An antimicrobial metabolite n- hexadecenoic acid from marine sponge-associated bacteria < em > Bacillus subtilis < /em > effectively inhibited biofilm forming multidrug-resistant < em > P. aeruginosa < /em >
Biofouling. 2023 Jul 24:1-14. doi: 10.1080/08927014.2023.2232722. Online ahead of print.ABSTRACTEffective drug candidates to obstruct the emergence of multidrug-resistant pathogens have become a major concern. A potent antimicrobial producer was isolated from a marine sponge designated as MSI38 and was identified as Bacillus subtilis by 16SrDNA sequencing. The active antimicrobial fraction was purified, and the metabolite was identified as n-hexadecanoic acid by spectroscopic analysis. The fish-borne pathogen Pseudomonas aeruginosa FP012 was found to be multidrug-resistant and poses a risk of disease to food handlers and c...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Arya Sajayan Amrudha Ravindran Joseph Selvin Prathiviraj Ragothaman George Seghal Kiran Source Type: research
Interspecies interactions in mixed-species biofilms formed by < em > Enterococcus faecalis < /em > and gram-negative bacteria isolated from polymicrobial diabetic foot ulcers
Biofouling. 2023 Jul 24:1-12. doi: 10.1080/08927014.2023.2236949. Online ahead of print.ABSTRACTDiabetic foot ulcers (DFU) are exacerbated by bacterial colonisation. Here, a high prevalence of Enterococcus faecalis was observed in DFU patients from an Argentinean hospital. E. faecalis was frequently co-isolated with Escherichia coli, Morganella morganii, and Pseudomonas aeruginosa. The effect of interspecies interactions on bacterial growth was investigated in mixed-species macrocolony biofilms developed in Lubbock-Glc-agar. Similar cell counts were found for E. faecalis and M. morganii growing in mixed and single-species ...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Florencia Mariani Guillermo E Juarez Claudia Barberis Florencia Veiga Carlos Vay Estela M Galvan Source Type: research
Thermal susceptibility and antibiotic synergism of methicillin-resistant < em > Staphylococcus aureus < /em > biofilms
This study investigated the thermal susceptibility of MRSA biofilms to thermal shocks from 60 to 80 °C for 1-30 min as well as the effect of various antibiotics (most notably methicillin) on thermal mitigation. Pre- and post-shock exposure to three different classes of antibiotics (ciprofloxacin, tobramycin, and methicillin) at concentrations ranging from 0.25 to 128 μg mL-1 were investigated. MRSA biofilms exhibited thermal susceptibility comparable to other common nosocomial pathogens, such as Pseudomonas aeruginosa, though with greater variability. Exposure to antibiotics of any class significantly decreased the degre...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Haydar A S Aljaafari Parham Parnian Jaymes Van Dyne Eric Nuxoll Source Type: research
An antimicrobial metabolite n- hexadecenoic acid from marine sponge-associated bacteria < em > Bacillus subtilis < /em > effectively inhibited biofilm forming multidrug-resistant < em > P. aeruginosa < /em >
Biofouling. 2023 Jul 24:1-14. doi: 10.1080/08927014.2023.2232722. Online ahead of print.ABSTRACTEffective drug candidates to obstruct the emergence of multidrug-resistant pathogens have become a major concern. A potent antimicrobial producer was isolated from a marine sponge designated as MSI38 and was identified as Bacillus subtilis by 16SrDNA sequencing. The active antimicrobial fraction was purified, and the metabolite was identified as n-hexadecanoic acid by spectroscopic analysis. The fish-borne pathogen Pseudomonas aeruginosa FP012 was found to be multidrug-resistant and poses a risk of disease to food handlers and c...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Arya Sajayan Amrudha Ravindran Joseph Selvin Prathiviraj Ragothaman George Seghal Kiran Source Type: research
Interspecies interactions in mixed-species biofilms formed by < em > Enterococcus faecalis < /em > and gram-negative bacteria isolated from polymicrobial diabetic foot ulcers
Biofouling. 2023 Jul 24:1-12. doi: 10.1080/08927014.2023.2236949. Online ahead of print.ABSTRACTDiabetic foot ulcers (DFU) are exacerbated by bacterial colonisation. Here, a high prevalence of Enterococcus faecalis was observed in DFU patients from an Argentinean hospital. E. faecalis was frequently co-isolated with Escherichia coli, Morganella morganii, and Pseudomonas aeruginosa. The effect of interspecies interactions on bacterial growth was investigated in mixed-species macrocolony biofilms developed in Lubbock-Glc-agar. Similar cell counts were found for E. faecalis and M. morganii growing in mixed and single-species ...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Florencia Mariani Guillermo E Juarez Claudia Barberis Florencia Veiga Carlos Vay Estela M Galvan Source Type: research
