRACTCentromeres in eukaryotes are composed of highly repetitive DNAs, which evolve rapidly and are thought to achieve a favorable structure in mature centromeres. However, how the centromeric repeat evolves into an adaptive structure is largely unknown. We characterized the centromeric sequences of Gossypium anomalum through chromatin immunoprecipitation against CENH3 antibodies. We revealed that the G. anomalum centromeres contained only retrotransposon-like repeats but were depleted in long arrays of satellites. These retrotransposon-like centromer...
Source: Chromosome Research - March 27, 2023 Category: Genetics & Stem Cells Authors: Wenjie Ding Yuanbin Zhu Jinlei Han Hui Zhang Zhenzhen Xu Haris Khurshid Fang Liu Robert Hasterok Xinlian Shen Kai Wang Source Type: research
Characterization of centromeric DNA of Gossypium anomalum reveals sequence-independent enrichment dynamics of centromeric repeats
Chromosome Res. 2023 Mar 27;31(2):12. doi: 10.1007/s10577-023-09721-z.ABSTRACTCentromeres in eukaryotes are composed of highly repetitive DNAs, which evolve rapidly and are thought to achieve a favorable structure in mature centromeres. However, how the centromeric repeat evolves into an adaptive structure is largely unknown. We characterized the centromeric sequences of Gossypium anomalum through chromatin immunoprecipitation against CENH3 antibodies. We revealed that the G. anomalum centromeres contained only retrotransposon-like repeats but were depleted in long arrays of satellites. These retrotransposon-like centromer...
Source: Chromosome Research - March 27, 2023 Category: Genetics & Stem Cells Authors: Wenjie Ding Yuanbin Zhu Jinlei Han Hui Zhang Zhenzhen Xu Haris Khurshid Fang Liu Robert Hasterok Xinlian Shen Kai Wang Source Type: research
Characterization of centromeric DNA of Gossypium anomalum reveals sequence-independent enrichment dynamics of centromeric repeats
Chromosome Res. 2023 Mar 27;31(2):12. doi: 10.1007/s10577-023-09721-z.ABSTRACTCentromeres in eukaryotes are composed of highly repetitive DNAs, which evolve rapidly and are thought to achieve a favorable structure in mature centromeres. However, how the centromeric repeat evolves into an adaptive structure is largely unknown. We characterized the centromeric sequences of Gossypium anomalum through chromatin immunoprecipitation against CENH3 antibodies. We revealed that the G. anomalum centromeres contained only retrotransposon-like repeats but were depleted in long arrays of satellites. These retrotransposon-like centromer...
Source: Chromosome Research - March 27, 2023 Category: Genetics & Stem Cells Authors: Wenjie Ding Yuanbin Zhu Jinlei Han Hui Zhang Zhenzhen Xu Haris Khurshid Fang Liu Robert Hasterok Xinlian Shen Kai Wang Source Type: research
Characterization of centromeric DNA of Gossypium anomalum reveals sequence-independent enrichment dynamics of centromeric repeats
Chromosome Res. 2023 Mar 27;31(2):12. doi: 10.1007/s10577-023-09721-z.ABSTRACTCentromeres in eukaryotes are composed of highly repetitive DNAs, which evolve rapidly and are thought to achieve a favorable structure in mature centromeres. However, how the centromeric repeat evolves into an adaptive structure is largely unknown. We characterized the centromeric sequences of Gossypium anomalum through chromatin immunoprecipitation against CENH3 antibodies. We revealed that the G. anomalum centromeres contained only retrotransposon-like repeats but were depleted in long arrays of satellites. These retrotransposon-like centromer...
Source: Chromosome Research - March 27, 2023 Category: Genetics & Stem Cells Authors: Wenjie Ding Yuanbin Zhu Jinlei Han Hui Zhang Zhenzhen Xu Haris Khurshid Fang Liu Robert Hasterok Xinlian Shen Kai Wang Source Type: research
Characterization of centromeric DNA of Gossypium anomalum reveals sequence-independent enrichment dynamics of centromeric repeats
Chromosome Res. 2023 Mar 27;31(2):12. doi: 10.1007/s10577-023-09721-z.ABSTRACTCentromeres in eukaryotes are composed of highly repetitive DNAs, which evolve rapidly and are thought to achieve a favorable structure in mature centromeres. However, how the centromeric repeat evolves into an adaptive structure is largely unknown. We characterized the centromeric sequences of Gossypium anomalum through chromatin immunoprecipitation against CENH3 antibodies. We revealed that the G. anomalum centromeres contained only retrotransposon-like repeats but were depleted in long arrays of satellites. These retrotransposon-like centromer...
Source: Chromosome Research - March 27, 2023 Category: Genetics & Stem Cells Authors: Wenjie Ding Yuanbin Zhu Jinlei Han Hui Zhang Zhenzhen Xu Haris Khurshid Fang Liu Robert Hasterok Xinlian Shen Kai Wang Source Type: research
A Cre-LoxP-based approach for combinatorial chromosome rearrangements in human HAP1 cells
Chromosome Res. 2023 Feb 26;31(1):11. doi: 10.1007/s10577-023-09719-7.ABSTRACTAlterations of human karyotype caused by chromosomal rearrangements are often associated with considerable phenotypic effects. Studying molecular mechanisms underlying these effects requires an efficient and scalable experimental model. Here, we propose a Cre-LoxP-based approach for the generation of combinatorial diversity of chromosomal rearrangements. We demonstrate that using the developed system, both intra- and inter-chromosomal rearrangements can be induced in the human haploid HAP1 cells, although the latter is significantly less effectiv...
Source: Chromosome Research - February 26, 2023 Category: Genetics & Stem Cells Authors: Anna Khabarova Galina Koksharova Pavel Salnikov Polina Belokopytova Roman Mungalov Inna Pristyazhnuk Artem Nurislamov Maria Gridina Veniamin Fishman Source Type: research
