SPATA33 affects the formation of cell adhesion complex by interacting with CTNNA3 in TM4 cells

In this study, we found that SPATA33 and CTNNA3 were involved in this process. Quantitative real-time PCR and western blotting showed similar trend of expression of two proteins in the testis of mice of different ages. Subsequently, CRISPR-Cas9 technique was used to prepareSpata33 knockout cell lines with TM4 cells, cell wound scratch assay showed thatSpata33 gene knockout affected cell migration, and flow cytometry assay showed thatSpata33 knockout resulted in a decreased percentage of G1 phase cells in TM4 cell line. In addition, phalloidin staining assay showed thatSpata33 gene knockout disrupted the formation of F-actin. Moreover, the protein immunoprecipitation experiment showed the interaction between SPATA33 and CTNNA3, which affected the interaction between CTNNA3 and CTNNB1. SPATA33 inhibits the formation of CDH1-CTNNB1-CTNNA3 complex through its interaction with CTNNA3, thus weakening adhesion between Sertoli cell and promoting cell migration.

Source: Cell and Tissue Research - May 10, 2022 Category: Cytology Source Type: research