14-Deoxygarcinol improves insulin sensitivity in high-fat diet-induced obese mice via mitigating NF- κB/Sirtuin 2-NLRP3-mediated adipose tissue remodeling
In this study we investigated whether 14-deoxygarcinol (DOG), a major polyisoprenylated benzophenone from brindle berry, alleviated adipose tissue inflammation and insulin sensitivity in high-fat diet fed mice. The mice were administered DOG (2.5, 5 mg · kg-1 · d-1, i.p.) for 4 weeks. We showed that DOG injection dose-dependently improved insulin resistance and hyperlipidemia, but not adiposity in high-fat diet-fed mice. We found that DOG injection significantly alleviated adipose tissue inflammation via preventing macrophage infiltration and pro-inflammatory polarization of macrophages, and adipose tissue fibrosis via r...
Source: Acta Pharmacologica Sinica - August 9, 2022 Category: Drugs & Pharmacology Authors: Jia-Li Chen Zhe-Ling Feng Fei Zhou Ruo-Han Lou Cheng Peng Yang Ye Li-Gen Lin Source Type: research
Development and Validation of an Analytical Method to Quantitate Hydroxycitric Acid, the Key Constituent in < em > Garcinia cambogia < /em > Extract, in Rodent Plasma and Fetus
We report a validated method utilizing sample clean up with ultrafiltration followed by liquid chromatography-tandem mass spectrometry analysis to quantify HCA in rat plasma over the concentration range of 20 to 800 ng/mL. The method was linear (r2 ≥ 0.99) with the limits of quantitation (LOQ) and detection (LOD) of 20.0 and 3.9 ng/mL plasma, respectively. The accuracy (determined as relative error, RE) and precision (determined as relative standard deviation, RSD) using Quality Control standards analyzed over multiple days were ≤ ± 7.5% and ≤ 9.5%, respectively. The method can be applied to quantify HCA in study ma...
Source: Analytical Letters - April 14, 2022 Category: Chemistry Authors: Esra Mutlu Jessica Pierfelice Yu Cao Alison Djonabaye Shelly Gleason Brian Burback Suramya Waidyanatha Source Type: research
