Expression, purification, crystallization and preliminary X-ray diffraction analysis of a type II NADH:quinone oxidoreductase from the human pathogen Staphylococcus aureus

In recent years, type II NADH dehydrogenases (NDH-IIs) have emerged as potential drug targets for a wide range of human disease causative agents. In this work, the NDH-II enzyme from the Gram-positive human pathogen Staphylococcus aureus was recombinantly expressed in Escherichia coli, purified, crystallized and a crystallographic data set was collected at a wavelength of 0.873 Å. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 81.8, b = 86.0, c = 269.9 Å, contained four monomers per asymmetric unit and diffracted to a resolution of 3.32 Å. A molecular-replacement solution was obtained and model building and refinement are currently under way.

Source: Acta Crystallographica Section F - March 27, 2015 Category: Biochemistry Authors: Rosário, A.L.Sena, F.V.Batista, A.P.Oliveira, T.F.Athayde, D.Pereira, M.M.Brito, J.A.Archer, M. Tags: NDH-II NADH dehydrogenase respiratory chain Staphylococcus aureus research communications Source Type: research