MicroRNA Signature and Cellular Characterization of Undifferentiated and Differentiated House Ear Institute-Organ of Corti 1 (HEI-OC1) Cells

This study therefore aimed to compare the miRNA profiles and cellular characteristics of HEI-OC1 cells cultured under permissive (P-HEI-OC1) and non-permissive (NP-HEI-OC1) conditions. A significant increase in the level of expression of tubulin β1 class VI(Tubb1), e-cadherin(Cdh1), espin(Espn), and SRY (sex determining region Y)-box2(Sox2) mRNAs was identified in non-permissive cells compared with permissive cells (P <  0.05, Kruskal–Wallis H test, 2-sided). miR-200 family, miR-34b/c, and miR-449a/b functionally related cluster miRNAs, rodent-specific maternally imprinted geneSfmbt2 intron 10th cluster miRNAs (-466a/ -467a), and miR-17 family were significantly (P <  0.05, Welch’st-test, 2-tailed) differentially expressed in non-permissive cells when compared with permissive cells. Putative target genes were significantly predominantly enriched in mitogen-activated protein kinase (MAPK), epidermal growth factor family of receptor tyrosine kinases (ErbB), and Ras signaling pathways in non-permissive cells compared with permissive cells. This distinct miRNA signature of differentiated HEI-OC1 cells could help in understanding miRNA-mediated cellular responses in the adult cochlea.

Source: JARO - Journal of the Association for Research in Otolaryngology - May 11, 2022 Category: ENT & OMF Source Type: research