Skeletal Muscle MiR-210 Expression is Associated with Mitochondrial Function in Peripheral Artery Disease Patients

Transl Res. 2022 Mar 11:S1931-5244(22)00046-9. doi: 10.1016/j.trsl.2022.03.003. Online ahead of print.ABSTRACTPrevious studies have demonstrated that circulating microRNA (miR)-210 levels are elevated in peripheral artery disease (PAD) patients. MiR-210 is known to be a negative regulator of mitochondrial respiration; however, the relationship between miR-210 and mitochondrial function has yet to be studied in PAD. We aimed to compare skeletal muscle miR-210 expression of PAD patients to non-PAD controls (CON) and to examine the relationship between miR-210 expression and mitochondrial function. Skeletal muscle biopsies from CON (n=20), intermittent claudication (IC) patients (n=20), and critical limb ischemia (CLI) patients (n=20) were analyzed by high-resolution respirometry to measure mitochondrial respiration of permeabilized fibers. Samples were also analyzed for miR-210 expression by real-time PCR. MiR-210 expression was significantly elevated in IC and CLI muscle compared to CON (p=0.008 and p<0.001, respectively). Mitochondrial respiration of electron transport chain (ETC) Complexes II (p=0.001) and IV (p<0.001) were significantly reduced in IC patients. Further, CLI patients demonstrated significant reductions in respiration during Complexes I (state 2: p=0.04, state 3: p=0.003), combined I and II (p<0.001), II (p<0.001), and IV (p<0.001). The expression of the miR-210 targets, cytochrome c oxidase assembly factor heme A:farnesyltransferase (COX10) and...
Source: Translational Research : the journal of laboratory and clinical medicine - Category: Laboratory Medicine Authors: Source Type: research