Thermal susceptibility and antibiotic synergism of methicillin-resistant < em > Staphylococcus aureus < /em > biofilms
This study investigated the thermal susceptibility of MRSA biofilms to thermal shocks from 60 to 80 °C for 1-30 min as well as the effect of various antibiotics (most notably methicillin) on thermal mitigation. Pre- and post-shock exposure to three different classes of antibiotics (ciprofloxacin, tobramycin, and methicillin) at concentrations ranging from 0.25 to 128 μg mL-1 were investigated. MRSA biofilms exhibited thermal susceptibility comparable to other common nosocomial pathogens, such as Pseudomonas aeruginosa, though with greater variability. Exposure to antibiotics of any class significantly decreased the degre...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Haydar A S Aljaafari Parham Parnian Jaymes Van Dyne Eric Nuxoll Source Type: research
An antimicrobial metabolite n- hexadecenoic acid from marine sponge-associated bacteria < em > Bacillus subtilis < /em > effectively inhibited biofilm forming multidrug-resistant < em > P. aeruginosa < /em >
Biofouling. 2023 Jul 24:1-14. doi: 10.1080/08927014.2023.2232722. Online ahead of print.ABSTRACTEffective drug candidates to obstruct the emergence of multidrug-resistant pathogens have become a major concern. A potent antimicrobial producer was isolated from a marine sponge designated as MSI38 and was identified as Bacillus subtilis by 16SrDNA sequencing. The active antimicrobial fraction was purified, and the metabolite was identified as n-hexadecanoic acid by spectroscopic analysis. The fish-borne pathogen Pseudomonas aeruginosa FP012 was found to be multidrug-resistant and poses a risk of disease to food handlers and c...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Arya Sajayan Amrudha Ravindran Joseph Selvin Prathiviraj Ragothaman George Seghal Kiran Source Type: research
Interspecies interactions in mixed-species biofilms formed by < em > Enterococcus faecalis < /em > and gram-negative bacteria isolated from polymicrobial diabetic foot ulcers
Biofouling. 2023 Jul 24:1-12. doi: 10.1080/08927014.2023.2236949. Online ahead of print.ABSTRACTDiabetic foot ulcers (DFU) are exacerbated by bacterial colonisation. Here, a high prevalence of Enterococcus faecalis was observed in DFU patients from an Argentinean hospital. E. faecalis was frequently co-isolated with Escherichia coli, Morganella morganii, and Pseudomonas aeruginosa. The effect of interspecies interactions on bacterial growth was investigated in mixed-species macrocolony biofilms developed in Lubbock-Glc-agar. Similar cell counts were found for E. faecalis and M. morganii growing in mixed and single-species ...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Florencia Mariani Guillermo E Juarez Claudia Barberis Florencia Veiga Carlos Vay Estela M Galvan Source Type: research
Thermal susceptibility and antibiotic synergism of methicillin-resistant < em > Staphylococcus aureus < /em > biofilms
This study investigated the thermal susceptibility of MRSA biofilms to thermal shocks from 60 to 80 °C for 1-30 min as well as the effect of various antibiotics (most notably methicillin) on thermal mitigation. Pre- and post-shock exposure to three different classes of antibiotics (ciprofloxacin, tobramycin, and methicillin) at concentrations ranging from 0.25 to 128 μg mL-1 were investigated. MRSA biofilms exhibited thermal susceptibility comparable to other common nosocomial pathogens, such as Pseudomonas aeruginosa, though with greater variability. Exposure to antibiotics of any class significantly decreased the degre...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Haydar A S Aljaafari Parham Parnian Jaymes Van Dyne Eric Nuxoll Source Type: research
Pyrrole-2-carboxylic acid inhibits biofilm formation and suppresses the virulence of < em > Listeria monocytogenes < /em >
This study aimed to explore the antibiofilm efficacy of pyrrole-2-carboxylic acid (PCA) against L. monocytogenes. Crystal violet staining assay demonstrated that PCA reduced the biofilm biomass of L. monocytogenes. The 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide reduction and flow cytometric assays indicated that PCA attenuated the metabolic activity of L. monocytogenes biofilm together with a decrease in viability. Morphologic observations revealed that PCA exposure collapsed the biofilm architecture. PCA administration of 0.75 mg ml-1 decreased the excretion of extracellular DNA, protein and ...
