Isolation of Heartland Virus from Lone Star Ticks, Georgia, USA, 2019

Emerg Infect Dis. 2022 Apr;28(4):786-792. doi: 10.3201/eid2804.211540.ABSTRACTReport of a human death and exposure of white-tailed deer to Heartland virus (HRTV) in Georgia, USA, prompted the sampling of questing ticks during 2018-2019 in 26 sites near where seropositive deer were captured and the residence of the human case-patient. We processed 9,294 Amblyomma americanum ticks in pools by virus isolation in Vero E6 cells and reverse transcription PCR. Positive pools underwent whole-genome sequencing. Three pools were positive for HRTV (minimum infection rate 0.46/1,000 ticks) and none for Bourbon virus. Cell cultures confirmed HRTV presence in 2 pools. Genome sequencing, achieved for the 3 HRTV isolates, showed high similarity among samples but marked differences with previously sequenced HRTV isolates. The isolation and genomic characterization of HRTV from A. americanum ticks in Georgia confirm virus presence in the state. Clinicians and public health professionals should be aware of this emerging tickborne pathogen.PMID:35318917 | DOI:10.3201/eid2804.211540

Source: Emerging Infectious Diseases - March 23, 2022 Category: Infectious Diseases Authors: Yamila Romer Kayla Adcock Zhuoran Wei Daniel G Mead Oscar Kirstein Steph Bellman Anne Piantadosi Uriel Kitron Gonzalo M Vazquez-Prokopec Source Type: research