Hallmarks of totipotent and pluripotent stem cell states
Cell Stem Cell. 2024 Feb 13:S1934-5909(24)00039-0. doi: 10.1016/j.stem.2024.01.009. Online ahead of print.ABSTRACTThough totipotency and pluripotency are transient during early embryogenesis, they establish the foundation for the development of all mammals. Studying these in vivo has been challenging due to limited access and ethical constraints, particularly in humans. Recent progress has led to diverse culture adaptations of epiblast cells in vitro in the form of totipotent and pluripotent stem cells, which not only deepen our understanding of embryonic development but also serve as invaluable resources for animal reprod...
Source: Cell Stem Cell - February 21, 2024 Category: Stem Cells Authors: Peng Du Jun Wu Source Type: research
Thalamocortical organoids enable in vitro modeling of 22q11.2 microdeletion associated with neuropsychiatric disorders
Cell Stem Cell. 2024 Feb 13:S1934-5909(24)00040-7. doi: 10.1016/j.stem.2024.01.010. Online ahead of print.ABSTRACTThalamic dysfunction has been implicated in multiple psychiatric disorders. We sought to study the mechanisms by which abnormalities emerge in the context of the 22q11.2 microdeletion, which confers significant genetic risk for psychiatric disorders. We investigated early stages of human thalamus development using human pluripotent stem cell-derived organoids and show that the 22q11.2 microdeletion underlies widespread transcriptional dysregulation associated with psychiatric disorders in thalamic neurons and g...
Source: Cell Stem Cell - February 21, 2024 Category: Stem Cells Authors: David Shin Chang N Kim Jayden Ross Kelsey M Hennick Sih-Rong Wu Neha Paranjape Rachel Leonard Jerrick C Wang Matthew G Keefe Bryan J Pavlovic Kevin C Donohue Clara Moreau Emilie M Wigdor H Hanh Larson Denise E Allen Cathryn R Cadwell Aparna Bhaduri Galina Source Type: research
Hallmarks of totipotent and pluripotent stem cell states
Cell Stem Cell. 2024 Feb 13:S1934-5909(24)00039-0. doi: 10.1016/j.stem.2024.01.009. Online ahead of print.ABSTRACTThough totipotency and pluripotency are transient during early embryogenesis, they establish the foundation for the development of all mammals. Studying these in vivo has been challenging due to limited access and ethical constraints, particularly in humans. Recent progress has led to diverse culture adaptations of epiblast cells in vitro in the form of totipotent and pluripotent stem cells, which not only deepen our understanding of embryonic development but also serve as invaluable resources for animal reprod...
Source: Cell Stem Cell - February 21, 2024 Category: Stem Cells Authors: Peng Du Jun Wu Source Type: research
Strengthening cardiac therapy pipelines using human pluripotent stem cell-derived cardiomyocytes
Cell Stem Cell. 2024 Feb 12:S1934-5909(24)00008-0. doi: 10.1016/j.stem.2024.01.007. Online ahead of print.ABSTRACTAdvances in hiPSC isolation and reprogramming and hPSC-CM differentiation have prompted their therapeutic application and utilization for evaluating potential cardiovascular safety liabilities. In this perspective, we showcase key efforts toward the large-scale production of hiPSC-CMs, implementation of hiPSC-CMs in industry settings, and recent clinical applications of this technology. The key observations are a need for traceable gender and ethnically diverse hiPSC lines, approaches to reduce cost of scale-up...
Source: Cell Stem Cell - February 17, 2024 Category: Stem Cells Authors: Kavita Raniga Aishah Nasir Nguyen T N Vo Ravi Vaidyanathan Sarah Dickerson Simon Hilcove Diogo Mosqueira Gary R Mirams Peter Clements Ryan Hicks Amy Pointon Will Stebbeds Jo Francis Chris Denning Source Type: research
Canonical Wnt signaling directs the generation of functional human PSC-derived atrioventricular canal cardiomyocytes in bioprinted cardiac tissues
Cell Stem Cell. 2024 Feb 10:S1934-5909(24)00038-9. doi: 10.1016/j.stem.2024.01.008. Online ahead of print.ABSTRACTThe creation of a functional 3D bioprinted human heart remains challenging, largely due to the lack of some crucial cardiac cell types, including the atrioventricular canal (AVC) cardiomyocytes, which are essential to slow down the electrical impulse between the atrium and ventricle. By utilizing single-cell RNA sequencing analysis and a 3D bioprinting technology, we discover that stage-specific activation of canonical Wnt signaling creates functional AVC cardiomyocytes derived from human pluripotent stem cells...
