Disentangling the roles of aneuploidy, chromosomal instability and tumour heterogeneity in developing resistance to cancer therapies
Chromosome Res. 2023 Sep 18;31(4):28. doi: 10.1007/s10577-023-09737-5.ABSTRACTAneuploidy is defined as the cellular state of having a number of chromosomes that deviates from a multiple of the normal haploid chromosome number of a given organism. Aneuploidy can be present in a static state: Down syndrome individuals stably maintain an extra copy of chromosome 21 in their cells. In cancer cells, however, aneuploidy is usually present in combination with chromosomal instability (CIN) which leads to a continual generation of new chromosomal alterations and the development of intratumour heterogeneity (ITH). The prevalence of ...
Source: Chromosome Research - September 18, 2023 Category: Genetics & Stem Cells Authors: Joana Reis Andrade Annie Dinky Gallagher Jovanna Maharaj Sarah Elizabeth McClelland Source Type: research
Disentangling the roles of aneuploidy, chromosomal instability and tumour heterogeneity in developing resistance to cancer therapies
Chromosome Res. 2023 Sep 18;31(4):28. doi: 10.1007/s10577-023-09737-5.ABSTRACTAneuploidy is defined as the cellular state of having a number of chromosomes that deviates from a multiple of the normal haploid chromosome number of a given organism. Aneuploidy can be present in a static state: Down syndrome individuals stably maintain an extra copy of chromosome 21 in their cells. In cancer cells, however, aneuploidy is usually present in combination with chromosomal instability (CIN) which leads to a continual generation of new chromosomal alterations and the development of intratumour heterogeneity (ITH). The prevalence of ...
Source: Chromosome Research - September 18, 2023 Category: Genetics & Stem Cells Authors: Joana Reis Andrade Annie Dinky Gallagher Jovanna Maharaj Sarah Elizabeth McClelland Source Type: research
Disentangling the roles of aneuploidy, chromosomal instability and tumour heterogeneity in developing resistance to cancer therapies
Chromosome Res. 2023 Sep 18;31(4):28. doi: 10.1007/s10577-023-09737-5.ABSTRACTAneuploidy is defined as the cellular state of having a number of chromosomes that deviates from a multiple of the normal haploid chromosome number of a given organism. Aneuploidy can be present in a static state: Down syndrome individuals stably maintain an extra copy of chromosome 21 in their cells. In cancer cells, however, aneuploidy is usually present in combination with chromosomal instability (CIN) which leads to a continual generation of new chromosomal alterations and the development of intratumour heterogeneity (ITH). The prevalence of ...
Source: Chromosome Research - September 18, 2023 Category: Genetics & Stem Cells Authors: Joana Reis Andrade Annie Dinky Gallagher Jovanna Maharaj Sarah Elizabeth McClelland Source Type: research
Disentangling the roles of aneuploidy, chromosomal instability and tumour heterogeneity in developing resistance to cancer therapies
Chromosome Res. 2023 Sep 18;31(4):28. doi: 10.1007/s10577-023-09737-5.ABSTRACTAneuploidy is defined as the cellular state of having a number of chromosomes that deviates from a multiple of the normal haploid chromosome number of a given organism. Aneuploidy can be present in a static state: Down syndrome individuals stably maintain an extra copy of chromosome 21 in their cells. In cancer cells, however, aneuploidy is usually present in combination with chromosomal instability (CIN) which leads to a continual generation of new chromosomal alterations and the development of intratumour heterogeneity (ITH). The prevalence of ...
Source: Chromosome Research - September 18, 2023 Category: Genetics & Stem Cells Authors: Joana Reis Andrade Annie Dinky Gallagher Jovanna Maharaj Sarah Elizabeth McClelland Source Type: research
Disentangling the roles of aneuploidy, chromosomal instability and tumour heterogeneity in developing resistance to cancer therapies
Chromosome Res. 2023 Sep 18;31(4):28. doi: 10.1007/s10577-023-09737-5.ABSTRACTAneuploidy is defined as the cellular state of having a number of chromosomes that deviates from a multiple of the normal haploid chromosome number of a given organism. Aneuploidy can be present in a static state: Down syndrome individuals stably maintain an extra copy of chromosome 21 in their cells. In cancer cells, however, aneuploidy is usually present in combination with chromosomal instability (CIN) which leads to a continual generation of new chromosomal alterations and the development of intratumour heterogeneity (ITH). The prevalence of ...
Source: Chromosome Research - September 18, 2023 Category: Genetics & Stem Cells Authors: Joana Reis Andrade Annie Dinky Gallagher Jovanna Maharaj Sarah Elizabeth McClelland Source Type: research
Disentangling the roles of aneuploidy, chromosomal instability and tumour heterogeneity in developing resistance to cancer therapies
Chromosome Res. 2023 Sep 18;31(4):28. doi: 10.1007/s10577-023-09737-5.ABSTRACTAneuploidy is defined as the cellular state of having a number of chromosomes that deviates from a multiple of the normal haploid chromosome number of a given organism. Aneuploidy can be present in a static state: Down syndrome individuals stably maintain an extra copy of chromosome 21 in their cells. In cancer cells, however, aneuploidy is usually present in combination with chromosomal instability (CIN) which leads to a continual generation of new chromosomal alterations and the development of intratumour heterogeneity (ITH). The prevalence of ...
