Interspecies interactions in mixed-species biofilms formed by < em > Enterococcus faecalis < /em > and gram-negative bacteria isolated from polymicrobial diabetic foot ulcers
Biofouling. 2023 May-Jul;39(5):579-590. doi: 10.1080/08927014.2023.2236949. Epub 2023 Jul 24.ABSTRACTDiabetic foot ulcers (DFU) are exacerbated by bacterial colonisation. Here, a high prevalence of Enterococcus faecalis was observed in DFU patients from an Argentinean hospital. E. faecalis was frequently co-isolated with Escherichia coli, Morganella morganii, and Pseudomonas aeruginosa. The effect of interspecies interactions on bacterial growth was investigated in mixed-species macrocolony biofilms developed in Lubbock-Glc-agar. Similar cell counts were found for E. faecalis and M. morganii growing in mixed and single-spe...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Florencia Mariani Guillermo E Juarez Claudia Barberis Florencia Veiga Carlos Vay Estela M Galvan Source Type: research
Thermal susceptibility and antibiotic synergism of methicillin-resistant < em > Staphylococcus aureus < /em > biofilms
This study investigated the thermal susceptibility of MRSA biofilms to thermal shocks from 60 to 80 °C for 1-30 min as well as the effect of various antibiotics (most notably methicillin) on thermal mitigation. Pre- and post-shock exposure to three different classes of antibiotics (ciprofloxacin, tobramycin, and methicillin) at concentrations ranging from 0.25 to 128 μg mL-1 were investigated. MRSA biofilms exhibited thermal susceptibility comparable to other common nosocomial pathogens, such as Pseudomonas aeruginosa, though with greater variability. Exposure to antibiotics of any class significantly decreased the degre...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Haydar A S Aljaafari Parham Parnian Jaymes Van Dyne Eric Nuxoll Source Type: research
An antimicrobial metabolite n- hexadecenoic acid from marine sponge-associated bacteria < em > Bacillus subtilis < /em > effectively inhibited biofilm forming multidrug-resistant < em > P. aeruginosa < /em >
Biofouling. 2023 May-Jul;39(5):502-515. doi: 10.1080/08927014.2023.2232722. Epub 2023 Jul 24.ABSTRACTEffective drug candidates to obstruct the emergence of multidrug-resistant pathogens have become a major concern. A potent antimicrobial producer was isolated from a marine sponge designated as MSI38 and was identified as Bacillus subtilis by 16SrDNA sequencing. The active antimicrobial fraction was purified, and the metabolite was identified as n-hexadecanoic acid by spectroscopic analysis. The fish-borne pathogen Pseudomonas aeruginosa FP012 was found to be multidrug-resistant and poses a risk of disease to food handlers ...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Arya Sajayan Amrudha Ravindran Joseph Selvin Prathiviraj Ragothaman George Seghal Kiran Source Type: research
Interspecies interactions in mixed-species biofilms formed by < em > Enterococcus faecalis < /em > and gram-negative bacteria isolated from polymicrobial diabetic foot ulcers
Biofouling. 2023 May-Jul;39(5):579-590. doi: 10.1080/08927014.2023.2236949. Epub 2023 Jul 24.ABSTRACTDiabetic foot ulcers (DFU) are exacerbated by bacterial colonisation. Here, a high prevalence of Enterococcus faecalis was observed in DFU patients from an Argentinean hospital. E. faecalis was frequently co-isolated with Escherichia coli, Morganella morganii, and Pseudomonas aeruginosa. The effect of interspecies interactions on bacterial growth was investigated in mixed-species macrocolony biofilms developed in Lubbock-Glc-agar. Similar cell counts were found for E. faecalis and M. morganii growing in mixed and single-spe...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Florencia Mariani Guillermo E Juarez Claudia Barberis Florencia Veiga Carlos Vay Estela M Galvan Source Type: research
Thermal susceptibility and antibiotic synergism of methicillin-resistant < em > Staphylococcus aureus < /em > biofilms
This study investigated the thermal susceptibility of MRSA biofilms to thermal shocks from 60 to 80 °C for 1-30 min as well as the effect of various antibiotics (most notably methicillin) on thermal mitigation. Pre- and post-shock exposure to three different classes of antibiotics (ciprofloxacin, tobramycin, and methicillin) at concentrations ranging from 0.25 to 128 μg mL-1 were investigated. MRSA biofilms exhibited thermal susceptibility comparable to other common nosocomial pathogens, such as Pseudomonas aeruginosa, though with greater variability. Exposure to antibiotics of any class significantly decreased the degre...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Haydar A S Aljaafari Parham Parnian Jaymes Van Dyne Eric Nuxoll Source Type: research