Source: Biofouling - July 21, 2023 Category: Molecular Biology Authors: Yuxi Yue Kai Zhong Yanping Wu Hong Gao Source Type: research
The combined effect of essential oils on wood physico-chemical properties and their antiadhesive activity against mold fungi: application of mixture design methodology
Biofouling. 2023 Jul 21:1-18. doi: 10.1080/08927014.2023.2236029. Online ahead of print.ABSTRACTIn the heritage field, the microbial adhesion on wood, and consequently the formation of biofilm led to inestimable losses of historical and cultural monuments. Thereby, this study aimed to examine the combined effect of Thymus vulgaris, Myrtus communis, and Mentha pulegium essential oils on wood surface physico-chemical properties, and to elaborate the optimal mixture using the mixture design approach coupled to the contact angle method. It was found that both wood hydrophobicity and electron donor character increased significa...
Source: Biofouling - July 21, 2023 Category: Molecular Biology Authors: Moulay Sadiki Mounyr Balouiri Soumya Elabed Fadoua Bennouna Mohammed Lachkar Saad Ibnsouda Koraichi Source Type: research
Pyrrole-2-carboxylic acid inhibits biofilm formation and suppresses the virulence of < em > Listeria monocytogenes < /em >
This study aimed to explore the antibiofilm efficacy of pyrrole-2-carboxylic acid (PCA) against L. monocytogenes. Crystal violet staining assay demonstrated that PCA reduced the biofilm biomass of L. monocytogenes. The 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide reduction and flow cytometric assays indicated that PCA attenuated the metabolic activity of L. monocytogenes biofilm together with a decrease in viability. Morphologic observations revealed that PCA exposure collapsed the biofilm architecture. PCA administration of 0.75 mg ml-1 decreased the excretion of extracellular DNA, protein and ...
Source: Biofouling - July 21, 2023 Category: Molecular Biology Authors: Yuxi Yue Kai Zhong Yanping Wu Hong Gao Source Type: research
The combined effect of essential oils on wood physico-chemical properties and their antiadhesive activity against mold fungi: application of mixture design methodology
Biofouling. 2023 Jul 21:1-18. doi: 10.1080/08927014.2023.2236029. Online ahead of print.ABSTRACTIn the heritage field, the microbial adhesion on wood, and consequently the formation of biofilm led to inestimable losses of historical and cultural monuments. Thereby, this study aimed to examine the combined effect of Thymus vulgaris, Myrtus communis, and Mentha pulegium essential oils on wood surface physico-chemical properties, and to elaborate the optimal mixture using the mixture design approach coupled to the contact angle method. It was found that both wood hydrophobicity and electron donor character increased significa...
Source: Biofouling - July 21, 2023 Category: Molecular Biology Authors: Moulay Sadiki Mounyr Balouiri Soumya Elabed Fadoua Bennouna Mohammed Lachkar Saad Ibnsouda Koraichi Source Type: research
Pyrrole-2-carboxylic acid inhibits biofilm formation and suppresses the virulence of < em > Listeria monocytogenes < /em >
This study aimed to explore the antibiofilm efficacy of pyrrole-2-carboxylic acid (PCA) against L. monocytogenes. Crystal violet staining assay demonstrated that PCA reduced the biofilm biomass of L. monocytogenes. The 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide reduction and flow cytometric assays indicated that PCA attenuated the metabolic activity of L. monocytogenes biofilm together with a decrease in viability. Morphologic observations revealed that PCA exposure collapsed the biofilm architecture. PCA administration of 0.75 mg ml-1 decreased the excretion of extracellular DNA, protein and ...
Source: Biofouling - July 21, 2023 Category: Molecular Biology Authors: Yuxi Yue Kai Zhong Yanping Wu Hong Gao Source Type: research
The combined effect of essential oils on wood physico-chemical properties and their antiadhesive activity against mold fungi: application of mixture design methodology
Biofouling. 2023 Jul 21:1-18. doi: 10.1080/08927014.2023.2236029. Online ahead of print.ABSTRACTIn the heritage field, the microbial adhesion on wood, and consequently the formation of biofilm led to inestimable losses of historical and cultural monuments. Thereby, this study aimed to examine the combined effect of Thymus vulgaris, Myrtus communis, and Mentha pulegium essential oils on wood surface physico-chemical properties, and to elaborate the optimal mixture using the mixture design approach coupled to the contact angle method. It was found that both wood hydrophobicity and electron donor character increased significa...
Source: Biofouling - July 21, 2023 Category: Molecular Biology Authors: Moulay Sadiki Mounyr Balouiri Soumya Elabed Fadoua Bennouna Mohammed Lachkar Saad Ibnsouda Koraichi Source Type: research
