P8.08 * Roles of Nectin-4 in tumor progression

Nectin-4 is a transmembrane immunoglobulin weakly expressed in healthy tissues, in contrast with embryonic stage. Interestingly, an expression of nectin-4 is found in the breast, ovarian and lung tumors. This expression is correlated to the advanced stages of these cancerous pathologies. Furthermore, high levels of plasmatic form is also found at an important rate in the advanced stages of breast, ovarian and lung carcinomas. To identify the roles of nectin-4 expression in tumor progression, we used in our study MCF-7 & BT-474 cell lines expressing an endogenous nectin-4, but also MDA-MB213 cell lines transfected with native forms (MDA-MB213 R4. C1) or with a form of nectin-4 truncate of eight amino acids at juxta-membrane domain (MDA-MB231 Del8.6). We observed that the MDA-MB231 R4.C1 and MDA-MB231 Del8.6 cells, have high growth capacities. But this growth seems more important in drastic medium conditions, only for MDA-MB231 R4.C1 cells that produce a soluble form. Using monoclonal antibodies targeting the extracellular domain of nectin-4, we observe significant diminution of cell growth for BT-474 & MCF-7 cell lines. Similar results are obtained by using a specific siRNA. An implication of nectin-4 in the increase of the cellular migration is also observed. Indeed, cells expressed nectin-4 show a migration capability higher than about 66% compared with MDA-MB231 wt cells and it seems that the soluble form does not influence the intensity of cell migration.The use of...
Source: Annals of Oncology - Category: Cancer & Oncology Authors: Tags: Poster session 8: Other topics Source Type: research