Subventricular zone stem cell niche injury is associated with intestinal perforation in preterm infants and predicts future motor impairment
Cell Stem Cell. 2024 Mar 22:S1934-5909(24)00082-1. doi: 10.1016/j.stem.2024.03.001. Online ahead of print.ABSTRACTBrain injury is highly associated with preterm birth. Complications of prematurity, including spontaneous or necrotizing enterocolitis (NEC)-associated intestinal perforations, are linked to lifelong neurologic impairment, yet the mechanisms are poorly understood. Early diagnosis of preterm brain injuries remains a significant challenge. Here, we identified subventricular zone echogenicity (SVE) on cranial ultrasound in preterm infants following intestinal perforations. The development of SVE was significantly ...
Source: Cell Stem Cell - March 27, 2024 Category: Stem Cells Authors: Adrian A Epstein Sara N Janos Luca Menozzi Kelly Pegram Vaibhav Jain Logan C Bisset Joseph T Davis Samantha Morrison Aswathy Shailaja Yingqiu Guo Agnes S Chao Khadar Abdi Blaire Rikard Junjie Yao Simon G Gregory Kimberley Fisher Rick Pittman Al Erkanli Ka Source Type: research
Subventricular zone stem cell niche injury is associated with intestinal perforation in preterm infants and predicts future motor impairment
Cell Stem Cell. 2024 Mar 22:S1934-5909(24)00082-1. doi: 10.1016/j.stem.2024.03.001. Online ahead of print.ABSTRACTBrain injury is highly associated with preterm birth. Complications of prematurity, including spontaneous or necrotizing enterocolitis (NEC)-associated intestinal perforations, are linked to lifelong neurologic impairment, yet the mechanisms are poorly understood. Early diagnosis of preterm brain injuries remains a significant challenge. Here, we identified subventricular zone echogenicity (SVE) on cranial ultrasound in preterm infants following intestinal perforations. The development of SVE was significantly ...
Source: Cell Stem Cell - March 27, 2024 Category: Stem Cells Authors: Adrian A Epstein Sara N Janos Luca Menozzi Kelly Pegram Vaibhav Jain Logan C Bisset Joseph T Davis Samantha Morrison Aswathy Shailaja Yingqiu Guo Agnes S Chao Khadar Abdi Blaire Rikard Junjie Yao Simon G Gregory Kimberley Fisher Rick Pittman Al Erkanli Ka Source Type: research
Subventricular zone stem cell niche injury is associated with intestinal perforation in preterm infants and predicts future motor impairment
Cell Stem Cell. 2024 Mar 22:S1934-5909(24)00082-1. doi: 10.1016/j.stem.2024.03.001. Online ahead of print.ABSTRACTBrain injury is highly associated with preterm birth. Complications of prematurity, including spontaneous or necrotizing enterocolitis (NEC)-associated intestinal perforations, are linked to lifelong neurologic impairment, yet the mechanisms are poorly understood. Early diagnosis of preterm brain injuries remains a significant challenge. Here, we identified subventricular zone echogenicity (SVE) on cranial ultrasound in preterm infants following intestinal perforations. The development of SVE was significantly ...
Source: Cell Stem Cell - March 27, 2024 Category: Stem Cells Authors: Adrian A Epstein Sara N Janos Luca Menozzi Kelly Pegram Vaibhav Jain Logan C Bisset Joseph T Davis Samantha Morrison Aswathy Shailaja Yingqiu Guo Agnes S Chao Khadar Abdi Blaire Rikard Junjie Yao Simon G Gregory Kimberley Fisher Rick Pittman Al Erkanli Ka Source Type: research
Subventricular zone stem cell niche injury is associated with intestinal perforation in preterm infants and predicts future motor impairment
Cell Stem Cell. 2024 Mar 22:S1934-5909(24)00082-1. doi: 10.1016/j.stem.2024.03.001. Online ahead of print.ABSTRACTBrain injury is highly associated with preterm birth. Complications of prematurity, including spontaneous or necrotizing enterocolitis (NEC)-associated intestinal perforations, are linked to lifelong neurologic impairment, yet the mechanisms are poorly understood. Early diagnosis of preterm brain injuries remains a significant challenge. Here, we identified subventricular zone echogenicity (SVE) on cranial ultrasound in preterm infants following intestinal perforations. The development of SVE was significantly ...