Source: Chromosome Research - September 18, 2023 Category: Genetics & Stem Cells Authors: Joana Reis Andrade Annie Dinky Gallagher Jovanna Maharaj Sarah Elizabeth McClelland Source Type: research
Disentangling the roles of aneuploidy, chromosomal instability and tumour heterogeneity in developing resistance to cancer therapies
Chromosome Res. 2023 Sep 18;31(4):28. doi: 10.1007/s10577-023-09737-5.ABSTRACTAneuploidy is defined as the cellular state of having a number of chromosomes that deviates from a multiple of the normal haploid chromosome number of a given organism. Aneuploidy can be present in a static state: Down syndrome individuals stably maintain an extra copy of chromosome 21 in their cells. In cancer cells, however, aneuploidy is usually present in combination with chromosomal instability (CIN) which leads to a continual generation of new chromosomal alterations and the development of intratumour heterogeneity (ITH). The prevalence of ...
Source: Chromosome Research - September 18, 2023 Category: Genetics & Stem Cells Authors: Joana Reis Andrade Annie Dinky Gallagher Jovanna Maharaj Sarah Elizabeth McClelland Source Type: research
Disentangling the roles of aneuploidy, chromosomal instability and tumour heterogeneity in developing resistance to cancer therapies
Chromosome Res. 2023 Sep 18;31(4):28. doi: 10.1007/s10577-023-09737-5.ABSTRACTAneuploidy is defined as the cellular state of having a number of chromosomes that deviates from a multiple of the normal haploid chromosome number of a given organism. Aneuploidy can be present in a static state: Down syndrome individuals stably maintain an extra copy of chromosome 21 in their cells. In cancer cells, however, aneuploidy is usually present in combination with chromosomal instability (CIN) which leads to a continual generation of new chromosomal alterations and the development of intratumour heterogeneity (ITH). The prevalence of ...
Source: Chromosome Research - September 18, 2023 Category: Genetics & Stem Cells Authors: Joana Reis Andrade Annie Dinky Gallagher Jovanna Maharaj Sarah Elizabeth McClelland Source Type: research
Analog-sensitive Cdk1 as a tool to study mitotic exit: protein phosphatase 1 is required downstream from Cdk1 inactivation in budding yeast
Chromosome Res. 2023 Sep 10;31(3):27. doi: 10.1007/s10577-023-09736-6.ABSTRACTWe show that specific inactivation of the protein kinase Cdk1/cyclin B (Cdc28/Clb2) triggers exit from mitosis in the budding yeast Saccharomyces cerevisiae. Cells carrying the allele cdc28-as1, which makes Cdk1 (Cdc28) uniquely sensitive to the ATP analog 1NM-PP1, were arrested with spindle poisons and then treated with 1NM-PP1 to inhibit Cdk1. This caused the cells to leave mitosis and enter G1-phase as shown by initiation of rebudding (without cytokinesis), induction of mating projections ("shmoos") by α-factor, stabilization of Sic1, and deg...
Source: Chromosome Research - September 10, 2023 Category: Genetics & Stem Cells Authors: Jason M Keaton Benjamin G Workman Linfeng Xie James R Paulson Source Type: research
Analog-sensitive Cdk1 as a tool to study mitotic exit: protein phosphatase 1 is required downstream from Cdk1 inactivation in budding yeast
Chromosome Res. 2023 Sep 10;31(3):27. doi: 10.1007/s10577-023-09736-6.ABSTRACTWe show that specific inactivation of the protein kinase Cdk1/cyclin B (Cdc28/Clb2) triggers exit from mitosis in the budding yeast Saccharomyces cerevisiae. Cells carrying the allele cdc28-as1, which makes Cdk1 (Cdc28) uniquely sensitive to the ATP analog 1NM-PP1, were arrested with spindle poisons and then treated with 1NM-PP1 to inhibit Cdk1. This caused the cells to leave mitosis and enter G1-phase as shown by initiation of rebudding (without cytokinesis), induction of mating projections ("shmoos") by α-factor, stabilization of Sic1, and deg...
