Region-specific cellular and molecular basis of liver regeneration after acute pericentral injury
Cell Stem Cell. 2024 Feb 21:S1934-5909(24)00043-2. doi: 10.1016/j.stem.2024.01.013. Online ahead of print.ABSTRACTLiver injuries often occur in a zonated manner. However, detailed regenerative responses to such zonal injuries at cellular and molecular levels remain largely elusive. By using a fate-mapping strain, Cyp2e1-DreER, to elucidate liver regeneration after acute pericentral injury, we found that pericentral regeneration is primarily compensated by the expansion of remaining pericentral hepatocytes, and secondarily by expansion of periportal hepatocytes. Employing single-cell RNA sequencing, spatial transcriptomics,...
Source: Cell Stem Cell - February 25, 2024 Category: Stem Cells Authors: Shuyong Wang Xuan Wang Yiran Shan Zuolong Tan Yuxin Su Yannan Cao Shuang Wang Jiahong Dong Jin Gu Yunfang Wang Source Type: research
A human lymphoma organoid model for evaluating and targeting the follicular lymphoma tumor immune microenvironment
Cell Stem Cell. 2024 Feb 20:S1934-5909(24)00042-0. doi: 10.1016/j.stem.2024.01.012. Online ahead of print.ABSTRACTHeterogeneity in the tumor microenvironment (TME) of follicular lymphomas (FLs) can affect clinical outcomes. Current immunotherapeutic strategies, including antibody- and cell-based therapies, variably overcome pro-tumorigenic mechanisms for sustained disease control. Modeling the intact FL TME, with its native, syngeneic tumor-infiltrating leukocytes, is a major challenge. Here, we describe an organoid culture method for cultivating patient-derived lymphoma organoids (PDLOs), which include cells from the nati...
Source: Cell Stem Cell - February 25, 2024 Category: Stem Cells Authors: Jenna M Kastenschmidt Joseph G Schroers-Martin Brian J Sworder Suhas Sureshchandra Michael S Khodadoust Chih Long Liu Mari Olsen David M Kurtz Maximilian Diehn Lisa E Wagar Ash A Alizadeh Source Type: research
Thalamocortical organoids enable in vitro modeling of 22q11.2 microdeletion associated with neuropsychiatric disorders
Cell Stem Cell. 2024 Feb 13:S1934-5909(24)00040-7. doi: 10.1016/j.stem.2024.01.010. Online ahead of print.ABSTRACTThalamic dysfunction has been implicated in multiple psychiatric disorders. We sought to study the mechanisms by which abnormalities emerge in the context of the 22q11.2 microdeletion, which confers significant genetic risk for psychiatric disorders. We investigated early stages of human thalamus development using human pluripotent stem cell-derived organoids and show that the 22q11.2 microdeletion underlies widespread transcriptional dysregulation associated with psychiatric disorders in thalamic neurons and g...
Source: Cell Stem Cell - February 21, 2024 Category: Stem Cells Authors: David Shin Chang N Kim Jayden Ross Kelsey M Hennick Sih-Rong Wu Neha Paranjape Rachel Leonard Jerrick C Wang Matthew G Keefe Bryan J Pavlovic Kevin C Donohue Clara Moreau Emilie M Wigdor H Hanh Larson Denise E Allen Cathryn R Cadwell Aparna Bhaduri Galina Source Type: research
Hallmarks of totipotent and pluripotent stem cell states
Cell Stem Cell. 2024 Feb 13:S1934-5909(24)00039-0. doi: 10.1016/j.stem.2024.01.009. Online ahead of print.ABSTRACTThough totipotency and pluripotency are transient during early embryogenesis, they establish the foundation for the development of all mammals. Studying these in vivo has been challenging due to limited access and ethical constraints, particularly in humans. Recent progress has led to diverse culture adaptations of epiblast cells in vitro in the form of totipotent and pluripotent stem cells, which not only deepen our understanding of embryonic development but also serve as invaluable resources for animal reprod...
Source: Cell Stem Cell - February 21, 2024 Category: Stem Cells Authors: Peng Du Jun Wu Source Type: research
Thalamocortical organoids enable in vitro modeling of 22q11.2 microdeletion associated with neuropsychiatric disorders
Cell Stem Cell. 2024 Feb 13:S1934-5909(24)00040-7. doi: 10.1016/j.stem.2024.01.010. Online ahead of print.ABSTRACTThalamic dysfunction has been implicated in multiple psychiatric disorders. We sought to study the mechanisms by which abnormalities emerge in the context of the 22q11.2 microdeletion, which confers significant genetic risk for psychiatric disorders. We investigated early stages of human thalamus development using human pluripotent stem cell-derived organoids and show that the 22q11.2 microdeletion underlies widespread transcriptional dysregulation associated with psychiatric disorders in thalamic neurons and g...