Source: Cell Stem Cell - March 27, 2024 Category: Stem Cells Authors: Adrian A Epstein Sara N Janos Luca Menozzi Kelly Pegram Vaibhav Jain Logan C Bisset Joseph T Davis Samantha Morrison Aswathy Shailaja Yingqiu Guo Agnes S Chao Khadar Abdi Blaire Rikard Junjie Yao Simon G Gregory Kimberley Fisher Rick Pittman Al Erkanli Ka Source Type: research
Subventricular zone stem cell niche injury is associated with intestinal perforation in preterm infants and predicts future motor impairment
Cell Stem Cell. 2024 Mar 22:S1934-5909(24)00082-1. doi: 10.1016/j.stem.2024.03.001. Online ahead of print.ABSTRACTBrain injury is highly associated with preterm birth. Complications of prematurity, including spontaneous or necrotizing enterocolitis (NEC)-associated intestinal perforations, are linked to lifelong neurologic impairment, yet the mechanisms are poorly understood. Early diagnosis of preterm brain injuries remains a significant challenge. Here, we identified subventricular zone echogenicity (SVE) on cranial ultrasound in preterm infants following intestinal perforations. The development of SVE was significantly ...
Source: Cell Stem Cell - March 27, 2024 Category: Stem Cells Authors: Adrian A Epstein Sara N Janos Luca Menozzi Kelly Pegram Vaibhav Jain Logan C Bisset Joseph T Davis Samantha Morrison Aswathy Shailaja Yingqiu Guo Agnes S Chao Khadar Abdi Blaire Rikard Junjie Yao Simon G Gregory Kimberley Fisher Rick Pittman Al Erkanli Ka Source Type: research
Autofluorescence is a biomarker of neural stem cell activation state
Cell Stem Cell. 2024 Mar 13:S1934-5909(24)00054-7. doi: 10.1016/j.stem.2024.02.011. Online ahead of print.ABSTRACTNeural stem cells (NSCs) must exit quiescence to produce neurons; however, our understanding of this process remains constrained by the technical limitations of current technologies. Fluorescence lifetime imaging (FLIM) of autofluorescent metabolic cofactors has been used in other cell types to study shifts in cell states driven by metabolic remodeling that change the optical properties of these endogenous fluorophores. Using this non-destructive, live-cell, and label-free strategy, we found that quiescent NSCs...
Source: Cell Stem Cell - March 23, 2024 Category: Stem Cells Authors: Christopher S Morrow Kelsey Tweed Sabina Farhadova Alex J Walsh Bo P Lear Avtar Roopra Ryan D Risgaard Payton C Klosa Zachary P Arndt Ella R Peterson Michelle M Chi Allison G Harris Melissa C Skala Darcie L Moore Source Type: research
Autofluorescence is a biomarker of neural stem cell activation state
Cell Stem Cell. 2024 Mar 13:S1934-5909(24)00054-7. doi: 10.1016/j.stem.2024.02.011. Online ahead of print.ABSTRACTNeural stem cells (NSCs) must exit quiescence to produce neurons; however, our understanding of this process remains constrained by the technical limitations of current technologies. Fluorescence lifetime imaging (FLIM) of autofluorescent metabolic cofactors has been used in other cell types to study shifts in cell states driven by metabolic remodeling that change the optical properties of these endogenous fluorophores. Using this non-destructive, live-cell, and label-free strategy, we found that quiescent NSCs...