Source: Chromosome Research - September 10, 2023 Category: Genetics & Stem Cells Authors: Jason M Keaton Benjamin G Workman Linfeng Xie James R Paulson Source Type: research
Analog-sensitive Cdk1 as a tool to study mitotic exit: protein phosphatase 1 is required downstream from Cdk1 inactivation in budding yeast
Chromosome Res. 2023 Sep 10;31(3):27. doi: 10.1007/s10577-023-09736-6.ABSTRACTWe show that specific inactivation of the protein kinase Cdk1/cyclin B (Cdc28/Clb2) triggers exit from mitosis in the budding yeast Saccharomyces cerevisiae. Cells carrying the allele cdc28-as1, which makes Cdk1 (Cdc28) uniquely sensitive to the ATP analog 1NM-PP1, were arrested with spindle poisons and then treated with 1NM-PP1 to inhibit Cdk1. This caused the cells to leave mitosis and enter G1-phase as shown by initiation of rebudding (without cytokinesis), induction of mating projections ("shmoos") by α-factor, stabilization of Sic1, and deg...
Source: Chromosome Research - September 10, 2023 Category: Genetics & Stem Cells Authors: Jason M Keaton Benjamin G Workman Linfeng Xie James R Paulson Source Type: research
Analog-sensitive Cdk1 as a tool to study mitotic exit: protein phosphatase 1 is required downstream from Cdk1 inactivation in budding yeast
Chromosome Res. 2023 Sep 10;31(3):27. doi: 10.1007/s10577-023-09736-6.ABSTRACTWe show that specific inactivation of the protein kinase Cdk1/cyclin B (Cdc28/Clb2) triggers exit from mitosis in the budding yeast Saccharomyces cerevisiae. Cells carrying the allele cdc28-as1, which makes Cdk1 (Cdc28) uniquely sensitive to the ATP analog 1NM-PP1, were arrested with spindle poisons and then treated with 1NM-PP1 to inhibit Cdk1. This caused the cells to leave mitosis and enter G1-phase as shown by initiation of rebudding (without cytokinesis), induction of mating projections ("shmoos") by α-factor, stabilization of Sic1, and deg...
Source: Chromosome Research - September 10, 2023 Category: Genetics & Stem Cells Authors: Jason M Keaton Benjamin G Workman Linfeng Xie James R Paulson Source Type: research
Analog-sensitive Cdk1 as a tool to study mitotic exit: protein phosphatase 1 is required downstream from Cdk1 inactivation in budding yeast
Chromosome Res. 2023 Sep 10;31(3):27. doi: 10.1007/s10577-023-09736-6.ABSTRACTWe show that specific inactivation of the protein kinase Cdk1/cyclin B (Cdc28/Clb2) triggers exit from mitosis in the budding yeast Saccharomyces cerevisiae. Cells carrying the allele cdc28-as1, which makes Cdk1 (Cdc28) uniquely sensitive to the ATP analog 1NM-PP1, were arrested with spindle poisons and then treated with 1NM-PP1 to inhibit Cdk1. This caused the cells to leave mitosis and enter G1-phase as shown by initiation of rebudding (without cytokinesis), induction of mating projections ("shmoos") by α-factor, stabilization of Sic1, and deg...
Source: Chromosome Research - September 10, 2023 Category: Genetics & Stem Cells Authors: Jason M Keaton Benjamin G Workman Linfeng Xie James R Paulson Source Type: research
Analog-sensitive Cdk1 as a tool to study mitotic exit: protein phosphatase 1 is required downstream from Cdk1 inactivation in budding yeast
Chromosome Res. 2023 Sep 10;31(3):27. doi: 10.1007/s10577-023-09736-6.ABSTRACTWe show that specific inactivation of the protein kinase Cdk1/cyclin B (Cdc28/Clb2) triggers exit from mitosis in the budding yeast Saccharomyces cerevisiae. Cells carrying the allele cdc28-as1, which makes Cdk1 (Cdc28) uniquely sensitive to the ATP analog 1NM-PP1, were arrested with spindle poisons and then treated with 1NM-PP1 to inhibit Cdk1. This caused the cells to leave mitosis and enter G1-phase as shown by initiation of rebudding (without cytokinesis), induction of mating projections ("shmoos") by α-factor, stabilization of Sic1, and deg...
Source: Chromosome Research - September 10, 2023 Category: Genetics & Stem Cells Authors: Jason M Keaton Benjamin G Workman Linfeng Xie James R Paulson Source Type: research
Meiotic segregation and post-meiotic drive of the Festuca pratensis B chromosome
Chromosome Res. 2023 Sep 2;31(3):26. doi: 10.1007/s10577-023-09728-6.ABSTRACTIn many species, the transmission of B chromosomes (Bs) does not follow the Mendelian laws of equal segregation and independent assortment. This deviation results in transmission rates of Bs higher than 0.5, a process known as "chromosome drive". Here, we studied the behavior of the 103 Mbp-large B chromosome of Festuca pratensis during all meiotic and mitotic stages of microsporogenesis. Mostly, the B chromosome of F. pratensis segregates during meiosis like standard A chromosomes (As). In some cases, the B passes through meiosis in a non-Mendeli...
Source: Chromosome Research - September 2, 2023 Category: Genetics & Stem Cells Authors: Rahman Ebrahimzadegan J örg Fuchs Jianyong Chen Veit Schubert Armin Meister Andreas Houben Ghader Mirzaghaderi Source Type: research