An antimicrobial metabolite n- hexadecenoic acid from marine sponge-associated bacteria < em > Bacillus subtilis < /em > effectively inhibited biofilm forming multidrug-resistant < em > P. aeruginosa < /em >
Biofouling. 2023 May-Jul;39(5):502-515. doi: 10.1080/08927014.2023.2232722. Epub 2023 Jul 24.ABSTRACTEffective drug candidates to obstruct the emergence of multidrug-resistant pathogens have become a major concern. A potent antimicrobial producer was isolated from a marine sponge designated as MSI38 and was identified as Bacillus subtilis by 16SrDNA sequencing. The active antimicrobial fraction was purified, and the metabolite was identified as n-hexadecanoic acid by spectroscopic analysis. The fish-borne pathogen Pseudomonas aeruginosa FP012 was found to be multidrug-resistant and poses a risk of disease to food handlers ...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Arya Sajayan Amrudha Ravindran Joseph Selvin Prathiviraj Ragothaman George Seghal Kiran Source Type: research
Interspecies interactions in mixed-species biofilms formed by < em > Enterococcus faecalis < /em > and gram-negative bacteria isolated from polymicrobial diabetic foot ulcers
Biofouling. 2023 May-Jul;39(5):579-590. doi: 10.1080/08927014.2023.2236949. Epub 2023 Jul 24.ABSTRACTDiabetic foot ulcers (DFU) are exacerbated by bacterial colonisation. Here, a high prevalence of Enterococcus faecalis was observed in DFU patients from an Argentinean hospital. E. faecalis was frequently co-isolated with Escherichia coli, Morganella morganii, and Pseudomonas aeruginosa. The effect of interspecies interactions on bacterial growth was investigated in mixed-species macrocolony biofilms developed in Lubbock-Glc-agar. Similar cell counts were found for E. faecalis and M. morganii growing in mixed and single-spe...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Florencia Mariani Guillermo E Juarez Claudia Barberis Florencia Veiga Carlos Vay Estela M Galvan Source Type: research
Thermal susceptibility and antibiotic synergism of methicillin-resistant < em > Staphylococcus aureus < /em > biofilms
This study investigated the thermal susceptibility of MRSA biofilms to thermal shocks from 60 to 80 °C for 1-30 min as well as the effect of various antibiotics (most notably methicillin) on thermal mitigation. Pre- and post-shock exposure to three different classes of antibiotics (ciprofloxacin, tobramycin, and methicillin) at concentrations ranging from 0.25 to 128 μg mL-1 were investigated. MRSA biofilms exhibited thermal susceptibility comparable to other common nosocomial pathogens, such as Pseudomonas aeruginosa, though with greater variability. Exposure to antibiotics of any class significantly decreased the degre...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Haydar A S Aljaafari Parham Parnian Jaymes Van Dyne Eric Nuxoll Source Type: research
An antimicrobial metabolite n- hexadecenoic acid from marine sponge-associated bacteria < em > Bacillus subtilis < /em > effectively inhibited biofilm forming multidrug-resistant < em > P. aeruginosa < /em >
Biofouling. 2023 Jul 24:1-14. doi: 10.1080/08927014.2023.2232722. Online ahead of print.ABSTRACTEffective drug candidates to obstruct the emergence of multidrug-resistant pathogens have become a major concern. A potent antimicrobial producer was isolated from a marine sponge designated as MSI38 and was identified as Bacillus subtilis by 16SrDNA sequencing. The active antimicrobial fraction was purified, and the metabolite was identified as n-hexadecanoic acid by spectroscopic analysis. The fish-borne pathogen Pseudomonas aeruginosa FP012 was found to be multidrug-resistant and poses a risk of disease to food handlers and c...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Arya Sajayan Amrudha Ravindran Joseph Selvin Prathiviraj Ragothaman George Seghal Kiran Source Type: research
Interspecies interactions in mixed-species biofilms formed by < em > Enterococcus faecalis < /em > and gram-negative bacteria isolated from polymicrobial diabetic foot ulcers
Biofouling. 2023 Jul 24:1-12. doi: 10.1080/08927014.2023.2236949. Online ahead of print.ABSTRACTDiabetic foot ulcers (DFU) are exacerbated by bacterial colonisation. Here, a high prevalence of Enterococcus faecalis was observed in DFU patients from an Argentinean hospital. E. faecalis was frequently co-isolated with Escherichia coli, Morganella morganii, and Pseudomonas aeruginosa. The effect of interspecies interactions on bacterial growth was investigated in mixed-species macrocolony biofilms developed in Lubbock-Glc-agar. Similar cell counts were found for E. faecalis and M. morganii growing in mixed and single-species ...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Florencia Mariani Guillermo E Juarez Claudia Barberis Florencia Veiga Carlos Vay Estela M Galvan Source Type: research