Source: Cell Stem Cell - March 23, 2024 Category: Stem Cells Authors: Christopher S Morrow Kelsey Tweed Sabina Farhadova Alex J Walsh Bo P Lear Avtar Roopra Ryan D Risgaard Payton C Klosa Zachary P Arndt Ella R Peterson Michelle M Chi Allison G Harris Melissa C Skala Darcie L Moore Source Type: research
Autofluorescence is a biomarker of neural stem cell activation state
Cell Stem Cell. 2024 Mar 13:S1934-5909(24)00054-7. doi: 10.1016/j.stem.2024.02.011. Online ahead of print.ABSTRACTNeural stem cells (NSCs) must exit quiescence to produce neurons; however, our understanding of this process remains constrained by the technical limitations of current technologies. Fluorescence lifetime imaging (FLIM) of autofluorescent metabolic cofactors has been used in other cell types to study shifts in cell states driven by metabolic remodeling that change the optical properties of these endogenous fluorophores. Using this non-destructive, live-cell, and label-free strategy, we found that quiescent NSCs...
Source: Cell Stem Cell - March 23, 2024 Category: Stem Cells Authors: Christopher S Morrow Kelsey Tweed Sabina Farhadova Alex J Walsh Bo P Lear Avtar Roopra Ryan D Risgaard Payton C Klosa Zachary P Arndt Ella R Peterson Michelle M Chi Allison G Harris Melissa C Skala Darcie L Moore Source Type: research
Autofluorescence is a biomarker of neural stem cell activation state
Cell Stem Cell. 2024 Mar 13:S1934-5909(24)00054-7. doi: 10.1016/j.stem.2024.02.011. Online ahead of print.ABSTRACTNeural stem cells (NSCs) must exit quiescence to produce neurons; however, our understanding of this process remains constrained by the technical limitations of current technologies. Fluorescence lifetime imaging (FLIM) of autofluorescent metabolic cofactors has been used in other cell types to study shifts in cell states driven by metabolic remodeling that change the optical properties of these endogenous fluorophores. Using this non-destructive, live-cell, and label-free strategy, we found that quiescent NSCs...
Source: Cell Stem Cell - March 23, 2024 Category: Stem Cells Authors: Christopher S Morrow Kelsey Tweed Sabina Farhadova Alex J Walsh Bo P Lear Avtar Roopra Ryan D Risgaard Payton C Klosa Zachary P Arndt Ella R Peterson Michelle M Chi Allison G Harris Melissa C Skala Darcie L Moore Source Type: research
Impact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques
Cell Stem Cell. 2024 Mar 12:S1934-5909(24)00053-5. doi: 10.1016/j.stem.2024.02.010. Online ahead of print.ABSTRACTFor precise genome editing via CRISPR/homology-directed repair (HDR), effective and safe editing of long-term engrafting hematopoietic stem cells (LT-HSCs) is required. The impact of HDR on true LT-HSC clonal dynamics in a relevant large animal model has not been studied. To track the output and clonality of HDR-edited cells and to provide a comparison to lentivirally transduced HSCs in vivo, we developed a competitive rhesus macaque (RM) autologous transplantation model, co-infusing HSCs transduced with a barc...
Source: Cell Stem Cell - March 20, 2024 Category: Stem Cells Authors: Byung-Chul Lee Ashley Gin Chuanfeng Wu Komudi Singh Max Grice Ryland Mortlock Diana Abraham Xing Fan Yifan Zhou Aisha AlJanahi Uimook Choi Suk See DeRavin Taehoon Shin Sogun Hong Cynthia E Dunbar Source Type: research
Impact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques
Cell Stem Cell. 2024 Mar 12:S1934-5909(24)00053-5. doi: 10.1016/j.stem.2024.02.010. Online ahead of print.ABSTRACTFor precise genome editing via CRISPR/homology-directed repair (HDR), effective and safe editing of long-term engrafting hematopoietic stem cells (LT-HSCs) is required. The impact of HDR on true LT-HSC clonal dynamics in a relevant large animal model has not been studied. To track the output and clonality of HDR-edited cells and to provide a comparison to lentivirally transduced HSCs in vivo, we developed a competitive rhesus macaque (RM) autologous transplantation model, co-infusing HSCs transduced with a barc...