Source: Cell Stem Cell - February 17, 2024 Category: Stem Cells Authors: Chenxi Ye Chuanlai Yang Heqiang Zhang Rui Gao Yingnan Liao Yali Zhang Lingjun Jie Yanhui Zhang Tong Cheng Yan Wang Jie Ren Source Type: research
Strengthening cardiac therapy pipelines using human pluripotent stem cell-derived cardiomyocytes
Cell Stem Cell. 2024 Feb 12:S1934-5909(24)00008-0. doi: 10.1016/j.stem.2024.01.007. Online ahead of print.ABSTRACTAdvances in hiPSC isolation and reprogramming and hPSC-CM differentiation have prompted their therapeutic application and utilization for evaluating potential cardiovascular safety liabilities. In this perspective, we showcase key efforts toward the large-scale production of hiPSC-CMs, implementation of hiPSC-CMs in industry settings, and recent clinical applications of this technology. The key observations are a need for traceable gender and ethnically diverse hiPSC lines, approaches to reduce cost of scale-up...
Source: Cell Stem Cell - February 17, 2024 Category: Stem Cells Authors: Kavita Raniga Aishah Nasir Nguyen T N Vo Ravi Vaidyanathan Sarah Dickerson Simon Hilcove Diogo Mosqueira Gary R Mirams Peter Clements Ryan Hicks Amy Pointon Will Stebbeds Jo Francis Chris Denning Source Type: research
Canonical Wnt signaling directs the generation of functional human PSC-derived atrioventricular canal cardiomyocytes in bioprinted cardiac tissues
Cell Stem Cell. 2024 Feb 10:S1934-5909(24)00038-9. doi: 10.1016/j.stem.2024.01.008. Online ahead of print.ABSTRACTThe creation of a functional 3D bioprinted human heart remains challenging, largely due to the lack of some crucial cardiac cell types, including the atrioventricular canal (AVC) cardiomyocytes, which are essential to slow down the electrical impulse between the atrium and ventricle. By utilizing single-cell RNA sequencing analysis and a 3D bioprinting technology, we discover that stage-specific activation of canonical Wnt signaling creates functional AVC cardiomyocytes derived from human pluripotent stem cells...
Source: Cell Stem Cell - February 17, 2024 Category: Stem Cells Authors: Chenxi Ye Chuanlai Yang Heqiang Zhang Rui Gao Yingnan Liao Yali Zhang Lingjun Jie Yanhui Zhang Tong Cheng Yan Wang Jie Ren Source Type: research
Strengthening cardiac therapy pipelines using human pluripotent stem cell-derived cardiomyocytes
Cell Stem Cell. 2024 Feb 12:S1934-5909(24)00008-0. doi: 10.1016/j.stem.2024.01.007. Online ahead of print.ABSTRACTAdvances in hiPSC isolation and reprogramming and hPSC-CM differentiation have prompted their therapeutic application and utilization for evaluating potential cardiovascular safety liabilities. In this perspective, we showcase key efforts toward the large-scale production of hiPSC-CMs, implementation of hiPSC-CMs in industry settings, and recent clinical applications of this technology. The key observations are a need for traceable gender and ethnically diverse hiPSC lines, approaches to reduce cost of scale-up...
Source: Cell Stem Cell - February 17, 2024 Category: Stem Cells Authors: Kavita Raniga Aishah Nasir Nguyen T N Vo Ravi Vaidyanathan Sarah Dickerson Simon Hilcove Diogo Mosqueira Gary R Mirams Peter Clements Ryan Hicks Amy Pointon Will Stebbeds Jo Francis Chris Denning Source Type: research
Canonical Wnt signaling directs the generation of functional human PSC-derived atrioventricular canal cardiomyocytes in bioprinted cardiac tissues
Cell Stem Cell. 2024 Feb 10:S1934-5909(24)00038-9. doi: 10.1016/j.stem.2024.01.008. Online ahead of print.ABSTRACTThe creation of a functional 3D bioprinted human heart remains challenging, largely due to the lack of some crucial cardiac cell types, including the atrioventricular canal (AVC) cardiomyocytes, which are essential to slow down the electrical impulse between the atrium and ventricle. By utilizing single-cell RNA sequencing analysis and a 3D bioprinting technology, we discover that stage-specific activation of canonical Wnt signaling creates functional AVC cardiomyocytes derived from human pluripotent stem cells...