Source: Cell Stem Cell - February 21, 2024 Category: Stem Cells Authors: David Shin Chang N Kim Jayden Ross Kelsey M Hennick Sih-Rong Wu Neha Paranjape Rachel Leonard Jerrick C Wang Matthew G Keefe Bryan J Pavlovic Kevin C Donohue Clara Moreau Emilie M Wigdor H Hanh Larson Denise E Allen Cathryn R Cadwell Aparna Bhaduri Galina Source Type: research
Hallmarks of totipotent and pluripotent stem cell states
Cell Stem Cell. 2024 Feb 13:S1934-5909(24)00039-0. doi: 10.1016/j.stem.2024.01.009. Online ahead of print.ABSTRACTThough totipotency and pluripotency are transient during early embryogenesis, they establish the foundation for the development of all mammals. Studying these in vivo has been challenging due to limited access and ethical constraints, particularly in humans. Recent progress has led to diverse culture adaptations of epiblast cells in vitro in the form of totipotent and pluripotent stem cells, which not only deepen our understanding of embryonic development but also serve as invaluable resources for animal reprod...
Source: Cell Stem Cell - February 21, 2024 Category: Stem Cells Authors: Peng Du Jun Wu Source Type: research
Thalamocortical organoids enable in vitro modeling of 22q11.2 microdeletion associated with neuropsychiatric disorders
Cell Stem Cell. 2024 Feb 13:S1934-5909(24)00040-7. doi: 10.1016/j.stem.2024.01.010. Online ahead of print.ABSTRACTThalamic dysfunction has been implicated in multiple psychiatric disorders. We sought to study the mechanisms by which abnormalities emerge in the context of the 22q11.2 microdeletion, which confers significant genetic risk for psychiatric disorders. We investigated early stages of human thalamus development using human pluripotent stem cell-derived organoids and show that the 22q11.2 microdeletion underlies widespread transcriptional dysregulation associated with psychiatric disorders in thalamic neurons and g...
Source: Cell Stem Cell - February 21, 2024 Category: Stem Cells Authors: David Shin Chang N Kim Jayden Ross Kelsey M Hennick Sih-Rong Wu Neha Paranjape Rachel Leonard Jerrick C Wang Matthew G Keefe Bryan J Pavlovic Kevin C Donohue Clara Moreau Emilie M Wigdor H Hanh Larson Denise E Allen Cathryn R Cadwell Aparna Bhaduri Galina Source Type: research
Hallmarks of totipotent and pluripotent stem cell states
Cell Stem Cell. 2024 Feb 13:S1934-5909(24)00039-0. doi: 10.1016/j.stem.2024.01.009. Online ahead of print.ABSTRACTThough totipotency and pluripotency are transient during early embryogenesis, they establish the foundation for the development of all mammals. Studying these in vivo has been challenging due to limited access and ethical constraints, particularly in humans. Recent progress has led to diverse culture adaptations of epiblast cells in vitro in the form of totipotent and pluripotent stem cells, which not only deepen our understanding of embryonic development but also serve as invaluable resources for animal reprod...
Source: Cell Stem Cell - February 21, 2024 Category: Stem Cells Authors: Peng Du Jun Wu Source Type: research
Thalamocortical organoids enable in vitro modeling of 22q11.2 microdeletion associated with neuropsychiatric disorders
Cell Stem Cell. 2024 Feb 13:S1934-5909(24)00040-7. doi: 10.1016/j.stem.2024.01.010. Online ahead of print.ABSTRACTThalamic dysfunction has been implicated in multiple psychiatric disorders. We sought to study the mechanisms by which abnormalities emerge in the context of the 22q11.2 microdeletion, which confers significant genetic risk for psychiatric disorders. We investigated early stages of human thalamus development using human pluripotent stem cell-derived organoids and show that the 22q11.2 microdeletion underlies widespread transcriptional dysregulation associated with psychiatric disorders in thalamic neurons and g...
Source: Cell Stem Cell - February 21, 2024 Category: Stem Cells Authors: David Shin Chang N Kim Jayden Ross Kelsey M Hennick Sih-Rong Wu Neha Paranjape Rachel Leonard Jerrick C Wang Matthew G Keefe Bryan J Pavlovic Kevin C Donohue Clara Moreau Emilie M Wigdor H Hanh Larson Denise E Allen Cathryn R Cadwell Aparna Bhaduri Galina Source Type: research
Hallmarks of totipotent and pluripotent stem cell states
Cell Stem Cell. 2024 Feb 13:S1934-5909(24)00039-0. doi: 10.1016/j.stem.2024.01.009. Online ahead of print.ABSTRACTThough totipotency and pluripotency are transient during early embryogenesis, they establish the foundation for the development of all mammals. Studying these in vivo has been challenging due to limited access and ethical constraints, particularly in humans. Recent progress has led to diverse culture adaptations of epiblast cells in vitro in the form of totipotent and pluripotent stem cells, which not only deepen our understanding of embryonic development but also serve as invaluable resources for animal reprod...
Source: Cell Stem Cell - February 21, 2024 Category: Stem Cells Authors: Peng Du Jun Wu Source Type: research
Strengthening cardiac therapy pipelines using human pluripotent stem cell-derived cardiomyocytes
Cell Stem Cell. 2024 Feb 12:S1934-5909(24)00008-0. doi: 10.1016/j.stem.2024.01.007. Online ahead of print.ABSTRACTAdvances in hiPSC isolation and reprogramming and hPSC-CM differentiation have prompted their therapeutic application and utilization for evaluating potential cardiovascular safety liabilities. In this perspective, we showcase key efforts toward the large-scale production of hiPSC-CMs, implementation of hiPSC-CMs in industry settings, and recent clinical applications of this technology. The key observations are a need for traceable gender and ethnically diverse hiPSC lines, approaches to reduce cost of scale-up...