Source: Cell Stem Cell - March 20, 2024 Category: Stem Cells Authors: Byung-Chul Lee Ashley Gin Chuanfeng Wu Komudi Singh Max Grice Ryland Mortlock Diana Abraham Xing Fan Yifan Zhou Aisha AlJanahi Uimook Choi Suk See DeRavin Taehoon Shin Sogun Hong Cynthia E Dunbar Source Type: research
Impact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques
Cell Stem Cell. 2024 Mar 12:S1934-5909(24)00053-5. doi: 10.1016/j.stem.2024.02.010. Online ahead of print.ABSTRACTFor precise genome editing via CRISPR/homology-directed repair (HDR), effective and safe editing of long-term engrafting hematopoietic stem cells (LT-HSCs) is required. The impact of HDR on true LT-HSC clonal dynamics in a relevant large animal model has not been studied. To track the output and clonality of HDR-edited cells and to provide a comparison to lentivirally transduced HSCs in vivo, we developed a competitive rhesus macaque (RM) autologous transplantation model, co-infusing HSCs transduced with a barc...
Source: Cell Stem Cell - March 20, 2024 Category: Stem Cells Authors: Byung-Chul Lee Ashley Gin Chuanfeng Wu Komudi Singh Max Grice Ryland Mortlock Diana Abraham Xing Fan Yifan Zhou Aisha AlJanahi Uimook Choi Suk See DeRavin Taehoon Shin Sogun Hong Cynthia E Dunbar Source Type: research
Lung repair and regeneration: Advanced models and insights into human disease
Cell Stem Cell. 2024 Mar 11:S1934-5909(24)00052-3. doi: 10.1016/j.stem.2024.02.009. Online ahead of print.ABSTRACTThe respiratory system acts as both the primary site of gas exchange and an important sensor and barrier to the external environment. The increase in incidences of respiratory disease over the past decades has highlighted the importance of developing improved therapeutic approaches. This review will summarize recent research on the cellular complexity of the mammalian respiratory system with a focus on gas exchange and immunological defense functions of the lung. Different models of repair and regeneration will...
Source: Cell Stem Cell - March 16, 2024 Category: Stem Cells Authors: Maria C Basil Konstantinos-Dionysios Alysandratos Darrell N Kotton Edward E Morrisey Source Type: research
Lung repair and regeneration: Advanced models and insights into human disease
Cell Stem Cell. 2024 Mar 11:S1934-5909(24)00052-3. doi: 10.1016/j.stem.2024.02.009. Online ahead of print.ABSTRACTThe respiratory system acts as both the primary site of gas exchange and an important sensor and barrier to the external environment. The increase in incidences of respiratory disease over the past decades has highlighted the importance of developing improved therapeutic approaches. This review will summarize recent research on the cellular complexity of the mammalian respiratory system with a focus on gas exchange and immunological defense functions of the lung. Different models of repair and regeneration will...
Source: Cell Stem Cell - March 16, 2024 Category: Stem Cells Authors: Maria C Basil Konstantinos-Dionysios Alysandratos Darrell N Kotton Edward E Morrisey Source Type: research
Lung repair and regeneration: Advanced models and insights into human disease
Cell Stem Cell. 2024 Mar 11:S1934-5909(24)00052-3. doi: 10.1016/j.stem.2024.02.009. Online ahead of print.ABSTRACTThe respiratory system acts as both the primary site of gas exchange and an important sensor and barrier to the external environment. The increase in incidences of respiratory disease over the past decades has highlighted the importance of developing improved therapeutic approaches. This review will summarize recent research on the cellular complexity of the mammalian respiratory system with a focus on gas exchange and immunological defense functions of the lung. Different models of repair and regeneration will...
Source: Cell Stem Cell - March 16, 2024 Category: Stem Cells Authors: Maria C Basil Konstantinos-Dionysios Alysandratos Darrell N Kotton Edward E Morrisey Source Type: research