Thermal susceptibility and antibiotic synergism of methicillin-resistant < em > Staphylococcus aureus < /em > biofilms
This study investigated the thermal susceptibility of MRSA biofilms to thermal shocks from 60 to 80 °C for 1-30 min as well as the effect of various antibiotics (most notably methicillin) on thermal mitigation. Pre- and post-shock exposure to three different classes of antibiotics (ciprofloxacin, tobramycin, and methicillin) at concentrations ranging from 0.25 to 128 μg mL-1 were investigated. MRSA biofilms exhibited thermal susceptibility comparable to other common nosocomial pathogens, such as Pseudomonas aeruginosa, though with greater variability. Exposure to antibiotics of any class significantly decreased the degre...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Haydar A S Aljaafari Parham Parnian Jaymes Van Dyne Eric Nuxoll Source Type: research
An antimicrobial metabolite n- hexadecenoic acid from marine sponge-associated bacteria < em > Bacillus subtilis < /em > effectively inhibited biofilm forming multidrug-resistant < em > P. aeruginosa < /em >
Biofouling. 2023 Jul 24:1-14. doi: 10.1080/08927014.2023.2232722. Online ahead of print.ABSTRACTEffective drug candidates to obstruct the emergence of multidrug-resistant pathogens have become a major concern. A potent antimicrobial producer was isolated from a marine sponge designated as MSI38 and was identified as Bacillus subtilis by 16SrDNA sequencing. The active antimicrobial fraction was purified, and the metabolite was identified as n-hexadecanoic acid by spectroscopic analysis. The fish-borne pathogen Pseudomonas aeruginosa FP012 was found to be multidrug-resistant and poses a risk of disease to food handlers and c...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Arya Sajayan Amrudha Ravindran Joseph Selvin Prathiviraj Ragothaman George Seghal Kiran Source Type: research
Interspecies interactions in mixed-species biofilms formed by < em > Enterococcus faecalis < /em > and gram-negative bacteria isolated from polymicrobial diabetic foot ulcers
Biofouling. 2023 Jul 24:1-12. doi: 10.1080/08927014.2023.2236949. Online ahead of print.ABSTRACTDiabetic foot ulcers (DFU) are exacerbated by bacterial colonisation. Here, a high prevalence of Enterococcus faecalis was observed in DFU patients from an Argentinean hospital. E. faecalis was frequently co-isolated with Escherichia coli, Morganella morganii, and Pseudomonas aeruginosa. The effect of interspecies interactions on bacterial growth was investigated in mixed-species macrocolony biofilms developed in Lubbock-Glc-agar. Similar cell counts were found for E. faecalis and M. morganii growing in mixed and single-species ...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Florencia Mariani Guillermo E Juarez Claudia Barberis Florencia Veiga Carlos Vay Estela M Galvan Source Type: research
Thermal susceptibility and antibiotic synergism of methicillin-resistant < em > Staphylococcus aureus < /em > biofilms
This study investigated the thermal susceptibility of MRSA biofilms to thermal shocks from 60 to 80 °C for 1-30 min as well as the effect of various antibiotics (most notably methicillin) on thermal mitigation. Pre- and post-shock exposure to three different classes of antibiotics (ciprofloxacin, tobramycin, and methicillin) at concentrations ranging from 0.25 to 128 μg mL-1 were investigated. MRSA biofilms exhibited thermal susceptibility comparable to other common nosocomial pathogens, such as Pseudomonas aeruginosa, though with greater variability. Exposure to antibiotics of any class significantly decreased the degre...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Haydar A S Aljaafari Parham Parnian Jaymes Van Dyne Eric Nuxoll Source Type: research
An antimicrobial metabolite n- hexadecenoic acid from marine sponge-associated bacteria < em > Bacillus subtilis < /em > effectively inhibited biofilm forming multidrug-resistant < em > P. aeruginosa < /em >
Biofouling. 2023 Jul 24:1-14. doi: 10.1080/08927014.2023.2232722. Online ahead of print.ABSTRACTEffective drug candidates to obstruct the emergence of multidrug-resistant pathogens have become a major concern. A potent antimicrobial producer was isolated from a marine sponge designated as MSI38 and was identified as Bacillus subtilis by 16SrDNA sequencing. The active antimicrobial fraction was purified, and the metabolite was identified as n-hexadecanoic acid by spectroscopic analysis. The fish-borne pathogen Pseudomonas aeruginosa FP012 was found to be multidrug-resistant and poses a risk of disease to food handlers and c...
Source: Biofouling - July 24, 2023 Category: Molecular Biology Authors: Arya Sajayan Amrudha Ravindran Joseph Selvin Prathiviraj Ragothaman George Seghal Kiran Source Type: research
