New cytotoxic premyrsinane type diterpenes from Euphorbia aleppica against breast cancer cells

Introduction: Euphorbia aleppica from the Euphorbiaceae family was collected from Kuhdasht, Lorestan, Iran. Euphorbia has been used in traditional medicine to remove warts, possibly due to its cytotoxic or antiviral effects. Therefore, this study investigates its phytochemistry and isolates its bioactive compounds.Method: Plant material was dried and ground. Extraction was performed by maceration using dichloromethane: acetone solvent. After defating, fractionation was done by open column chromatography. Based on the initial H-NMR spectra, fractions containing diterpenoid compounds were identified. Isolation was performed by the Sephadex column and HPLC. HPLC was done on a normal YMC silica column using hexane: ethyl acetate (70:30) as solvent. The selected sub-fractions were identified by one, and two-dimensional corelative NMR spectra. Accurate mass spectra confirmed the molecular formula of the obtained structures. Cytotoxicity was assessed using a standard MTT assay against breast cancer cells.Results and discussion: Based on NMR and Mass analysis, compound 1 as a new described and compound 2 as a known compound were identified as 3,7,15β- triacetyl-5α-tigliate-13(17)-α-epoxy-14-oxopremyrsinane, and 3,7,14,15,17-pentaacetyl-5-tigliate-13(17)-epoxypremyrsinane, respectively. Compound 1 showed moderate cytotoxicity, and compound 2 exhibited a potent cytotoxic effect in a dose-dependent manner against MCF-7 and MDA-MB 231 breast cancer cells, pro...
Source: Iranian Journal of Pharmaceutical Research - Category: Drugs & Pharmacology Source Type: research