Source: Cell Stem Cell - February 17, 2024 Category: Stem Cells Authors: Chenxi Ye Chuanlai Yang Heqiang Zhang Rui Gao Yingnan Liao Yali Zhang Lingjun Jie Yanhui Zhang Tong Cheng Yan Wang Jie Ren Source Type: research
Hypoimmune islets achieve insulin independence after allogeneic transplantation in a fully immunocompetent non-human primate
We report that allogeneic transplantation of genetically engineered (B2M-/-, CIITA-/-, CD47+), primary, hypoimmune, pseudo-islets (p-islets) results in their engraftment into a fully immunocompetent, diabetic non-human primate wherein they provide stable endocrine function and enable insulin independence without inducing any detectable immune response in the absence of immunosuppression. Hypoimmune primary p-islets may provide a curative cell therapy for type 1 diabetes mellitus.PMID:38335966 | DOI:10.1016/j.stem.2024.02.001 (Source: Cell Stem Cell)
Source: Cell Stem Cell - February 9, 2024 Category: Stem Cells Authors: Xiaomeng Hu Kathy White Chi Young Ari G Olroyd Paul Kievit Andrew J Connolly Tobias Deuse Sonja Schrepfer Source Type: research
Hypoimmune islets achieve insulin independence after allogeneic transplantation in a fully immunocompetent non-human primate
We report that allogeneic transplantation of genetically engineered (B2M-/-, CIITA-/-, CD47+), primary, hypoimmune, pseudo-islets (p-islets) results in their engraftment into a fully immunocompetent, diabetic non-human primate wherein they provide stable endocrine function and enable insulin independence without inducing any detectable immune response in the absence of immunosuppression. Hypoimmune primary p-islets may provide a curative cell therapy for type 1 diabetes mellitus.PMID:38335966 | DOI:10.1016/j.stem.2024.02.001 (Source: Cell Stem Cell)
Source: Cell Stem Cell - February 9, 2024 Category: Stem Cells Authors: Xiaomeng Hu Kathy White Chi Young Ari G Olroyd Paul Kievit Andrew J Connolly Tobias Deuse Sonja Schrepfer Source Type: research
What is the scope of Cell Stem Cell now and in the future?
Cell Stem Cell. 2024 Feb 1;31(2):149-150. doi: 10.1016/j.stem.2024.01.005.NO ABSTRACTPMID:38306988 | DOI:10.1016/j.stem.2024.01.005 (Source: Cell Stem Cell)
Source: Cell Stem Cell - February 2, 2024 Category: Stem Cells Authors: Sheila Chari Catherine Landis Quan Wang Christine Weber Source Type: research
Bioprinting functional neural networks
Cell Stem Cell. 2024 Feb 1;31(2):151-152. doi: 10.1016/j.stem.2023.12.014.ABSTRACT3D printing human tissue models derived from stem cells provides an increasingly popular tissue engineering strategy for probing biological questions. Here Yan et al.1 demonstrate how this technology can be used to model mature human neural tissues with functional neural networks in healthy and disease states.PMID:38306989 | DOI:10.1016/j.stem.2023.12.014 (Source: Cell Stem Cell)
Source: Cell Stem Cell - February 2, 2024 Category: Stem Cells Authors: Stephanie M Willerth Source Type: research
Unraveling the brain's response to COVID-19: How SARS-CoV-2 afflicts dopaminergic neurons
Cell Stem Cell. 2024 Feb 1;31(2):152-154. doi: 10.1016/j.stem.2024.01.002.ABSTRACTCOVID-19 patients often display dysfunctions of the nervous system, indicating an effect of SARS-CoV-2 on neural cells. Yang et al. now show that human stem-cell-derived dopaminergic neurons are susceptible to SARS-CoV-2, triggering inflammation and senescence. The study further identifies three FDA-approved drugs capable of reversing these cellular phenotypes.PMID:38306990 | DOI:10.1016/j.stem.2024.01.002 (Source: Cell Stem Cell)
Source: Cell Stem Cell - February 2, 2024 Category: Stem Cells Authors: Ilse Eidhof Daniel Twohig Anna Falk Source Type: research
Extra (embryonic) dialogues: Keys to improved stem cell-based embryo models
Cell Stem Cell. 2024 Feb 1;31(2):155-157. doi: 10.1016/j.stem.2024.01.004.ABSTRACTDespite advances in stem cell cultures, modeling early human development with stem cells in a dish remains challenging. Research by Hislop et al.,1 Okubo et al.,2 and Wei et al.3 paves the way for improved in vitro embryo models and culture conditions, offering valuable insights for regenerative medicine.PMID:38306991 | DOI:10.1016/j.stem.2024.01.004 (Source: Cell Stem Cell)
Source: Cell Stem Cell - February 2, 2024 Category: Stem Cells Authors: Arun Pandian Chandrasekaran Mo Li Source Type: research
