A Cre-LoxP-based approach for combinatorial chromosome rearrangements in human HAP1 cells

Chromosome Res. 2023 Feb 26;31(1):11. doi: 10.1007/s10577-023-09719-7.ABSTRACTAlterations of human karyotype caused by chromosomal rearrangements are often associated with considerable phenotypic effects. Studying molecular mechanisms underlying these effects requires an efficient and scalable experimental model. Here, we propose a Cre-LoxP-based approach for the generation of combinatorial diversity of chromosomal rearrangements. We demonstrate that using the developed system, both intra- and inter-chromosomal rearrangements can be induced in the human haploid HAP1 cells, although the latter is significantly less effective. The obtained genetically modified HAP1 cell line can be used to dissect genomic effects associated with intra-chromosomal structural variations.PMID:36842155 | DOI:10.1007/s10577-023-09719-7

Source: Chromosome Research - February 26, 2023 Category: Genetics & Stem Cells Authors: Anna Khabarova Galina Koksharova Pavel Salnikov Polina Belokopytova Roman Mungalov Inna Pristyazhnuk Artem Nurislamov Maria Gridina Veniamin Fishman Source Type: research