Endothelial Cell Fibrin Gel Angiogenesis Bead Assay

Methods Mol Biol. 2022;2441:321-327. doi: 10.1007/978-1-0716-2059-5_25.ABSTRACTThe fibrin gel angiogenesis bead assay provides a controlled in vitro setting for observing endothelial angiogenic sprouting in response to modified variables. Endothelial cells are coated onto microcarriers and embedded into a fibrin clot containing necessary growth factors. Following a 24-h incubation, endothelial sprouts are imaged using a light microscope. This method is useful for rapidly and affordably investigating the effects of genetic or chemical manipulation to endothelial function.PMID:35099748 | DOI:10.1007/978-1-0716-2059-5_25

Source: Mol Biol Cell - January 31, 2022 Category: Molecular Biology Authors: Eva M Clavane Hannah A Taylor Richard M Cubbon Paul J Meakin Source Type: research