Source: Cell Stem Cell - February 17, 2024 Category: Stem Cells Authors: Kavita Raniga Aishah Nasir Nguyen T N Vo Ravi Vaidyanathan Sarah Dickerson Simon Hilcove Diogo Mosqueira Gary R Mirams Peter Clements Ryan Hicks Amy Pointon Will Stebbeds Jo Francis Chris Denning Source Type: research
Canonical Wnt signaling directs the generation of functional human PSC-derived atrioventricular canal cardiomyocytes in bioprinted cardiac tissues
Cell Stem Cell. 2024 Feb 10:S1934-5909(24)00038-9. doi: 10.1016/j.stem.2024.01.008. Online ahead of print.ABSTRACTThe creation of a functional 3D bioprinted human heart remains challenging, largely due to the lack of some crucial cardiac cell types, including the atrioventricular canal (AVC) cardiomyocytes, which are essential to slow down the electrical impulse between the atrium and ventricle. By utilizing single-cell RNA sequencing analysis and a 3D bioprinting technology, we discover that stage-specific activation of canonical Wnt signaling creates functional AVC cardiomyocytes derived from human pluripotent stem cells...
Source: Cell Stem Cell - February 17, 2024 Category: Stem Cells Authors: Chenxi Ye Chuanlai Yang Heqiang Zhang Rui Gao Yingnan Liao Yali Zhang Lingjun Jie Yanhui Zhang Tong Cheng Yan Wang Jie Ren Source Type: research
Strengthening cardiac therapy pipelines using human pluripotent stem cell-derived cardiomyocytes
Cell Stem Cell. 2024 Feb 12:S1934-5909(24)00008-0. doi: 10.1016/j.stem.2024.01.007. Online ahead of print.ABSTRACTAdvances in hiPSC isolation and reprogramming and hPSC-CM differentiation have prompted their therapeutic application and utilization for evaluating potential cardiovascular safety liabilities. In this perspective, we showcase key efforts toward the large-scale production of hiPSC-CMs, implementation of hiPSC-CMs in industry settings, and recent clinical applications of this technology. The key observations are a need for traceable gender and ethnically diverse hiPSC lines, approaches to reduce cost of scale-up...
Source: Cell Stem Cell - February 17, 2024 Category: Stem Cells Authors: Kavita Raniga Aishah Nasir Nguyen T N Vo Ravi Vaidyanathan Sarah Dickerson Simon Hilcove Diogo Mosqueira Gary R Mirams Peter Clements Ryan Hicks Amy Pointon Will Stebbeds Jo Francis Chris Denning Source Type: research
Canonical Wnt signaling directs the generation of functional human PSC-derived atrioventricular canal cardiomyocytes in bioprinted cardiac tissues
Cell Stem Cell. 2024 Feb 10:S1934-5909(24)00038-9. doi: 10.1016/j.stem.2024.01.008. Online ahead of print.ABSTRACTThe creation of a functional 3D bioprinted human heart remains challenging, largely due to the lack of some crucial cardiac cell types, including the atrioventricular canal (AVC) cardiomyocytes, which are essential to slow down the electrical impulse between the atrium and ventricle. By utilizing single-cell RNA sequencing analysis and a 3D bioprinting technology, we discover that stage-specific activation of canonical Wnt signaling creates functional AVC cardiomyocytes derived from human pluripotent stem cells...
Source: Cell Stem Cell - February 17, 2024 Category: Stem Cells Authors: Chenxi Ye Chuanlai Yang Heqiang Zhang Rui Gao Yingnan Liao Yali Zhang Lingjun Jie Yanhui Zhang Tong Cheng Yan Wang Jie Ren Source Type: research
Strengthening cardiac therapy pipelines using human pluripotent stem cell-derived cardiomyocytes
Cell Stem Cell. 2024 Feb 12:S1934-5909(24)00008-0. doi: 10.1016/j.stem.2024.01.007. Online ahead of print.ABSTRACTAdvances in hiPSC isolation and reprogramming and hPSC-CM differentiation have prompted their therapeutic application and utilization for evaluating potential cardiovascular safety liabilities. In this perspective, we showcase key efforts toward the large-scale production of hiPSC-CMs, implementation of hiPSC-CMs in industry settings, and recent clinical applications of this technology. The key observations are a need for traceable gender and ethnically diverse hiPSC lines, approaches to reduce cost of scale-up...
Source: Cell Stem Cell - February 17, 2024 Category: Stem Cells Authors: Kavita Raniga Aishah Nasir Nguyen T N Vo Ravi Vaidyanathan Sarah Dickerson Simon Hilcove Diogo Mosqueira Gary R Mirams Peter Clements Ryan Hicks Amy Pointon Will Stebbeds Jo Francis Chris Denning Source Type: research
